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The map label for this gene is nfo

Identifier: 83589972

GI number: 83589972

Start: 1152665

End: 1153504

Strand: Reverse

Name: nfo

Synonym: Moth_1124

Alternate gene names: 83589972

Gene position: 1153504-1152665 (Counterclockwise)

Preceding gene: 83589973

Following gene: 83589970

Centisome position: 43.88

GC content: 55.71

Gene sequence:

>840_bases
ATGCGTCTTGGAGCCCATCTATCCATAGCTAAAGGCCTACCAAGGACTGCAGCCATGGCCACTAGCATAGGAGCCAACAC
CTTCCAGTATTTTACGCGCAACCCCCGCGGCGGGGCCGCCAGGCAGATTCCCGGCAAAGAGATCCAGGCCTGGAGGGAGG
CAAGACGCCGGGCCGATCTTTATCCCATCGCCGGCCATTTGCCTTATACCGTAAACCTGGGCGCCGCCGCTGAAAGACAA
CAGGAATTTACCCGTATGGTTCTTCATGATGATACCCTGCGGGTGGCAGCCATAGACGGCGAATACCTGATCAGCCATCC
CGGCCACTATGAGGGAGAACGCCAGGCCGGCCTAGACAGAATCATCCAGTTGATCGAGGAAGCCTATTTGAGCATTACCC
CTCCGGGTCCCATGCTTTTACTGGAAACTATGGCCGGCCAGGGAAAAGAAGTGGGTACCATTGATGATCTGTGCTATATT
CTCGAGGGCCTAGGATGGCCGGATAGAGTAGGAGTATGCCTGGACTCGGCCCATCTGTTTGCCGCCGGCTGGGACCTGCG
TACCCCGGCAGGTTGCCAGCAACTGGTACAAGAATTGGCCGCAAAAATTGGCCTGGACCGGGTTAAGGCCATGCACCTCA
ATGATTCCGCCGCACCCCTTGGTAGCCACCGGGACCGCCATGCCGGAATCGGCAAGGGCGAGCTGGGAAGGGAAGGCATA
GCGGCGGTAGTTAATGATCCTTTTCTGGGGGAGCTGCCCTTATTTTTAGAAACTCCAGTTGCCAATTACGAAGAATATGG
TGAGGAAATTGCCCTGATCCAAAAACTAAAATCCGTTTAG

Upstream 100 bases:

>100_bases
CAGCCGACGAAACCACGGGATTGGCACTTATTGAAGCAAGCGACTTGGTCTGGAAGAAAACTAAGCGTGCATAATCAGAG
TTATTTGCGGAGGGAAAAAC

Downstream 100 bases:

>100_bases
CTTGCTTCTCCCTGCAGCTATATTTCAAACTGAGGGATCGCGGGGTATTGACGATGTCTGGATGTCTATGATATAATAGG
CTTCGGTTGAGGCCCTATCG

Product: endonuclease IV

Products: NA

Alternate protein names: Endodeoxyribonuclease IV; Endonuclease IV

Number of amino acids: Translated: 279; Mature: 279

Protein sequence:

>279_residues
MRLGAHLSIAKGLPRTAAMATSIGANTFQYFTRNPRGGAARQIPGKEIQAWREARRRADLYPIAGHLPYTVNLGAAAERQ
QEFTRMVLHDDTLRVAAIDGEYLISHPGHYEGERQAGLDRIIQLIEEAYLSITPPGPMLLLETMAGQGKEVGTIDDLCYI
LEGLGWPDRVGVCLDSAHLFAAGWDLRTPAGCQQLVQELAAKIGLDRVKAMHLNDSAAPLGSHRDRHAGIGKGELGREGI
AAVVNDPFLGELPLFLETPVANYEEYGEEIALIQKLKSV

Sequences:

>Translated_279_residues
MRLGAHLSIAKGLPRTAAMATSIGANTFQYFTRNPRGGAARQIPGKEIQAWREARRRADLYPIAGHLPYTVNLGAAAERQ
QEFTRMVLHDDTLRVAAIDGEYLISHPGHYEGERQAGLDRIIQLIEEAYLSITPPGPMLLLETMAGQGKEVGTIDDLCYI
LEGLGWPDRVGVCLDSAHLFAAGWDLRTPAGCQQLVQELAAKIGLDRVKAMHLNDSAAPLGSHRDRHAGIGKGELGREGI
AAVVNDPFLGELPLFLETPVANYEEYGEEIALIQKLKSV
>Mature_279_residues
MRLGAHLSIAKGLPRTAAMATSIGANTFQYFTRNPRGGAARQIPGKEIQAWREARRRADLYPIAGHLPYTVNLGAAAERQ
QEFTRMVLHDDTLRVAAIDGEYLISHPGHYEGERQAGLDRIIQLIEEAYLSITPPGPMLLLETMAGQGKEVGTIDDLCYI
LEGLGWPDRVGVCLDSAHLFAAGWDLRTPAGCQQLVQELAAKIGLDRVKAMHLNDSAAPLGSHRDRHAGIGKGELGREGI
AAVVNDPFLGELPLFLETPVANYEEYGEEIALIQKLKSV

Specific function: Endonuclease IV plays a role in DNA repair. It cleaves phosphodiester bonds at apurinic or apyrimidinic sites (AP sites) to produce new 5'-ends that are base-free deoxyribose 5-phosphate residues. It preferentially attacks modified AP sites created by ble

COG id: COG0648

COG function: function code L; Endonuclease IV

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AP endonuclease 2 family

Homologues:

Organism=Escherichia coli, GI1788483, Length=280, Percent_Identity=33.9285714285714, Blast_Score=141, Evalue=5e-35,
Organism=Caenorhabditis elegans, GI17531193, Length=268, Percent_Identity=32.089552238806, Blast_Score=152, Evalue=2e-37,
Organism=Saccharomyces cerevisiae, GI6322735, Length=282, Percent_Identity=31.9148936170213, Blast_Score=144, Evalue=1e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): END4_MOOTA (Q2RJF1)

Other databases:

- EMBL:   CP000232
- RefSeq:   YP_429981.1
- ProteinModelPortal:   Q2RJF1
- SMR:   Q2RJF1
- STRING:   Q2RJF1
- GeneID:   3833257
- GenomeReviews:   CP000232_GR
- KEGG:   mta:Moth_1124
- NMPDR:   fig|264732.9.peg.1106
- eggNOG:   COG0648
- HOGENOM:   HBG565018
- OMA:   QIALETM
- PhylomeDB:   Q2RJF1
- BioCyc:   MTHE264732:MOTH_1124-MONOMER
- GO:   GO:0005622
- HAMAP:   MF_00152
- InterPro:   IPR018246
- InterPro:   IPR001719
- InterPro:   IPR013022
- InterPro:   IPR012307
- Gene3D:   G3DSA:3.20.20.150
- PANTHER:   PTHR21445
- SMART:   SM00518
- TIGRFAMs:   TIGR00587

Pfam domain/function: PF01261 AP_endonuc_2; SSF51658 Xyl_isomerase-like_TIM-brl

EC number: =3.1.21.2

Molecular weight: Translated: 30409; Mature: 30409

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: PS00729 AP_NUCLEASE_F2_1; PS00730 AP_NUCLEASE_F2_2; PS00731 AP_NUCLEASE_F2_3; PS51432 AP_NUCLEASE_F2_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLGAHLSIAKGLPRTAAMATSIGANTFQYFTRNPRGGAARQIPGKEIQAWREARRRADL
CCCCCCCHHHHCCCHHHHHHHHHCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCC
YPIAGHLPYTVNLGAAAERQQEFTRMVLHDDTLRVAAIDGEYLISHPGHYEGERQAGLDR
CEECCCCCEEEECCCHHHHHHHHHHHHHCCCCEEEEEECCCEEECCCCCCCCHHHHHHHH
IIQLIEEAYLSITPPGPMLLLETMAGQGKEVGTIDDLCYILEGLGWPDRVGVCLDSAHLF
HHHHHHHHHEECCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCHHHHHEECCCEEE
AAGWDLRTPAGCQQLVQELAAKIGLDRVKAMHLNDSAAPLGSHRDRHAGIGKGELGREGI
EECCCCCCCHHHHHHHHHHHHHHCHHHHHEEECCCCCCCCCCCCCHHCCCCCCCCCCCHH
AAVVNDPFLGELPLFLETPVANYEEYGEEIALIQKLKSV
HHHHCCCCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRLGAHLSIAKGLPRTAAMATSIGANTFQYFTRNPRGGAARQIPGKEIQAWREARRRADL
CCCCCCCHHHHCCCHHHHHHHHHCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCC
YPIAGHLPYTVNLGAAAERQQEFTRMVLHDDTLRVAAIDGEYLISHPGHYEGERQAGLDR
CEECCCCCEEEECCCHHHHHHHHHHHHHCCCCEEEEEECCCEEECCCCCCCCHHHHHHHH
IIQLIEEAYLSITPPGPMLLLETMAGQGKEVGTIDDLCYILEGLGWPDRVGVCLDSAHLF
HHHHHHHHHEECCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCHHHHHEECCCEEE
AAGWDLRTPAGCQQLVQELAAKIGLDRVKAMHLNDSAAPLGSHRDRHAGIGKGELGREGI
EECCCCCCCHHHHHHHHHHHHHHCHHHHHEEECCCCCCCCCCCCCHHCCCCCCCCCCCHH
AAVVNDPFLGELPLFLETPVANYEEYGEEIALIQKLKSV
HHHHCCCCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA