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The map label for this gene is ansB [C]

Identifier: 83595059

GI number: 83595059

Start: 4281965

End: 4282483

Strand: Direct

Name: ansB [C]

Synonym: Rru_A3730

Alternate gene names: 83595059

Gene position: 4281965-4282483 (Clockwise)

Preceding gene: 83595058

Following gene: 83595060

Centisome position: 98.37

GC content: 64.55

Gene sequence:

>519_bases
ATGGCCGTTTCCCCCTCGCCCCTGCGCATCTTCACCGCCGGCGGGACCATCGATAAAGACTATCGCCTGGAAGAAAACGG
GCTGGTCGTCGGCGACCCCTTCGTCGCCGAGGTCCTGAAAACGGCCCGTCTGGCGGGCGCGGTGTCGATTGTCGCGCTGT
CTCGCAAGGATAGCCTGGATTTCACCGAGGCCGACCGCGAAGCGATCGGCCGGGCGGTCGGCCAAGCCGTGGAGGATCAT
ATCCTTCTCACCCACGGCACCGACACCATGGTTGAAACCGCCCGCTATCTTGGCGGCTTGCCCGAACTCGCCGGAAAGAC
CGTGGTGTTGAGCGGCGCGATGGTCCCCGGCCGGGTGGGGGGAAGCGACGCGGCTTTCAATATCGGTTTCGCCTGCGCTG
CGGCGTTGATGCTGGCGCCGGGAGTCTATATTGCTATGCACGGCAAGGTTTTCGACCCGGCGAAAACCCGGAAGAATCGC
GGCCTTGGCCGGTTCGAGCCCATCGACGACCAGGAGTAG

Upstream 100 bases:

>100_bases
ACCAACTGGGCCTATAACCCGAAATAACAGTTACGGGGATTTTCGGCGGCGCCGGTTTGCTTGGCGTCGCCCCTTCCCTT
GCCACAGGATCCTCAAGGCC

Downstream 100 bases:

>100_bases
CTGGCGGAAAAGGGAACCTTGGGTGTCCTTGCCGCCGCCCCTTGTCTTCAAAGTCTCGACCGGATGGCACAGGAGGATCG
ATGGCGGAGCAGGCGGACGG

Product: asparaginase

Products: L-Aspartate; ammonia [C]

Alternate protein names: L-Asparaginase; Asparaginase/Glutaminase; Cytoplasmic L-Asparaginase I Protein; Glutamin-(Asparagin-)Ase Family Protein; Asparaginase Protein

Number of amino acids: Translated: 172; Mature: 171

Protein sequence:

>172_residues
MAVSPSPLRIFTAGGTIDKDYRLEENGLVVGDPFVAEVLKTARLAGAVSIVALSRKDSLDFTEADREAIGRAVGQAVEDH
ILLTHGTDTMVETARYLGGLPELAGKTVVLSGAMVPGRVGGSDAAFNIGFACAAALMLAPGVYIAMHGKVFDPAKTRKNR
GLGRFEPIDDQE

Sequences:

>Translated_172_residues
MAVSPSPLRIFTAGGTIDKDYRLEENGLVVGDPFVAEVLKTARLAGAVSIVALSRKDSLDFTEADREAIGRAVGQAVEDH
ILLTHGTDTMVETARYLGGLPELAGKTVVLSGAMVPGRVGGSDAAFNIGFACAAALMLAPGVYIAMHGKVFDPAKTRKNR
GLGRFEPIDDQE
>Mature_171_residues
AVSPSPLRIFTAGGTIDKDYRLEENGLVVGDPFVAEVLKTARLAGAVSIVALSRKDSLDFTEADREAIGRAVGQAVEDHI
LLTHGTDTMVETARYLGGLPELAGKTVVLSGAMVPGRVGGSDAAFNIGFACAAALMLAPGVYIAMHGKVFDPAKTRKNRG
LGRFEPIDDQE

Specific function: Unknown

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 3.5.1.1 [C]

Molecular weight: Translated: 18070; Mature: 17939

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVSPSPLRIFTAGGTIDKDYRLEENGLVVGDPFVAEVLKTARLAGAVSIVALSRKDSLD
CCCCCCCEEEEECCCCCCCCCEECCCCEEECCHHHHHHHHHHHHHHHEEEEEECCCCCCC
FTEADREAIGRAVGQAVEDHILLTHGTDTMVETARYLGGLPELAGKTVVLSGAMVPGRVG
CCHHHHHHHHHHHHHHHHCCEEEECCCHHHHHHHHHHCCCHHHCCCEEEEECCCCCCCCC
GSDAAFNIGFACAAALMLAPGVYIAMHGKVFDPAKTRKNRGLGRFEPIDDQE
CCCCHHHHHHHHHHHHHHCCCEEEEECCCEECCHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
AVSPSPLRIFTAGGTIDKDYRLEENGLVVGDPFVAEVLKTARLAGAVSIVALSRKDSLD
CCCCCCEEEEECCCCCCCCCEECCCCEEECCHHHHHHHHHHHHHHHEEEEEECCCCCCC
FTEADREAIGRAVGQAVEDHILLTHGTDTMVETARYLGGLPELAGKTVVLSGAMVPGRVG
CCHHHHHHHHHHHHHHHHCCEEEECCCHHHHHHHHHHCCCHHHCCCEEEEECCCCCCCCC
GSDAAFNIGFACAAALMLAPGVYIAMHGKVFDPAKTRKNRGLGRFEPIDDQE
CCCCHHHHHHHHHHHHHHCCCEEEEECCCEECCHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-Asparagine; H2O [C]

Specific reaction: L-Asparagine + H2O --> L-Aspartate + ammonia [C]

General reaction: Carboxylic acid amide hydrolysis [C]

Inhibitor: 5-Bromo-4-oxo-L-norvaline; Hg2+; NH3 [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA