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The map label for this gene is gsp [H]

Identifier: 83595052

GI number: 83595052

Start: 4274023

End: 4275951

Strand: Direct

Name: gsp [H]

Synonym: Rru_A3723

Alternate gene names: 83595052

Gene position: 4274023-4275951 (Clockwise)

Preceding gene: 83595051

Following gene: 83595053

Centisome position: 98.19

GC content: 64.13

Gene sequence:

>1929_bases
ATGAGCCACCATCATACCGCCGAGTCCGCCCCCTTTGGCACCGTCCTGGGGTATGGCCCCGGCGGGGTCGCCGTCTATTC
GTCCGATTACGATACCGTCGACCGGCGGGCTTTGCCCGATCGCGCCAGCTTTCGCAGCGTCATCGATGGCCAGTACATGG
GCCATAAATGGCAATGCGTCGAACTGGCGCGGCGCTTTTTGTATCTGACCCACGGCTATGTCTTTGACGATATCGCCATG
GCCTATGACATCTTCCGCCTGCGCACGGTCCGCGTCGTCGCCGAGGACCGCCACCTGCCGCTGCGCTCCTTTCGCAACGG
CGCGCGCCGCTGGCCCGAGGCCGGGTCCCTGCTGATCTGGGACGAGGGCGGCGAGTTCGAGGTGACCGGCCATGTCGCCG
TGGTCACCGAGGTCTTTGCCGACCGCCTGCGCTGCATTGAACAAAACGTCGATGACCGCGTCTGGCCGACAGGTCAGAGC
TATTCGCGGGAACTGCCCGCCCGCGTCGATGACCAGGGCGGCTATCATGTGGCGTGCACCTTTGCCGACACGGCTATTTT
GGGTTGGGTGATGCAGACCGCCGATCCCCTGTTCTCCGAAGAGACCACCGAGGCCGACCGCCAGTTGTTCGTGCCCCAAC
GCCGCACCGTCGCCGCCAAGCCCGGAGGCGCGCCCGCCCTGTGGCTCGACCCGGCCAAACCCGACGAGGCGGCCTATATG
GCGATGATGGGTGGCTGCGCCCTGACCAATCGCCCGGCCGACCGCGAAACCTATTTCCGCATCAGCCAGACCGCCGAACG
GGAATTGAAGCGCGCCACCAACGAATTGCATGCCATGTTCCTCTACGCCACCGATCATGTGCTGCAAAACGACGCCCTGC
TCGCCCAGTTCATGCTGCCCCAGGCCCTGTGGCCGCGCATCCGCCAGTCCTGGGCCAATCGGCGCGGCCGCATGATCACC
GGTCGCTTCGACTTCTCGATGTCGGAGCGCGGGCTGAAAGTTTACGAATACAACGCCGATTCCGCCTCGTGCCATATGGA
ATGCGGCAAGGTGCAACAGGCCTGGGCGGCCTATTACGGCTGTCAGGAGGGGCGCTGTTCGGGCGAGGATGTGCATGGCG
CCCTGGTCGCGGCCTGGCGGGAAAGCGGGGTCGACAGCGTTTTGCACATCATGCTCGACCATGACCAGGAAGAGCTGTAC
CACGCCCTCTATATGAAAACGGCGATCGAGGAGGCGGGGATCGCCTGCAAGATCATCACCGGTGTCGATGGCCTGTTGTG
GGACGACGAGGGCTTTGTCACCGATGCCGATGGGGTGCGCATCACCTGGGTGTGGAAGACCTGGGCCTGGGAAACGGCGA
TCGATGAATTGCGCGCCCAATGCGCCGCCGACGACGAGGAAATCCGCTTGCATCGGCCGGTCGACCATTCGCGCCATCCG
CCGCGGCTGATCGATGTGCTGCTGCGCAAGGAGGTGATGGTCTTCGAGCCGCTGTGGACGCTGATCCCCAGCAACAAGGC
GATCTTGCCCGTGCTGTGGATGATGTTTCCCAACCATCCTTATTTGCTCGACAGCCAGTTCAGCGTGACCAATGCCTTGC
GCGAAAGCGGCTATGTCGCCAAGCCGATCGTCGGGCGCTGCGGATCGAACATCAGCATTTTCGATAAGACCAGCGACCTG
ATCGGCGAAACCGCCGGCCAGTTCGGCAATCGCACCATGATCTATCAGGAACTGTGCCCCTTGCCGCGAATCGGCGGCGA
CAACGTGCAGATCGGCACCTTCTCGGTGCGCGGCCGCTATGCCGGCTCCAGCGTCCGCGTCGATCCGGCGGCGATCATCA
CCACCCACAGCGATCTTCTGGCGCTGCGCATCGTCGAAGACGCGCTGCTCGCCGAGGACGCCGACCCGCGGGCCCCGTCC
TCCCCGTAA

Upstream 100 bases:

>100_bases
GCGATCCGTGGAATTTTCCCGATAACACCGGCGACCGATCGACCCCATATGGGGATCGTGCCGCTTCCTGGTCCTTTCCC
GTGACGAGAACCCTTTCCCC

Downstream 100 bases:

>100_bases
TCGCCGGCGCTTTCCCGGCGGACGGAAGGCGAAGCCCGCCATTTGGCCCTGGTCGCCGCCGGGAGGGGGACCGGCGACCG
GGGGCATCACACCCGAGCCG

Product: bifunctional glutathionylspermidine amidase/glutathionylspermidine synthetase

Products: NA

Alternate protein names: Glutathionylspermidine synthase; GSP synthetase; Glutathione:spermidine ligase [ADP-forming]; Glutathionylspermidine amidase; Glutathionylspermidine amidohydrolase [spermidine-forming]; GSP amidase [H]

Number of amino acids: Translated: 642; Mature: 641

Protein sequence:

>642_residues
MSHHHTAESAPFGTVLGYGPGGVAVYSSDYDTVDRRALPDRASFRSVIDGQYMGHKWQCVELARRFLYLTHGYVFDDIAM
AYDIFRLRTVRVVAEDRHLPLRSFRNGARRWPEAGSLLIWDEGGEFEVTGHVAVVTEVFADRLRCIEQNVDDRVWPTGQS
YSRELPARVDDQGGYHVACTFADTAILGWVMQTADPLFSEETTEADRQLFVPQRRTVAAKPGGAPALWLDPAKPDEAAYM
AMMGGCALTNRPADRETYFRISQTAERELKRATNELHAMFLYATDHVLQNDALLAQFMLPQALWPRIRQSWANRRGRMIT
GRFDFSMSERGLKVYEYNADSASCHMECGKVQQAWAAYYGCQEGRCSGEDVHGALVAAWRESGVDSVLHIMLDHDQEELY
HALYMKTAIEEAGIACKIITGVDGLLWDDEGFVTDADGVRITWVWKTWAWETAIDELRAQCAADDEEIRLHRPVDHSRHP
PRLIDVLLRKEVMVFEPLWTLIPSNKAILPVLWMMFPNHPYLLDSQFSVTNALRESGYVAKPIVGRCGSNISIFDKTSDL
IGETAGQFGNRTMIYQELCPLPRIGGDNVQIGTFSVRGRYAGSSVRVDPAAIITTHSDLLALRIVEDALLAEDADPRAPS
SP

Sequences:

>Translated_642_residues
MSHHHTAESAPFGTVLGYGPGGVAVYSSDYDTVDRRALPDRASFRSVIDGQYMGHKWQCVELARRFLYLTHGYVFDDIAM
AYDIFRLRTVRVVAEDRHLPLRSFRNGARRWPEAGSLLIWDEGGEFEVTGHVAVVTEVFADRLRCIEQNVDDRVWPTGQS
YSRELPARVDDQGGYHVACTFADTAILGWVMQTADPLFSEETTEADRQLFVPQRRTVAAKPGGAPALWLDPAKPDEAAYM
AMMGGCALTNRPADRETYFRISQTAERELKRATNELHAMFLYATDHVLQNDALLAQFMLPQALWPRIRQSWANRRGRMIT
GRFDFSMSERGLKVYEYNADSASCHMECGKVQQAWAAYYGCQEGRCSGEDVHGALVAAWRESGVDSVLHIMLDHDQEELY
HALYMKTAIEEAGIACKIITGVDGLLWDDEGFVTDADGVRITWVWKTWAWETAIDELRAQCAADDEEIRLHRPVDHSRHP
PRLIDVLLRKEVMVFEPLWTLIPSNKAILPVLWMMFPNHPYLLDSQFSVTNALRESGYVAKPIVGRCGSNISIFDKTSDL
IGETAGQFGNRTMIYQELCPLPRIGGDNVQIGTFSVRGRYAGSSVRVDPAAIITTHSDLLALRIVEDALLAEDADPRAPS
SP
>Mature_641_residues
SHHHTAESAPFGTVLGYGPGGVAVYSSDYDTVDRRALPDRASFRSVIDGQYMGHKWQCVELARRFLYLTHGYVFDDIAMA
YDIFRLRTVRVVAEDRHLPLRSFRNGARRWPEAGSLLIWDEGGEFEVTGHVAVVTEVFADRLRCIEQNVDDRVWPTGQSY
SRELPARVDDQGGYHVACTFADTAILGWVMQTADPLFSEETTEADRQLFVPQRRTVAAKPGGAPALWLDPAKPDEAAYMA
MMGGCALTNRPADRETYFRISQTAERELKRATNELHAMFLYATDHVLQNDALLAQFMLPQALWPRIRQSWANRRGRMITG
RFDFSMSERGLKVYEYNADSASCHMECGKVQQAWAAYYGCQEGRCSGEDVHGALVAAWRESGVDSVLHIMLDHDQEELYH
ALYMKTAIEEAGIACKIITGVDGLLWDDEGFVTDADGVRITWVWKTWAWETAIDELRAQCAADDEEIRLHRPVDHSRHPP
RLIDVLLRKEVMVFEPLWTLIPSNKAILPVLWMMFPNHPYLLDSQFSVTNALRESGYVAKPIVGRCGSNISIFDKTSDLI
GETAGQFGNRTMIYQELCPLPRIGGDNVQIGTFSVRGRYAGSSVRVDPAAIITTHSDLLALRIVEDALLAEDADPRAPSS
P

Specific function: Catalyzes the formation of an amide bond between glutathione and spermidine coupled with hydrolysis of ATP; also catalyzes the hydrolysis of glutathionylspermidine to glutathione and spermidine [H]

COG id: COG0754

COG function: function code E; Glutathionylspermidine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 peptidase C51 domain [H]

Homologues:

Organism=Escherichia coli, GI1789361, Length=626, Percent_Identity=48.2428115015974, Blast_Score=590, Evalue=1e-169,
Organism=Escherichia coli, GI1789416, Length=381, Percent_Identity=28.0839895013123, Blast_Score=137, Evalue=2e-33,
Organism=Escherichia coli, GI1790629, Length=365, Percent_Identity=28.4931506849315, Blast_Score=129, Evalue=8e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007921
- InterPro:   IPR005494
- InterPro:   IPR016185 [H]

Pfam domain/function: PF05257 CHAP; PF03738 GSP_synth [H]

EC number: =6.3.1.8; =3.5.1.78 [H]

Molecular weight: Translated: 72141; Mature: 72010

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: PS50911 CHAP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHHHTAESAPFGTVLGYGPGGVAVYSSDYDTVDRRALPDRASFRSVIDGQYMGHKWQCV
CCCCCCCCCCCCCCEEEECCCCEEEEECCCCCHHHHCCCCHHHHHHHHCCCCCCCCHHHH
ELARRFLYLTHGYVFDDIAMAYDIFRLRTVRVVAEDRHLPLRSFRNGARRWPEAGSLLIW
HHHHHHHHHHCCHHHHHHHHHHHHHHHHEEEEEECCCCCCHHHHHHHHHCCCCCCCEEEE
DEGGEFEVTGHVAVVTEVFADRLRCIEQNVDDRVWPTGQSYSRELPARVDDQGGYHVACT
CCCCCEEEECHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEE
FADTAILGWVMQTADPLFSEETTEADRQLFVPQRRTVAAKPGGAPALWLDPAKPDEAAYM
EHHHHHHHHHHHHCCCCCCCCCCCCHHHEECCCCCEEEECCCCCCEEEECCCCCCHHHHH
AMMGGCALTNRPADRETYFRISQTAERELKRATNELHAMFLYATDHVLQNDALLAQFMLP
HHHCCCEECCCCCCCHHHEEHHHHHHHHHHHHHHHHHEEEEEEHHHHHHCHHHHHHHHHH
QALWPRIRQSWANRRGRMITGRFDFSMSERGLKVYEYNADSASCHMECGKVQQAWAAYYG
HHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHC
CQEGRCSGEDVHGALVAAWRESGVDSVLHIMLDHDQEELYHALYMKTAIEEAGIACKIIT
CCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCEEEEEE
GVDGLLWDDEGFVTDADGVRITWVWKTWAWETAIDELRAQCAADDEEIRLHRPVDHSRHP
CCCCEEECCCCCEECCCCEEEEEEEECCCHHHHHHHHHHHHCCCCCCEEEECCCCCCCCC
PRLIDVLLRKEVMVFEPLWTLIPSNKAILPVLWMMFPNHPYLLDSQFSVTNALRESGYVA
HHHHHHHHHHHHHHCCHHHHHCCCCCCHHHHHHHHCCCCCEEECCCHHHHHHHHHCCCCC
KPIVGRCGSNISIFDKTSDLIGETAGQFGNRTMIYQELCPLPRIGGDNVQIGTFSVRGRY
CCHHHHCCCCCEEEECCHHHHHHHHHHCCCCEEEHHHHCCCCCCCCCCEEEEEEEEECEE
AGSSVRVDPAAIITTHSDLLALRIVEDALLAEDADPRAPSSP
CCCCEEECCEEEEEECCHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
SHHHTAESAPFGTVLGYGPGGVAVYSSDYDTVDRRALPDRASFRSVIDGQYMGHKWQCV
CCCCCCCCCCCCCEEEECCCCEEEEECCCCCHHHHCCCCHHHHHHHHCCCCCCCCHHHH
ELARRFLYLTHGYVFDDIAMAYDIFRLRTVRVVAEDRHLPLRSFRNGARRWPEAGSLLIW
HHHHHHHHHHCCHHHHHHHHHHHHHHHHEEEEEECCCCCCHHHHHHHHHCCCCCCCEEEE
DEGGEFEVTGHVAVVTEVFADRLRCIEQNVDDRVWPTGQSYSRELPARVDDQGGYHVACT
CCCCCEEEECHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEE
FADTAILGWVMQTADPLFSEETTEADRQLFVPQRRTVAAKPGGAPALWLDPAKPDEAAYM
EHHHHHHHHHHHHCCCCCCCCCCCCHHHEECCCCCEEEECCCCCCEEEECCCCCCHHHHH
AMMGGCALTNRPADRETYFRISQTAERELKRATNELHAMFLYATDHVLQNDALLAQFMLP
HHHCCCEECCCCCCCHHHEEHHHHHHHHHHHHHHHHHEEEEEEHHHHHHCHHHHHHHHHH
QALWPRIRQSWANRRGRMITGRFDFSMSERGLKVYEYNADSASCHMECGKVQQAWAAYYG
HHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHC
CQEGRCSGEDVHGALVAAWRESGVDSVLHIMLDHDQEELYHALYMKTAIEEAGIACKIIT
CCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCEEEEEE
GVDGLLWDDEGFVTDADGVRITWVWKTWAWETAIDELRAQCAADDEEIRLHRPVDHSRHP
CCCCEEECCCCCEECCCCEEEEEEEECCCHHHHHHHHHHHHCCCCCCEEEECCCCCCCCC
PRLIDVLLRKEVMVFEPLWTLIPSNKAILPVLWMMFPNHPYLLDSQFSVTNALRESGYVA
HHHHHHHHHHHHHHCCHHHHHCCCCCCHHHHHHHHCCCCCEEECCCHHHHHHHHHCCCCC
KPIVGRCGSNISIFDKTSDLIGETAGQFGNRTMIYQELCPLPRIGGDNVQIGTFSVRGRY
CCHHHHCCCCCEEEECCHHHHHHHHHHCCCCEEEHHHHCCCCCCCCCCEEEEEEEEECEE
AGSSVRVDPAAIITTHSDLLALRIVEDALLAEDADPRAPSSP
CCCCEEECCEEEEEECCHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7775463; 9278503 [H]