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The map label for this gene is wcaJ [H]

Identifier: 83595050

GI number: 83595050

Start: 4271848

End: 4272936

Strand: Direct

Name: wcaJ [H]

Synonym: Rru_A3721

Alternate gene names: 83595050

Gene position: 4271848-4272936 (Clockwise)

Preceding gene: 83595049

Following gene: 83595051

Centisome position: 98.14

GC content: 64.92

Gene sequence:

>1089_bases
ATGGCTTCCGCAGAGACATCCTTGCACGCCTTGGCCGAAGACAAGCGCGGCACGAGGCGCCCGTCCTCGCCGTCCCGCCT
TCCTAGGCCGATGCGCGTCGCCCTGGTCGGCGCCGGTCTTCCCGCCGCCGCCCTGATCGAATTCCTCCAATCCGACGACG
CCCGCGACGATTACACCGTCGTGGCGATCTTCGATGAACGCGGCGATCGCCGGCCACCGGTGTTGGGTGCCAAACCGGTG
GAAAAGGGCCTGAGCGGCCTGCGCGACCTTGCCGAGGCCGGCAAGATCGACGCCATTCTTCTGACGCTCTACGGCGCCTC
CTCGGCGCGCATGTTCGAGATCATCGAGCGCATCGGCACCACCGCCGTCGATATCTATCTGCCGCGCGAGCACAAAGATA
AGCACTTCTCGTGGACCAGCTATCACCTGATCGGCGGCCTGCCGTTCCTGTGCATCAAGGGGCGGCCCTTCCAGGGGTTC
GCCGGCGTTTTCAAAAGGATCGAGGATTATACCCTGGCGGTTCTGGCGCTGACGCTGATCGGCCCGATCCTGCTGCTGGC
CATGCTGGCGATCCGCCTGGAAAGCCCCGGCCCGGCCCTGATCCATCAGCGGCGCATCGGCCTTGGCGGGAAGCTGTTTT
CCATGCTCAAGTTGCGCTCCATGCGCTTCGATCCCAATGACGACGGGCGGAACGGCGCCATCGCCAATGATCCGCGCATC
ACCCGGGTCGGCGCCTTCCTGCGCGCCACCAGCATCGACGAGCTGCCCCAGGTCCTCAATGTCCTGCGCGGCGACATGTC
GATGATCGGCCCGCGTCCCCATGTTCCCAACATGCTGGTCGAAAACACCGTTTACGGGGTGAGTGTCAGCGAATACGTCG
CCCGCCACCGGGTGCGGCCGGGGATCACCGGTTGGGCCCAGGTGAATGGCATGCGCGGCGGCATTCATGATATCGAAAAG
GCCCGGCGCGGCGCGCAACTGGATATCTATTACATCGAGAACTGGACACCATGGCTCGACATCAAGATCCTCTGGCGGAC
GATTTTCGGCGGCCTGCGCGACCCCTCCGCTCTGCGCTCGCCGGGCTGA

Upstream 100 bases:

>100_bases
ATGGAGCCTGCCGCTGCCGGCCGGCCTCGCGCGCGTGCTGTTTGGTTCACCCCCCACCGTGTCTTCCGTTTCCCCGACGA
ATACGAGGTGAATGATCGGC

Downstream 100 bases:

>100_bases
TCGCCGCCGCCGTTTTGGCGTTTCCCGCCTTGCCGGCCCGGGCTGACATCCCCCAACGCCCGCTTGTCGCCTATGTGCAA
AGCTGGGAAGACGGCCCGGC

Product: sugar transferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 362; Mature: 361

Protein sequence:

>362_residues
MASAETSLHALAEDKRGTRRPSSPSRLPRPMRVALVGAGLPAAALIEFLQSDDARDDYTVVAIFDERGDRRPPVLGAKPV
EKGLSGLRDLAEAGKIDAILLTLYGASSARMFEIIERIGTTAVDIYLPREHKDKHFSWTSYHLIGGLPFLCIKGRPFQGF
AGVFKRIEDYTLAVLALTLIGPILLLAMLAIRLESPGPALIHQRRIGLGGKLFSMLKLRSMRFDPNDDGRNGAIANDPRI
TRVGAFLRATSIDELPQVLNVLRGDMSMIGPRPHVPNMLVENTVYGVSVSEYVARHRVRPGITGWAQVNGMRGGIHDIEK
ARRGAQLDIYYIENWTPWLDIKILWRTIFGGLRDPSALRSPG

Sequences:

>Translated_362_residues
MASAETSLHALAEDKRGTRRPSSPSRLPRPMRVALVGAGLPAAALIEFLQSDDARDDYTVVAIFDERGDRRPPVLGAKPV
EKGLSGLRDLAEAGKIDAILLTLYGASSARMFEIIERIGTTAVDIYLPREHKDKHFSWTSYHLIGGLPFLCIKGRPFQGF
AGVFKRIEDYTLAVLALTLIGPILLLAMLAIRLESPGPALIHQRRIGLGGKLFSMLKLRSMRFDPNDDGRNGAIANDPRI
TRVGAFLRATSIDELPQVLNVLRGDMSMIGPRPHVPNMLVENTVYGVSVSEYVARHRVRPGITGWAQVNGMRGGIHDIEK
ARRGAQLDIYYIENWTPWLDIKILWRTIFGGLRDPSALRSPG
>Mature_361_residues
ASAETSLHALAEDKRGTRRPSSPSRLPRPMRVALVGAGLPAAALIEFLQSDDARDDYTVVAIFDERGDRRPPVLGAKPVE
KGLSGLRDLAEAGKIDAILLTLYGASSARMFEIIERIGTTAVDIYLPREHKDKHFSWTSYHLIGGLPFLCIKGRPFQGFA
GVFKRIEDYTLAVLALTLIGPILLLAMLAIRLESPGPALIHQRRIGLGGKLFSMLKLRSMRFDPNDDGRNGAIANDPRIT
RVGAFLRATSIDELPQVLNVLRGDMSMIGPRPHVPNMLVENTVYGVSVSEYVARHRVRPGITGWAQVNGMRGGIHDIEKA
RRGAQLDIYYIENWTPWLDIKILWRTIFGGLRDPSALRSPG

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG2148

COG function: function code M; Sugar transferases involved in lipopolysaccharide synthesis

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial sugar transferase family [H]

Homologues:

Organism=Escherichia coli, GI1788360, Length=275, Percent_Identity=38.9090909090909, Blast_Score=208, Evalue=4e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003362
- InterPro:   IPR017475
- InterPro:   IPR017473 [H]

Pfam domain/function: PF02397 Bac_transf [H]

EC number: NA

Molecular weight: Translated: 39972; Mature: 39841

Theoretical pI: Translated: 10.24; Mature: 10.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASAETSLHALAEDKRGTRRPSSPSRLPRPMRVALVGAGLPAAALIEFLQSDDARDDYTV
CCCCHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHCCCCCCCEEE
VAIFDERGDRRPPVLGAKPVEKGLSGLRDLAEAGKIDAILLTLYGASSARMFEIIERIGT
EEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCHHHHHHHHHHCC
TAVDIYLPREHKDKHFSWTSYHLIGGLPFLCIKGRPFQGFAGVFKRIEDYTLAVLALTLI
EEEEEEECCCCCCCCCCEEEEHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHH
GPILLLAMLAIRLESPGPALIHQRRIGLGGKLFSMLKLRSMRFDPNDDGRNGAIANDPRI
HHHHHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCH
TRVGAFLRATSIDELPQVLNVLRGDMSMIGPRPHVPNMLVENTVYGVSVSEYVARHRVRP
HHHHHHHHHCCHHHHHHHHHHHHCCHHHCCCCCCCCCHHHHCCEECCCHHHHHHHHCCCC
GITGWAQVNGMRGGIHDIEKARRGAQLDIYYIENWTPWLDIKILWRTIFGGLRDPSALRS
CCCCHHEECCCCCCHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCCCHHCCC
PG
CC
>Mature Secondary Structure 
ASAETSLHALAEDKRGTRRPSSPSRLPRPMRVALVGAGLPAAALIEFLQSDDARDDYTV
CCCHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHCCCCCCCEEE
VAIFDERGDRRPPVLGAKPVEKGLSGLRDLAEAGKIDAILLTLYGASSARMFEIIERIGT
EEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCHHHHHHHHHHCC
TAVDIYLPREHKDKHFSWTSYHLIGGLPFLCIKGRPFQGFAGVFKRIEDYTLAVLALTLI
EEEEEEECCCCCCCCCCEEEEHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHH
GPILLLAMLAIRLESPGPALIHQRRIGLGGKLFSMLKLRSMRFDPNDDGRNGAIANDPRI
HHHHHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCH
TRVGAFLRATSIDELPQVLNVLRGDMSMIGPRPHVPNMLVENTVYGVSVSEYVARHRVRP
HHHHHHHHHCCHHHHHHHHHHHHCCHHHCCCCCCCCCHHHHCCEECCCHHHHHHHHCCCC
GITGWAQVNGMRGGIHDIEKARRGAQLDIYYIENWTPWLDIKILWRTIFGGLRDPSALRS
CCCCHHEECCCCCCHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCCCHHCCC
PG
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8759852; 9097040; 9278503 [H]