Click here to switch to the map view.

The map label for this gene is dapL [H]

Identifier: 83595035

GI number: 83595035

Start: 4255970

End: 4257352

Strand: Reverse

Name: dapL [H]

Synonym: Rru_A3706

Alternate gene names: 83595035

Gene position: 4257352-4255970 (Counterclockwise)

Preceding gene: 83595037

Following gene: 83595034

Centisome position: 97.81

GC content: 70.57

Gene sequence:

>1383_bases
ATGGCGTTCGCCGCCGGATTTGTCCAGGGAGTCCGCCCTTTTTGGCCAAAGGGATCGCCCACCGATGGCACCGGAGCTTG
CCCCTTTCCGGGTGGCCGCGTACTCTTTCGGCCACACCCCCCGGGGAGGGCCCGTCCCTTGTTCGGGGTCTCGCGCCCCC
GATCCGTAGCGGACCACCCCATGATCAATCCCCGGCTCATCGCCCTGCCCGATTATCCGTTCCAGCGTCTGGCCACCTTG
CTGGCCGGGGTCGCCCCCGGAGCCGATCCCCTGGCGCTGTCGGTGGGCGAGCCCCAACACCCGACCCCGGCGCTGGCCGG
CCTGGAACTGGCCCGTCACGGCGACCAATGGCGGCGCTATCCGCCCGCCGCCGGCAATGAGCGCTGGAAGGCGGCGGTCA
CCGCTTGGCTGACCCGGCGCTATGGCCTGCCCGCCGCGATGATCGATCCGCACGCGGTGATCCCGGCCTCGGGCACCCGC
GAGGCGTTGTACATGCTGGGCCAGGTGCTGACCGATGGCCGGGGCGAGGCTGGCGAGCGGCCGCTCGTGGTCATGCCCGA
TCCGCTTTATCTGCCCTATCTGGGCGCGGCCAGGATGAACGGCGCGACCATTCTGGCCGTTCCCGGGTCGGAAGAGGGGG
ATTTCCTGCCCGATATCGCCGGCCTGCCCGCCGCCACCCTGGAGCGCGCCGCCGCCGTCTTCGTTTGCTCGCCGGCCAAT
CCGCAAGGCTCGATCGTCAGCCTGGAGTCGTGGAAAACCCTGGTCGGGCTGGCGCGCCGCCACGGCTTCGTGCTGGTCGC
CGATGAATGCTATAGCGAGATCTACGACGACCAGCCGCCGCCCGGCGTCCTTGAGGCCTGCCGCGACCTTGGCGGGTCGC
TGAAGAACGTGCTGGTGCTTAACAGCCTGTCGAAGCGCTCCAATGGCGCCGGGCTGCGCTCGGGGTTGATCGCCGGCGAT
CGGGCGCTGATCGACGCCTTCTTGCGCCTGCGCGCCTACGCCTCGGCGGGGATGCCCTTGCCGATCATGGAGGCCAGCGC
CGCCCTGTGGGACGACGAGACCCATGTCGAGGCCAACCGCGCCCTCTATCGCGAGAAGATCGATCTGGCCGCGGCGCGGC
TGAAGGGCATGCTGGGGTTTCGCCGGCCGCCCGGCGGGTTCTTCCTGTGGCTGGACACCACGCCGTGGGGCGAGACCGGC
GAGCAGGCGACGCTGCGGCTGTGGCGTGACCATGGACTGAAGGTGCTGCCCGGCGCCTATATGACCGCCAATCCCGATGC
CGGCGACAATCCCGCCCGCAACCGGCTGCGGCTGGCCCTGGTCCATGACGGCCCGGTGGTCGCCCGGGCCCTGGACCGGC
TGGTCGCGGCAAGCCCCCACTGA

Upstream 100 bases:

>100_bases
ACAGGGGTTCGCTCACACCGTACTCCATCGAAATCGGGGGATCGGCCGGGGGAAAGCGGGGCCGGACAGGCGCGAAGCGC
CGGGAAACGATACTTCAGAC

Downstream 100 bases:

>100_bases
TCCCAACCCTTCGCGGAGCCTTCATGGCCGATCGTACCGCCCGTTCGCGACTGCTGCCCCCGGCCGCCATCATCGCCCTT
CGCTCGGCCCTGCTGCGGGT

Product: aminotransferase

Products: NA

Alternate protein names: DAP-AT; DAP-aminotransferase; LL-DAP-aminotransferase [H]

Number of amino acids: Translated: 460; Mature: 459

Protein sequence:

>460_residues
MAFAAGFVQGVRPFWPKGSPTDGTGACPFPGGRVLFRPHPPGRARPLFGVSRPRSVADHPMINPRLIALPDYPFQRLATL
LAGVAPGADPLALSVGEPQHPTPALAGLELARHGDQWRRYPPAAGNERWKAAVTAWLTRRYGLPAAMIDPHAVIPASGTR
EALYMLGQVLTDGRGEAGERPLVVMPDPLYLPYLGAARMNGATILAVPGSEEGDFLPDIAGLPAATLERAAAVFVCSPAN
PQGSIVSLESWKTLVGLARRHGFVLVADECYSEIYDDQPPPGVLEACRDLGGSLKNVLVLNSLSKRSNGAGLRSGLIAGD
RALIDAFLRLRAYASAGMPLPIMEASAALWDDETHVEANRALYREKIDLAAARLKGMLGFRRPPGGFFLWLDTTPWGETG
EQATLRLWRDHGLKVLPGAYMTANPDAGDNPARNRLRLALVHDGPVVARALDRLVAASPH

Sequences:

>Translated_460_residues
MAFAAGFVQGVRPFWPKGSPTDGTGACPFPGGRVLFRPHPPGRARPLFGVSRPRSVADHPMINPRLIALPDYPFQRLATL
LAGVAPGADPLALSVGEPQHPTPALAGLELARHGDQWRRYPPAAGNERWKAAVTAWLTRRYGLPAAMIDPHAVIPASGTR
EALYMLGQVLTDGRGEAGERPLVVMPDPLYLPYLGAARMNGATILAVPGSEEGDFLPDIAGLPAATLERAAAVFVCSPAN
PQGSIVSLESWKTLVGLARRHGFVLVADECYSEIYDDQPPPGVLEACRDLGGSLKNVLVLNSLSKRSNGAGLRSGLIAGD
RALIDAFLRLRAYASAGMPLPIMEASAALWDDETHVEANRALYREKIDLAAARLKGMLGFRRPPGGFFLWLDTTPWGETG
EQATLRLWRDHGLKVLPGAYMTANPDAGDNPARNRLRLALVHDGPVVARALDRLVAASPH
>Mature_459_residues
AFAAGFVQGVRPFWPKGSPTDGTGACPFPGGRVLFRPHPPGRARPLFGVSRPRSVADHPMINPRLIALPDYPFQRLATLL
AGVAPGADPLALSVGEPQHPTPALAGLELARHGDQWRRYPPAAGNERWKAAVTAWLTRRYGLPAAMIDPHAVIPASGTRE
ALYMLGQVLTDGRGEAGERPLVVMPDPLYLPYLGAARMNGATILAVPGSEEGDFLPDIAGLPAATLERAAAVFVCSPANP
QGSIVSLESWKTLVGLARRHGFVLVADECYSEIYDDQPPPGVLEACRDLGGSLKNVLVLNSLSKRSNGAGLRSGLIAGDR
ALIDAFLRLRAYASAGMPLPIMEASAALWDDETHVEANRALYREKIDLAAARLKGMLGFRRPPGGFFLWLDTTPWGETGE
QATLRLWRDHGLKVLPGAYMTANPDAGDNPARNRLRLALVHDGPVVARALDRLVAASPH

Specific function: Involved in the synthesis of meso-diaminopimelate (m-DAP or DL-DAP), required for both lysine and peptidoglycan biosynthesis. Catalyzes the direct conversion of tetrahydrodipicolinate to LL-diaminopimelate, a reaction that requires three enzymes in E.coli

COG id: COG0436

COG function: function code E; Aspartate/tyrosine/aromatic aminotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. LL-diaminopimelate aminotransferase subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788722, Length=396, Percent_Identity=25.7575757575758, Blast_Score=99, Evalue=7e-22,
Organism=Escherichia coli, GI1790797, Length=219, Percent_Identity=25.1141552511416, Blast_Score=63, Evalue=4e-11,
Organism=Escherichia coli, GI1788627, Length=160, Percent_Identity=26.875, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004839
- InterPro:   IPR019881
- InterPro:   IPR004838
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00155 Aminotran_1_2 [H]

EC number: =2.6.1.83 [H]

Molecular weight: Translated: 49255; Mature: 49124

Theoretical pI: Translated: 8.84; Mature: 8.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFAAGFVQGVRPFWPKGSPTDGTGACPFPGGRVLFRPHPPGRARPLFGVSRPRSVADHP
CCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCC
MINPRLIALPDYPFQRLATLLAGVAPGADPLALSVGEPQHPTPALAGLELARHGDQWRRY
CCCCEEEEECCCCHHHHHHHHHHCCCCCCCEEEECCCCCCCCHHHHHHHHHHCCCHHHCC
PPAAGNERWKAAVTAWLTRRYGLPAAMIDPHAVIPASGTREALYMLGQVLTDGRGEAGER
CCCCCCCHHHHHHHHHHHHHCCCCCHHCCCCEECCCCCCHHHHHHHHHHHHCCCCCCCCC
PLVVMPDPLYLPYLGAARMNGATILAVPGSEEGDFLPDIAGLPAATLERAAAVFVCSPAN
CEEEECCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHCCEEEEEECCCC
PQGSIVSLESWKTLVGLARRHGFVLVADECYSEIYDDQPPPGVLEACRDLGGSLKNVLVL
CCCCEEEHHHHHHHHHHHHHCCEEEEHHHHHHHHHCCCCCCHHHHHHHHHCHHHHHHHHH
NSLSKRSNGAGLRSGLIAGDRALIDAFLRLRAYASAGMPLPIMEASAALWDDETHVEANR
HHHHCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCHHHHHH
ALYREKIDLAAARLKGMLGFRRPPGGFFLWLDTTPWGETGEQATLRLWRDHGLKVLPGAY
HHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHCCCEEECCCE
MTANPDAGDNPARNRLRLALVHDGPVVARALDRLVAASPH
EECCCCCCCCCCCCCEEEEEEECCHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
AFAAGFVQGVRPFWPKGSPTDGTGACPFPGGRVLFRPHPPGRARPLFGVSRPRSVADHP
CCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCC
MINPRLIALPDYPFQRLATLLAGVAPGADPLALSVGEPQHPTPALAGLELARHGDQWRRY
CCCCEEEEECCCCHHHHHHHHHHCCCCCCCEEEECCCCCCCCHHHHHHHHHHCCCHHHCC
PPAAGNERWKAAVTAWLTRRYGLPAAMIDPHAVIPASGTREALYMLGQVLTDGRGEAGER
CCCCCCCHHHHHHHHHHHHHCCCCCHHCCCCEECCCCCCHHHHHHHHHHHHCCCCCCCCC
PLVVMPDPLYLPYLGAARMNGATILAVPGSEEGDFLPDIAGLPAATLERAAAVFVCSPAN
CEEEECCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHCCEEEEEECCCC
PQGSIVSLESWKTLVGLARRHGFVLVADECYSEIYDDQPPPGVLEACRDLGGSLKNVLVL
CCCCEEEHHHHHHHHHHHHHCCEEEEHHHHHHHHHCCCCCCHHHHHHHHHCHHHHHHHHH
NSLSKRSNGAGLRSGLIAGDRALIDAFLRLRAYASAGMPLPIMEASAALWDDETHVEANR
HHHHCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCHHHHHH
ALYREKIDLAAARLKGMLGFRRPPGGFFLWLDTTPWGETGEQATLRLWRDHGLKVLPGAY
HHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHCCCEEECCCE
MTANPDAGDNPARNRLRLALVHDGPVVARALDRLVAASPH
EECCCCCCCCCCCCCEEEEEEECCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA