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The map label for this gene is capD [H]

Identifier: 83594985

GI number: 83594985

Start: 4196060

End: 4198018

Strand: Reverse

Name: capD [H]

Synonym: Rru_A3656

Alternate gene names: 83594985

Gene position: 4198018-4196060 (Counterclockwise)

Preceding gene: 83594986

Following gene: 83594984

Centisome position: 96.44

GC content: 68.56

Gene sequence:

>1959_bases
ATGCGGTCGCTGTTTACCCCGATGCCGCCCCGGGCGCGCATCGTCTTCGCCCACGACGTGGTGATGGCGGCGCTGTCCTT
CGTGATCTCGCTGTATCTGCGCCTGGGTGACGGCCTGATCCGCTATGTGCCGCTACCCGATCTGGGGCGCGAGGCCGCCC
TGTTCGCCGCTTGCGCGGCGGTCGCCTTTTACACCCAGCGCATGTATGTGGGCATCTGGCGCTATGCCTCGGTCGATGAC
CTGATCGCCCTGTTGCGCGGCGCCACCCTGACCCTGGTGCTGTTCCTGCCCGCCGCCTTTCTCATCACCCGCATGGAATA
CGTGCCCCGGTCGGTGCTGGTCATCAATTGGGTGGTGCTGATGGCCCTGCTGGGCGGCCCGCGCTTTTTGTACCGGCTGT
TCAAGGACCGCAAATTCCAGCTGCGGCGCGCCCGCTCGCGCTCGGCGATCCCGGTCTTGCTGGTCGGGGCCGGTGACGGC
TGCGAGATGTTCTTGCGCTCGGTCGCCCGCGAGACCGACAGCCCCTATCTGCCCGTCGGCATCCTGTCCGAGCAGGAAAC
CCGAGTCGGGCGCAATCTGCGCGGCGTCGAGGTTCTGGGCACCTTGGATCGTCTGGCCGAGATCGTCGTCCGGCTTGAAC
GCTGGGGCGAGCGGCCGCGCAAGCTGATCTTGACCTCCGAGACCCTCGATCCCGCCCAGGTGCGCGGCCTGCTTGACGCC
TGCGACGCCTTGGGGCTGTCGCTGGCCCGGTTGCCCCGGCTGATGGAGCTGCGCGACGGCGGCGCCGATAGCCTGGAGGT
GCGCCCGGTGGCCATCGAGGATCTGCTGGGCCGGCCCCAGGCCGTGCTTGACCGCGCCCCCGTCGTCGCCCTGGTCGGCG
GTCGCCGGGTGCTGGTCACCGGCGCCGGCGGCTCGATCGGCTCGGAGCTGGTGCGCCAGATCGCCGCCCTCGACCCGGCC
CGCCTGATCCTGGCCGACAGCAGCGAATTTGCCCTCTACACCATCGATATGGAGATCAACGAGCGCTATCCGACGCTGTC
GCGCCGGCCGGTGATCGCCGATGTCCGCGATGGCGACCGCGTCGATCGGGTGATGGCCGAGGAAAAGCCCGAGCTGGTGT
TCCACGCCGCCGCCCTCAAGCATGTGCCGATGGTCGAATTCAATCCGCTTGAGGGCCTGCGCACCAATGCCCTGGGCACG
CGCACGGTGGCCGAGGCCTGCCGCCGTCACGGCGTTGGCACCATGGTGCTGATCTCGACCGACAAGGCGGTCAACCCGAC
CAATGTGATGGGCGCCTCCAAGCGCATGGCCGAAAGCTGGTGTCAGGCCCTTGACCTCGCCGAGCGAACGAGCGCGGCGG
CCACCCATTTCGTCACCGTGCGCTTTGGCAATGTGCTGGGATCGACCGGCTCGGTGGTGCCCTTGTTCCAGCGCCAATTG
GCCGCCGGCGGTCCGCTGACCGTGACCCATCCCGAAATCACCCGCTTCTTCATGACCATCCGCGAAGCCGTGGAGCTGGT
CTTGCAGGCCGCCGCCCTGGGGTCGCGCGAGGAAAGCTATCGCGGCTCGCTGTTCGTGCTGGATATGGGCGAGCCGGTGA
AGATCGCCGATCTCGCCCGCCAGATGATCCGTCTGGCTGGCCTCAAGCCCGAGGTCGATGTCCAGATCGCCTATACGGGC
CTGCGTCCGGGCGAGAAGCTGTTCGAAGAGATCTTCCACGGCAAGGAAACCTCGCTGCCCACGCCGACCGACGGCGTGTT
GGTGGCCGCGCCGCGCGTGCCCGATCCGGTGTTCCTGGGCATCCAGTTCGACGCCCTGGCCAGGGCCTGCGCGGCGGGCG
ACGAGGCGCAGGCCCGCGCCGTGATCGCCGCCCTGGTCCCCGAATTCCACAATCCCCCGGCCGAGACCACGCCCCAACCC
TTTGAGGGCGAGTGCGCCCAGGCGCGCGCCGTGCTATAG

Upstream 100 bases:

>100_bases
CGGCCCTGGCCGCGGGCATCGTGGCGATCGCCCTGGTGCTGGGAACCTTCGCCCGCGCCGGACGCGGCCGGACGCAAGCA
AGGGAATACGAGGCGTGAGA

Downstream 100 bases:

>100_bases
CCAGACTCAGCGATCTCCCCGCCGACCAAGCCTTTCCGCCTCCGCCAAGGATCCCGACCGTGCTCCATTCCCTGCCCATC
CGCCGCGCCCTGATCAGCGT

Product: polysaccharide biosynthesis protein CapD

Products: dTDP-4-dehydro-6-deoxy-D-glucose; H2O [C]

Alternate protein names: NA

Number of amino acids: Translated: 652; Mature: 652

Protein sequence:

>652_residues
MRSLFTPMPPRARIVFAHDVVMAALSFVISLYLRLGDGLIRYVPLPDLGREAALFAACAAVAFYTQRMYVGIWRYASVDD
LIALLRGATLTLVLFLPAAFLITRMEYVPRSVLVINWVVLMALLGGPRFLYRLFKDRKFQLRRARSRSAIPVLLVGAGDG
CEMFLRSVARETDSPYLPVGILSEQETRVGRNLRGVEVLGTLDRLAEIVVRLERWGERPRKLILTSETLDPAQVRGLLDA
CDALGLSLARLPRLMELRDGGADSLEVRPVAIEDLLGRPQAVLDRAPVVALVGGRRVLVTGAGGSIGSELVRQIAALDPA
RLILADSSEFALYTIDMEINERYPTLSRRPVIADVRDGDRVDRVMAEEKPELVFHAAALKHVPMVEFNPLEGLRTNALGT
RTVAEACRRHGVGTMVLISTDKAVNPTNVMGASKRMAESWCQALDLAERTSAAATHFVTVRFGNVLGSTGSVVPLFQRQL
AAGGPLTVTHPEITRFFMTIREAVELVLQAAALGSREESYRGSLFVLDMGEPVKIADLARQMIRLAGLKPEVDVQIAYTG
LRPGEKLFEEIFHGKETSLPTPTDGVLVAAPRVPDPVFLGIQFDALARACAAGDEAQARAVIAALVPEFHNPPAETTPQP
FEGECAQARAVL

Sequences:

>Translated_652_residues
MRSLFTPMPPRARIVFAHDVVMAALSFVISLYLRLGDGLIRYVPLPDLGREAALFAACAAVAFYTQRMYVGIWRYASVDD
LIALLRGATLTLVLFLPAAFLITRMEYVPRSVLVINWVVLMALLGGPRFLYRLFKDRKFQLRRARSRSAIPVLLVGAGDG
CEMFLRSVARETDSPYLPVGILSEQETRVGRNLRGVEVLGTLDRLAEIVVRLERWGERPRKLILTSETLDPAQVRGLLDA
CDALGLSLARLPRLMELRDGGADSLEVRPVAIEDLLGRPQAVLDRAPVVALVGGRRVLVTGAGGSIGSELVRQIAALDPA
RLILADSSEFALYTIDMEINERYPTLSRRPVIADVRDGDRVDRVMAEEKPELVFHAAALKHVPMVEFNPLEGLRTNALGT
RTVAEACRRHGVGTMVLISTDKAVNPTNVMGASKRMAESWCQALDLAERTSAAATHFVTVRFGNVLGSTGSVVPLFQRQL
AAGGPLTVTHPEITRFFMTIREAVELVLQAAALGSREESYRGSLFVLDMGEPVKIADLARQMIRLAGLKPEVDVQIAYTG
LRPGEKLFEEIFHGKETSLPTPTDGVLVAAPRVPDPVFLGIQFDALARACAAGDEAQARAVIAALVPEFHNPPAETTPQP
FEGECAQARAVL
>Mature_652_residues
MRSLFTPMPPRARIVFAHDVVMAALSFVISLYLRLGDGLIRYVPLPDLGREAALFAACAAVAFYTQRMYVGIWRYASVDD
LIALLRGATLTLVLFLPAAFLITRMEYVPRSVLVINWVVLMALLGGPRFLYRLFKDRKFQLRRARSRSAIPVLLVGAGDG
CEMFLRSVARETDSPYLPVGILSEQETRVGRNLRGVEVLGTLDRLAEIVVRLERWGERPRKLILTSETLDPAQVRGLLDA
CDALGLSLARLPRLMELRDGGADSLEVRPVAIEDLLGRPQAVLDRAPVVALVGGRRVLVTGAGGSIGSELVRQIAALDPA
RLILADSSEFALYTIDMEINERYPTLSRRPVIADVRDGDRVDRVMAEEKPELVFHAAALKHVPMVEFNPLEGLRTNALGT
RTVAEACRRHGVGTMVLISTDKAVNPTNVMGASKRMAESWCQALDLAERTSAAATHFVTVRFGNVLGSTGSVVPLFQRQL
AAGGPLTVTHPEITRFFMTIREAVELVLQAAALGSREESYRGSLFVLDMGEPVKIADLARQMIRLAGLKPEVDVQIAYTG
LRPGEKLFEEIFHGKETSLPTPTDGVLVAAPRVPDPVFLGIQFDALARACAAGDEAQARAVIAALVPEFHNPPAETTPQP
FEGECAQARAVL

Specific function: Required for the biosynthesis of type 1 capsular polysaccharide [H]

COG id: COG1086

COG function: function code MG; Predicted nucleoside-diphosphate sugar epimerases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polysaccharide synthase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR003869 [H]

Pfam domain/function: PF02719 Polysacc_synt_2 [H]

EC number: 4.2.1.46 [C]

Molecular weight: Translated: 71218; Mature: 71218

Theoretical pI: Translated: 7.81; Mature: 7.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSLFTPMPPRARIVFAHDVVMAALSFVISLYLRLGDGLIRYVPLPDLGREAALFAACAA
CCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHH
VAFYTQRMYVGIWRYASVDDLIALLRGATLTLVLFLPAAFLITRMEYVPRSVLVINWVVL
HHHHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
MALLGGPRFLYRLFKDRKFQLRRARSRSAIPVLLVGAGDGCEMFLRSVARETDSPYLPVG
HHHHCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCCEEE
ILSEQETRVGRNLRGVEVLGTLDRLAEIVVRLERWGERPRKLILTSETLDPAQVRGLLDA
ECCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHH
CDALGLSLARLPRLMELRDGGADSLEVRPVAIEDLLGRPQAVLDRAPVVALVGGRRVLVT
HHHHHHHHHHHHHHHHHCCCCCCCEEECCEEHHHHHCCCHHHHCCCCEEEEECCCEEEEE
GAGGSIGSELVRQIAALDPARLILADSSEFALYTIDMEINERYPTLSRRPVIADVRDGDR
ECCCCHHHHHHHHHHHCCCCEEEEECCCCEEEEEEEEEECCCCCCCCCCCEEEECCCCHH
VDRVMAEEKPELVFHAAALKHVPMVEFNPLEGLRTNALGTRTVAEACRRHGVGTMVLIST
HHHHHHCCCCHHEEEHHHHHCCCCEEECCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEEC
DKAVNPTNVMGASKRMAESWCQALDLAERTSAAATHFVTVRFGNVLGSTGSVVPLFQRQL
CCCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEECHHHCCCCCCHHHHHHHH
AAGGPLTVTHPEITRFFMTIREAVELVLQAAALGSREESYRGSLFVLDMGEPVKIADLAR
CCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCHHHHHHH
QMIRLAGLKPEVDVQIAYTGLRPGEKLFEEIFHGKETSLPTPTDGVLVAAPRVPDPVFLG
HHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCCCEEEE
IQFDALARACAAGDEAQARAVIAALVPEFHNPPAETTPQPFEGECAQARAVL
EEHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHCCCC
>Mature Secondary Structure
MRSLFTPMPPRARIVFAHDVVMAALSFVISLYLRLGDGLIRYVPLPDLGREAALFAACAA
CCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHH
VAFYTQRMYVGIWRYASVDDLIALLRGATLTLVLFLPAAFLITRMEYVPRSVLVINWVVL
HHHHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
MALLGGPRFLYRLFKDRKFQLRRARSRSAIPVLLVGAGDGCEMFLRSVARETDSPYLPVG
HHHHCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCCEEE
ILSEQETRVGRNLRGVEVLGTLDRLAEIVVRLERWGERPRKLILTSETLDPAQVRGLLDA
ECCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHH
CDALGLSLARLPRLMELRDGGADSLEVRPVAIEDLLGRPQAVLDRAPVVALVGGRRVLVT
HHHHHHHHHHHHHHHHHCCCCCCCEEECCEEHHHHHCCCHHHHCCCCEEEEECCCEEEEE
GAGGSIGSELVRQIAALDPARLILADSSEFALYTIDMEINERYPTLSRRPVIADVRDGDR
ECCCCHHHHHHHHHHHCCCCEEEEECCCCEEEEEEEEEECCCCCCCCCCCEEEECCCCHH
VDRVMAEEKPELVFHAAALKHVPMVEFNPLEGLRTNALGTRTVAEACRRHGVGTMVLIST
HHHHHHCCCCHHEEEHHHHHCCCCEEECCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEEC
DKAVNPTNVMGASKRMAESWCQALDLAERTSAAATHFVTVRFGNVLGSTGSVVPLFQRQL
CCCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEECHHHCCCCCCHHHHHHHH
AAGGPLTVTHPEITRFFMTIREAVELVLQAAALGSREESYRGSLFVLDMGEPVKIADLAR
CCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCHHHHHHH
QMIRLAGLKPEVDVQIAYTGLRPGEKLFEEIFHGKETSLPTPTDGVLVAAPRVPDPVFLG
HHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCCCEEEE
IQFDALARACAAGDEAQARAVIAALVPEFHNPPAETTPQPFEGECAQARAVL
EEHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.093 {dTDPglucose}} [C]

Substrates: dTDPglucose [C]

Specific reaction: dTDPglucose --> dTDP-4-dehydro-6-deoxy-D-glucose + H2O [C]

General reaction: Elimination (of H2O C-O bond cleavage [C]

Inhibitor: p-Chloromercuribenzoate; TMP [C]

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7961465 [H]