BacMap

Definition	Rhodospirillum rubrum ATCC 11170 chromosome, complete genome.
Accession	NC_007643
Length	4,352,825

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The map label for this gene is braF [H]

Identifier: 83594839

GI number: 83594839

Start: 4038600

End: 4039424

Strand: Reverse

Name: braF [H]

Synonym: Rru_A3510

Alternate gene names: 83594839

Gene position: 4039424-4038600 (Counterclockwise)

Preceding gene: 83594842

Following gene: 83594838

Centisome position: 92.8

GC content: 65.94

Gene sequence:

>825_bases
ATGTCCAAGGATTCGCCGATCCTGGAAGTGCGCGACGTGTCCCTTCATTTCGGGGGGGTACGGGCCTTGACCATGGTCTC
GACCACGGTCGCCGCCGGCGCCTTGTTCGCCATCATCGGCCCCAATGGGGCGGGCAAGACCTCGATGATGAACTGCATCT
CGGGGCGCTACCGGCCGACCCAGGGCAAGGTGCTGTTCAAGGGGCGCGACGTCACCGCCCTGCCGCCCGATCGCCGCACC
GGGCTTGGCCTGGGCCGGACCTTCCAGAACCTCGCGCTGTTTGGCCATATGAGCGTGCTCGACAACATCATGGTCGGGCG
CCATCACCTGCTGCGCAACGGCTTCGTCGGCGGGGCGCTCTATTGGCTGGGCGGGACCCAGGCCGAGGAGCTGGAGCACC
GCCGCGCCGTCGAGGACATCATCGATTTCCTGGAGATCGGCGCCATCCGCAAGGCCACCGCCGGCACGCTGTCCTATGGG
TTGCGCAAGCGGGTCGAACTGGCCCGGGCGATCGCGCTTAAACCCGACCTGATCTTGCTCGACGAGCCGATGGCCGGCAT
GAACCAGGAGGAAAAGGAGGACATGGCCCGCTTCATCGTCGATCTCAACGAGGAATGGGGGATGACGGTGATCATGATCG
AGCACGACATGGGCGTGGTCATGGATATCAGCCATACGGTGATGGTGCTCGATTTCGGCCAGAAGATCGCCGAGGGGCCG
CCAGCGGCGATCATGGCCCATCCCCAGGTGCGCCGGGCCTATCTCGGCGAGAACGACGACGACGCGGCGGTGCCACCCGC
CAAGACCGCCGGGGAGACCGCCTGA

Upstream 100 bases:

>100_bases
TTGCGTTAAGTCGGTAGTCGAGAAAGCCGCTCAACCACCAAAACCGGTCCGGTCTAACGAGTCCGGGGGGAAGAGCGGCC
GACAAGGGAGGCCGCTGTTG

Downstream 100 bases:

>100_bases
TGGCATCCAGACGTTCCCGCCGCGCCCCCGAGTATGCCGATGTCCACCGGCTCGACACCTTTCCCAAGGTTCTGGCCGAC
AACGCCAAACGCTTCCCCCA

Product: ABC transporter protein

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 274; Mature: 273

Protein sequence:

>274_residues
MSKDSPILEVRDVSLHFGGVRALTMVSTTVAAGALFAIIGPNGAGKTSMMNCISGRYRPTQGKVLFKGRDVTALPPDRRT
GLGLGRTFQNLALFGHMSVLDNIMVGRHHLLRNGFVGGALYWLGGTQAEELEHRRAVEDIIDFLEIGAIRKATAGTLSYG
LRKRVELARAIALKPDLILLDEPMAGMNQEEKEDMARFIVDLNEEWGMTVIMIEHDMGVVMDISHTVMVLDFGQKIAEGP
PAAIMAHPQVRRAYLGENDDDAAVPPAKTAGETA

Sequences:

>Translated_274_residues
MSKDSPILEVRDVSLHFGGVRALTMVSTTVAAGALFAIIGPNGAGKTSMMNCISGRYRPTQGKVLFKGRDVTALPPDRRT
GLGLGRTFQNLALFGHMSVLDNIMVGRHHLLRNGFVGGALYWLGGTQAEELEHRRAVEDIIDFLEIGAIRKATAGTLSYG
LRKRVELARAIALKPDLILLDEPMAGMNQEEKEDMARFIVDLNEEWGMTVIMIEHDMGVVMDISHTVMVLDFGQKIAEGP
PAAIMAHPQVRRAYLGENDDDAAVPPAKTAGETA
>Mature_273_residues
SKDSPILEVRDVSLHFGGVRALTMVSTTVAAGALFAIIGPNGAGKTSMMNCISGRYRPTQGKVLFKGRDVTALPPDRRTG
LGLGRTFQNLALFGHMSVLDNIMVGRHHLLRNGFVGGALYWLGGTQAEELEHRRAVEDIIDFLEIGAIRKATAGTLSYGL
RKRVELARAIALKPDLILLDEPMAGMNQEEKEDMARFIVDLNEEWGMTVIMIEHDMGVVMDISHTVMVLDFGQKIAEGPP
AAIMAHPQVRRAYLGENDDDAAVPPAKTAGETA

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0411

COG function: function code E; ABC-type branched-chain amino acid transport systems, ATPase component

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transporter domain [H]

Homologues:

Organism=Homo sapiens, GI27881501, Length=199, Percent_Identity=25.6281407035176, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI30795238, Length=199, Percent_Identity=25.6281407035176, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI105990541, Length=214, Percent_Identity=27.1028037383178, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI156105685, Length=262, Percent_Identity=27.0992366412214, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI46592956, Length=225, Percent_Identity=25.7777777777778, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI46592971, Length=225, Percent_Identity=25.7777777777778, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI46592898, Length=225, Percent_Identity=25.7777777777778, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI46592978, Length=225, Percent_Identity=25.7777777777778, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI46592964, Length=225, Percent_Identity=25.7777777777778, Blast_Score=69, Evalue=6e-12,
Organism=Homo sapiens, GI46592915, Length=225, Percent_Identity=25.7777777777778, Blast_Score=69, Evalue=6e-12,
Organism=Homo sapiens, GI45446740, Length=220, Percent_Identity=25, Blast_Score=68, Evalue=8e-12,
Organism=Homo sapiens, GI47078218, Length=220, Percent_Identity=25, Blast_Score=68, Evalue=8e-12,
Organism=Homo sapiens, GI31657092, Length=242, Percent_Identity=23.5537190082645, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1789864, Length=261, Percent_Identity=36.3984674329502, Blast_Score=180, Evalue=8e-47,
Organism=Escherichia coli, GI87081791, Length=247, Percent_Identity=29.9595141700405, Blast_Score=134, Evalue=9e-33,
Organism=Escherichia coli, GI1788761, Length=245, Percent_Identity=28.5714285714286, Blast_Score=119, Evalue=1e-28,
Organism=Escherichia coli, GI87081709, Length=261, Percent_Identity=28.3524904214559, Blast_Score=116, Evalue=1e-27,
Organism=Escherichia coli, GI1787029, Length=247, Percent_Identity=28.7449392712551, Blast_Score=115, Evalue=4e-27,
Organism=Escherichia coli, GI1787712, Length=227, Percent_Identity=29.9559471365639, Blast_Score=113, Evalue=1e-26,
Organism=Escherichia coli, GI1789593, Length=251, Percent_Identity=30.6772908366534, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI1786398, Length=232, Percent_Identity=28.0172413793103, Blast_Score=111, Evalue=6e-26,
Organism=Escherichia coli, GI1788472, Length=238, Percent_Identity=28.5714285714286, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1787370, Length=245, Percent_Identity=26.1224489795918, Blast_Score=103, Evalue=9e-24,
Organism=Escherichia coli, GI1790190, Length=238, Percent_Identity=28.5714285714286, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1789032, Length=227, Percent_Identity=29.5154185022026, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1789672, Length=258, Percent_Identity=25.1937984496124, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI1788210, Length=275, Percent_Identity=26.5454545454545, Blast_Score=98, Evalue=4e-22,
Organism=Escherichia coli, GI1790467, Length=244, Percent_Identity=25.8196721311475, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1789586, Length=269, Percent_Identity=30.1115241635688, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1786872, Length=252, Percent_Identity=25.3968253968254, Blast_Score=95, Evalue=4e-21,
Organism=Escherichia coli, GI1789991, Length=232, Percent_Identity=27.5862068965517, Blast_Score=95, Evalue=4e-21,
Organism=Escherichia coli, GI87082267, Length=231, Percent_Identity=26.8398268398268, Blast_Score=94, Evalue=8e-21,
Organism=Escherichia coli, GI87082268, Length=253, Percent_Identity=27.2727272727273, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1788225, Length=254, Percent_Identity=28.3464566929134, Blast_Score=91, Evalue=8e-20,
Organism=Escherichia coli, GI1790525, Length=248, Percent_Identity=25.8064516129032, Blast_Score=91, Evalue=8e-20,
Organism=Escherichia coli, GI1786253, Length=192, Percent_Identity=29.6875, Blast_Score=90, Evalue=1e-19,
Organism=Escherichia coli, GI48995001, Length=227, Percent_Identity=27.3127753303965, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1786563, Length=227, Percent_Identity=26.431718061674, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1788644, Length=261, Percent_Identity=29.1187739463602, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1786319, Length=241, Percent_Identity=28.2157676348548, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI48994997, Length=248, Percent_Identity=25.4032258064516, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1788450, Length=250, Percent_Identity=24, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1789873, Length=226, Percent_Identity=29.2035398230088, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1786698, Length=220, Percent_Identity=26.3636363636364, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1786345, Length=240, Percent_Identity=25.8333333333333, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1790544, Length=246, Percent_Identity=26.0162601626016, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1787105, Length=255, Percent_Identity=27.0588235294118, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI145693107, Length=261, Percent_Identity=26.4367816091954, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI48994943, Length=269, Percent_Identity=23.4200743494424, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1788506, Length=238, Percent_Identity=27.3109243697479, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI87081782, Length=241, Percent_Identity=25.3112033195021, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI1790739, Length=272, Percent_Identity=25.3676470588235, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1789962, Length=259, Percent_Identity=22.7799227799228, Blast_Score=78, Evalue=5e-16,
Organism=Escherichia coli, GI1786803, Length=254, Percent_Identity=24.0157480314961, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI1786981, Length=220, Percent_Identity=29.5454545454545, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1787548, Length=262, Percent_Identity=25.1908396946565, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1787089, Length=247, Percent_Identity=23.4817813765182, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1786703, Length=198, Percent_Identity=27.7777777777778, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1788053, Length=232, Percent_Identity=26.7241379310345, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI1787759, Length=284, Percent_Identity=26.056338028169, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1789963, Length=258, Percent_Identity=24.8062015503876, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1787758, Length=263, Percent_Identity=23.574144486692, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1788897, Length=234, Percent_Identity=25.2136752136752, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI87081834, Length=218, Percent_Identity=26.605504587156, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1787041, Length=266, Percent_Identity=25.9398496240602, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1787792, Length=230, Percent_Identity=24.7826086956522, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1787972, Length=186, Percent_Identity=25.8064516129032, Blast_Score=65, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI71983516, Length=224, Percent_Identity=25.8928571428571, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI71983510, Length=224, Percent_Identity=25.8928571428571, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI71983523, Length=224, Percent_Identity=25.8928571428571, Blast_Score=72, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17510237, Length=227, Percent_Identity=23.7885462555066, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI115533608, Length=201, Percent_Identity=27.8606965174129, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24650855, Length=229, Percent_Identity=25.3275109170306, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24650853, Length=229, Percent_Identity=25.3275109170306, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI281362751, Length=229, Percent_Identity=25.3275109170306, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24666092, Length=228, Percent_Identity=23.6842105263158, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI221512771, Length=234, Percent_Identity=25.6410256410256, Blast_Score=72, Evalue=6e-13,
Organism=Drosophila melanogaster, GI24582014, Length=245, Percent_Identity=24.4897959183673, Blast_Score=65, Evalue=5e-11,
Organism=Drosophila melanogaster, GI24582012, Length=245, Percent_Identity=24.4897959183673, Blast_Score=65, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003439
- InterPro:   IPR017871
- InterPro:   IPR003593 [H]

Pfam domain/function: PF00005 ABC_tran [H]

EC number: NA

Molecular weight: Translated: 29762; Mature: 29631

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: PS50893 ABC_TRANSPORTER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
5.5 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKDSPILEVRDVSLHFGGVRALTMVSTTVAAGALFAIIGPNGAGKTSMMNCISGRYRPT
CCCCCCEEEEEEEEEECCCHHHHHHHHHHHHHCEEEEEECCCCCCHHHHHHHHCCCCCCC
QGKVLFKGRDVTALPPDRRTGLGLGRTFQNLALFGHMSVLDNIMVGRHHLLRNGFVGGAL
CCEEEEECCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEE
YWLGGTQAEELEHRRAVEDIIDFLEIGAIRKATAGTLSYGLRKRVELARAIALKPDLILL
EEECCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
DEPMAGMNQEEKEDMARFIVDLNEEWGMTVIMIEHDMGVVMDISHTVMVLDFGQKIAEGP
CCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCEEEECCCEEEEEECCHHHHCCC
PAAIMAHPQVRRAYLGENDDDAAVPPAKTAGETA
CCEEEECHHHHHHHCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SKDSPILEVRDVSLHFGGVRALTMVSTTVAAGALFAIIGPNGAGKTSMMNCISGRYRPT
CCCCCEEEEEEEEEECCCHHHHHHHHHHHHHCEEEEEECCCCCCHHHHHHHHCCCCCCC
QGKVLFKGRDVTALPPDRRTGLGLGRTFQNLALFGHMSVLDNIMVGRHHLLRNGFVGGAL
CCEEEEECCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEE
YWLGGTQAEELEHRRAVEDIIDFLEIGAIRKATAGTLSYGLRKRVELARAIALKPDLILL
EEECCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
DEPMAGMNQEEKEDMARFIVDLNEEWGMTVIMIEHDMGVVMDISHTVMVLDFGQKIAEGP
CCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCEEEECCCEEEEEECCHHHHCCC
PAAIMAHPQVRRAYLGENDDDAAVPPAKTAGETA
CCEEEECHHHHHHHCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]