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The map label for this gene is dmlR [H]

Identifier: 83594805

GI number: 83594805

Start: 3999922

End: 4000803

Strand: Reverse

Name: dmlR [H]

Synonym: Rru_A3476

Alternate gene names: 83594805

Gene position: 4000803-3999922 (Counterclockwise)

Preceding gene: 83594807

Following gene: 83594803

Centisome position: 91.91

GC content: 69.95

Gene sequence:

>882_bases
ATGGATACGGAGGCCGTCACCGTTTTCGTCACCGCCGCCGCGGCCGGCAGCCTGTCCGCCGCCGCCCGCCGGCTGGCGAT
CACACCGATGACGGCGACGCGCCGTCTTGCCGGGTTGGAGCGGGTGCTTGGCCTGCGGCTGATGCAGCGCACCACGCGCG
CGGTGTCGCTTACCCCCGAGGGTGAGGCTTTCCTGCCCTATGCCCAGGCCATGGTGGAAAGCGAAGCCGCCGGGCGGGCG
ATGCTGCGTTCGGCGGCCATCGGTGTTTCGGGACTGCTGCGCGTCACCACCCCGGCGGCCTTTGGCCGCAAGATCATCGC
CCCGCTGATTCCCGGCTTGCTGCGCGCCCATCCCGAGCTGCGCATCGATCTTGATCTCAATGACGCGGTTGTCGATATCG
TCGCCACCGGCGCCGATCTGGCGATCCGCATCGCCGTTTTGCGCGATAGCGCCCTGATCGCCCACAAGGTGGCGGCCAGC
CCCCGGGTGTTATGCGCCGCCCCCGCCTACCTCGCCGAACGCGGCACCCCGCGCCTGCTCGCCGATCTGGCCGGCCACGA
CTGCCTGACCCAAACCGGCACCACCCATTGGCCCTTCCAGGCCGACCGCCGCGATCTCAAGGCGCGGGTCAACGGACGGT
TTTCGGCGAACGGCATCGAAGCCCTGCACGGCGCCTGCCTGGGTGGCGCCGGCATCGCCCTGCTTGCTTTGTGGAACGTC
GGCGACGATCTGGCGGCGGGTCGGCTGGTGCGGATCACCCTGGAGGACGCCGCCGCCCTTGATCTGTCGATCTGGGCCGT
CTATCCCAGCGCCCGGTTCGTCCTGCCCAAGCTGCGGGTTTTCATCCAGGCCCTGGAAGGGGCCTTGCGCCCGGCGGCTT
AG

Upstream 100 bases:

>100_bases
GACCTTTTTGGCGGGATCTGGGATGCCGAAGTGATAGCCCCCTCAACGGTTTTCGTGTAGGTGGTGGAAGTGAGCAGCCT
GTATATCGGAAAGTGATAGA

Downstream 100 bases:

>100_bases
GCCAATCCGCTTTATGCGGCGCGGGCCATCGCCTGGATGGTGGCGATCATGCTGGCCACGCCGTTGCGGCGGTTGGGGCT
GATGTGGCTGTCCAGGCCAA

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: D-malate degradation protein R [H]

Number of amino acids: Translated: 293; Mature: 293

Protein sequence:

>293_residues
MDTEAVTVFVTAAAAGSLSAAARRLAITPMTATRRLAGLERVLGLRLMQRTTRAVSLTPEGEAFLPYAQAMVESEAAGRA
MLRSAAIGVSGLLRVTTPAAFGRKIIAPLIPGLLRAHPELRIDLDLNDAVVDIVATGADLAIRIAVLRDSALIAHKVAAS
PRVLCAAPAYLAERGTPRLLADLAGHDCLTQTGTTHWPFQADRRDLKARVNGRFSANGIEALHGACLGGAGIALLALWNV
GDDLAAGRLVRITLEDAAALDLSIWAVYPSARFVLPKLRVFIQALEGALRPAA

Sequences:

>Translated_293_residues
MDTEAVTVFVTAAAAGSLSAAARRLAITPMTATRRLAGLERVLGLRLMQRTTRAVSLTPEGEAFLPYAQAMVESEAAGRA
MLRSAAIGVSGLLRVTTPAAFGRKIIAPLIPGLLRAHPELRIDLDLNDAVVDIVATGADLAIRIAVLRDSALIAHKVAAS
PRVLCAAPAYLAERGTPRLLADLAGHDCLTQTGTTHWPFQADRRDLKARVNGRFSANGIEALHGACLGGAGIALLALWNV
GDDLAAGRLVRITLEDAAALDLSIWAVYPSARFVLPKLRVFIQALEGALRPAA
>Mature_293_residues
MDTEAVTVFVTAAAAGSLSAAARRLAITPMTATRRLAGLERVLGLRLMQRTTRAVSLTPEGEAFLPYAQAMVESEAAGRA
MLRSAAIGVSGLLRVTTPAAFGRKIIAPLIPGLLRAHPELRIDLDLNDAVVDIVATGADLAIRIAVLRDSALIAHKVAAS
PRVLCAAPAYLAERGTPRLLADLAGHDCLTQTGTTHWPFQADRRDLKARVNGRFSANGIEALHGACLGGAGIALLALWNV
GDDLAAGRLVRITLEDAAALDLSIWAVYPSARFVLPKLRVFIQALEGALRPAA

Specific function: Transcriptional regulator required for the aerobic growth on D-malate as the sole carbon source. Induces the expression of dmlA in response to D-malate or L- or meso-tartrate. Negatively regulates its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081978, Length=282, Percent_Identity=34.0425531914894, Blast_Score=159, Evalue=2e-40,
Organism=Escherichia coli, GI1789440, Length=284, Percent_Identity=33.8028169014084, Blast_Score=149, Evalue=3e-37,
Organism=Escherichia coli, GI1786401, Length=293, Percent_Identity=30.7167235494881, Blast_Score=140, Evalue=8e-35,
Organism=Escherichia coli, GI145693193, Length=302, Percent_Identity=30.794701986755, Blast_Score=124, Evalue=8e-30,
Organism=Escherichia coli, GI1787128, Length=293, Percent_Identity=27.6450511945392, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1789639, Length=283, Percent_Identity=27.9151943462898, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI1787589, Length=285, Percent_Identity=27.719298245614, Blast_Score=101, Evalue=7e-23,
Organism=Escherichia coli, GI1786448, Length=275, Percent_Identity=24.3636363636364, Blast_Score=61, Evalue=1e-10,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 30825; Mature: 30825

Theoretical pI: Translated: 10.05; Mature: 10.05

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTEAVTVFVTAAAAGSLSAAARRLAITPMTATRRLAGLERVLGLRLMQRTTRAVSLTPE
CCCCEEEEEEEECCCCCHHHHHHHEEECCCHHHHHHHHHHHHHHHHHHHHHHHEEEECCC
GEAFLPYAQAMVESEAAGRAMLRSAAIGVSGLLRVTTPAAFGRKIIAPLIPGLLRAHPEL
CCEEHHHHHHHHHHHHHHHHHHHHHHCCHHHHEEECCCHHHHHHHHHHHHHHHHHCCCCE
RIDLDLNDAVVDIVATGADLAIRIAVLRDSALIAHKVAASPRVLCAAPAYLAERGTPRLL
EEEECCCHHEEEEEECCCCCEEEEEEEECCHHHHHHHCCCCCEEEECCHHHHHCCCHHHH
ADLAGHDCLTQTGTTHWPFQADRRDLKARVNGRFSANGIEALHGACLGGAGIALLALWNV
HHHCCCCHHHCCCCCCCCCCCCHHHHHHHHCCEECCCHHHHHHHHHHCCCCEEEHEEECC
GDDLAAGRLVRITLEDAAALDLSIWAVYPSARFVLPKLRVFIQALEGALRPAA
CCCCCCCEEEEEEECCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MDTEAVTVFVTAAAAGSLSAAARRLAITPMTATRRLAGLERVLGLRLMQRTTRAVSLTPE
CCCCEEEEEEEECCCCCHHHHHHHEEECCCHHHHHHHHHHHHHHHHHHHHHHHEEEECCC
GEAFLPYAQAMVESEAAGRAMLRSAAIGVSGLLRVTTPAAFGRKIIAPLIPGLLRAHPEL
CCEEHHHHHHHHHHHHHHHHHHHHHHCCHHHHEEECCCHHHHHHHHHHHHHHHHHCCCCE
RIDLDLNDAVVDIVATGADLAIRIAVLRDSALIAHKVAASPRVLCAAPAYLAERGTPRLL
EEEECCCHHEEEEEECCCCCEEEEEEEECCHHHHHHHCCCCCEEEECCHHHHHCCCHHHH
ADLAGHDCLTQTGTTHWPFQADRRDLKARVNGRFSANGIEALHGACLGGAGIALLALWNV
HHHCCCCHHHCCCCCCCCCCCCHHHHHHHHCCEECCCHHHHHHHHHHCCCCEEEHEEECC
GDDLAAGRLVRITLEDAAALDLSIWAVYPSARFVLPKLRVFIQALEGALRPAA
CCCCCCCEEEEEEECCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503 [H]