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The map label for this gene is ptsI [H]

Identifier: 83594774

GI number: 83594774

Start: 3969015

End: 3970838

Strand: Reverse

Name: ptsI [H]

Synonym: Rru_A3445

Alternate gene names: 83594774

Gene position: 3970838-3969015 (Counterclockwise)

Preceding gene: 83594775

Following gene: 83594773

Centisome position: 91.22

GC content: 68.04

Gene sequence:

>1824_bases
ATGAAGAGTGACGCGCCAAGCGACCGTCCGTCCGTCGATCGCCCGCCGACCCTGCCGCCGCGGGCCTTGCGGCTGAGCGG
TTTGGGCGTCGGGCCGGGGATCTGCGTCGGCACCGTCCATCTCCATGAAATCGGCTCGGTTCCCGTTCCCGAATACAAAA
TCCCCGCCGCCCGCGTCGAAGACGAATGCGCCCGGCTGGCCGAAGCGGCCGAACAGGGCAGCCGTCAGCTTGGCATGCTG
CGCGGCCGGGCCCGCGAGTTGCCGGGCGGCGCCGGCGAGGAACTGGGCTATCTGCTTGACGCCTATCAGAACATGCTCAA
GGGCTCGCGGCTGCTGCGCGGCGTCGAGTCCCGCATCATCAACGAAAAGATCAATGCCGAAGCGGCCATCCGCAACGAGA
TGGACGCCATGGCCGACGTCTTCTCGGCCATGCAAGACAGCTATCTGGCCTCGCGCATCGAGGATGTGAAGGATGTCGGC
TGGCGGCTGATCCGGGTGCTGACCAAGGCCGCCTATCGCCCCTTCACCTTGCTGCCGCGCAACGCCGTGATCGTCGCGGG
CGAACTCAGCCCGGCCGACACCGCCCTGCTTGATCCCAAGCAGGTGGCCGGCATGGCGCTGTCAAGCGGCGGCGCCGATT
CCCACACCGCGATCATGGCCCGTTCCCTGGGCCTGCCCGCCGTGCTCGCCATCGCCGATCTGATGCGCCATGTGCGCTCG
GGCGATATCATCTGCCTGGACGGTCGCACCGGCGAGGTGGTGATCGATCCCGACCGCGAGACCCTGGCCGATTTTCGCAA
GCGCCGCGCCGATTTCCTGCGCGGCCGGCGCAATCTGGGGCGGCTGCGCGATTTGCCGGCCGAAACCCGCGATGGCGCCC
GGGTGCGGCTGATGGCCAATGTCGAGCTTCCCGCCGAGGTTGAAAGCGCCCGCCAGTCGGGGGCCGAGGGCATCGGCCTG
CTGCGCAGCGAATTCATGTTCATGAACCGCACCGTCCTGCCCGACGAGGAGGAGCAGTATCATTTCCTGCGGGGCATGGT
CGCGGGCATGGCCGGTCGGCCGGTCACCGTGCGCACGCTCGATGTCGGCGGCGACAAGTTGAGCGATGCCCTGGGCCTGC
GCGACTGCGCCAATCCGGCGCTTGGCCTGCGCGCCATCCGCCTGTCGCTCAGCCGCCCCGAATTGCTCGAGATCCAGCTC
GCCGCCATCTTGCGGGCCGGCGTCCACGGGCCGGTGCGTATTTTGCTGCCGATGATCAGCACGGTCGAGGAGCTTGACGC
GGTGCGGGAGATCCACGCCAAGGTGGTGGCGCGGCTGCGCCAGCGCGGCGTTCCCTTGCCCGAGCCCCTGCCGCCGCTTG
GCGTGATGATCGAGGTTCCGGGCGCGGCGCTGTCGGCCGACGCCCTGGCGTGGCGCTGCGATTTCTTCGCCATCGGCACC
AACGACCTGACCCAATACACCCTGGCCATCGACCGCTCCGACGAATCGGTCGCCCATCTTTACAACCCCCTGCATCCCGC
CGTGCTGCGGCTGATCCAATTCACCGTGGAAGCCGCCGAGCGCGCCGGCATTCCGGTCTCGGTCTGTGGCGAGATGGCCG
GCGATCCGCGGCTGGCGGCGGTTCTGGTCGGCCTGGGCGTGACCGAGTTGTCGATGTCGGCGGCCAATATCCCGCGGGTC
AAACAAAACATCCGCCGGCTGTCGATGATCGATGTCGCCCGCCAGATCCAGGCGATCATGGCGATCAACGACGCGCCGCG
AATCGCCGCCCTGGCCGATGCCCTGCTGATCGAAAGCGACCGCGTCAGCGAAAAATTGGCCTGA

Upstream 100 bases:

>100_bases
TTGGCCGCCGGACCGGGGACCACCATCTCGCTCTCCGCGATTGGCGAGGACGCCGATACCGCGCTGGAGGCGCTGTGCGC
CCTGATCGCGGACGGGTTCC

Downstream 100 bases:

>100_bases
GGGAGACTTTCGCCCTCGGCTAAAATCTACTTGATAAGGATATAAAGATATCTTTATATTCCCGCCGCCGCCCTCGCATG
CGGCCCCTTCCCTATTGCGG

Product: phosphoenolpyruvate--protein phosphotransferase

Products: NA

Alternate protein names: Phosphotransferase system, enzyme I [H]

Number of amino acids: Translated: 607; Mature: 607

Protein sequence:

>607_residues
MKSDAPSDRPSVDRPPTLPPRALRLSGLGVGPGICVGTVHLHEIGSVPVPEYKIPAARVEDECARLAEAAEQGSRQLGML
RGRARELPGGAGEELGYLLDAYQNMLKGSRLLRGVESRIINEKINAEAAIRNEMDAMADVFSAMQDSYLASRIEDVKDVG
WRLIRVLTKAAYRPFTLLPRNAVIVAGELSPADTALLDPKQVAGMALSSGGADSHTAIMARSLGLPAVLAIADLMRHVRS
GDIICLDGRTGEVVIDPDRETLADFRKRRADFLRGRRNLGRLRDLPAETRDGARVRLMANVELPAEVESARQSGAEGIGL
LRSEFMFMNRTVLPDEEEQYHFLRGMVAGMAGRPVTVRTLDVGGDKLSDALGLRDCANPALGLRAIRLSLSRPELLEIQL
AAILRAGVHGPVRILLPMISTVEELDAVREIHAKVVARLRQRGVPLPEPLPPLGVMIEVPGAALSADALAWRCDFFAIGT
NDLTQYTLAIDRSDESVAHLYNPLHPAVLRLIQFTVEAAERAGIPVSVCGEMAGDPRLAAVLVGLGVTELSMSAANIPRV
KQNIRRLSMIDVARQIQAIMAINDAPRIAALADALLIESDRVSEKLA

Sequences:

>Translated_607_residues
MKSDAPSDRPSVDRPPTLPPRALRLSGLGVGPGICVGTVHLHEIGSVPVPEYKIPAARVEDECARLAEAAEQGSRQLGML
RGRARELPGGAGEELGYLLDAYQNMLKGSRLLRGVESRIINEKINAEAAIRNEMDAMADVFSAMQDSYLASRIEDVKDVG
WRLIRVLTKAAYRPFTLLPRNAVIVAGELSPADTALLDPKQVAGMALSSGGADSHTAIMARSLGLPAVLAIADLMRHVRS
GDIICLDGRTGEVVIDPDRETLADFRKRRADFLRGRRNLGRLRDLPAETRDGARVRLMANVELPAEVESARQSGAEGIGL
LRSEFMFMNRTVLPDEEEQYHFLRGMVAGMAGRPVTVRTLDVGGDKLSDALGLRDCANPALGLRAIRLSLSRPELLEIQL
AAILRAGVHGPVRILLPMISTVEELDAVREIHAKVVARLRQRGVPLPEPLPPLGVMIEVPGAALSADALAWRCDFFAIGT
NDLTQYTLAIDRSDESVAHLYNPLHPAVLRLIQFTVEAAERAGIPVSVCGEMAGDPRLAAVLVGLGVTELSMSAANIPRV
KQNIRRLSMIDVARQIQAIMAINDAPRIAALADALLIESDRVSEKLA
>Mature_607_residues
MKSDAPSDRPSVDRPPTLPPRALRLSGLGVGPGICVGTVHLHEIGSVPVPEYKIPAARVEDECARLAEAAEQGSRQLGML
RGRARELPGGAGEELGYLLDAYQNMLKGSRLLRGVESRIINEKINAEAAIRNEMDAMADVFSAMQDSYLASRIEDVKDVG
WRLIRVLTKAAYRPFTLLPRNAVIVAGELSPADTALLDPKQVAGMALSSGGADSHTAIMARSLGLPAVLAIADLMRHVRS
GDIICLDGRTGEVVIDPDRETLADFRKRRADFLRGRRNLGRLRDLPAETRDGARVRLMANVELPAEVESARQSGAEGIGL
LRSEFMFMNRTVLPDEEEQYHFLRGMVAGMAGRPVTVRTLDVGGDKLSDALGLRDCANPALGLRAIRLSLSRPELLEIQL
AAILRAGVHGPVRILLPMISTVEELDAVREIHAKVVARLRQRGVPLPEPLPPLGVMIEVPGAALSADALAWRCDFFAIGT
NDLTQYTLAIDRSDESVAHLYNPLHPAVLRLIQFTVEAAERAGIPVSVCGEMAGDPRLAAVLVGLGVTELSMSAANIPRV
KQNIRRLSMIDVARQIQAIMAINDAPRIAALADALLIESDRVSEKLA

Specific function: General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their tr

COG id: COG1080

COG function: function code G; Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=547, Percent_Identity=37.6599634369287, Blast_Score=366, Evalue=1e-102,
Organism=Escherichia coli, GI1789193, Length=580, Percent_Identity=33.9655172413793, Blast_Score=306, Evalue=3e-84,
Organism=Escherichia coli, GI48994992, Length=498, Percent_Identity=35.3413654618474, Blast_Score=276, Evalue=3e-75,
Organism=Escherichia coli, GI1788726, Length=623, Percent_Identity=31.3001605136437, Blast_Score=253, Evalue=3e-68,
Organism=Escherichia coli, GI1787994, Length=420, Percent_Identity=26.4285714285714, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI226510935, Length=195, Percent_Identity=24.6153846153846, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 65586; Mature: 65586

Theoretical pI: Translated: 6.62; Mature: 6.62

Prosite motif: PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSDAPSDRPSVDRPPTLPPRALRLSGLGVGPGICVGTVHLHEIGSVPVPEYKIPAARVE
CCCCCCCCCCCCCCCCCCCCHHEEECCCCCCCCCEEEEEEHHHCCCCCCCCCCCCHHHHH
DECARLAEAAEQGSRQLGMLRGRARELPGGAGEELGYLLDAYQNMLKGSRLLRGVESRII
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NEKINAEAAIRNEMDAMADVFSAMQDSYLASRIEDVKDVGWRLIRVLTKAAYRPFTLLPR
HHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCC
NAVIVAGELSPADTALLDPKQVAGMALSSGGADSHTAIMARSLGLPAVLAIADLMRHVRS
CEEEEEECCCCCCHHHCCHHHHHHHHHCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHCC
GDIICLDGRTGEVVIDPDRETLADFRKRRADFLRGRRNLGRLRDLPAETRDGARVRLMAN
CCEEEEECCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEE
VELPAEVESARQSGAEGIGLLRSEFMFMNRTVLPDEEEQYHFLRGMVAGMAGRPVTVRTL
CCCCHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEE
DVGGDKLSDALGLRDCANPALGLRAIRLSLSRPELLEIQLAAILRAGVHGPVRILLPMIS
ECCCHHHHHHHCHHHHCCHHHHHHHHHEECCCCHHHHHHHHHHHHHCCCCHHHHHHHHHH
TVEELDAVREIHAKVVARLRQRGVPLPEPLPPLGVMIEVPGAALSADALAWRCDFFAIGT
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCHHCHHHHEEEEEEEEECC
NDLTQYTLAIDRSDESVAHLYNPLHPAVLRLIQFTVEAAERAGIPVSVCGEMAGDPRLAA
CCCEEEEEEEECCCHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCHHHH
VLVGLGVTELSMSAANIPRVKQNIRRLSMIDVARQIQAIMAINDAPRIAALADALLIESD
HHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCH
RVSEKLA
HHHHHCC
>Mature Secondary Structure
MKSDAPSDRPSVDRPPTLPPRALRLSGLGVGPGICVGTVHLHEIGSVPVPEYKIPAARVE
CCCCCCCCCCCCCCCCCCCCHHEEECCCCCCCCCEEEEEEHHHCCCCCCCCCCCCHHHHH
DECARLAEAAEQGSRQLGMLRGRARELPGGAGEELGYLLDAYQNMLKGSRLLRGVESRII
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NEKINAEAAIRNEMDAMADVFSAMQDSYLASRIEDVKDVGWRLIRVLTKAAYRPFTLLPR
HHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCC
NAVIVAGELSPADTALLDPKQVAGMALSSGGADSHTAIMARSLGLPAVLAIADLMRHVRS
CEEEEEECCCCCCHHHCCHHHHHHHHHCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHCC
GDIICLDGRTGEVVIDPDRETLADFRKRRADFLRGRRNLGRLRDLPAETRDGARVRLMAN
CCEEEEECCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEE
VELPAEVESARQSGAEGIGLLRSEFMFMNRTVLPDEEEQYHFLRGMVAGMAGRPVTVRTL
CCCCHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEE
DVGGDKLSDALGLRDCANPALGLRAIRLSLSRPELLEIQLAAILRAGVHGPVRILLPMIS
ECCCHHHHHHHCHHHHCCHHHHHHHHHEECCCCHHHHHHHHHHHHHCCCCHHHHHHHHHH
TVEELDAVREIHAKVVARLRQRGVPLPEPLPPLGVMIEVPGAALSADALAWRCDFFAIGT
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCHHCHHHHEEEEEEEEECC
NDLTQYTLAIDRSDESVAHLYNPLHPAVLRLIQFTVEAAERAGIPVSVCGEMAGDPRLAA
CCCEEEEEEEECCCHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCHHHH
VLVGLGVTELSMSAANIPRVKQNIRRLSMIDVARQIQAIMAINDAPRIAALADALLIESD
HHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCH
RVSEKLA
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA