Click here to switch to the map view.

The map label for this gene is cysU [H]

Identifier: 83594730

GI number: 83594730

Start: 3917059

End: 3917910

Strand: Direct

Name: cysU [H]

Synonym: Rru_A3401

Alternate gene names: 83594730

Gene position: 3917059-3917910 (Clockwise)

Preceding gene: 83594729

Following gene: 83594731

Centisome position: 89.99

GC content: 68.43

Gene sequence:

>852_bases
GTGACGCGATCGATCGGCCCCAAGCTGTTGGGCGGTCCCAGGGCCATTCCCGGTTTCGGCATGACCCTGGGGTTCACACT
GGCCTACCTTGGCCTTCTGGTGCTGCTGCCGTTGGCGGCCGTGGTGATCAAGGCCGCCAGCCTGGGGCCGGCCGATTTCT
GGGCGATCGCCACCGCGCCGCGCACCCTCGCCGCCTTGCGGTTGAGCTTCGGCGCGGCCTTGCTGGCGGCGGCGATCAAC
ATGGTCTTCGGCCTGCTCGTCGCCTGGGTGCTGGTGCGCTATGATTTTCCCGGCCGGCGCATTCTTGACGCCCTGGTCGA
TCTGCCCTTCGCCCTGCCCACCGCCGTGGCCGGCATCGCCCTGACCGGGCTTTACGCCAACAACGGCTGGATTGGCCAAT
ACTTGGAGCCCCTGGGCCTCAAGGTCGCCTTCGCCCCGGCCGGGGTGGTGGTGGCGCTGACCTTCGTCGGCCTGCCCTTT
GTCGTGCGCACCGTTCAGCCGGTTCTGGCCGATCTGGAGCGCGAGGCCGAGGAGGTGGCCGCCACCCTGGGGGCGGGCCG
CTGGGACACCTTTCGCCGGGTGCTGATGCCGGCCCTGGCGCCGGCCTTGCTGACCGGCTTCGCCCTGTCCTTCGCCCGCG
GTGTCGGCGAATACGGCTCGGTGATCTTCATCGCCGGCAATATTCCGATGGTCTCGGAAATCGCCCCGCTGCTGATCGTC
ATCAAGCTCGAGGAATTCGACTACCGGGGGGCCACGGCGGTGGCGGCGGTGATGCTGGCCCTGTCCTTCGCCCTGCTTCT
GGCGATCAATCTGCTCCAGTCCTGGAGCCGCGCACGCATGACGACGAGGTAG

Upstream 100 bases:

>100_bases
ATCGACGATCCGGTGTTCGGCGGCTGGACCAAGGCGCAGAAGGAGCATTTCGACGAGGGCGGTCTGTTCGATCAGATCTA
TCAGCCCGGTGGCTGACCCG

Downstream 100 bases:

>100_bases
GCGATGGCTGCTTCCCCTTCCTCTTCGGCCGTTCTGCCGACCGACCGTTCGGTGGCCCGCGCGGCGGTTTCCGAGCGGCC
GGTGGCGCGCGGCCTGCTGA

Product: sulfate ABC transporter permease protein CysT

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 283; Mature: 282

Protein sequence:

>283_residues
MTRSIGPKLLGGPRAIPGFGMTLGFTLAYLGLLVLLPLAAVVIKAASLGPADFWAIATAPRTLAALRLSFGAALLAAAIN
MVFGLLVAWVLVRYDFPGRRILDALVDLPFALPTAVAGIALTGLYANNGWIGQYLEPLGLKVAFAPAGVVVALTFVGLPF
VVRTVQPVLADLEREAEEVAATLGAGRWDTFRRVLMPALAPALLTGFALSFARGVGEYGSVIFIAGNIPMVSEIAPLLIV
IKLEEFDYRGATAVAAVMLALSFALLLAINLLQSWSRARMTTR

Sequences:

>Translated_283_residues
MTRSIGPKLLGGPRAIPGFGMTLGFTLAYLGLLVLLPLAAVVIKAASLGPADFWAIATAPRTLAALRLSFGAALLAAAIN
MVFGLLVAWVLVRYDFPGRRILDALVDLPFALPTAVAGIALTGLYANNGWIGQYLEPLGLKVAFAPAGVVVALTFVGLPF
VVRTVQPVLADLEREAEEVAATLGAGRWDTFRRVLMPALAPALLTGFALSFARGVGEYGSVIFIAGNIPMVSEIAPLLIV
IKLEEFDYRGATAVAAVMLALSFALLLAINLLQSWSRARMTTR
>Mature_282_residues
TRSIGPKLLGGPRAIPGFGMTLGFTLAYLGLLVLLPLAAVVIKAASLGPADFWAIATAPRTLAALRLSFGAALLAAAINM
VFGLLVAWVLVRYDFPGRRILDALVDLPFALPTAVAGIALTGLYANNGWIGQYLEPLGLKVAFAPAGVVVALTFVGLPFV
VRTVQPVLADLEREAEEVAATLGAGRWDTFRRVLMPALAPALLTGFALSFARGVGEYGSVIFIAGNIPMVSEIAPLLIVI
KLEEFDYRGATAVAAVMLALSFALLLAINLLQSWSRARMTTR

Specific function: Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG0555

COG function: function code O; ABC-type sulfate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788764, Length=261, Percent_Identity=51.3409961685824, Blast_Score=245, Evalue=2e-66,
Organism=Escherichia coli, GI87082099, Length=251, Percent_Identity=34.2629482071713, Blast_Score=146, Evalue=1e-36,
Organism=Escherichia coli, GI1786980, Length=229, Percent_Identity=34.061135371179, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1786254, Length=220, Percent_Identity=28.6363636363636, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1787369, Length=236, Percent_Identity=23.728813559322, Blast_Score=65, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR011865
- InterPro:   IPR005667 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 29845; Mature: 29714

Theoretical pI: Translated: 10.16; Mature: 10.16

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRSIGPKLLGGPRAIPGFGMTLGFTLAYLGLLVLLPLAAVVIKAASLGPADFWAIATAP
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCH
RTLAALRLSFGAALLAAAINMVFGLLVAWVLVRYDFPGRRILDALVDLPFALPTAVAGIA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHH
LTGLYANNGWIGQYLEPLGLKVAFAPAGVVVALTFVGLPFVVRTVQPVLADLEREAEEVA
HHHEECCCCCHHHHHHHHCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ATLGAGRWDTFRRVLMPALAPALLTGFALSFARGVGEYGSVIFIAGNIPMVSEIAPLLIV
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHEEE
IKLEEFDYRGATAVAAVMLALSFALLLAINLLQSWSRARMTTR
EEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TRSIGPKLLGGPRAIPGFGMTLGFTLAYLGLLVLLPLAAVVIKAASLGPADFWAIATAP
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCH
RTLAALRLSFGAALLAAAINMVFGLLVAWVLVRYDFPGRRILDALVDLPFALPTAVAGIA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHH
LTGLYANNGWIGQYLEPLGLKVAFAPAGVVVALTFVGLPFVVRTVQPVLADLEREAEEVA
HHHEECCCCCHHHHHHHHCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ATLGAGRWDTFRRVLMPALAPALLTGFALSFARGVGEYGSVIFIAGNIPMVSEIAPLLIV
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHEEE
IKLEEFDYRGATAVAAVMLALSFALLLAINLLQSWSRARMTTR
EEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2188958; 9205837; 9278503; 2188959 [H]