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The map label for this gene is cysW [H]

Identifier: 83594731

GI number: 83594731

Start: 3917914

End: 3918807

Strand: Direct

Name: cysW [H]

Synonym: Rru_A3402

Alternate gene names: 83594731

Gene position: 3917914-3918807 (Clockwise)

Preceding gene: 83594730

Following gene: 83594732

Centisome position: 90.01

GC content: 64.65

Gene sequence:

>894_bases
ATGGCTGCTTCCCCTTCCTCTTCGGCCGTTCTGCCGACCGACCGTTCGGTGGCCCGCGCGGCGGTTTCCGAGCGGCCGGT
GGCGCGCGGCCTGCTGATCCTGGCCGCCGTGCTGTTCCTGTGCCTTTTCTTGCTGCTGCCGCTGGTCGCCGTTTTCGTCG
AGGCGCTGTCCGATGGCGTCGCCACCTATTTCCGGGCGCTGACCGATCCCGACGCCATGTCGTCGATCCGCCTGACCCTG
ATGGTGGCGGCGATCTCGGTGCCGCTCAATCTGGTGTTCGGCGTGGCGGCGGCCTGGGCGGTGGCCAAGCATGATTTTCC
CGGGCGCAGCCTACTGGTCACGTTGATCGACCTGCCGTTCTCGGTCTCGCCGGTGATCTCGGGTCTGTGTTACGTGCTGG
TCTTTGGCGCCCAGGGCTGGTTCGGCCCCTGGCTGGTCGATCACGGGGTGCGCATCGTCTTCGCCGTTCCCGGCATCGTG
TTGGCCACGGTCTTCGTCACCCTGCCCTTCATCGCCCGGGAATTGATCCCCTTGATGGAAGAACAGGGCAGCGACGACGA
GGAGGCGGCGCTTTCTTTGGGCGCGACCGGCTGGCAGACTTTTATGCGCGTGACTCTGCCCAATATAAAATGGGGACTGC
TTTATGGCGTCTTGCTGTGCAACGCCCGGGCGATGGGCGAATTCGGCGCGGTTTCGGTGGTCAGCGGCCATATCCGGGGC
CTGACCAACACCATGCCTTTGCATGTTGAAATTCTTTACAACGAATACAGTTTCGCGGCCGCCTTCGCCGTGGCCTCGCT
GCTCGCCGTGCTGGCGCTCGTGACCCTCGTGCTCAAGACGGCATTGGAAAGTCGTCACGGCGCGGATCTCGCCCGCGGCT
CGGGCCGCCATTGA

Upstream 100 bases:

>100_bases
GGCGGTGGCGGCGGTGATGCTGGCCCTGTCCTTCGCCCTGCTTCTGGCGATCAATCTGCTCCAGTCCTGGAGCCGCGCAC
GCATGACGACGAGGTAGGCG

Downstream 100 bases:

>100_bases
CGAAAGGGGATCCCGCCATGCGGATCGATATCGAAAACATACGCAAGACCTTCCGCCATGGAACCGCGGCCGCCCTGGCC
GGCGTCGATCTGGCGGTGGC

Product: sulfate ABC transporter permease protein CysW

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 297; Mature: 296

Protein sequence:

>297_residues
MAASPSSSAVLPTDRSVARAAVSERPVARGLLILAAVLFLCLFLLLPLVAVFVEALSDGVATYFRALTDPDAMSSIRLTL
MVAAISVPLNLVFGVAAAWAVAKHDFPGRSLLVTLIDLPFSVSPVISGLCYVLVFGAQGWFGPWLVDHGVRIVFAVPGIV
LATVFVTLPFIARELIPLMEEQGSDDEEAALSLGATGWQTFMRVTLPNIKWGLLYGVLLCNARAMGEFGAVSVVSGHIRG
LTNTMPLHVEILYNEYSFAAAFAVASLLAVLALVTLVLKTALESRHGADLARGSGRH

Sequences:

>Translated_297_residues
MAASPSSSAVLPTDRSVARAAVSERPVARGLLILAAVLFLCLFLLLPLVAVFVEALSDGVATYFRALTDPDAMSSIRLTL
MVAAISVPLNLVFGVAAAWAVAKHDFPGRSLLVTLIDLPFSVSPVISGLCYVLVFGAQGWFGPWLVDHGVRIVFAVPGIV
LATVFVTLPFIARELIPLMEEQGSDDEEAALSLGATGWQTFMRVTLPNIKWGLLYGVLLCNARAMGEFGAVSVVSGHIRG
LTNTMPLHVEILYNEYSFAAAFAVASLLAVLALVTLVLKTALESRHGADLARGSGRH
>Mature_296_residues
AASPSSSAVLPTDRSVARAAVSERPVARGLLILAAVLFLCLFLLLPLVAVFVEALSDGVATYFRALTDPDAMSSIRLTLM
VAAISVPLNLVFGVAAAWAVAKHDFPGRSLLVTLIDLPFSVSPVISGLCYVLVFGAQGWFGPWLVDHGVRIVFAVPGIVL
ATVFVTLPFIARELIPLMEEQGSDDEEAALSLGATGWQTFMRVTLPNIKWGLLYGVLLCNARAMGEFGAVSVVSGHIRGL
TNTMPLHVEILYNEYSFAAAFAVASLLAVLALVTLVLKTALESRHGADLARGSGRH

Specific function: Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG4208

COG function: function code P; ABC-type sulfate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI87082099, Length=263, Percent_Identity=52.4714828897338, Blast_Score=286, Evalue=1e-78,
Organism=Escherichia coli, GI1788764, Length=269, Percent_Identity=35.6877323420074, Blast_Score=157, Evalue=7e-40,
Organism=Escherichia coli, GI1786980, Length=181, Percent_Identity=34.8066298342541, Blast_Score=90, Evalue=1e-19,
Organism=Escherichia coli, GI1787369, Length=220, Percent_Identity=29.5454545454545, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1787080, Length=250, Percent_Identity=24.4, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR011866
- InterPro:   IPR005667 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 31513; Mature: 31382

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAASPSSSAVLPTDRSVARAAVSERPVARGLLILAAVLFLCLFLLLPLVAVFVEALSDGV
CCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ATYFRALTDPDAMSSIRLTLMVAAISVPLNLVFGVAAAWAVAKHDFPGRSLLVTLIDLPF
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCC
SVSPVISGLCYVLVFGAQGWFGPWLVDHGVRIVFAVPGIVLATVFVTLPFIARELIPLME
CHHHHHHHHHHHHHHCCCCCCCHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHH
EQGSDDEEAALSLGATGWQTFMRVTLPNIKWGLLYGVLLCNARAMGEFGAVSVVSGHIRG
HCCCCHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCHHCCCCCHHHHHHHHHHC
LTNTMPLHVEILYNEYSFAAAFAVASLLAVLALVTLVLKTALESRHGADLARGSGRH
CCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCC
>Mature Secondary Structure 
AASPSSSAVLPTDRSVARAAVSERPVARGLLILAAVLFLCLFLLLPLVAVFVEALSDGV
CCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ATYFRALTDPDAMSSIRLTLMVAAISVPLNLVFGVAAAWAVAKHDFPGRSLLVTLIDLPF
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCC
SVSPVISGLCYVLVFGAQGWFGPWLVDHGVRIVFAVPGIVLATVFVTLPFIARELIPLME
CHHHHHHHHHHHHHHCCCCCCCHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHH
EQGSDDEEAALSLGATGWQTFMRVTLPNIKWGLLYGVLLCNARAMGEFGAVSVVSGHIRG
HCCCCHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCHHCCCCCHHHHHHHHHHC
LTNTMPLHVEILYNEYSFAAAFAVASLLAVLALVTLVLKTALESRHGADLARGSGRH
CCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1708375 [H]