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The map label for this gene is cobB [H]

Identifier: 83594688

GI number: 83594688

Start: 3871656

End: 3873038

Strand: Reverse

Name: cobB [H]

Synonym: Rru_A3359

Alternate gene names: 83594688

Gene position: 3873038-3871656 (Counterclockwise)

Preceding gene: 83594689

Following gene: 83594685

Centisome position: 88.98

GC content: 71.95

Gene sequence:

>1383_bases
ATGACCCTCCCTTTCGCCGCGCCTCCGCCGATCGCCCCCGGACTGATCCTGTCGGCCCCGGCTTCGGGATCGGGCAAGAC
AGTGCTTACCTTGGCGATCCTGCGCGCCCTGCGCCAGCGCGGCGTGGCGGTGGCCGGGATCAAGACCGGCCCCGATTACA
TCGACCCCGCCTTCCATGCCGCCGCTTCGGGGCGGCCTTGCGTCAATCTTGATAGCTGGGCGATGGCGCCCTCCCTACTG
CGCGGCCTTGCCCGCGCCCAGGGGACGGGGGCCGATCTGCTGCTGGCCGAAGGGGTGATGGGGTTGTTCGACGGCGCGCT
GGGCCTGCCGGCCGGGTGCGACACCGCCGATGGATCGACCGCCGCCCTGGCCCGGCTGCTGGGCTGGCCGGTGGTGCTGA
TCGTCGATGTCAAAGGCATGGCGACCAGCGTCGCCGCCCTGATCGCCGGCTTCGTCCGCCATCGCCCCGACGTGGCGGTG
CGCGGCGTCATCCTCAACCGCGTCGGCTCGGCCCGCCACGTGGCGGCGATGACCGCCGCCATCGCGAGCGAGCTTCCCGA
GCTGTGTATTCTGGGCGGCCTGCCGCGACAGGGCGATCTGGCTTTGCCCGAACGCCACCTTGGGCTGGTCCAGGCCCGCG
AGCATCCCGACCTGGAGCGTTTTCTTGACGCCAGCGCCCGGCTGCTGACCGAGCATATCGACCTTGATCGCCTGATCGCC
CTGGCCCAACCGCTCAAAAACGGCCCGACCGATGCGGCCCCCCGGCCCTTGCCGCCGCCCGGCCAGCGCATCGCCCTGGC
CCGCGACGACGCCTTCGCCTTCACCTATCCGGCGGTTCTGCAGGGTTGGCGGGACGCCGGGGCGGAGATCACTTTTTTCT
CGCCCCTGGCCGACGAGGGCCCCGACGCCACCGCCGACGCCATCCATTTGCCCGGCGGCTATCCCGAGCTTCATGCCGGG
CGTCTGGCCGCCGGTCGGACTTTCCTGCCCAGCCTGCGCGCCGCCGCCGGGCGGGGCGTGGCGATCTTTGGCGAATGCGG
CGGCTATATGGTTCTGGGGCGCGGCCTGACCGCCGCCGACGGCACCCTCCACGCCATGGCCGGGCTGCTGCCCGTCGATA
CCAGCTTCGCCGCCCGCCGCCGCCATCTTGGCTATCGGCGGGCGCGGCAGCTGTCGGCGGGCTGGCCGGGGGCGGTGGGA
GAGGGGTTTTCCGCCCATGAATTCCACTACGCCTCGATCATCGCCGAGGCGGGGGACGAGGCGGGGCGGTTGTTCGCCGT
TACGGATGCCGGCGGAACGGCGCTGGGGTCGGTCGGCCAGCGCCGGGGATCGGTGGCCGGCAGTTTTCTACATCTGATAA
GCGCGGCGCCGGGGCCAGCCTGA

Upstream 100 bases:

>100_bases
TGATGGGGCCCGATGGCCGCTTCATCACCCATTTCTCCCACGGCACTCCGTCCGATGAGATGGCGGCGCGGCTGAGCAAG
ATCCTGGCCGATACCCCCTC

Downstream 100 bases:

>100_bases
ACCCCTCGATCGCCAGCCCCTCGTGGCGGAGCAGCCAGGCCTTGCGGGCAAGGCCGCCGGCATAGCCGGTCAGGGCGCCC
GAGCCGCCGATTACCCGGTG

Product: cobyrinic acid a,c-diamide synthase

Products: ADP; phosphate; Hydrogenobyrinic acid a,c diamide; L-glutamate

Alternate protein names: NA

Number of amino acids: Translated: 460; Mature: 459

Protein sequence:

>460_residues
MTLPFAAPPPIAPGLILSAPASGSGKTVLTLAILRALRQRGVAVAGIKTGPDYIDPAFHAAASGRPCVNLDSWAMAPSLL
RGLARAQGTGADLLLAEGVMGLFDGALGLPAGCDTADGSTAALARLLGWPVVLIVDVKGMATSVAALIAGFVRHRPDVAV
RGVILNRVGSARHVAAMTAAIASELPELCILGGLPRQGDLALPERHLGLVQAREHPDLERFLDASARLLTEHIDLDRLIA
LAQPLKNGPTDAAPRPLPPPGQRIALARDDAFAFTYPAVLQGWRDAGAEITFFSPLADEGPDATADAIHLPGGYPELHAG
RLAAGRTFLPSLRAAAGRGVAIFGECGGYMVLGRGLTAADGTLHAMAGLLPVDTSFAARRRHLGYRRARQLSAGWPGAVG
EGFSAHEFHYASIIAEAGDEAGRLFAVTDAGGTALGSVGQRRGSVAGSFLHLISAAPGPA

Sequences:

>Translated_460_residues
MTLPFAAPPPIAPGLILSAPASGSGKTVLTLAILRALRQRGVAVAGIKTGPDYIDPAFHAAASGRPCVNLDSWAMAPSLL
RGLARAQGTGADLLLAEGVMGLFDGALGLPAGCDTADGSTAALARLLGWPVVLIVDVKGMATSVAALIAGFVRHRPDVAV
RGVILNRVGSARHVAAMTAAIASELPELCILGGLPRQGDLALPERHLGLVQAREHPDLERFLDASARLLTEHIDLDRLIA
LAQPLKNGPTDAAPRPLPPPGQRIALARDDAFAFTYPAVLQGWRDAGAEITFFSPLADEGPDATADAIHLPGGYPELHAG
RLAAGRTFLPSLRAAAGRGVAIFGECGGYMVLGRGLTAADGTLHAMAGLLPVDTSFAARRRHLGYRRARQLSAGWPGAVG
EGFSAHEFHYASIIAEAGDEAGRLFAVTDAGGTALGSVGQRRGSVAGSFLHLISAAPGPA
>Mature_459_residues
TLPFAAPPPIAPGLILSAPASGSGKTVLTLAILRALRQRGVAVAGIKTGPDYIDPAFHAAASGRPCVNLDSWAMAPSLLR
GLARAQGTGADLLLAEGVMGLFDGALGLPAGCDTADGSTAALARLLGWPVVLIVDVKGMATSVAALIAGFVRHRPDVAVR
GVILNRVGSARHVAAMTAAIASELPELCILGGLPRQGDLALPERHLGLVQAREHPDLERFLDASARLLTEHIDLDRLIAL
AQPLKNGPTDAAPRPLPPPGQRIALARDDAFAFTYPAVLQGWRDAGAEITFFSPLADEGPDATADAIHLPGGYPELHAGR
LAAGRTFLPSLRAAAGRGVAIFGECGGYMVLGRGLTAADGTLHAMAGLLPVDTSFAARRRHLGYRRARQLSAGWPGAVGE
GFSAHEFHYASIIAEAGDEAGRLFAVTDAGGTALGSVGQRRGSVAGSFLHLISAAPGPA

Specific function: Responsible for the amidation of carboxylic groups at position A and C of either cobyrinic acid or hydrogenobrynic acid. NH(2) groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation [H]

COG id: COG1797

COG function: function code H; Cobyrinic acid a,c-diamide synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GATase cobBQ-type domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002586
- InterPro:   IPR004484
- InterPro:   IPR017929
- InterPro:   IPR011698 [H]

Pfam domain/function: PF01656 CbiA; PF07685 GATase_3 [H]

EC number: 6.3.5.9

Molecular weight: Translated: 47265; Mature: 47134

Theoretical pI: Translated: 7.73; Mature: 7.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLPFAAPPPIAPGLILSAPASGSGKTVLTLAILRALRQRGVAVAGIKTGPDYIDPAFHA
CCCCCCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHH
AASGRPCVNLDSWAMAPSLLRGLARAQGTGADLLLAEGVMGLFDGALGLPAGCDTADGST
HCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHH
AALARLLGWPVVLIVDVKGMATSVAALIAGFVRHRPDVAVRGVILNRVGSARHVAAMTAA
HHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHH
IASELPELCILGGLPRQGDLALPERHLGLVQAREHPDLERFLDASARLLTEHIDLDRLIA
HHHHCCHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCHHHHHH
LAQPLKNGPTDAAPRPLPPPGQRIALARDDAFAFTYPAVLQGWRDAGAEITFFSPLADEG
HHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCEEECHHHHHHHHHCCCEEEEECCCCCCC
PDATADAIHLPGGYPELHAGRLAAGRTFLPSLRAAAGRGVAIFGECGGYMVLGRGLTAAD
CCCCCCEEECCCCCCCCCCCCHHCCCHHHHHHHHHCCCCEEEEECCCCEEEEECCCCCCC
GTLHAMAGLLPVDTSFAARRRHLGYRRARQLSAGWPGAVGEGFSAHEFHYASIIAEAGDE
CHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCC
AGRLFAVTDAGGTALGSVGQRRGSVAGSFLHLISAAPGPA
CCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
TLPFAAPPPIAPGLILSAPASGSGKTVLTLAILRALRQRGVAVAGIKTGPDYIDPAFHA
CCCCCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHH
AASGRPCVNLDSWAMAPSLLRGLARAQGTGADLLLAEGVMGLFDGALGLPAGCDTADGST
HCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHH
AALARLLGWPVVLIVDVKGMATSVAALIAGFVRHRPDVAVRGVILNRVGSARHVAAMTAA
HHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHH
IASELPELCILGGLPRQGDLALPERHLGLVQAREHPDLERFLDASARLLTEHIDLDRLIA
HHHHCCHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCHHHHHH
LAQPLKNGPTDAAPRPLPPPGQRIALARDDAFAFTYPAVLQGWRDAGAEITFFSPLADEG
HHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCEEECHHHHHHHHHCCCEEEEECCCCCCC
PDATADAIHLPGGYPELHAGRLAAGRTFLPSLRAAAGRGVAIFGECGGYMVLGRGLTAAD
CCCCCCEEECCCCCCCCCCCCHHCCCHHHHHHHHHCCCCEEEEECCCCEEEEECCCCCCC
GTLHAMAGLLPVDTSFAARRRHLGYRRARQLSAGWPGAVGEGFSAHEFHYASIIAEAGDE
CHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCC
AGRLFAVTDAGGTALGSVGQRRGSVAGSFLHLISAAPGPA
CCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; hydrogenobyrinic acid; L-glutamine; H2O

Specific reaction: 2 ATP + hydrogenobyrinic acid + 2 L-glutamine + 2 H2O = 2 ADP + 2 phosphate + hydrogenobyrinic acid a,c-diamide + 2 L-glutamate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2211520 [H]