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The map label for this gene is 83594685

Identifier: 83594685

GI number: 83594685

Start: 3868216

End: 3869472

Strand: Reverse

Name: 83594685

Synonym: Rru_A3356

Alternate gene names: NA

Gene position: 3869472-3868216 (Counterclockwise)

Preceding gene: 83594688

Following gene: 83594684

Centisome position: 88.9

GC content: 63.01

Gene sequence:

>1257_bases
ATGCTCTATAATCTTCATGAAATCCAGCATGCCGCGCTGATGCCCTGGCGCGTGGCCGCACACTCGGTCCATACCCTTTA
CACCTGTCCGTGGGTGCCGGCCTCTTATACCAAGATGGGGCGCTCGATCGCCGCCGGGGCGGCCGTTTTCGAGCGGGTGA
CGCGGCGCTATGGCAAGCCGCGCTTCGGTCTCGATCACACCGTCGTGGCCGGCGAAAGGGTGTGGGTCGAGGAGATCACC
GTCCACGACCTGCCGTTCTGCGATCTGGTCCATTTCACGCGCGGCTGCCCCGAGTCGGTCAAACAGCCCCGCCTGCTTGT
CGTCGCCCCGCTTTCGGGCCATTACGCCACCTTGCTGCGTGGCACGGTCGAGGCCTTGCTGCCCGATCACGATGTTTATA
TCACCGATTGGATCAACGCCCGCGAGGTGCCGATCGAAGAGGGCGCCTTCGGCTTTGACACCTATGTCGACTACCTGCGG
GCCTTCCTCACCTTCCTCGGCCCCGATACCCACCTGTTGGCGGTTTGCCAACCCAGCGTGCCGGTGCTGACCACGGCGGC
GCTGATGGCCGAGGACGGCGACGCCTGCCGGCCGCTGTCGATGACGCTGATGGGCGGCCCGCTCGATACCCGGATCAATC
CGACCGAGGTCAACCGCTTCGCCGACAGCCACGACATGGCATGGTTCGAAAAGAAGCTGATCCACACGGTTCCCGCCCCC
TATCTGGGGCGTGGCCGTAAGGTCTATCCGGGATTTCTGCAGCTTTCGGGCTTCATGGCGATGAACTTCGATCGCCATGT
GAACGCCCATCTCGACTACTTCACCCATTTGATCGAGGGCGATGGCGACAGCGCCGACCAGCATCGGGATTTCTACGACG
AATATCTGTCGGTCATGGATCTGCCGGCGGAATTCTACCTTGAGACGGTCGAGCGCATCTTCAAGAACCACGAATTGGCC
GAGGGCAAGCTGGTGGTGAACGGCCGGCGGGTGGATACGGGGGCGATTACCGATATCAGGCTCTTGACCATCGAGGGGGA
ACGCGACGATATCACCGGCAAGGGCCAGACCGCAGCGGCCCACACTTTATGCGCCAATCTGCCCCAGGCCTTGCGTGACC
ATCGCCTTCAGCCCAAGGTGGGGCATTATGGCATCTTCAACGGGCGCCGTTGGCGCGAGGAGATCCTCCCCCATGTCCGT
GCGTTCATTCTTGAAGCGGAGCGCGGCGGTGCCAGCCGCGGCGCCGCTGTCGCCTGA

Upstream 100 bases:

>100_bases
TAAGCCATAACATATCCATACAATGTATCGACTGGGAACGAATTTTTATGACCCCTTGACGATCATAAACGCGGAACGCG
GCTAACGAAAAGGACGAGGA

Downstream 100 bases:

>100_bases
CCCGTCGCTCCCGGCCTCGGAGCGGTTTTGGGAAACCAAGACGCTGAAGGAGATGACCGAGGCCGAATGGGAGAGTCTGT
GCGACGGATGCGGCAAGTGC

Product: polyhydroxyalkanoate depolymerase

Products: NA

Alternate protein names: Intracellular PHB Depolymerase; Polyhydroxyalkanoate Depolymerase; Poly-Beta-Hydroxyalkanoate Depolymerase; PHB Depolymerase; PHB De-Polymerase Domain-Containing Protein; Poly[D(-)-3-Hydroxybutyrate] Depolymerase; Intracellular Poly(3-Hydroxybutyrate) Depolymerase; Intracellular Polyhydroxyalkanoate Depolymerase; Polyhydroxyalkanoate Depolymerase Intracellular Subfamily; Poly(3-Hydroxyalkanoate) Depolymerase; Poly(3-Hydroxybutyrate) Depolymerase; Poly(3-Hydroxyalkanoate)Synthetase; Alpha/Beta Hydrolase Fold; Alpha/Beta Hydrolase Fold Protein; Intracellular PHB Depolymerase Protein; Poly-Beta-Hydroxyalkanoate Depolymerase-Like Protein; PHB De-Polymerase Domain Protein; PHB De-Polymerase; Intracellular Poly[D(-)-3-Hydroxybutyrate] Depolymerase; Poly[D(-)-3-Hydroxybutyrate](Phb) Depolymerase; Poly-Beta-Hydroxyalkanoate/Butyrate Depolymerase; Poly-Beta-Hydroxyalkanoate Depolymerase Protein; Poly[D(-)-3-Hydroxyalkanoate] Depolymerase; Polyhydroxybutanoate Depolymerase; Poly-Beta-Hydroxybutyrate Depolymerase

Number of amino acids: Translated: 418; Mature: 418

Protein sequence:

>418_residues
MLYNLHEIQHAALMPWRVAAHSVHTLYTCPWVPASYTKMGRSIAAGAAVFERVTRRYGKPRFGLDHTVVAGERVWVEEIT
VHDLPFCDLVHFTRGCPESVKQPRLLVVAPLSGHYATLLRGTVEALLPDHDVYITDWINAREVPIEEGAFGFDTYVDYLR
AFLTFLGPDTHLLAVCQPSVPVLTTAALMAEDGDACRPLSMTLMGGPLDTRINPTEVNRFADSHDMAWFEKKLIHTVPAP
YLGRGRKVYPGFLQLSGFMAMNFDRHVNAHLDYFTHLIEGDGDSADQHRDFYDEYLSVMDLPAEFYLETVERIFKNHELA
EGKLVVNGRRVDTGAITDIRLLTIEGERDDITGKGQTAAAHTLCANLPQALRDHRLQPKVGHYGIFNGRRWREEILPHVR
AFILEAERGGASRGAAVA

Sequences:

>Translated_418_residues
MLYNLHEIQHAALMPWRVAAHSVHTLYTCPWVPASYTKMGRSIAAGAAVFERVTRRYGKPRFGLDHTVVAGERVWVEEIT
VHDLPFCDLVHFTRGCPESVKQPRLLVVAPLSGHYATLLRGTVEALLPDHDVYITDWINAREVPIEEGAFGFDTYVDYLR
AFLTFLGPDTHLLAVCQPSVPVLTTAALMAEDGDACRPLSMTLMGGPLDTRINPTEVNRFADSHDMAWFEKKLIHTVPAP
YLGRGRKVYPGFLQLSGFMAMNFDRHVNAHLDYFTHLIEGDGDSADQHRDFYDEYLSVMDLPAEFYLETVERIFKNHELA
EGKLVVNGRRVDTGAITDIRLLTIEGERDDITGKGQTAAAHTLCANLPQALRDHRLQPKVGHYGIFNGRRWREEILPHVR
AFILEAERGGASRGAAVA
>Mature_418_residues
MLYNLHEIQHAALMPWRVAAHSVHTLYTCPWVPASYTKMGRSIAAGAAVFERVTRRYGKPRFGLDHTVVAGERVWVEEIT
VHDLPFCDLVHFTRGCPESVKQPRLLVVAPLSGHYATLLRGTVEALLPDHDVYITDWINAREVPIEEGAFGFDTYVDYLR
AFLTFLGPDTHLLAVCQPSVPVLTTAALMAEDGDACRPLSMTLMGGPLDTRINPTEVNRFADSHDMAWFEKKLIHTVPAP
YLGRGRKVYPGFLQLSGFMAMNFDRHVNAHLDYFTHLIEGDGDSADQHRDFYDEYLSVMDLPAEFYLETVERIFKNHELA
EGKLVVNGRRVDTGAITDIRLLTIEGERDDITGKGQTAAAHTLCANLPQALRDHRLQPKVGHYGIFNGRRWREEILPHVR
AFILEAERGGASRGAAVA

Specific function: Unknown

COG id: COG4553

COG function: function code I; Poly-beta-hydroxyalkanoate depolymerase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 46910; Mature: 46910

Theoretical pI: Translated: 6.47; Mature: 6.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLYNLHEIQHAALMPWRVAAHSVHTLYTCPWVPASYTKMGRSIAAGAAVFERVTRRYGKP
CCCCHHHHHHHHCCCHHHHHHCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
RFGLDHTVVAGERVWVEEITVHDLPFCDLVHFTRGCPESVKQPRLLVVAPLSGHYATLLR
CCCCCCEEEECCEEEEEEEEECCCCHHHHHHHHCCCCHHHCCCEEEEEECCCCCHHHHHH
GTVEALLPDHDVYITDWINAREVPIEEGAFGFDTYVDYLRAFLTFLGPDTHLLAVCQPSV
HHHHHHCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCC
PVLTTAALMAEDGDACRPLSMTLMGGPLDTRINPTEVNRFADSHDMAWFEKKLIHTVPAP
CHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHCCCC
YLGRGRKVYPGFLQLSGFMAMNFDRHVNAHLDYFTHLIEGDGDSADQHRDFYDEYLSVMD
HHCCCCEECCCHHHCCCEEEECCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHC
LPAEFYLETVERIFKNHELAEGKLVVNGRRVDTGAITDIRLLTIEGERDDITGKGQTAAA
CCHHHHHHHHHHHHCCCCCCCCEEEEECEEECCCCCCEEEEEEEECCCCCCCCCCCHHHH
HTLCANLPQALRDHRLQPKVGHYGIFNGRRWREEILPHVRAFILEAERGGASRGAAVA
HHHHHHHHHHHHHCCCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MLYNLHEIQHAALMPWRVAAHSVHTLYTCPWVPASYTKMGRSIAAGAAVFERVTRRYGKP
CCCCHHHHHHHHCCCHHHHHHCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
RFGLDHTVVAGERVWVEEITVHDLPFCDLVHFTRGCPESVKQPRLLVVAPLSGHYATLLR
CCCCCCEEEECCEEEEEEEEECCCCHHHHHHHHCCCCHHHCCCEEEEEECCCCCHHHHHH
GTVEALLPDHDVYITDWINAREVPIEEGAFGFDTYVDYLRAFLTFLGPDTHLLAVCQPSV
HHHHHHCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCC
PVLTTAALMAEDGDACRPLSMTLMGGPLDTRINPTEVNRFADSHDMAWFEKKLIHTVPAP
CHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHCCCC
YLGRGRKVYPGFLQLSGFMAMNFDRHVNAHLDYFTHLIEGDGDSADQHRDFYDEYLSVMD
HHCCCCEECCCHHHCCCEEEECCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHC
LPAEFYLETVERIFKNHELAEGKLVVNGRRVDTGAITDIRLLTIEGERDDITGKGQTAAA
CCHHHHHHHHHHHHCCCCCCCCEEEEECEEECCCCCCEEEEEEEECCCCCCCCCCCHHHH
HTLCANLPQALRDHRLQPKVGHYGIFNGRRWREEILPHVRAFILEAERGGASRGAAVA
HHHHHHHHHHHHHCCCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA