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The map label for this gene is panB

Identifier: 83594650

GI number: 83594650

Start: 3830944

End: 3831780

Strand: Reverse

Name: panB

Synonym: Rru_A3321

Alternate gene names: 83594650

Gene position: 3831780-3830944 (Counterclockwise)

Preceding gene: 83594651

Following gene: 83594649

Centisome position: 88.03

GC content: 67.5

Gene sequence:

>837_bases
ATGAGTGTCGTCCAGCCTGCCGGCCGACGTCTGACCGTTCGCGACATCGCCAGTCGCAAATCCCCCGCCGCCGAGCCGGT
GGTCTGCCTTACCGCCTATACGGCGCCGATGGCCCGTCTGCTCGACCCCCATGTCGACCTGCTGCTGGTTGGCGATTCCC
TTGGCATGGTCATCTATGGCTTGCCGACCACCCATGGCGTGACCGTCGAGATGATGATCGCCCATGGTCAGGCCGTGGTG
CGGGGCTCGTCCCATGCCCTGGTGGTGGTCGATATGCCCTTTGGTAGCTATCAGGAAAGCCCGGCCCAGGCCTTCCGCAA
CGCCGCGCGCATCCTCAGCGAGACCGGCGCCGCCGCCGTCAAGCTTGAAGGCGGCCGCGAGATGGCCGAAACCGTCGCCT
TCCTGGTGCAGCGCGGCGTGCCGGTGATGGGCCATGTCGGTCTCAAGCCGCAGATGGTCCATGCGATGGGCGGGTTCCGC
GCCCAGGGCCGCGACGAGGCCGAGGCCGAGGCGGTGGTCGCCGAAACCCGGGCCATCGCCGAGGCCGGGGCCTTCTCGAT
CGTCGTCGAAGGCACCTTTGAGCAGGTGGCGGCGCGGGCGACTCGCGAGGTTTCCGTGCCGACGATCGGCATCGGCGCCT
CGGCCGATTGCGATGGCCAGGTTCTGGTGATCGACGACGCGCTCGGTTTGTTCACCGATTTCACCCCCAAATTCGTCAAG
CGCTATGCCGATCTGGCGAGCACGGTTTCGGCGGCCGCCGAAGCCTATGCCGCCGATGTCAGGGCCCGGCGCTTTCCCGC
CCCCGAGCATTGCTTTGGCATCCGCAAGGTCGGCTGA

Upstream 100 bases:

>100_bases
GCGCTACAAGACTTCCGCCGGGCCGGCGTCTCCTGGAGGGGATCGCCGTTCCCCGCCCGGCCATCGGGTCCATTTTTGAC
CGCAAGGGAGGGCCTTCCCC

Downstream 100 bases:

>100_bases
GTTCAGGAAGCCTTCATCGATGATCTCCGATTCTCTGGAGGGCGTGCGCGCCCTGCCCATCGCCCGCTCGGTCAGCGAGT
TGCGCGCCGTCGTCGACGGT

Product: 3-methyl-2-oxobutanoate hydroxymethyltransferase

Products: NA

Alternate protein names: Ketopantoate hydroxymethyltransferase; KPHMT

Number of amino acids: Translated: 278; Mature: 277

Protein sequence:

>278_residues
MSVVQPAGRRLTVRDIASRKSPAAEPVVCLTAYTAPMARLLDPHVDLLLVGDSLGMVIYGLPTTHGVTVEMMIAHGQAVV
RGSSHALVVVDMPFGSYQESPAQAFRNAARILSETGAAAVKLEGGREMAETVAFLVQRGVPVMGHVGLKPQMVHAMGGFR
AQGRDEAEAEAVVAETRAIAEAGAFSIVVEGTFEQVAARATREVSVPTIGIGASADCDGQVLVIDDALGLFTDFTPKFVK
RYADLASTVSAAAEAYAADVRARRFPAPEHCFGIRKVG

Sequences:

>Translated_278_residues
MSVVQPAGRRLTVRDIASRKSPAAEPVVCLTAYTAPMARLLDPHVDLLLVGDSLGMVIYGLPTTHGVTVEMMIAHGQAVV
RGSSHALVVVDMPFGSYQESPAQAFRNAARILSETGAAAVKLEGGREMAETVAFLVQRGVPVMGHVGLKPQMVHAMGGFR
AQGRDEAEAEAVVAETRAIAEAGAFSIVVEGTFEQVAARATREVSVPTIGIGASADCDGQVLVIDDALGLFTDFTPKFVK
RYADLASTVSAAAEAYAADVRARRFPAPEHCFGIRKVG
>Mature_277_residues
SVVQPAGRRLTVRDIASRKSPAAEPVVCLTAYTAPMARLLDPHVDLLLVGDSLGMVIYGLPTTHGVTVEMMIAHGQAVVR
GSSHALVVVDMPFGSYQESPAQAFRNAARILSETGAAAVKLEGGREMAETVAFLVQRGVPVMGHVGLKPQMVHAMGGFRA
QGRDEAEAEAVVAETRAIAEAGAFSIVVEGTFEQVAARATREVSVPTIGIGASADCDGQVLVIDDALGLFTDFTPKFVKR
YADLASTVSAAAEAYAADVRARRFPAPEHCFGIRKVG

Specific function: Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is tranferred onto alpha-ketoisovalerate to form ketopantoate

COG id: COG0413

COG function: function code H; Ketopantoate hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the panB family

Homologues:

Organism=Escherichia coli, GI1786326, Length=244, Percent_Identity=40.5737704918033, Blast_Score=178, Evalue=4e-46,
Organism=Saccharomyces cerevisiae, GI6319653, Length=274, Percent_Identity=35.4014598540146, Blast_Score=162, Evalue=6e-41,

Paralogues:

None

Copy number: 1080 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PANB_RHORT (Q2RP30)

Other databases:

- EMBL:   CP000230
- RefSeq:   YP_428402.1
- ProteinModelPortal:   Q2RP30
- SMR:   Q2RP30
- STRING:   Q2RP30
- GeneID:   3836773
- GenomeReviews:   CP000230_GR
- KEGG:   rru:Rru_A3321
- NMPDR:   fig|1085.1.peg.2174
- eggNOG:   COG0413
- HOGENOM:   HBG299908
- OMA:   DMMIAHG
- ProtClustDB:   PRK00311
- BioCyc:   RRUB269796:RRU_A3321-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00156
- InterPro:   IPR003700
- InterPro:   IPR015813
- Gene3D:   G3DSA:3.20.20.60
- PANTHER:   PTHR20881
- PIRSF:   PIRSF000388
- TIGRFAMs:   TIGR00222

Pfam domain/function: PF02548 Pantoate_transf; SSF51621 Pyrv/PenolPyrv_Kinase_cat

EC number: =2.1.2.11

Molecular weight: Translated: 29244; Mature: 29113

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: NA

Important sites: ACT_SITE 190-190 BINDING 91-91 BINDING 121-121

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVVQPAGRRLTVRDIASRKSPAAEPVVCLTAYTAPMARLLDPHVDLLLVGDSLGMVIYG
CCCCCCCCCEEEHHHHHCCCCCCCCCEEEEEEHHHHHHHHCCCCCEEEEECCCCCEEEEE
LPTTHGVTVEMMIAHGQAVVRGSSHALVVVDMPFGSYQESPAQAFRNAARILSETGAAAV
CCCCCCCEEEEEEECCEEEEECCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCEEE
KLEGGREMAETVAFLVQRGVPVMGHVGLKPQMVHAMGGFRAQGRDEAEAEAVVAETRAIA
EECCCHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHH
EAGAFSIVVEGTFEQVAARATREVSVPTIGIGASADCDGQVLVIDDALGLFTDFTPKFVK
HCCCEEEEEECCHHHHHHHHHCCCCCCEEECCCCCCCCCEEEEEECCHHHHHCCCHHHHH
RYADLASTVSAAAEAYAADVRARRFPAPEHCFGIRKVG
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCC
>Mature Secondary Structure 
SVVQPAGRRLTVRDIASRKSPAAEPVVCLTAYTAPMARLLDPHVDLLLVGDSLGMVIYG
CCCCCCCCEEEHHHHHCCCCCCCCCEEEEEEHHHHHHHHCCCCCEEEEECCCCCEEEEE
LPTTHGVTVEMMIAHGQAVVRGSSHALVVVDMPFGSYQESPAQAFRNAARILSETGAAAV
CCCCCCCEEEEEEECCEEEEECCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCEEE
KLEGGREMAETVAFLVQRGVPVMGHVGLKPQMVHAMGGFRAQGRDEAEAEAVVAETRAIA
EECCCHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHH
EAGAFSIVVEGTFEQVAARATREVSVPTIGIGASADCDGQVLVIDDALGLFTDFTPKFVK
HCCCEEEEEECCHHHHHHHHHCCCCCCEEECCCCCCCCCEEEEEECCHHHHHCCCHHHHH
RYADLASTVSAAAEAYAADVRARRFPAPEHCFGIRKVG
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA