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The map label for this gene is fixK [H]

Identifier: 83594651

GI number: 83594651

Start: 3831947

End: 3832735

Strand: Reverse

Name: fixK [H]

Synonym: Rru_A3322

Alternate gene names: 83594651

Gene position: 3832735-3831947 (Counterclockwise)

Preceding gene: 83594652

Following gene: 83594650

Centisome position: 88.05

GC content: 65.27

Gene sequence:

>789_bases
GTGACGTTGACCAACCATGGTGCCGCCACCGCCGTTTTCTCCGGCCGCCCCTTCGACAAGCGCGGGCGGGAGGGATGTGC
GCGCGACACGCGGACCGATCCCCTCGATCCTTGTCTTCAGTGCACCCTGGGCGATCTCGCCTTTTGCAATCCCCTGCCGC
CCGACGAGTTGGAACGGCTGATGGCGATCCAAAGCAGCGTGCGGTTCGCCCCGCCCGAAACCCTGTTCAACGAGGGCGAT
CCGGCGCTTTATCTACTGTCGCCGGTTTCGGGCACCGTGAAGACCTACAAGCTGATGGCCGATGGCCGCCGCCAGATCAC
CGGATTTTTCTTCCGCGGCGATCTGTTCGGCTTTTCGGCCAATGGGGCTTACGGCTATACGGCCGAGGCGGTGACGCCGG
TGACGCTGTGCCGTTTTCCCTTGGTCAAGCTCGAGCAGCTTTTCCCGGGCGCCCCGGTGCTGGCGCGCTCGGTTCTTCAG
CGGACCCTGGCCAAACTGGCCAATTTCCACGAACAGATGTTGCTGCTTGGTCGCAAGAGCGCGCCCGAGAAGCTGGCCTC
CTTCCTGCTCTCGCTCTCGATGCGGGCCCAGGAGCGCGGCGATCCGGCCAGCCCGGTGCTGATCCCGATGGGGCGGGCCG
ATGTGGCCGATTATCTGGGGCTGACCATCGAAACGGTCAGCCGGACGCTGAGCAAGTTCCGCGTTCAGGGGCTGGTCGAA
CTGCCCAATCCCAGCACGATCACCCTGTGCGACCGCGGCGCCCTGCGCGCCATCGCCGACGGTCTTTGA

Upstream 100 bases:

>100_bases
TCAGATGCGGTCCAATCAAGAAAGCAGACAAAACCAAAGAGCGGGCCGTCCTTATCTTTCCTAAGGGCCGAAGCCCGATC
AGACAGGGATCGGAGAGACC

Downstream 100 bases:

>100_bases
CGCGAGGAGGGCCGCCGCCGCGCCCTTGGCGGGTGCGGCGATAGATCTTCCCCTTCCGCACGCCGCGCGCTACAAGACTT
CCGCCGGGCCGGCGTCTCCT

Product: Crp/FNR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 262; Mature: 261

Protein sequence:

>262_residues
MTLTNHGAATAVFSGRPFDKRGREGCARDTRTDPLDPCLQCTLGDLAFCNPLPPDELERLMAIQSSVRFAPPETLFNEGD
PALYLLSPVSGTVKTYKLMADGRRQITGFFFRGDLFGFSANGAYGYTAEAVTPVTLCRFPLVKLEQLFPGAPVLARSVLQ
RTLAKLANFHEQMLLLGRKSAPEKLASFLLSLSMRAQERGDPASPVLIPMGRADVADYLGLTIETVSRTLSKFRVQGLVE
LPNPSTITLCDRGALRAIADGL

Sequences:

>Translated_262_residues
MTLTNHGAATAVFSGRPFDKRGREGCARDTRTDPLDPCLQCTLGDLAFCNPLPPDELERLMAIQSSVRFAPPETLFNEGD
PALYLLSPVSGTVKTYKLMADGRRQITGFFFRGDLFGFSANGAYGYTAEAVTPVTLCRFPLVKLEQLFPGAPVLARSVLQ
RTLAKLANFHEQMLLLGRKSAPEKLASFLLSLSMRAQERGDPASPVLIPMGRADVADYLGLTIETVSRTLSKFRVQGLVE
LPNPSTITLCDRGALRAIADGL
>Mature_261_residues
TLTNHGAATAVFSGRPFDKRGREGCARDTRTDPLDPCLQCTLGDLAFCNPLPPDELERLMAIQSSVRFAPPETLFNEGDP
ALYLLSPVSGTVKTYKLMADGRRQITGFFFRGDLFGFSANGAYGYTAEAVTPVTLCRFPLVKLEQLFPGAPVLARSVLQR
TLAKLANFHEQMLLLGRKSAPEKLASFLLSLSMRAQERGDPASPVLIPMGRADVADYLGLTIETVSRTLSKFRVQGLVEL
PNPSTITLCDRGALRAIADGL

Specific function: B.japonicum has two fixLJ-dependent fixK homologs that are activators of the transcription of a group of genes involved in anaerobic processes such as denitrification and possibly nitrogen fixation. FixK may bind DNA at the FNR consensus binding site [H]

COG id: COG0664

COG function: function code T; cAMP-binding proteins - catabolite gene activator and regulatory subunit of cAMP-dependent protein kinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH crp-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787595, Length=225, Percent_Identity=32.8888888888889, Blast_Score=120, Evalue=1e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018490
- InterPro:   IPR000595
- InterPro:   IPR001808
- InterPro:   IPR012318
- InterPro:   IPR014710
- InterPro:   IPR018335
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00027 cNMP_binding; PF00325 Crp [H]

EC number: NA

Molecular weight: Translated: 28480; Mature: 28349

Theoretical pI: Translated: 8.36; Mature: 8.36

Prosite motif: PS50042 CNMP_BINDING_3 ; PS51063 HTH_CRP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLTNHGAATAVFSGRPFDKRGREGCARDTRTDPLDPCLQCTLGDLAFCNPLPPDELERL
CCCCCCCCEEEEECCCCCCHHHHHCCCCCCCCCCCCHHHHHHHCCHHHCCCCCHHHHHHH
MAIQSSVRFAPPETLFNEGDPALYLLSPVSGTVKTYKLMADGRRQITGFFFRGDLFGFSA
HHHHHHCCCCCCHHHHCCCCCEEEEECCCCCCHHHHHHHHCCCHHHEEEEEECEEEEEEC
NGAYGYTAEAVTPVTLCRFPLVKLEQLFPGAPVLARSVLQRTLAKLANFHEQMLLLGRKS
CCCCCCCCHHHCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
APEKLASFLLSLSMRAQERGDPASPVLIPMGRADVADYLGLTIETVSRTLSKFRVQGLVE
CHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCEEE
LPNPSTITLCDRGALRAIADGL
CCCCCEEEEECCCHHHHHHCCC
>Mature Secondary Structure 
TLTNHGAATAVFSGRPFDKRGREGCARDTRTDPLDPCLQCTLGDLAFCNPLPPDELERL
CCCCCCCEEEEECCCCCCHHHHHCCCCCCCCCCCCHHHHHHHCCHHHCCCCCHHHHHHH
MAIQSSVRFAPPETLFNEGDPALYLLSPVSGTVKTYKLMADGRRQITGFFFRGDLFGFSA
HHHHHHCCCCCCHHHHCCCCCEEEEECCCCCCHHHHHHHHCCCHHHEEEEEECEEEEEEC
NGAYGYTAEAVTPVTLCRFPLVKLEQLFPGAPVLARSVLQRTLAKLANFHEQMLLLGRKS
CCCCCCCCHHHCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
APEKLASFLLSLSMRAQERGDPASPVLIPMGRADVADYLGLTIETVSRTLSKFRVQGLVE
CHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCEEE
LPNPSTITLCDRGALRAIADGL
CCCCCEEEEECCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1551834; 12597275 [H]