Click here to switch to the map view.

The map label for this gene is metZ [H]

Identifier: 83594544

GI number: 83594544

Start: 3705744

End: 3706985

Strand: Reverse

Name: metZ [H]

Synonym: Rru_A3214

Alternate gene names: 83594544

Gene position: 3706985-3705744 (Counterclockwise)

Preceding gene: 83594550

Following gene: 83594542

Centisome position: 85.16

GC content: 66.1

Gene sequence:

>1242_bases
ATGCCCCGTGAAAGCGATTTTTCCCCCGCCCAGTCTCCGTCTCTTGAAGCGCCCACCGGCCCTGCGACTTGGCGGGCCGA
TACGTTACTGGTGCGCGGCGGGTTGGCTCGGACTGGTTTGAATGAAACCAGCGAAGCGCTGTTTCTCAATTCCGGCTATG
TCTATCCCAATGCCGAGGAGGCCGAGGCGGCTTTCGACGGCACGCTCGAACGCTATGTCTATTCGCGCTTCCGCAATCCG
ACGATCAGCGTTTTCGAAGAGCGACTCGCCGCCCTGGAAGGGGCGCCGGTTTGCAAGGCCACGGCCAGCGGCATGGCCGC
CGTCACCTCGGCCCTGCTCTGTCAGGTGCGGGCGGGCGATCGGGTGGTGGCGGCGCGCGATCTGTTTGGCTCGTGCTCCT
GGGTGATCGGCGATCTGCTGGCCCAATACGGAGTCAGCGCCGAATTCGTCGATACCGAGAACCTTGACGCCTGGGCCCAG
GCCCTGGCCAAGCCGACCCGGGCGGTTTTCCTGGAAACCCCGTCCAATCCGACGCTGCGCATCGTCGATCTCAAGGGGGT
TTGCGACCTCGCCCATGCCGCTGGGGCGACGGTGGTGGTCGATAACGCCTTCGCCACGCCGCTTTTGCAGCGGCCGCGCG
ACTTCGGCGCCGATGTCGTCGTCCATTCGGCGACCAAATGGATCGACGGCCAGGGCCGCTGCCTGGGCGGCGCCGTCCTC
TGCGACGAGGCGTTCAACGAGACCTATCTCGGGCCGTTCCTGCGCCATACCGGTCCCTGCCTCGCCCCGTTCAACGCCTG
GGTCATGCTCAAGGGCCTGGAAACCCTGTCGCTGCGCATCAACCGCCACAGCGCGACGGCTTTGACCCTGGCCGGGCTGA
TCGAAGGCCACCCCGCGGTCGCCCGCGCCCTTTATCCCGGGTTGGCCAGCCATCCGCGCCATGCCTTGGCGCAAAGCCAG
ATGAAGGCCGGCGGCGGCGTCATCGCCCTGTCGCTGAAGGGCGGACGGGCCAGCGCCTATCGCTTCCTCAACGCGCTGTC
GATCGTCGATATCTCGAATAATCTGGGCGACGCCAAATCGCTGGCCTGCCATCCGTGGACGACCACCCACCAGCGCCTGA
GCCCGGAAGAAAAACTGATCCAGGGCATCGATGAAGGGCTGATCCGCTTTTCCGTCGGCTTGGAAGACCCCGAGGATCTG
GCCGCCGACATCGGCGCCGCCCTCGACGCCGCCGGCGGTTGA

Upstream 100 bases:

>100_bases
GCCCACGTTAAACAAACAGCTAAAAGGGTCGTGTGACGAAAGTCCCGGCCTCGGATCGCCGGGCTTTTTTCTTTTGGACG
TCACACGAAGGAGGGCCCCT

Downstream 100 bases:

>100_bases
CGATACGGCCGTCCGATCCGCGCGGGGCGGTGGACCTTTTAGGATTCCACCGCTCCGGCGGGCAGGCGCACGGTGATGCG
GGTGCCCGCGCCCGGCGTGC

Product: O-succinylhomoserine sulfhydrylase

Products: Cystathionine; succinate; o-succinyl-L-homoserine; H2O [C]

Alternate protein names: OSH sulfhydrylase [H]

Number of amino acids: Translated: 413; Mature: 412

Protein sequence:

>413_residues
MPRESDFSPAQSPSLEAPTGPATWRADTLLVRGGLARTGLNETSEALFLNSGYVYPNAEEAEAAFDGTLERYVYSRFRNP
TISVFEERLAALEGAPVCKATASGMAAVTSALLCQVRAGDRVVAARDLFGSCSWVIGDLLAQYGVSAEFVDTENLDAWAQ
ALAKPTRAVFLETPSNPTLRIVDLKGVCDLAHAAGATVVVDNAFATPLLQRPRDFGADVVVHSATKWIDGQGRCLGGAVL
CDEAFNETYLGPFLRHTGPCLAPFNAWVMLKGLETLSLRINRHSATALTLAGLIEGHPAVARALYPGLASHPRHALAQSQ
MKAGGGVIALSLKGGRASAYRFLNALSIVDISNNLGDAKSLACHPWTTTHQRLSPEEKLIQGIDEGLIRFSVGLEDPEDL
AADIGAALDAAGG

Sequences:

>Translated_413_residues
MPRESDFSPAQSPSLEAPTGPATWRADTLLVRGGLARTGLNETSEALFLNSGYVYPNAEEAEAAFDGTLERYVYSRFRNP
TISVFEERLAALEGAPVCKATASGMAAVTSALLCQVRAGDRVVAARDLFGSCSWVIGDLLAQYGVSAEFVDTENLDAWAQ
ALAKPTRAVFLETPSNPTLRIVDLKGVCDLAHAAGATVVVDNAFATPLLQRPRDFGADVVVHSATKWIDGQGRCLGGAVL
CDEAFNETYLGPFLRHTGPCLAPFNAWVMLKGLETLSLRINRHSATALTLAGLIEGHPAVARALYPGLASHPRHALAQSQ
MKAGGGVIALSLKGGRASAYRFLNALSIVDISNNLGDAKSLACHPWTTTHQRLSPEEKLIQGIDEGLIRFSVGLEDPEDL
AADIGAALDAAGG
>Mature_412_residues
PRESDFSPAQSPSLEAPTGPATWRADTLLVRGGLARTGLNETSEALFLNSGYVYPNAEEAEAAFDGTLERYVYSRFRNPT
ISVFEERLAALEGAPVCKATASGMAAVTSALLCQVRAGDRVVAARDLFGSCSWVIGDLLAQYGVSAEFVDTENLDAWAQA
LAKPTRAVFLETPSNPTLRIVDLKGVCDLAHAAGATVVVDNAFATPLLQRPRDFGADVVVHSATKWIDGQGRCLGGAVLC
DEAFNETYLGPFLRHTGPCLAPFNAWVMLKGLETLSLRINRHSATALTLAGLIEGHPAVARALYPGLASHPRHALAQSQM
KAGGGVIALSLKGGRASAYRFLNALSIVDISNNLGDAKSLACHPWTTTHQRLSPEEKLIQGIDEGLIRFSVGLEDPEDLA
ADIGAALDAAGG

Specific function: Converts O-succinylhomoserine into homocysteine [H]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=344, Percent_Identity=35.1744186046512, Blast_Score=204, Evalue=1e-52,
Organism=Homo sapiens, GI299473759, Length=344, Percent_Identity=32.5581395348837, Blast_Score=170, Evalue=2e-42,
Organism=Homo sapiens, GI25453487, Length=343, Percent_Identity=30.3206997084548, Blast_Score=144, Evalue=1e-34,
Organism=Escherichia coli, GI1790375, Length=382, Percent_Identity=34.2931937172775, Blast_Score=208, Evalue=6e-55,
Organism=Escherichia coli, GI1789383, Length=391, Percent_Identity=24.0409207161125, Blast_Score=114, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17560056, Length=332, Percent_Identity=37.0481927710843, Blast_Score=221, Evalue=5e-58,
Organism=Caenorhabditis elegans, GI17538055, Length=359, Percent_Identity=35.3760445682451, Blast_Score=221, Evalue=7e-58,
Organism=Caenorhabditis elegans, GI71980708, Length=348, Percent_Identity=31.6091954022989, Blast_Score=186, Evalue=3e-47,
Organism=Saccharomyces cerevisiae, GI6323333, Length=431, Percent_Identity=33.1786542923434, Blast_Score=213, Evalue=5e-56,
Organism=Saccharomyces cerevisiae, GI6319307, Length=347, Percent_Identity=34.8703170028818, Blast_Score=195, Evalue=1e-50,
Organism=Saccharomyces cerevisiae, GI6321254, Length=410, Percent_Identity=27.3170731707317, Blast_Score=132, Evalue=9e-32,
Organism=Saccharomyces cerevisiae, GI6321170, Length=290, Percent_Identity=26.8965517241379, Blast_Score=110, Evalue=5e-25,
Organism=Saccharomyces cerevisiae, GI6321904, Length=342, Percent_Identity=26.9005847953216, Blast_Score=99, Evalue=1e-21,
Organism=Drosophila melanogaster, GI19922554, Length=344, Percent_Identity=35.4651162790698, Blast_Score=221, Evalue=7e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006234
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: 2.5.1.48 [C]

Molecular weight: Translated: 43708; Mature: 43577

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRESDFSPAQSPSLEAPTGPATWRADTLLVRGGLARTGLNETSEALFLNSGYVYPNAEE
CCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCHHCCCCCCCCEEEEECCCCCCCCHH
AEAAFDGTLERYVYSRFRNPTISVFEERLAALEGAPVCKATASGMAAVTSALLCQVRAGD
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCC
RVVAARDLFGSCSWVIGDLLAQYGVSAEFVDTENLDAWAQALAKPTRAVFLETPSNPTLR
EEEEHHHHHCCHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHCCCCEEEEEECCCCCCEE
IVDLKGVCDLAHAAGATVVVDNAFATPLLQRPRDFGADVVVHSATKWIDGQGRCLGGAVL
EEEECCHHHHHHHCCCEEEECCCHHCHHHHCCHHCCCCEEEECHHHEECCCCCEECCEEE
CDEAFNETYLGPFLRHTGPCLAPFNAWVMLKGLETLSLRINRHSATALTLAGLIEGHPAV
EHHHCCCHHCCHHHHHCCCCCCCCCCEEEEECCEEEEEEEECCCHHHHHHHHHHCCCHHH
ARALYPGLASHPRHALAQSQMKAGGGVIALSLKGGRASAYRFLNALSIVDISNNLGDAKS
HHHHHCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHEEEEECCCCCCCCC
LACHPWTTTHQRLSPEEKLIQGIDEGLIRFSVGLEDPEDLAADIGAALDAAGG
EEECCCCCCHHHCCHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
PRESDFSPAQSPSLEAPTGPATWRADTLLVRGGLARTGLNETSEALFLNSGYVYPNAEE
CCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCHHCCCCCCCCEEEEECCCCCCCCHH
AEAAFDGTLERYVYSRFRNPTISVFEERLAALEGAPVCKATASGMAAVTSALLCQVRAGD
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCC
RVVAARDLFGSCSWVIGDLLAQYGVSAEFVDTENLDAWAQALAKPTRAVFLETPSNPTLR
EEEEHHHHHCCHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHCCCCEEEEEECCCCCCEE
IVDLKGVCDLAHAAGATVVVDNAFATPLLQRPRDFGADVVVHSATKWIDGQGRCLGGAVL
EEEECCHHHHHHHCCCEEEECCCHHCHHHHCCHHCCCCEEEECHHHEECCCCCEECCEEE
CDEAFNETYLGPFLRHTGPCLAPFNAWVMLKGLETLSLRINRHSATALTLAGLIEGHPAV
EHHHCCCHHCCHHHHHCCCCCCCCCCEEEEECCEEEEEEEECCCHHHHHHHHHHCCCHHH
ARALYPGLASHPRHALAQSQMKAGGGVIALSLKGGRASAYRFLNALSIVDISNNLGDAKS
HHHHHCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHEEEEECCCCCCCCC
LACHPWTTTHQRLSPEEKLIQGIDEGLIRFSVGLEDPEDLAADIGAALDAAGG
EEECCCCCCHHHCCHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: pyridoxal 5'-phosphate [C]

Metal ions: NA

Kcat value (1/min): 460 [C]

Specific activity: 10

Km value (mM): 0.33 {O-succinyl-L-homoserine}} [C]

Substrates: O-succinyl-L-homoserine; @CYST01.txt*L-cysteine!; 2-oxobutanoate; succinate; NH3 [C]

Specific reaction: O-succinyl-L-homoserine + @CYST01.txt*L-cysteine! = cystathionine + succinate 2-oxobutanoate + succinate + NH3 = o-succinyl-L-homoserine + H2O [C]

General reaction: Elimination; Gamma-replacement [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7704274; 10984043 [H]