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The map label for this gene is pyrB

Identifier: 83594520

GI number: 83594520

Start: 3679306

End: 3680265

Strand: Direct

Name: pyrB

Synonym: Rru_A3190

Alternate gene names: 83594520

Gene position: 3679306-3680265 (Clockwise)

Preceding gene: 83594519

Following gene: 83594521

Centisome position: 84.53

GC content: 65.1

Gene sequence:

>960_bases
ATGAGCGCGATCTTGTTCCCCGAATACACCCACCGCCATCTGCTCGGAATCGAAGGCCTGGTGCCCGTCGAGGTCACCGC
CCTGCTCGACCGCGCCGAGATCTACGCCAACCGCAATCGCTCGGCCAACAAGGTCAGCGACGAGATGCGGGGGCGCACGG
TCATCAACCTGTTCTTCGAAAATTCGACGCGCACCCGCACCAGCTTCGAACTGGCGGCGCGGCGCCTGGGCGCCGATGTC
ATCAACATGCAGGTGGGCTCGAGCAGTGTCGCCAAGGGGGAAACCCTGATCGACACCGCCGTCACCCTCAACGCCATGCA
TCCCGATGTGCTGGTGGTCCGCCATGCCGAGTCGGGCGCGGCGGCCCTGCTCGCCCAAAAGGTCAATTGCGCGGTCATCA
ACGCCGGCGACGGCGCCCACGAGCATCCGACCCAGGCCCTGCTCGACGCCCTGACCATCCGCCGGCGCAAGGGCCGGCTG
GGCGGGCTGGACGTGGCGATCTGCGGCGATGTTCTGCATTCGCGGGTGGCGCGGTCCAACATCCACTTGCTGACCCTGAT
GGGGGCGCGGGTGCGCGTTGTCGGACCACGGCCGCTGATCCCCTCGGGCATCGGCGAATTGGGCGTCGATATCTTCCATG
ACATGCGCGAAGGCCTGCGCGGCGTTGATATCGTGATGATGCTGCGCATCCAGATGGAGCGCATGCAGGGCAACTTCATT
CCCAGCGTGCGCGAATACTTCCGCTATTTCGGCCTCGACCGCGAGAAACTGGCCCTGGCCAAGCCCGATGCCCTGATCAT
GCATCCGGGACCGATGAATCGCGGGGTCGAGATCGATTCCGACGTGGCCGATGATTTCGAACGCAGCGTGATCCGGGAGC
AGGTGGAAATGGGCGTCGCGGTGCGCATGGCGGTTCTCGAGGTGCTGTCGGGCAACCTCGCCTCGATGGAGACCGCATAA

Upstream 100 bases:

>100_bases
CGCCCGAATCTCCCCCTGTCAAAAGACCTTGGCGACGGCCCCTTGCGGGCCGTCGTTTTGATTGCCCGCGCGCGGGGCCG
GCCCTATAGTCCGGGCCATC

Downstream 100 bases:

>100_bases
TGTCGCTGCGTTCCCCCGGTCGCGTCGCCTATGTCAACGCCCGCCTGCTCGATCCGGCCAGCGGCCTGGATCAAACGGGC
GCCCTGCTGGCCGATGGCGA

Product: aspartate carbamoyltransferase

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase

Number of amino acids: Translated: 319; Mature: 318

Protein sequence:

>319_residues
MSAILFPEYTHRHLLGIEGLVPVEVTALLDRAEIYANRNRSANKVSDEMRGRTVINLFFENSTRTRTSFELAARRLGADV
INMQVGSSSVAKGETLIDTAVTLNAMHPDVLVVRHAESGAAALLAQKVNCAVINAGDGAHEHPTQALLDALTIRRRKGRL
GGLDVAICGDVLHSRVARSNIHLLTLMGARVRVVGPRPLIPSGIGELGVDIFHDMREGLRGVDIVMMLRIQMERMQGNFI
PSVREYFRYFGLDREKLALAKPDALIMHPGPMNRGVEIDSDVADDFERSVIREQVEMGVAVRMAVLEVLSGNLASMETA

Sequences:

>Translated_319_residues
MSAILFPEYTHRHLLGIEGLVPVEVTALLDRAEIYANRNRSANKVSDEMRGRTVINLFFENSTRTRTSFELAARRLGADV
INMQVGSSSVAKGETLIDTAVTLNAMHPDVLVVRHAESGAAALLAQKVNCAVINAGDGAHEHPTQALLDALTIRRRKGRL
GGLDVAICGDVLHSRVARSNIHLLTLMGARVRVVGPRPLIPSGIGELGVDIFHDMREGLRGVDIVMMLRIQMERMQGNFI
PSVREYFRYFGLDREKLALAKPDALIMHPGPMNRGVEIDSDVADDFERSVIREQVEMGVAVRMAVLEVLSGNLASMETA
>Mature_318_residues
SAILFPEYTHRHLLGIEGLVPVEVTALLDRAEIYANRNRSANKVSDEMRGRTVINLFFENSTRTRTSFELAARRLGADVI
NMQVGSSSVAKGETLIDTAVTLNAMHPDVLVVRHAESGAAALLAQKVNCAVINAGDGAHEHPTQALLDALTIRRRKGRLG
GLDVAICGDVLHSRVARSNIHLLTLMGARVRVVGPRPLIPSGIGELGVDIFHDMREGLRGVDIVMMLRIQMERMQGNFIP
SVREYFRYFGLDREKLALAKPDALIMHPGPMNRGVEIDSDVADDFERSVIREQVEMGVAVRMAVLEVLSGNLASMETA

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family

Homologues:

Organism=Homo sapiens, GI18105007, Length=317, Percent_Identity=34.7003154574132, Blast_Score=158, Evalue=7e-39,
Organism=Escherichia coli, GI2367364, Length=279, Percent_Identity=35.1254480286738, Blast_Score=132, Evalue=3e-32,
Organism=Escherichia coli, GI1790703, Length=230, Percent_Identity=26.5217391304348, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1786469, Length=227, Percent_Identity=25.9911894273128, Blast_Score=69, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI193204318, Length=335, Percent_Identity=33.4328358208955, Blast_Score=137, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6322331, Length=278, Percent_Identity=37.7697841726619, Blast_Score=151, Evalue=2e-37,
Organism=Drosophila melanogaster, GI24642586, Length=312, Percent_Identity=34.2948717948718, Blast_Score=148, Evalue=6e-36,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): PYRB_RHORT (Q2RPG0)

Other databases:

- EMBL:   CP000230
- RefSeq:   YP_428272.1
- HSSP:   P0A786
- ProteinModelPortal:   Q2RPG0
- SMR:   Q2RPG0
- STRING:   Q2RPG0
- GeneID:   3836636
- GenomeReviews:   CP000230_GR
- KEGG:   rru:Rru_A3190
- NMPDR:   fig|1085.1.peg.832
- eggNOG:   COG0540
- HOGENOM:   HBG579429
- OMA:   MNRGLEI
- BioCyc:   RRUB269796:RRU_A3190-MONOMER
- HAMAP:   MF_00001
- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082
- PRINTS:   PR00100
- PRINTS:   PR00101
- TIGRFAMs:   TIGR00670

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N; SSF53671 Asp/Orn_carbamoyltranf

EC number: =2.1.3.2

Molecular weight: Translated: 34978; Mature: 34847

Theoretical pI: Translated: 7.08; Mature: 7.08

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAILFPEYTHRHLLGIEGLVPVEVTALLDRAEIYANRNRSANKVSDEMRGRTVINLFFE
CCCEECCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCEEEEEEEE
NSTRTRTSFELAARRLGADVINMQVGSSSVAKGETLIDTAVTLNAMHPDVLVVRHAESGA
CCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHEEECCCCCEEEEEECCCCH
AALLAQKVNCAVINAGDGAHEHPTQALLDALTIRRRKGRLGGLDVAICGDVLHSRVARSN
HHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCC
IHLLTLMGARVRVVGPRPLIPSGIGELGVDIFHDMREGLRGVDIVMMLRIQMERMQGNFI
CEEEEEECCEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCC
PSVREYFRYFGLDREKLALAKPDALIMHPGPMNRGVEIDSDVADDFERSVIREQVEMGVA
HHHHHHHHHHCCCHHHHEECCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
VRMAVLEVLSGNLASMETA
HHHHHHHHHCCCCHHCCCC
>Mature Secondary Structure 
SAILFPEYTHRHLLGIEGLVPVEVTALLDRAEIYANRNRSANKVSDEMRGRTVINLFFE
CCEECCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCEEEEEEEE
NSTRTRTSFELAARRLGADVINMQVGSSSVAKGETLIDTAVTLNAMHPDVLVVRHAESGA
CCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHEEECCCCCEEEEEECCCCH
AALLAQKVNCAVINAGDGAHEHPTQALLDALTIRRRKGRLGGLDVAICGDVLHSRVARSN
HHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCC
IHLLTLMGARVRVVGPRPLIPSGIGELGVDIFHDMREGLRGVDIVMMLRIQMERMQGNFI
CEEEEEECCEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCC
PSVREYFRYFGLDREKLALAKPDALIMHPGPMNRGVEIDSDVADDFERSVIREQVEMGVA
HHHHHHHHHHCCCHHHHEECCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
VRMAVLEVLSGNLASMETA
HHHHHHHHHCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA