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The map label for this gene is gatB

Identifier: 83594505

GI number: 83594505

Start: 3666819

End: 3668276

Strand: Reverse

Name: gatB

Synonym: Rru_A3175

Alternate gene names: 83594505

Gene position: 3668276-3666819 (Counterclockwise)

Preceding gene: 83594506

Following gene: 83594503

Centisome position: 84.27

GC content: 63.65

Gene sequence:

>1458_bases
ATGACCTATCTCATCAAAGGGGAAACCGCGACCTGGGAAGTGGTTGTCGGTCTGGAAGTCCATGCCCAGGTCGTCAGCCA
GTCCAAGCTGTTCTCGGGCTCGTCCACCCGCTTCGGCGCCCCGGCCAATAGCCAAGTGTCGCTGGTCGACGCGGCGATGC
CGGGCATGTTGCCGGTGATCAATGGCCTGTGCGTTGAACAGGCGGTGCGCACCGGTCTGGGCCTGCGGGCCAAGATCAAC
AAGCGGTCGATCTTCGCGCGCAAGAACTACTTCTATGCCGACCTGCCCCAGGGCTATCAGATCAGCCAGTACGAACAGCC
GATCGTTGGCGAGGGCAAGGTGGTGCTCGACATGCCCGACGGCTCGTCGCGCGAAGTCGGCATCGAACGCCTGCATCTGG
AACAGGATGCCGGCAAGTCGATGCACGACCAGCATCCGACCAAAAGCCTGATCGACCTCAACCGCTCGGGCGTGGCGCTG
ATGGAGATCGTCTCCAAGCCCGACATGCGCGAGCCCGAGGAGGCCGGCGCCTACTTGCGCAAGCTGCGCTCGATCATGCG
CTATCTGGGAACTTGCGACGGCAACATGGAGGAAGGCTCCATGCGCGCCGACGTCAATGTGTCGGTGCGGCCCATCGGCA
CCACCGATCTGCGCACCCGCTGCGAGATCAAGAACGTCAATTCGGTGCGCTTCGTCATGCAGGCGATCGAGGCCGAGGCC
CATCGGCAGGTCGAGGTGTGGGAGAGCGGCGGCGAGGTTCGCCAGGAAACCCGCCTGTTCGATCCGGCCAAGGGCGAAAC
GCGGTCGATGCGCTCCAAGGAGCACGCCCACGACTATCGCTATTTCCCCGATCCCGACCTGCTGCCCCTGGAATTCGACG
ACGCCTTCGTCGAGAAGGTGCGCCGCAGCCTGCCCGAACTGCCCGACGACAAGAAGAACCGCTTCATCGCCGATTTCGGG
CTGACCCCCTATGACGCCAGCGTGCTGGTCGTCGATCGCGAGAACGCCGCCTTCTTTGAAAAGGTGGCCGAGGGGCGCGA
TGGCAAGACCGCGGCCAATTGGGTGATCAATAACCTGTTCGGCGCCCTGGCCAAGGCCGGCAAGGGCGTGGCCGACAGCC
CGGTTTCGGCCGAGAACCTGGGCAAGCTGCTTGATCTGCTCAAGGACGAGGTGATCAACGGCCGCATCGCCAAGGATGTC
TTCGAGATCATGATGGAGACCGGCGAGGACCCCGATGCCATCGTCGAGGACAAGGGCCTGCGTCAGGTCACCGATACCAG
CGCCATCGAGGCGGCGGTCGACGCGGTGATCGCGGCCAATCCCGAAAAGGCCGATGACGTGCGCGGCGGCAAGGAAAAGC
TGCTGGGCTGGTTCGTTGGTCAGGTGATGAAGGCGACGCAGGGCAAGGCCAATCCGGCCATGCTCAACGACATCCTGCGC
GCGAAACTTCTGGGCTGA

Upstream 100 bases:

>100_bases
ACGAGGAAACCGTGCTCAACGCCGGTCTGGCCCTGGAACGGGCCAGCGCCTTCACCCAGAAGCCAGCGTTCCTGACCGGC
GCTTTGGAAGGGCAGATCTG

Downstream 100 bases:

>100_bases
CCGGCCCCGCTTCTTTCCCGCCCGCCCCACAGGATCGGGCGGGAAAGACCAGCGCCCCGGCCAGGGCCAATAGGCTGAGG
GCGACGACCAGCCCGCCATA

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B

Number of amino acids: Translated: 485; Mature: 484

Protein sequence:

>485_residues
MTYLIKGETATWEVVVGLEVHAQVVSQSKLFSGSSTRFGAPANSQVSLVDAAMPGMLPVINGLCVEQAVRTGLGLRAKIN
KRSIFARKNYFYADLPQGYQISQYEQPIVGEGKVVLDMPDGSSREVGIERLHLEQDAGKSMHDQHPTKSLIDLNRSGVAL
MEIVSKPDMREPEEAGAYLRKLRSIMRYLGTCDGNMEEGSMRADVNVSVRPIGTTDLRTRCEIKNVNSVRFVMQAIEAEA
HRQVEVWESGGEVRQETRLFDPAKGETRSMRSKEHAHDYRYFPDPDLLPLEFDDAFVEKVRRSLPELPDDKKNRFIADFG
LTPYDASVLVVDRENAAFFEKVAEGRDGKTAANWVINNLFGALAKAGKGVADSPVSAENLGKLLDLLKDEVINGRIAKDV
FEIMMETGEDPDAIVEDKGLRQVTDTSAIEAAVDAVIAANPEKADDVRGGKEKLLGWFVGQVMKATQGKANPAMLNDILR
AKLLG

Sequences:

>Translated_485_residues
MTYLIKGETATWEVVVGLEVHAQVVSQSKLFSGSSTRFGAPANSQVSLVDAAMPGMLPVINGLCVEQAVRTGLGLRAKIN
KRSIFARKNYFYADLPQGYQISQYEQPIVGEGKVVLDMPDGSSREVGIERLHLEQDAGKSMHDQHPTKSLIDLNRSGVAL
MEIVSKPDMREPEEAGAYLRKLRSIMRYLGTCDGNMEEGSMRADVNVSVRPIGTTDLRTRCEIKNVNSVRFVMQAIEAEA
HRQVEVWESGGEVRQETRLFDPAKGETRSMRSKEHAHDYRYFPDPDLLPLEFDDAFVEKVRRSLPELPDDKKNRFIADFG
LTPYDASVLVVDRENAAFFEKVAEGRDGKTAANWVINNLFGALAKAGKGVADSPVSAENLGKLLDLLKDEVINGRIAKDV
FEIMMETGEDPDAIVEDKGLRQVTDTSAIEAAVDAVIAANPEKADDVRGGKEKLLGWFVGQVMKATQGKANPAMLNDILR
AKLLG
>Mature_484_residues
TYLIKGETATWEVVVGLEVHAQVVSQSKLFSGSSTRFGAPANSQVSLVDAAMPGMLPVINGLCVEQAVRTGLGLRAKINK
RSIFARKNYFYADLPQGYQISQYEQPIVGEGKVVLDMPDGSSREVGIERLHLEQDAGKSMHDQHPTKSLIDLNRSGVALM
EIVSKPDMREPEEAGAYLRKLRSIMRYLGTCDGNMEEGSMRADVNVSVRPIGTTDLRTRCEIKNVNSVRFVMQAIEAEAH
RQVEVWESGGEVRQETRLFDPAKGETRSMRSKEHAHDYRYFPDPDLLPLEFDDAFVEKVRRSLPELPDDKKNRFIADFGL
TPYDASVLVVDRENAAFFEKVAEGRDGKTAANWVINNLFGALAKAGKGVADSPVSAENLGKLLDLLKDEVINGRIAKDVF
EIMMETGEDPDAIVEDKGLRQVTDTSAIEAAVDAVIAANPEKADDVRGGKEKLLGWFVGQVMKATQGKANPAMLNDILRA
KLLG

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily

Homologues:

Organism=Homo sapiens, GI4758894, Length=500, Percent_Identity=42.6, Blast_Score=372, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI115534094, Length=300, Percent_Identity=41, Blast_Score=206, Evalue=3e-53,
Organism=Saccharomyces cerevisiae, GI6319391, Length=513, Percent_Identity=32.1637426900585, Blast_Score=226, Evalue=5e-60,
Organism=Drosophila melanogaster, GI45550808, Length=481, Percent_Identity=37.8378378378378, Blast_Score=319, Evalue=3e-87,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATB_RHORT (Q2RPH5)

Other databases:

- EMBL:   CP000230
- RefSeq:   YP_428257.1
- ProteinModelPortal:   Q2RPH5
- SMR:   Q2RPH5
- STRING:   Q2RPH5
- GeneID:   3836621
- GenomeReviews:   CP000230_GR
- KEGG:   rru:Rru_A3175
- NMPDR:   fig|1085.1.peg.817
- eggNOG:   COG0064
- HOGENOM:   HBG395951
- OMA:   LRYLGTC
- PhylomeDB:   Q2RPH5
- ProtClustDB:   PRK05477
- BioCyc:   RRUB269796:RRU_A3175-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00121
- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958
- PANTHER:   PTHR11659
- SMART:   SM00845
- TIGRFAMs:   TIGR00133

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey; SSF89095 GatB_Yqey

EC number: 6.3.5.-

Molecular weight: Translated: 53439; Mature: 53308

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTYLIKGETATWEVVVGLEVHAQVVSQSKLFSGSSTRFGAPANSQVSLVDAAMPGMLPVI
CEEEEECCCCEEEEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEHHHCCCHHHHH
NGLCVEQAVRTGLGLRAKINKRSIFARKNYFYADLPQGYQISQYEQPIVGEGKVVLDMPD
HHHHHHHHHHHCCCCEEEECCHHEEEECCCEEEECCCCCCCCCCCCCCCCCCEEEEECCC
GSSREVGIERLHLEQDAGKSMHDQHPTKSLIDLNRSGVALMEIVSKPDMREPEEAGAYLR
CCCCCCCHHHEEEHHHCCCCCCCCCCCHHHHHCCCCCHHHHHHHCCCCCCCHHHHHHHHH
KLRSIMRYLGTCDGNMEEGSMRADVNVSVRPIGTTDLRTRCEIKNVNSVRFVMQAIEAEA
HHHHHHHHHHCCCCCCCCCCEEECCEEEEEECCCCCCCCEEECCCCHHHHHHHHHHHHHH
HRQVEVWESGGEVRQETRLFDPAKGETRSMRSKEHAHDYRYFPDPDLLPLEFDDAFVEKV
HCEEEEECCCCHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEECCCHHHHHHH
RRSLPELPDDKKNRFIADFGLTPYDASVLVVDRENAAFFEKVAEGRDGKTAANWVINNLF
HHHCCCCCCCCCCCEEEECCCCCCCCEEEEEECCCHHHHHHHHCCCCCCHHHHHHHHHHH
GALAKAGKGVADSPVSAENLGKLLDLLKDEVINGRIAKDVFEIMMETGEDPDAIVEDKGL
HHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCHHHCCCCC
RQVTDTSAIEAAVDAVIAANPEKADDVRGGKEKLLGWFVGQVMKATQGKANPAMLNDILR
HHHHHHHHHHHHHHHHHCCCCCHHHHCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
AKLLG
HHHCC
>Mature Secondary Structure 
TYLIKGETATWEVVVGLEVHAQVVSQSKLFSGSSTRFGAPANSQVSLVDAAMPGMLPVI
EEEEECCCCEEEEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEHHHCCCHHHHH
NGLCVEQAVRTGLGLRAKINKRSIFARKNYFYADLPQGYQISQYEQPIVGEGKVVLDMPD
HHHHHHHHHHHCCCCEEEECCHHEEEECCCEEEECCCCCCCCCCCCCCCCCCEEEEECCC
GSSREVGIERLHLEQDAGKSMHDQHPTKSLIDLNRSGVALMEIVSKPDMREPEEAGAYLR
CCCCCCCHHHEEEHHHCCCCCCCCCCCHHHHHCCCCCHHHHHHHCCCCCCCHHHHHHHHH
KLRSIMRYLGTCDGNMEEGSMRADVNVSVRPIGTTDLRTRCEIKNVNSVRFVMQAIEAEA
HHHHHHHHHHCCCCCCCCCCEEECCEEEEEECCCCCCCCEEECCCCHHHHHHHHHHHHHH
HRQVEVWESGGEVRQETRLFDPAKGETRSMRSKEHAHDYRYFPDPDLLPLEFDDAFVEKV
HCEEEEECCCCHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEECCCHHHHHHH
RRSLPELPDDKKNRFIADFGLTPYDASVLVVDRENAAFFEKVAEGRDGKTAANWVINNLF
HHHCCCCCCCCCCCEEEECCCCCCCCEEEEEECCCHHHHHHHHCCCCCCHHHHHHHHHHH
GALAKAGKGVADSPVSAENLGKLLDLLKDEVINGRIAKDVFEIMMETGEDPDAIVEDKGL
HHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCHHHCCCCC
RQVTDTSAIEAAVDAVIAANPEKADDVRGGKEKLLGWFVGQVMKATQGKANPAMLNDILR
HHHHHHHHHHHHHHHHHCCCCCHHHHCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
AKLLG
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA