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The map label for this gene is gatA

Identifier: 83594506

GI number: 83594506

Start: 3668276

End: 3669778

Strand: Reverse

Name: gatA

Synonym: Rru_A3176

Alternate gene names: 83594506

Gene position: 3669778-3668276 (Counterclockwise)

Preceding gene: 83594507

Following gene: 83594505

Centisome position: 84.31

GC content: 67.4

Gene sequence:

>1503_bases
ATGACCAATGCGACCACCCTGACCGATCTGACCATCGCCGAGGCCCGCGATGCCATGGCCAAGGGCGCGTTCACCCCGCT
TGAGCTGACCAACGCCCATTTGGCGCGGATGGAGGCCGGACGGGGGCTCAACGCCTTCATCACCGAAACCCCCGATCTGG
CCCGCATGCAGGCCGAACGCAGCGGCGAGCGCCTGCGCGCCGGCAAGGCCGGGCCGATGGAAGGCATTCCCATCGGCATC
AAGGATCTGTTCTGCACCGAAGGCGTGCGCACCACCGCCGGATCGAAGATCCTCGATGGCTTCGTCCCGCCTTACGAAAG
CACGGTCAGCGCCAATCTGGCCAAGGCCGGCGCGGTGATGCTGGGCAAGACCAATCTCGATGAATTCGCCATGGGCTCGT
CGAACAAGACCAGCGCCAGCGGCCCGGTGATCAATCCGTGGACGCGCACCGATGACGACGAGCAACTGGTTCCCGGCGGG
TCGTCGGGCGGTTCGGCCGCCGCCGTCGCCGGGCGCTTGGCGATGGCCGCCACCGGCACCGATACGGGCGGGTCAATCCG
CCAGCCCGCCGCCTTCTGCGGCCTTGTCGGCCTGAAGCCGACCTATGGGCGCTGCTCGCGCTGGGGCGTGGTGGCCTTCG
CCAGTTCGCTCGATCAGGCCGGGCCGATGACCCGCACGGTCCGCGACGCGGCGATCATGCTCAAGGCCATGGCCGGCCAT
GATCCGATGGACGGCACCAGCGCCCCGGTCGAGGTGCCGAATTTCGAGGCGGTGCTAAGCGGCGATATCCGCGGCTTGCG
CGTTGGCCTGCCCCGCCAGTACCGGCCCGAAGGCCTTGATCCCGAGATCGCCGCCCTGTGGGATCGCGGCGCCGAGATGC
TGAAGGACGCCGGCGCCACGCTGGTCGAGGTCGATCTGCCCCATACCAAATACGCCCTGGCGACCTATTACATCGTCGCC
CCGGCCGAAGCCTCGTCGAACCTCGCGCGCTATGACGGCGTGCGCTATGGCCTGCGGGTGCCGGGCGAGACGCTTGACGA
GATGTATTGCAAGACGCGGGCGGCCGGCTTTGGCGAAGAGGTGCGCCGCCGCGTGCTGATCGGCACCTATGCCCTGTCGG
CGGGCTATTACGACGCCTATTACCTGAAGGCGCAGAAGGTCCGCGCCCTGATCGCCCAGGACTTCAAGACCGCCTTCGAG
ACGGTCGACGTGCTGTTGACGCCGACGACGCCGAGCGCCGCCTTCGGCCTGAACGAAACGATCGACGATCCGATCACCAT
GTATCTCAACGACGTGTTCACCGTGCCGACCTCGCTGGCCGGTCTGCCGGGACTGTCGGTGCCCGTCGGCCTGTCGTCGC
GCGGCCTGCCTTTGGGCCTGCAGCTGGTTGGACGCTATTTCGACGAGGAAACCGTGCTCAACGCCGGTCTGGCCCTGGAA
CGGGCCAGCGCCTTCACCCAGAAGCCAGCGTTCCTGACCGGCGCTTTGGAAGGGCAGATCTGA

Upstream 100 bases:

>100_bases
CCGTTACCGACGGCGGCTATGCCGATCGCGTGCTGGCCAATGCTCCCGAAAGCCAGGACGGCTGTTTCCTGGTGCCCAAG
GTGGTGGAGTGAGCCCCGTC

Downstream 100 bases:

>100_bases
TGACCTATCTCATCAAAGGGGAAACCGCGACCTGGGAAGTGGTTGTCGGTCTGGAAGTCCATGCCCAGGTCGTCAGCCAG
TCCAAGCTGTTCTCGGGCTC

Product: aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A

Number of amino acids: Translated: 500; Mature: 499

Protein sequence:

>500_residues
MTNATTLTDLTIAEARDAMAKGAFTPLELTNAHLARMEAGRGLNAFITETPDLARMQAERSGERLRAGKAGPMEGIPIGI
KDLFCTEGVRTTAGSKILDGFVPPYESTVSANLAKAGAVMLGKTNLDEFAMGSSNKTSASGPVINPWTRTDDDEQLVPGG
SSGGSAAAVAGRLAMAATGTDTGGSIRQPAAFCGLVGLKPTYGRCSRWGVVAFASSLDQAGPMTRTVRDAAIMLKAMAGH
DPMDGTSAPVEVPNFEAVLSGDIRGLRVGLPRQYRPEGLDPEIAALWDRGAEMLKDAGATLVEVDLPHTKYALATYYIVA
PAEASSNLARYDGVRYGLRVPGETLDEMYCKTRAAGFGEEVRRRVLIGTYALSAGYYDAYYLKAQKVRALIAQDFKTAFE
TVDVLLTPTTPSAAFGLNETIDDPITMYLNDVFTVPTSLAGLPGLSVPVGLSSRGLPLGLQLVGRYFDEETVLNAGLALE
RASAFTQKPAFLTGALEGQI

Sequences:

>Translated_500_residues
MTNATTLTDLTIAEARDAMAKGAFTPLELTNAHLARMEAGRGLNAFITETPDLARMQAERSGERLRAGKAGPMEGIPIGI
KDLFCTEGVRTTAGSKILDGFVPPYESTVSANLAKAGAVMLGKTNLDEFAMGSSNKTSASGPVINPWTRTDDDEQLVPGG
SSGGSAAAVAGRLAMAATGTDTGGSIRQPAAFCGLVGLKPTYGRCSRWGVVAFASSLDQAGPMTRTVRDAAIMLKAMAGH
DPMDGTSAPVEVPNFEAVLSGDIRGLRVGLPRQYRPEGLDPEIAALWDRGAEMLKDAGATLVEVDLPHTKYALATYYIVA
PAEASSNLARYDGVRYGLRVPGETLDEMYCKTRAAGFGEEVRRRVLIGTYALSAGYYDAYYLKAQKVRALIAQDFKTAFE
TVDVLLTPTTPSAAFGLNETIDDPITMYLNDVFTVPTSLAGLPGLSVPVGLSSRGLPLGLQLVGRYFDEETVLNAGLALE
RASAFTQKPAFLTGALEGQI
>Mature_499_residues
TNATTLTDLTIAEARDAMAKGAFTPLELTNAHLARMEAGRGLNAFITETPDLARMQAERSGERLRAGKAGPMEGIPIGIK
DLFCTEGVRTTAGSKILDGFVPPYESTVSANLAKAGAVMLGKTNLDEFAMGSSNKTSASGPVINPWTRTDDDEQLVPGGS
SGGSAAAVAGRLAMAATGTDTGGSIRQPAAFCGLVGLKPTYGRCSRWGVVAFASSLDQAGPMTRTVRDAAIMLKAMAGHD
PMDGTSAPVEVPNFEAVLSGDIRGLRVGLPRQYRPEGLDPEIAALWDRGAEMLKDAGATLVEVDLPHTKYALATYYIVAP
AEASSNLARYDGVRYGLRVPGETLDEMYCKTRAAGFGEEVRRRVLIGTYALSAGYYDAYYLKAQKVRALIAQDFKTAFET
VDVLLTPTTPSAAFGLNETIDDPITMYLNDVFTVPTSLAGLPGLSVPVGLSSRGLPLGLQLVGRYFDEETVLNAGLALER
ASAFTQKPAFLTGALEGQI

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family

Homologues:

Organism=Homo sapiens, GI222831590, Length=497, Percent_Identity=43.2595573440644, Blast_Score=382, Evalue=1e-106,
Organism=Homo sapiens, GI195972892, Length=186, Percent_Identity=33.8709677419355, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI166795287, Length=287, Percent_Identity=29.2682926829268, Blast_Score=71, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17543272, Length=444, Percent_Identity=38.963963963964, Blast_Score=292, Evalue=3e-79,
Organism=Caenorhabditis elegans, GI17537465, Length=502, Percent_Identity=26.0956175298805, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17556264, Length=237, Percent_Identity=30.8016877637131, Blast_Score=86, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI71990152, Length=333, Percent_Identity=26.1261261261261, Blast_Score=83, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17538252, Length=273, Percent_Identity=27.1062271062271, Blast_Score=80, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6323950, Length=431, Percent_Identity=37.3549883990719, Blast_Score=254, Evalue=3e-68,
Organism=Saccharomyces cerevisiae, GI6319685, Length=435, Percent_Identity=28.735632183908, Blast_Score=117, Evalue=5e-27,
Organism=Drosophila melanogaster, GI24648113, Length=488, Percent_Identity=39.1393442622951, Blast_Score=331, Evalue=8e-91,
Organism=Drosophila melanogaster, GI24652985, Length=513, Percent_Identity=25.7309941520468, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI19922090, Length=513, Percent_Identity=25.7309941520468, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24652981, Length=513, Percent_Identity=25.7309941520468, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24652983, Length=513, Percent_Identity=25.7309941520468, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24644968, Length=499, Percent_Identity=24.0480961923848, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI161078093, Length=514, Percent_Identity=26.6536964980545, Blast_Score=103, Evalue=4e-22,
Organism=Drosophila melanogaster, GI21356731, Length=491, Percent_Identity=25.2545824847251, Blast_Score=83, Evalue=4e-16,
Organism=Drosophila melanogaster, GI45550774, Length=182, Percent_Identity=29.1208791208791, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24648435, Length=182, Percent_Identity=29.1208791208791, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24648437, Length=182, Percent_Identity=29.1208791208791, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24648441, Length=182, Percent_Identity=29.1208791208791, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24648439, Length=182, Percent_Identity=29.1208791208791, Blast_Score=74, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATA_RHORT (Q2RPH4)

Other databases:

- EMBL:   CP000230
- RefSeq:   YP_428258.1
- ProteinModelPortal:   Q2RPH4
- SMR:   Q2RPH4
- STRING:   Q2RPH4
- GeneID:   3836622
- GenomeReviews:   CP000230_GR
- KEGG:   rru:Rru_A3176
- NMPDR:   fig|1085.1.peg.818
- eggNOG:   COG0154
- HOGENOM:   HBG481888
- OMA:   RWGLIAF
- PhylomeDB:   Q2RPH4
- BioCyc:   RRUB269796:RRU_A3176-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00120
- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412
- Gene3D:   G3DSA:3.90.1300.10
- PANTHER:   PTHR11895
- TIGRFAMs:   TIGR00132

Pfam domain/function: PF01425 Amidase; SSF75304 Amidase_sig_enz

EC number: 6.3.5.-

Molecular weight: Translated: 52781; Mature: 52649

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: PS00571 AMIDASES

Important sites: ACT_SITE 81-81 ACT_SITE 161-161 ACT_SITE 185-185

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNATTLTDLTIAEARDAMAKGAFTPLELTNAHLARMEAGRGLNAFITETPDLARMQAER
CCCCCEEEEEHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHH
SGERLRAGKAGPMEGIPIGIKDLFCTEGVRTTAGSKILDGFVPPYESTVSANLAKAGAVM
CCHHHHCCCCCCCCCCCCCHHHHHHCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHCCEEE
LGKTNLDEFAMGSSNKTSASGPVINPWTRTDDDEQLVPGGSSGGSAAAVAGRLAMAATGT
EECCCCHHHHCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCHHHHHHHEEEEECCC
DTGGSIRQPAAFCGLVGLKPTYGRCSRWGVVAFASSLDQAGPMTRTVRDAAIMLKAMAGH
CCCCCCCCCHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCC
DPMDGTSAPVEVPNFEAVLSGDIRGLRVGLPRQYRPEGLDPEIAALWDRGAEMLKDAGAT
CCCCCCCCCCCCCCCCEEECCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCE
LVEVDLPHTKYALATYYIVAPAEASSNLARYDGVRYGLRVPGETLDEMYCKTRAAGFGEE
EEEEECCCCCEEEEEEEEEECCCCCCCHHHHCCEEEEEECCCHHHHHHHHHHHCCCCCHH
VRRRVLIGTYALSAGYYDAYYLKAQKVRALIAQDFKTAFETVDVLLTPTTPSAAFGLNET
HHHHHEEEEEECCCCCCCCEEHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCHHCCCCCC
IDDPITMYLNDVFTVPTSLAGLPGLSVPVGLSSRGLPLGLQLVGRYFDEETVLNAGLALE
CCCCHHHHHHHHHHCCHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHCHHHHHHHCHHHH
RASAFTQKPAFLTGALEGQI
HHHHHHCCCCEEEECCCCCC
>Mature Secondary Structure 
TNATTLTDLTIAEARDAMAKGAFTPLELTNAHLARMEAGRGLNAFITETPDLARMQAER
CCCCEEEEEHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHH
SGERLRAGKAGPMEGIPIGIKDLFCTEGVRTTAGSKILDGFVPPYESTVSANLAKAGAVM
CCHHHHCCCCCCCCCCCCCHHHHHHCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHCCEEE
LGKTNLDEFAMGSSNKTSASGPVINPWTRTDDDEQLVPGGSSGGSAAAVAGRLAMAATGT
EECCCCHHHHCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCHHHHHHHEEEEECCC
DTGGSIRQPAAFCGLVGLKPTYGRCSRWGVVAFASSLDQAGPMTRTVRDAAIMLKAMAGH
CCCCCCCCCHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCC
DPMDGTSAPVEVPNFEAVLSGDIRGLRVGLPRQYRPEGLDPEIAALWDRGAEMLKDAGAT
CCCCCCCCCCCCCCCCEEECCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCE
LVEVDLPHTKYALATYYIVAPAEASSNLARYDGVRYGLRVPGETLDEMYCKTRAAGFGEE
EEEEECCCCCEEEEEEEEEECCCCCCCHHHHCCEEEEEECCCHHHHHHHHHHHCCCCCHH
VRRRVLIGTYALSAGYYDAYYLKAQKVRALIAQDFKTAFETVDVLLTPTTPSAAFGLNET
HHHHHEEEEEECCCCCCCCEEHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCHHCCCCCC
IDDPITMYLNDVFTVPTSLAGLPGLSVPVGLSSRGLPLGLQLVGRYFDEETVLNAGLALE
CCCCHHHHHHHHHHCCHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHCHHHHHHHCHHHH
RASAFTQKPAFLTGALEGQI
HHHHHHCCCCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA