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The map label for this gene is asnB [H]

Identifier: 83594451

GI number: 83594451

Start: 3600181

End: 3602106

Strand: Direct

Name: asnB [H]

Synonym: Rru_A3121

Alternate gene names: 83594451

Gene position: 3600181-3602106 (Clockwise)

Preceding gene: 83594450

Following gene: 83594452

Centisome position: 82.71

GC content: 67.19

Gene sequence:

>1926_bases
ATGTGTGGACTCGTCGGCATCTACCATCCCACCGCCTCCCGGCCGGTCGATCGCGCCCTGGTCGAGGCGATGAACCAGCG
CCAGTTTCACCGGGGTCCCGATGACGGCGGCGTCTTCATCGGGCCGGGCATCGGCCTTGGCCATCGCCGCCTGTCGATCG
TCGACCTGGGCGGTGGCCGCCAGCCGATGACCAACGACGGCGGCGGCGTGGTGCTCGTCTATAATGGCGAGCTTTACAAC
AATGCCGAGCTGGTGGCCGAGCTGAAGGCCGCCGGCCATGTCTTCCGCACCGCCAGCGACACCGAGACCGTGATCCACGC
CTGGGAGGAATGGGGCGAGGACTCGGTGCGCCATCTGCGCGGCATGTTCGCCTATGCGCTGTGGGACGACCGCAGCCAGA
CCTTGTTCCTGGCCCGCGACCGCTTAGGCATCAAGCCGCTGCATTACACCTGTTTGGCCGATGGCCGGGTGATGTTCGGC
TCCGAGCTGAAAAGCCTGACCGGTGTCGAAGATATCCCCAAGGTCGTCGATCCCCTGGCGATCGAGGATTTCCTGGCCTA
TGGCTATGTTCCCGACCCGCGCTCGATCTTCAAGGATATCCTCAAGCTTGAAGCCGGCCATACCCTGACCTTCCGCCGGG
GCGAGGCGCCGGGCCGGCCCCACTGCTATTGGGATGTCAGCTTCACCACCCCGAGCCGCCCGGCCAAGACCGACGACTTG
TGCGCCGAGGTGATCGACCGCCTGGACGAGGCGGTGCGCTGTCATCTGGTCGCCGATGTTCCCGTCGGCGCCTTCCTGTC
GGGGGGCGTTGATTCCAGCGCCATGGTCGCCCTGATGGCCGGGGCCACCGACCGCCCGGTGGAAACCTGCTCGATCGCCT
TTTCCGAGGCCCGCTTCGACGAATCGGCCTATGCCAAAACCGTCGCCGAGCGCTATGGCACCCACCACCGGGTCCATACC
CTGCCCGCCCAGGCGCTGAGCGAGGTCGGGCCGCTGACGGCGATCTACGACGAACCCTTCGCCGACAGTTCGGCCCTGCC
GACCCTCAAGGTCTGTCAGGCGGCGCGCGAAACCGTGACCGTCGCCCTGTCGGGCGATGGCGGGGACGAGCTTTTCGCCG
GCTATCGCCGCTACCGCTTCCATACCGCCGAAGAGGCGGTGCGCCGCATCCTGCCCCAGGCCGCCCGTGGGCCGCTGTTT
GGCGCCCTGGGCCGCGTCTATCCCAAGCTTGATTGGGCCCCCCAGGTTCTGCGCGCCAAATCCACCTTCCAGGCCCTGGG
CCGCGACCGGGTGGAGGGCTATTTCAACGCCGTTTCGGTGATCCCCGACGGCCTGCGCCGCGGCTTGTACAGCAAGACCA
TGCGGGCCGAGCTTGCCGGCTATGGCGCCTCGGAGGTTCTGCGCCGCCATATGACGCGGGCGCCGGCGGATCATCCGCTG
TCCATCGCCCAATATGTCGATCTCAAAACTTGGTTGCCCGGCGACATCCTGACCAAGGTTGATCGCGCCAGCATGGCCAC
CAGTCTGGAGGTCCGGGTGCCGATGCTCGATCACCGCTTCGTCGAATGGACCGCCGGCCTGTCCCCCGACCTCAAGCTGT
CGGGGGGAACGGGCAAATACCTGCTCAAACGGGCGCTGCGGCCCCTGCTGCCCGATGAGGTGCTTTATCGCCGCAAGATG
GGCTTCTCCATTCCGCTGACCGACTGGCTGCGCGGCCCGTGGTCGGGGCGGACCCGCGCCCTGGCCGGCCGATCGGCCCT
GGTGACCAGCGGCCTGTTCGATGGCGCCGCCGTCGCAAAGCTGGTCGAGCAGCACCTGGGGGGGAACTGGGATCATGGCG
CGGCGCTTTATGCGCTGATCGTGCTCGATGATTTCCTGACCGCCCATGTCGGCGCGGCCTGCCTGCCGGCAAGGATGAGC
GCATGA

Upstream 100 bases:

>100_bases
AGGCTCTCGACGACGCCCGGGCCCTGCTTGGCCGCTTCCTCGCCGGCCAAGGGGATCTCGGCCCGGGCCTCGCCCTCGCC
CAAGAGAAGGACCGCTGAGC

Downstream 100 bases:

>100_bases
CCACCCCCGGCGCCCCCCTGGGAACCGCCCCGTCGCCAACCGCCGGCATCGTCATCGCCCTGTTGCGCGAGACCTTGCAG
ATCGGCGAGCGGGCCGACGG

Product: asparagine synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 641; Mature: 641

Protein sequence:

>641_residues
MCGLVGIYHPTASRPVDRALVEAMNQRQFHRGPDDGGVFIGPGIGLGHRRLSIVDLGGGRQPMTNDGGGVVLVYNGELYN
NAELVAELKAAGHVFRTASDTETVIHAWEEWGEDSVRHLRGMFAYALWDDRSQTLFLARDRLGIKPLHYTCLADGRVMFG
SELKSLTGVEDIPKVVDPLAIEDFLAYGYVPDPRSIFKDILKLEAGHTLTFRRGEAPGRPHCYWDVSFTTPSRPAKTDDL
CAEVIDRLDEAVRCHLVADVPVGAFLSGGVDSSAMVALMAGATDRPVETCSIAFSEARFDESAYAKTVAERYGTHHRVHT
LPAQALSEVGPLTAIYDEPFADSSALPTLKVCQAARETVTVALSGDGGDELFAGYRRYRFHTAEEAVRRILPQAARGPLF
GALGRVYPKLDWAPQVLRAKSTFQALGRDRVEGYFNAVSVIPDGLRRGLYSKTMRAELAGYGASEVLRRHMTRAPADHPL
SIAQYVDLKTWLPGDILTKVDRASMATSLEVRVPMLDHRFVEWTAGLSPDLKLSGGTGKYLLKRALRPLLPDEVLYRRKM
GFSIPLTDWLRGPWSGRTRALAGRSALVTSGLFDGAAVAKLVEQHLGGNWDHGAALYALIVLDDFLTAHVGAACLPARMS
A

Sequences:

>Translated_641_residues
MCGLVGIYHPTASRPVDRALVEAMNQRQFHRGPDDGGVFIGPGIGLGHRRLSIVDLGGGRQPMTNDGGGVVLVYNGELYN
NAELVAELKAAGHVFRTASDTETVIHAWEEWGEDSVRHLRGMFAYALWDDRSQTLFLARDRLGIKPLHYTCLADGRVMFG
SELKSLTGVEDIPKVVDPLAIEDFLAYGYVPDPRSIFKDILKLEAGHTLTFRRGEAPGRPHCYWDVSFTTPSRPAKTDDL
CAEVIDRLDEAVRCHLVADVPVGAFLSGGVDSSAMVALMAGATDRPVETCSIAFSEARFDESAYAKTVAERYGTHHRVHT
LPAQALSEVGPLTAIYDEPFADSSALPTLKVCQAARETVTVALSGDGGDELFAGYRRYRFHTAEEAVRRILPQAARGPLF
GALGRVYPKLDWAPQVLRAKSTFQALGRDRVEGYFNAVSVIPDGLRRGLYSKTMRAELAGYGASEVLRRHMTRAPADHPL
SIAQYVDLKTWLPGDILTKVDRASMATSLEVRVPMLDHRFVEWTAGLSPDLKLSGGTGKYLLKRALRPLLPDEVLYRRKM
GFSIPLTDWLRGPWSGRTRALAGRSALVTSGLFDGAAVAKLVEQHLGGNWDHGAALYALIVLDDFLTAHVGAACLPARMS
A
>Mature_641_residues
MCGLVGIYHPTASRPVDRALVEAMNQRQFHRGPDDGGVFIGPGIGLGHRRLSIVDLGGGRQPMTNDGGGVVLVYNGELYN
NAELVAELKAAGHVFRTASDTETVIHAWEEWGEDSVRHLRGMFAYALWDDRSQTLFLARDRLGIKPLHYTCLADGRVMFG
SELKSLTGVEDIPKVVDPLAIEDFLAYGYVPDPRSIFKDILKLEAGHTLTFRRGEAPGRPHCYWDVSFTTPSRPAKTDDL
CAEVIDRLDEAVRCHLVADVPVGAFLSGGVDSSAMVALMAGATDRPVETCSIAFSEARFDESAYAKTVAERYGTHHRVHT
LPAQALSEVGPLTAIYDEPFADSSALPTLKVCQAARETVTVALSGDGGDELFAGYRRYRFHTAEEAVRRILPQAARGPLF
GALGRVYPKLDWAPQVLRAKSTFQALGRDRVEGYFNAVSVIPDGLRRGLYSKTMRAELAGYGASEVLRRHMTRAPADHPL
SIAQYVDLKTWLPGDILTKVDRASMATSLEVRVPMLDHRFVEWTAGLSPDLKLSGGTGKYLLKRALRPLLPDEVLYRRKM
GFSIPLTDWLRGPWSGRTRALAGRSALVTSGLFDGAAVAKLVEQHLGGNWDHGAALYALIVLDDFLTAHVGAACLPARMS
A

Specific function: Main asparagine synthetase in vegetative cells [H]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1786889, Length=413, Percent_Identity=29.7820823244552, Blast_Score=147, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI71993933, Length=412, Percent_Identity=29.6116504854369, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI25147557, Length=412, Percent_Identity=29.8543689320388, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI25147560, Length=354, Percent_Identity=29.9435028248588, Blast_Score=103, Evalue=4e-22,
Organism=Saccharomyces cerevisiae, GI6325403, Length=434, Percent_Identity=29.9539170506912, Blast_Score=124, Evalue=5e-29,
Organism=Saccharomyces cerevisiae, GI6321563, Length=436, Percent_Identity=27.9816513761468, Blast_Score=119, Evalue=2e-27,
Organism=Drosophila melanogaster, GI45553209, Length=421, Percent_Identity=29.4536817102138, Blast_Score=141, Evalue=1e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 70061; Mature: 70061

Theoretical pI: Translated: 7.19; Mature: 7.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGLVGIYHPTASRPVDRALVEAMNQRQFHRGPDDGGVFIGPGIGLGHRRLSIVDLGGGR
CCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCEEEEEECCCCC
QPMTNDGGGVVLVYNGELYNNAELVAELKAAGHVFRTASDTETVIHAWEEWGEDSVRHLR
CCCCCCCCCEEEEECCCCCCCHHHHHHHHHHCHHEECCCCHHHHHHHHHHHCHHHHHHHH
GMFAYALWDDRSQTLFLARDRLGIKPLHYTCLADGRVMFGSELKSLTGVEDIPKVVDPLA
HHHHHEEECCCCCEEEEEECCCCCCCEEEEEEECCEEEECHHHHHHCCHHHHHHHHHHHH
IEDFLAYGYVPDPRSIFKDILKLEAGHTLTFRRGEAPGRPHCYWDVSFTTPSRPAKTDDL
HHHHHHHCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEEEECCCCCCCCCHHH
CAEVIDRLDEAVRCHLVADVPVGAFLSGGVDSSAMVALMAGATDRPVETCSIAFSEARFD
HHHHHHHHHHHHEEEEEECCCHHHHHCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHCC
ESAYAKTVAERYGTHHRVHTLPAQALSEVGPLTAIYDEPFADSSALPTLKVCQAARETVT
HHHHHHHHHHHCCCCCEEEECHHHHHHHHCCEEEECCCCCCCCCCCCHHHHHHHHHHEEE
VALSGDGGDELFAGYRRYRFHTAEEAVRRILPQAARGPLFGALGRVYPKLDWAPQVLRAK
EEEECCCCHHHHHHHHHHHEECHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCHHHHHHH
STFQALGRDRVEGYFNAVSVIPDGLRRGLYSKTMRAELAGYGASEVLRRHMTRAPADHPL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCC
SIAQYVDLKTWLPGDILTKVDRASMATSLEVRVPMLDHRFVEWTAGLSPDLKLSGGTGKY
HHHHHHHHHHCCCHHHHHHHHHHHHHCEEEEECCHHHCHHHHHHCCCCCCCEECCCCHHH
LLKRALRPLLPDEVLYRRKMGFSIPLTDWLRGPWSGRTRALAGRSALVTSGLFDGAAVAK
HHHHHHHCCCCHHHHHHHHCCCCCCHHHHHCCCCCCCCHHHCCCHHHHHHCCCCHHHHHH
LVEQHLGGNWDHGAALYALIVLDDFLTAHVGAACLPARMSA
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHCCC
>Mature Secondary Structure
MCGLVGIYHPTASRPVDRALVEAMNQRQFHRGPDDGGVFIGPGIGLGHRRLSIVDLGGGR
CCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCEEEEEECCCCC
QPMTNDGGGVVLVYNGELYNNAELVAELKAAGHVFRTASDTETVIHAWEEWGEDSVRHLR
CCCCCCCCCEEEEECCCCCCCHHHHHHHHHHCHHEECCCCHHHHHHHHHHHCHHHHHHHH
GMFAYALWDDRSQTLFLARDRLGIKPLHYTCLADGRVMFGSELKSLTGVEDIPKVVDPLA
HHHHHEEECCCCCEEEEEECCCCCCCEEEEEEECCEEEECHHHHHHCCHHHHHHHHHHHH
IEDFLAYGYVPDPRSIFKDILKLEAGHTLTFRRGEAPGRPHCYWDVSFTTPSRPAKTDDL
HHHHHHHCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEEEECCCCCCCCCHHH
CAEVIDRLDEAVRCHLVADVPVGAFLSGGVDSSAMVALMAGATDRPVETCSIAFSEARFD
HHHHHHHHHHHHEEEEEECCCHHHHHCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHCC
ESAYAKTVAERYGTHHRVHTLPAQALSEVGPLTAIYDEPFADSSALPTLKVCQAARETVT
HHHHHHHHHHHCCCCCEEEECHHHHHHHHCCEEEECCCCCCCCCCCCHHHHHHHHHHEEE
VALSGDGGDELFAGYRRYRFHTAEEAVRRILPQAARGPLFGALGRVYPKLDWAPQVLRAK
EEEECCCCHHHHHHHHHHHEECHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCHHHHHHH
STFQALGRDRVEGYFNAVSVIPDGLRRGLYSKTMRAELAGYGASEVLRRHMTRAPADHPL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCC
SIAQYVDLKTWLPGDILTKVDRASMATSLEVRVPMLDHRFVEWTAGLSPDLKLSGGTGKY
HHHHHHHHHHCCCHHHHHHHHHHHHHCEEEEECCHHHCHHHHHHCCCCCCCEECCCCHHH
LLKRALRPLLPDEVLYRRKMGFSIPLTDWLRGPWSGRTRALAGRSALVTSGLFDGAAVAK
HHHHHHHCCCCHHHHHHHHCCCCCCHHHHHCCCCCCCCHHHCCCHHHHHHCCCCHHHHHH
LVEQHLGGNWDHGAALYALIVLDDFLTAHVGAACLPARMSA
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377; 8755891; 10498721 [H]