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The map label for this gene is potB [H]

Identifier: 83594343

GI number: 83594343

Start: 3469973

End: 3470839

Strand: Reverse

Name: potB [H]

Synonym: Rru_A3013

Alternate gene names: 83594343

Gene position: 3470839-3469973 (Counterclockwise)

Preceding gene: 83594344

Following gene: 83594342

Centisome position: 79.74

GC content: 63.55

Gene sequence:

>867_bases
ATGGAGGCGCGGTCCAAACCCCCTGTGCTGACGGCTGGCGGGCGCTGCTCGTTGCCGGTTCTGACCCTGCTGCTGCTCGG
CTTCGTCGCCCCGCTGGTCGCCGTGATCGCCTTTTCCTTTGCCGAGCCCCGCAGCTTCGCCGCCTTTTCCAGCTTCACCC
TGGCCAATTACCGCGAGATCTTCGATCCGGCCAATACGGTGTGGATGTCGTTCGCCTGGTCGCTGGGGCTGGCGGGGGTG
ACGGTCGCCTTGCTTGGCGTTATCACCTATCCGATCGCCCTGGGTCTGGTGCGGGTTTTCGGGCGCTGGGCGCCGGTGAT
CAGCATTCTGTTCGTCTTCCCGCTGTTTATCTCGGAGAACGTCCGGCTCTATGGCTGGGTGCTGTTCTTCATCAAGAACG
GTGTGCTTGACGGCACCTTGCGGCTGCTGGGCGGTTCGGGGCCCGAGGTGCTGTATACCCCGGCGGCCACGCTGTTTGGC
ATGGTCTACACCTATCTGCCCTTCATGCTGTTTCCCACCGTGCTTGGCCTGTCGTTGCTGCCGCGCGACGTGGTCGACGC
CGCGCGCGATCTCGGGGCGAGCCGGCTGCAGGCGTGGTGGGAGGTGGAATTGCCCTTGGCGATGCCGGGCATTCTGATCG
GCACGCTGTTGACCTTCGTGCTGGCGGTCGGCGCCGTGGCCGAATCGAAGATCATGGGTGGGCAATCGTTGATCGTCATC
ACCCACGATATCGAAATCGCCTTCACCTATTCCCAGAACTGGCCGCTCGGCTCGGCTTTGGCGGTGCTGCTGACCCTGAT
CATCGGCGGCTTGACGCTGTTTGCCCTGTCCAAGCTCGATCTCGACCGCATTCTTGGGCGCAAGTGA

Upstream 100 bases:

>100_bases
TGGAACAGCCGCGTGGCGCGCTCTGCCCGCCGGGCGATGGCGGCGAGGTGCTGATCGGCTGGAACGCGGCCGATGCCGCC
TTCGTGGCGGAGTAAGGGCG

Downstream 100 bases:

>100_bases
GGATTCCGGCCATGGATATCAATCAAACCGACCGCCGCAATCGCGGGCTGCTCTGGGGCTGGACGCTGTTCGTGCTGGTG
GTCTTGTATCTGCCGGTGGT

Product: binding-protein dependent transport system inner membrane protein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 288; Mature: 288

Protein sequence:

>288_residues
MEARSKPPVLTAGGRCSLPVLTLLLLGFVAPLVAVIAFSFAEPRSFAAFSSFTLANYREIFDPANTVWMSFAWSLGLAGV
TVALLGVITYPIALGLVRVFGRWAPVISILFVFPLFISENVRLYGWVLFFIKNGVLDGTLRLLGGSGPEVLYTPAATLFG
MVYTYLPFMLFPTVLGLSLLPRDVVDAARDLGASRLQAWWEVELPLAMPGILIGTLLTFVLAVGAVAESKIMGGQSLIVI
THDIEIAFTYSQNWPLGSALAVLLTLIIGGLTLFALSKLDLDRILGRK

Sequences:

>Translated_288_residues
MEARSKPPVLTAGGRCSLPVLTLLLLGFVAPLVAVIAFSFAEPRSFAAFSSFTLANYREIFDPANTVWMSFAWSLGLAGV
TVALLGVITYPIALGLVRVFGRWAPVISILFVFPLFISENVRLYGWVLFFIKNGVLDGTLRLLGGSGPEVLYTPAATLFG
MVYTYLPFMLFPTVLGLSLLPRDVVDAARDLGASRLQAWWEVELPLAMPGILIGTLLTFVLAVGAVAESKIMGGQSLIVI
THDIEIAFTYSQNWPLGSALAVLLTLIIGGLTLFALSKLDLDRILGRK
>Mature_288_residues
MEARSKPPVLTAGGRCSLPVLTLLLLGFVAPLVAVIAFSFAEPRSFAAFSSFTLANYREIFDPANTVWMSFAWSLGLAGV
TVALLGVITYPIALGLVRVFGRWAPVISILFVFPLFISENVRLYGWVLFFIKNGVLDGTLRLLGGSGPEVLYTPAATLFG
MVYTYLPFMLFPTVLGLSLLPRDVVDAARDLGASRLQAWWEVELPLAMPGILIGTLLTFVLAVGAVAESKIMGGQSLIVI
THDIEIAFTYSQNWPLGSALAVLLTLIIGGLTLFALSKLDLDRILGRK

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787369, Length=262, Percent_Identity=32.824427480916, Blast_Score=125, Evalue=3e-30,
Organism=Escherichia coli, GI1787080, Length=220, Percent_Identity=29.5454545454545, Blast_Score=95, Evalue=5e-21,
Organism=Escherichia coli, GI1787713, Length=195, Percent_Identity=32.3076923076923, Blast_Score=75, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 31110; Mature: 31110

Theoretical pI: Translated: 8.42; Mature: 8.42

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEARSKPPVLTAGGRCSLPVLTLLLLGFVAPLVAVIAFSFAEPRSFAAFSSFTLANYREI
CCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
FDPANTVWMSFAWSLGLAGVTVALLGVITYPIALGLVRVFGRWAPVISILFVFPLFISEN
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
VRLYGWVLFFIKNGVLDGTLRLLGGSGPEVLYTPAATLFGMVYTYLPFMLFPTVLGLSLL
CHHHHHHHHHHHCCCHHHHHHEECCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHC
PRDVVDAARDLGASRLQAWWEVELPLAMPGILIGTLLTFVLAVGAVAESKIMGGQSLIVI
CHHHHHHHHHCCHHHHHHHHEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
THDIEIAFTYSQNWPLGSALAVLLTLIIGGLTLFALSKLDLDRILGRK
EEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MEARSKPPVLTAGGRCSLPVLTLLLLGFVAPLVAVIAFSFAEPRSFAAFSSFTLANYREI
CCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
FDPANTVWMSFAWSLGLAGVTVALLGVITYPIALGLVRVFGRWAPVISILFVFPLFISEN
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
VRLYGWVLFFIKNGVLDGTLRLLGGSGPEVLYTPAATLFGMVYTYLPFMLFPTVLGLSLL
CHHHHHHHHHHHCCCHHHHHHEECCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHC
PRDVVDAARDLGASRLQAWWEVELPLAMPGILIGTLLTFVLAVGAVAESKIMGGQSLIVI
CHHHHHHHHHCCHHHHHHHHEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
THDIEIAFTYSQNWPLGSALAVLLTLIIGGLTLFALSKLDLDRILGRK
EEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]