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The map label for this gene is act [H]

Identifier: 83594329

GI number: 83594329

Start: 3453398

End: 3454195

Strand: Reverse

Name: act [H]

Synonym: Rru_A2999

Alternate gene names: 83594329

Gene position: 3454195-3453398 (Counterclockwise)

Preceding gene: 83594330

Following gene: 83594328

Centisome position: 79.36

GC content: 66.92

Gene sequence:

>798_bases
ATGTTCGACCTCCCAGCGCCCCTCCCCCAAGTCCCCGCCGAAGCCGATCTGGTGTCTCCCCCCCTCCAGACACGCGGGTT
CCTCCATTCGGTGGAGACGGGGGCGGCCGTGGACGGCCCGGGCATGCGGTTTGTCTTCTTCCTGTCCGGCTGTCAGTTCC
GCTGCGCCTATTGCCACAACCCCGATACCTGGAAGCTTCATAACGGCCGTGCCCTGGATCTCGACGAGGCCATGGCCGAG
GTTTCCCCCTATGCCGGTTTCCTGCGCATCGCCGGCGGCGTCACCGTTTCGGGCGGCGAGCCGCTGATGCAGGCCGATTT
CACCGGGGCGCTGCTCGCCCGGCTCAAGGACCAGTTGGGCTTGCACACCGCGCTCGACACCCAGGGCTTTCTGCACGCCG
GGGTGAGCGACCGCTGGTTTGACCCGGTCGATCTGGTGCTGCTCGACATCAAGCATTCCGATCCCGAAGCCTATCACCGC
CTGACCGGTCAGGCCCTGGCTCCGACGCTGGCTTTCGCCCACCGTCTGGTCGATTTGGGCAAGCCGATGTGGATCCGCTA
TGTGCTGGTCCCCGGGCTGACCGACGGCGCCGACGATATCGATCGTCTGGCCGATTTCCTGGCTTGCCTTGGTCCGGCCG
TTCAGCGGGTCGAGGTCTTGCCGTTTCATCAGATGGGCGCGGCCAAATGGGCGCGCCTGGGGCTGACCTACAGCCTCGCC
CAAACCCCCACGCCAACCGCCCCCCAGGTCGACGCCGCCCGCGCCCGCTTCGCCGAGCGCGGCTTGAAGGTGTTTTGA

Upstream 100 bases:

>100_bases
TGATCCATCCCCTTTCCCGGGCCGCCGGTCGGCCCGGGATTTCGAACGGTGCGACGGGCCGGCAAGCCTCCCCGGTCCGT
CGCAGGAAGAGGACAAACCG

Downstream 100 bases:

>100_bases
AACACCCACCGCGGTCCGTGCCGCTAGCCCCTTTGGGACCACCGACATGATCCATGAGAACTCTCTGATCCTTGTGCTCA
ACTCCGGCAGTTCATCGCTG

Product: radical SAM family protein

Products: NA

Alternate protein names: PFL-activating enzyme; Formate-C-acetyltransferase-activating enzyme [H]

Number of amino acids: Translated: 265; Mature: 265

Protein sequence:

>265_residues
MFDLPAPLPQVPAEADLVSPPLQTRGFLHSVETGAAVDGPGMRFVFFLSGCQFRCAYCHNPDTWKLHNGRALDLDEAMAE
VSPYAGFLRIAGGVTVSGGEPLMQADFTGALLARLKDQLGLHTALDTQGFLHAGVSDRWFDPVDLVLLDIKHSDPEAYHR
LTGQALAPTLAFAHRLVDLGKPMWIRYVLVPGLTDGADDIDRLADFLACLGPAVQRVEVLPFHQMGAAKWARLGLTYSLA
QTPTPTAPQVDAARARFAERGLKVF

Sequences:

>Translated_265_residues
MFDLPAPLPQVPAEADLVSPPLQTRGFLHSVETGAAVDGPGMRFVFFLSGCQFRCAYCHNPDTWKLHNGRALDLDEAMAE
VSPYAGFLRIAGGVTVSGGEPLMQADFTGALLARLKDQLGLHTALDTQGFLHAGVSDRWFDPVDLVLLDIKHSDPEAYHR
LTGQALAPTLAFAHRLVDLGKPMWIRYVLVPGLTDGADDIDRLADFLACLGPAVQRVEVLPFHQMGAAKWARLGLTYSLA
QTPTPTAPQVDAARARFAERGLKVF
>Mature_265_residues
MFDLPAPLPQVPAEADLVSPPLQTRGFLHSVETGAAVDGPGMRFVFFLSGCQFRCAYCHNPDTWKLHNGRALDLDEAMAE
VSPYAGFLRIAGGVTVSGGEPLMQADFTGALLARLKDQLGLHTALDTQGFLHAGVSDRWFDPVDLVLLDIKHSDPEAYHR
LTGQALAPTLAFAHRLVDLGKPMWIRYVLVPGLTDGADDIDRLADFLACLGPAVQRVEVLPFHQMGAAKWARLGLTYSLA
QTPTPTAPQVDAARARFAERGLKVF

Specific function: Activation of pyruvate formate-lyase under anaerobic conditions by generation of an organic free radical, using S- adenosylmethionine and reduced flavodoxin as cosubstrates to produce 5'-deoxy-adenosine [H]

COG id: COG1180

COG function: function code O; Pyruvate-formate lyase-activating enzyme

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the organic radical-activating enzymes family [H]

Homologues:

Organism=Escherichia coli, GI1787130, Length=243, Percent_Identity=39.0946502057613, Blast_Score=188, Evalue=3e-49,
Organism=Escherichia coli, GI1790389, Length=268, Percent_Identity=29.8507462686567, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI1790839, Length=278, Percent_Identity=27.3381294964029, Blast_Score=84, Evalue=8e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR012838
- InterPro:   IPR001989
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =1.97.1.4 [H]

Molecular weight: Translated: 28769; Mature: 28769

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: PS01087 RADICAL_ACTIVATING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFDLPAPLPQVPAEADLVSPPLQTRGFLHSVETGAAVDGPGMRFVFFLSGCQFRCAYCHN
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEECCCCCEEEEEECCCCEEEEEECC
PDTWKLHNGRALDLDEAMAEVSPYAGFLRIAGGVTVSGGEPLMQADFTGALLARLKDQLG
CCCEEECCCCEECHHHHHHHHCHHHHHEEHCCCEEECCCCCCCHHHHHHHHHHHHHHHHC
LHTALDTQGFLHAGVSDRWFDPVDLVLLDIKHSDPEAYHRLTGQALAPTLAFAHRLVDLG
CCHHCCCCCCEECCCCCCCCCCEEEEEEEECCCCHHHHHHHCCHHHHHHHHHHHHHHHCC
KPMWIRYVLVPGLTDGADDIDRLADFLACLGPAVQRVEVLPFHQMGAAKWARLGLTYSLA
CCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHCEEEEEC
QTPTPTAPQVDAARARFAERGLKVF
CCCCCCCCCCHHHHHHHHHCCCCCC
>Mature Secondary Structure
MFDLPAPLPQVPAEADLVSPPLQTRGFLHSVETGAAVDGPGMRFVFFLSGCQFRCAYCHN
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEECCCCCEEEEEECCCCEEEEEECC
PDTWKLHNGRALDLDEAMAEVSPYAGFLRIAGGVTVSGGEPLMQADFTGALLARLKDQLG
CCCEEECCCCEECHHHHHHHHCHHHHHEEHCCCEEECCCCCCCHHHHHHHHHHHHHHHHC
LHTALDTQGFLHAGVSDRWFDPVDLVLLDIKHSDPEAYHRLTGQALAPTLAFAHRLVDLG
CCHHCCCCCCEECCCCCCCCCCEEEEEEEECCCCHHHHHHHCCHHHHHHHHHHHHHHHCC
KPMWIRYVLVPGLTDGADDIDRLADFLACLGPAVQRVEVLPFHQMGAAKWARLGLTYSLA
CCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHCEEEEEC
QTPTPTAPQVDAARARFAERGLKVF
CCCCCCCCCCHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11029425; 10899886; 12397186 [H]