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The map label for this gene is phrB [H]

Identifier: 83594235

GI number: 83594235

Start: 3345275

End: 3346711

Strand: Direct

Name: phrB [H]

Synonym: Rru_A2903

Alternate gene names: 83594235

Gene position: 3345275-3346711 (Clockwise)

Preceding gene: 83594234

Following gene: 83594238

Centisome position: 76.85

GC content: 69.1

Gene sequence:

>1437_bases
ATGACCATCCCCGTCCTCGTCTGGTTTCGCGACGACCTGCGGCTGGCCGATAATCCGGCCCTCTGCGCCGCCCTCGACAG
CGGGGCGCCGGTGATCCCGTTCTATCTGCTTGATGACAGCACCTCGGATGGCCACCGCCCGCTGGGCGGGGCGACCCGCT
GGTGGCTGGGGCGGTCCTTGACCTCGCTCGCCGAGTCCATCGCCCAGCGCGGCGGCCGCCTTGTGCTGCGTCGGGGCGAG
GCGTTGAGCGCGGTTCCCGAGCTCGCCGCCGAGACCGACGCCGCCGCCCTGTTCGCCAACCGTCTGTACGGCATGGACAG
CTTGCGCGATGACGATCTGTTCATGGTGCTGCGGGTGGCCGGGCTGACGGTCCGGCTGTTCGACAGCGGGTTGCTGCACC
GGCCCGAGGCGGTGACCACCAAAACCGGCCGGCCCTTCCGGGTGTTCACGCCGTTTTGGAAAACCCTCTCGGCCACGCTG
GATCCCGGACTGCCTTTGGCCGCGCCCGACAGCTTCCCGCCGGTGGACGATCCGCCGCCGGCCAGCGACTCCCTGGCCGA
TTGGTCGCTGGAGCCGACCACGCCCGATTGGGCCGGCGGGCTGCGCGATACTTGGCAACCGGGGGAAGCCGGCGCCCAGG
CCCGCCTTGAGGCCTTTTTTAACGGACCGGTCGCCGATTACGCCAGCGGCCGCGACCGCCCGGACAAGCCGCTGACCTCG
AAGCTGTCGCCCCATCTGCGCTTTGGCGAAATCTCCCCCCGTCAGGTGTGGACGGCGGCCCGCCATCACCCCCCCGGTGG
CGGCATGGACGCCTTTTTGCGCGAATTGGGCTGGCGCGAGTTCTGCCATCACCTGCTGTTCCAGGCGCCGACCATGGCCG
AAGAGCCGCTCAAAGCCGAATTCCTGGCTTTCCCCTGGACCGAAGCCAAAGAGATCGGCCCGGCTTTGGCGGCATGGCAA
AGCGGCGAGACCGGCTATCCGATCGTCGATGCCGGCATGCGCGAGCTGTGGCACACCGGGTGGATGCACAACCGGGTGCG
GATGATCGCCGCCTCGTTCCTGATCAAGGATCTACTGATCCCCTGGCAGCACGGCGAGCGCTGGTTCTGGGATACCCTGG
TCGACGCCTGCCCGGCCAATAACGCCGCCGGCTGGCAATGGGTGGCGGGCTGCGGCGCCGATGCCGCGCCGTTCTTCCGC
ATCTTCAATCCGGTGACCCAGGGCGAGCGCTTCGACCCCGAAGGCGCCTATGTCCGGCGCTGGCTGCCCGAGCTGGCCGA
TCTGCCGAGCTCGCTGATCCATCGCCCCTGGGAGGCACCGCCGTTGATCTTGCGCCAAGCCGGAGTGACCTTGGGTAAAA
CCTACCCGCCCCCCCTGATCGACCACGCCCACGCCCGCGTCCGGGCGCTGGAGGCCTTGGCGGTGGTGAACCGCTAG

Upstream 100 bases:

>100_bases
TTTTCCGTTTAAAACCTTAAATCGTTAAAGCGAGATCAAGGTCTTCGCGCTACATGAATCTGGTTTAACGCCGTTTGATC
TGGTTTGCCGGAGAACCCCT

Downstream 100 bases:

>100_bases
GGCAAATCCCGAGCACTCTGGACCAGAGCGCGACGACGATTTGCTCCCCCATCAGAATTCCAGAATGGCCGAAGCCGGGA
TCAATCAGGTGCCGGATCGG

Product: deoxyribodipyrimidine photo-lyase type I

Products: NA

Alternate protein names: DNA photolyase; Photoreactivating enzyme [H]

Number of amino acids: Translated: 478; Mature: 477

Protein sequence:

>478_residues
MTIPVLVWFRDDLRLADNPALCAALDSGAPVIPFYLLDDSTSDGHRPLGGATRWWLGRSLTSLAESIAQRGGRLVLRRGE
ALSAVPELAAETDAAALFANRLYGMDSLRDDDLFMVLRVAGLTVRLFDSGLLHRPEAVTTKTGRPFRVFTPFWKTLSATL
DPGLPLAAPDSFPPVDDPPPASDSLADWSLEPTTPDWAGGLRDTWQPGEAGAQARLEAFFNGPVADYASGRDRPDKPLTS
KLSPHLRFGEISPRQVWTAARHHPPGGGMDAFLRELGWREFCHHLLFQAPTMAEEPLKAEFLAFPWTEAKEIGPALAAWQ
SGETGYPIVDAGMRELWHTGWMHNRVRMIAASFLIKDLLIPWQHGERWFWDTLVDACPANNAAGWQWVAGCGADAAPFFR
IFNPVTQGERFDPEGAYVRRWLPELADLPSSLIHRPWEAPPLILRQAGVTLGKTYPPPLIDHAHARVRALEALAVVNR

Sequences:

>Translated_478_residues
MTIPVLVWFRDDLRLADNPALCAALDSGAPVIPFYLLDDSTSDGHRPLGGATRWWLGRSLTSLAESIAQRGGRLVLRRGE
ALSAVPELAAETDAAALFANRLYGMDSLRDDDLFMVLRVAGLTVRLFDSGLLHRPEAVTTKTGRPFRVFTPFWKTLSATL
DPGLPLAAPDSFPPVDDPPPASDSLADWSLEPTTPDWAGGLRDTWQPGEAGAQARLEAFFNGPVADYASGRDRPDKPLTS
KLSPHLRFGEISPRQVWTAARHHPPGGGMDAFLRELGWREFCHHLLFQAPTMAEEPLKAEFLAFPWTEAKEIGPALAAWQ
SGETGYPIVDAGMRELWHTGWMHNRVRMIAASFLIKDLLIPWQHGERWFWDTLVDACPANNAAGWQWVAGCGADAAPFFR
IFNPVTQGERFDPEGAYVRRWLPELADLPSSLIHRPWEAPPLILRQAGVTLGKTYPPPLIDHAHARVRALEALAVVNR
>Mature_477_residues
TIPVLVWFRDDLRLADNPALCAALDSGAPVIPFYLLDDSTSDGHRPLGGATRWWLGRSLTSLAESIAQRGGRLVLRRGEA
LSAVPELAAETDAAALFANRLYGMDSLRDDDLFMVLRVAGLTVRLFDSGLLHRPEAVTTKTGRPFRVFTPFWKTLSATLD
PGLPLAAPDSFPPVDDPPPASDSLADWSLEPTTPDWAGGLRDTWQPGEAGAQARLEAFFNGPVADYASGRDRPDKPLTSK
LSPHLRFGEISPRQVWTAARHHPPGGGMDAFLRELGWREFCHHLLFQAPTMAEEPLKAEFLAFPWTEAKEIGPALAAWQS
GETGYPIVDAGMRELWHTGWMHNRVRMIAASFLIKDLLIPWQHGERWFWDTLVDACPANNAAGWQWVAGCGADAAPFFRI
FNPVTQGERFDPEGAYVRRWLPELADLPSSLIHRPWEAPPLILRQAGVTLGKTYPPPLIDHAHARVRALEALAVVNR

Specific function: Involved in repair of UV radiation-induced DNA damage. Catalyzes the light-dependent monomerization (300-600 nm) of cyclobutyl pyrimidine dimers (in cis-syn configuration), which are formed between adjacent bases on the same DNA strand upon exposure to ul

COG id: COG0415

COG function: function code L; Deoxyribodipyrimidine photolyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DNA photolyase domain [H]

Homologues:

Organism=Homo sapiens, GI188536100, Length=491, Percent_Identity=30.9572301425662, Blast_Score=178, Evalue=8e-45,
Organism=Homo sapiens, GI4758072, Length=500, Percent_Identity=29.8, Blast_Score=175, Evalue=9e-44,
Organism=Homo sapiens, GI188536103, Length=451, Percent_Identity=29.9334811529933, Blast_Score=160, Evalue=3e-39,
Organism=Escherichia coli, GI1786926, Length=485, Percent_Identity=38.5567010309278, Blast_Score=281, Evalue=5e-77,
Organism=Saccharomyces cerevisiae, GI6324962, Length=512, Percent_Identity=29.4921875, Blast_Score=195, Evalue=1e-50,
Organism=Drosophila melanogaster, GI17137248, Length=503, Percent_Identity=28.6282306163022, Blast_Score=174, Evalue=1e-43,
Organism=Drosophila melanogaster, GI24585455, Length=503, Percent_Identity=28.6282306163022, Blast_Score=174, Evalue=1e-43,
Organism=Drosophila melanogaster, GI24648152, Length=518, Percent_Identity=27.027027027027, Blast_Score=149, Evalue=3e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002081
- InterPro:   IPR018394
- InterPro:   IPR006050
- InterPro:   IPR005101
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00875 DNA_photolyase; PF03441 FAD_binding_7 [H]

EC number: =4.1.99.3 [H]

Molecular weight: Translated: 52865; Mature: 52733

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: PS00141 ASP_PROTEASE ; PS00394 DNA_PHOTOLYASES_1_1 ; PS00691 DNA_PHOTOLYASES_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIPVLVWFRDDLRLADNPALCAALDSGAPVIPFYLLDDSTSDGHRPLGGATRWWLGRSL
CCCEEEEEEECCCEECCCCCEEEEECCCCCEEEEEEEECCCCCCCCCCCCCHHHHHHHHH
TSLAESIAQRGGRLVLRRGEALSAVPELAAETDAAALFANRLYGMDSLRDDDLFMVLRVA
HHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
GLTVRLFDSGLLHRPEAVTTKTGRPFRVFTPFWKTLSATLDPGLPLAAPDSFPPVDDPPP
CCEEEEECCCCCCCCCCCCCCCCCCEEEECHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
ASDSLADWSLEPTTPDWAGGLRDTWQPGEAGAQARLEAFFNGPVADYASGRDRPDKPLTS
CCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHCCCCCCCCCCCHH
KLSPHLRFGEISPRQVWTAARHHPPGGGMDAFLRELGWREFCHHLLFQAPTMAEEPLKAE
HCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHCHHHHHHHHHHCCCCCCCCCCCHH
FLAFPWTEAKEIGPALAAWQSGETGYPIVDAGMRELWHTGWMHNRVRMIAASFLIKDLLI
EEECCCCCHHHHCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PWQHGERWFWDTLVDACPANNAAGWQWVAGCGADAAPFFRIFNPVTQGERFDPEGAYVRR
CCCCCCCHHHHHHHHHCCCCCCCCCEEEECCCCCHHHHHHHHHHHHCCCCCCCCHHHHHH
WLPELADLPSSLIHRPWEAPPLILRQAGVTLGKTYPPPLIDHAHARVRALEALAVVNR
HHHHHHHCCHHHHCCCCCCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TIPVLVWFRDDLRLADNPALCAALDSGAPVIPFYLLDDSTSDGHRPLGGATRWWLGRSL
CCEEEEEEECCCEECCCCCEEEEECCCCCEEEEEEEECCCCCCCCCCCCCHHHHHHHHH
TSLAESIAQRGGRLVLRRGEALSAVPELAAETDAAALFANRLYGMDSLRDDDLFMVLRVA
HHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
GLTVRLFDSGLLHRPEAVTTKTGRPFRVFTPFWKTLSATLDPGLPLAAPDSFPPVDDPPP
CCEEEEECCCCCCCCCCCCCCCCCCEEEECHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
ASDSLADWSLEPTTPDWAGGLRDTWQPGEAGAQARLEAFFNGPVADYASGRDRPDKPLTS
CCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHCCCCCCCCCCCHH
KLSPHLRFGEISPRQVWTAARHHPPGGGMDAFLRELGWREFCHHLLFQAPTMAEEPLKAE
HCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHCHHHHHHHHHHCCCCCCCCCCCHH
FLAFPWTEAKEIGPALAAWQSGETGYPIVDAGMRELWHTGWMHNRVRMIAASFLIKDLLI
EEECCCCCHHHHCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PWQHGERWFWDTLVDACPANNAAGWQWVAGCGADAAPFFRIFNPVTQGERFDPEGAYVRR
CCCCCCCHHHHHHHHHCCCCCCCCCEEEECCCCCHHHHHHHHHHHHCCCCCCCCHHHHHH
WLPELADLPSSLIHRPWEAPPLILRQAGVTLGKTYPPPLIDHAHARVRALEALAVVNR
HHHHHHHCCHHHHCCCCCCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6325460; 8905232; 9278503; 2200511; 7604260 [H]