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The map label for this gene is tuf1 [H]

Identifier: 83594034

GI number: 83594034

Start: 3125604

End: 3126794

Strand: Reverse

Name: tuf1 [H]

Synonym: Rru_A2702

Alternate gene names: 83594034

Gene position: 3126794-3125604 (Counterclockwise)

Preceding gene: 83594039

Following gene: 83594033

Centisome position: 71.83

GC content: 62.64

Gene sequence:

>1191_bases
ATGTCGAAAGAAAAGTTTGCGCGGACGAAGCCCCATTGCAACGTCGGAACGATTGGTCACGTTGACCATGGCAAGACGTC
GCTGACGGCTGCGATCACGAAGGTTCTGGCCGAAGCGGGCGGGGCGACGTTCCAGGCCTACGACCAGATCGACAAGGCGC
CGGAAGAGCGTGCGCGCGGCATCACGATTTCGACGGCGCACGTTGAGTACGAGACGGAAGCGCGTCACTACGCCCACGTC
GACTGCCCGGGCCACGCCGACTATGTGAAGAACATGATCACCGGCGCGGCGCAGATGGACGGGGCGATCCTGGTGGTTTC
GGCCGCCGACGGCCCGATGCCGCAGACCCGCGAGCACATTCTTCTGGCCCGTCAGGTCGGCGTTCCGGCCCTGGTGGTGT
TCCTGAACAAGTGCGACATGGTCGACGACGAGGAGCTGCTTGAGCTGGTCGAGCTTGAGGTCCGCGAGTTGCTGACGAGC
TACGACTTCCCCGGCGATGACATTCCCATCATCAAGGGCTCGGCTCTGGCCGCTCTGGAAGACAGCGACCAGAAGCTTGG
TCACGATGCCATCCTCGAGCTGATGAAGGCGGTGGACGATTACATTCCGCAGCCCGAGCGCCCGAAGGACAAGCCGTTCC
TGATGCCGATCGAAGACGTGTTCTCGATCTCGGGTCGTGGTACGGTGGTGACCGGTCGCGTCGAGCGCGGCATCGTCAAG
GTTGGCGAGGAAATCGAGATCATCGGTATCCGCGACACCCAGAAGACGACCTGCACCGGCGTCGAGATGTTCCGCAAGCT
GCTTGATCAGGGCGAGGCCGGCGACAACATCGGCGCGCTGCTGCGCGGCACCAAGCGTGATGACGTCGAGCGCGGTCAGG
TTCTGGCCAAGCCGGGTTCGATCACCCCGCACACCAAGTTCAAGTGCGAGGCCTATATCCTGACGAAGGAAGAGGGTGGC
CGTCACACGCCGTTCTTCTCCAACTATCGTCCGCAGTTCTATTTCCGCACGACCGACGTGACCGGGACGATCGAGCTGCC
GGAAGGCACCGAGATGGTTATGCCCGGTGACAACATCGGCATGACGGTACAGCTGATCGCGCCGATTGCCATGGACGAGG
GTCTGCGGTTCGCTATCCGCGAGGGTGGCCGTACCGTCGGTGCCGGCGTCGTCGCCAGCATCGTTCAGTAA

Upstream 100 bases:

>100_bases
CGCTCCATTCCGTTCCAAGGCCCCTACGTTTAACGCCGCCCTCTCAGAAGCCGTTCGGTCGGGTCGGGCAACCGCAACCA
TAGAAGGTACGGGATCGAAG

Downstream 100 bases:

>100_bases
GACGCCTAAGCCATCCAGCCAGTCCTTGTCGGGTCTGGCTGGATGGCGCGTGCCTTGGGGCTGGGCAACCGGGCGCGGCT
AGCGGATGGATCAAGAAAGT

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu 1 [H]

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MSKEKFARTKPHCNVGTIGHVDHGKTSLTAAITKVLAEAGGATFQAYDQIDKAPEERARGITISTAHVEYETEARHYAHV
DCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLARQVGVPALVVFLNKCDMVDDEELLELVELEVRELLTS
YDFPGDDIPIIKGSALAALEDSDQKLGHDAILELMKAVDDYIPQPERPKDKPFLMPIEDVFSISGRGTVVTGRVERGIVK
VGEEIEIIGIRDTQKTTCTGVEMFRKLLDQGEAGDNIGALLRGTKRDDVERGQVLAKPGSITPHTKFKCEAYILTKEEGG
RHTPFFSNYRPQFYFRTTDVTGTIELPEGTEMVMPGDNIGMTVQLIAPIAMDEGLRFAIREGGRTVGAGVVASIVQ

Sequences:

>Translated_396_residues
MSKEKFARTKPHCNVGTIGHVDHGKTSLTAAITKVLAEAGGATFQAYDQIDKAPEERARGITISTAHVEYETEARHYAHV
DCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLARQVGVPALVVFLNKCDMVDDEELLELVELEVRELLTS
YDFPGDDIPIIKGSALAALEDSDQKLGHDAILELMKAVDDYIPQPERPKDKPFLMPIEDVFSISGRGTVVTGRVERGIVK
VGEEIEIIGIRDTQKTTCTGVEMFRKLLDQGEAGDNIGALLRGTKRDDVERGQVLAKPGSITPHTKFKCEAYILTKEEGG
RHTPFFSNYRPQFYFRTTDVTGTIELPEGTEMVMPGDNIGMTVQLIAPIAMDEGLRFAIREGGRTVGAGVVASIVQ
>Mature_395_residues
SKEKFARTKPHCNVGTIGHVDHGKTSLTAAITKVLAEAGGATFQAYDQIDKAPEERARGITISTAHVEYETEARHYAHVD
CPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLARQVGVPALVVFLNKCDMVDDEELLELVELEVRELLTSY
DFPGDDIPIIKGSALAALEDSDQKLGHDAILELMKAVDDYIPQPERPKDKPFLMPIEDVFSISGRGTVVTGRVERGIVKV
GEEIEIIGIRDTQKTTCTGVEMFRKLLDQGEAGDNIGALLRGTKRDDVERGQVLAKPGSITPHTKFKCEAYILTKEEGGR
HTPFFSNYRPQFYFRTTDVTGTIELPEGTEMVMPGDNIGMTVQLIAPIAMDEGLRFAIREGGRTVGAGVVASIVQ

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis [H]

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=394, Percent_Identity=58.6294416243655, Blast_Score=493, Evalue=1e-139,
Organism=Homo sapiens, GI4503475, Length=444, Percent_Identity=28.8288288288288, Blast_Score=162, Evalue=3e-40,
Organism=Homo sapiens, GI4503471, Length=450, Percent_Identity=28.4444444444444, Blast_Score=162, Evalue=6e-40,
Organism=Homo sapiens, GI223555963, Length=439, Percent_Identity=28.9293849658314, Blast_Score=159, Evalue=4e-39,
Organism=Homo sapiens, GI5729864, Length=439, Percent_Identity=28.9293849658314, Blast_Score=159, Evalue=5e-39,
Organism=Homo sapiens, GI194018522, Length=328, Percent_Identity=29.5731707317073, Blast_Score=119, Evalue=4e-27,
Organism=Homo sapiens, GI194018520, Length=328, Percent_Identity=29.5731707317073, Blast_Score=119, Evalue=5e-27,
Organism=Homo sapiens, GI194097354, Length=328, Percent_Identity=29.5731707317073, Blast_Score=119, Evalue=5e-27,
Organism=Homo sapiens, GI46094014, Length=329, Percent_Identity=29.483282674772, Blast_Score=118, Evalue=9e-27,
Organism=Homo sapiens, GI54607086, Length=321, Percent_Identity=28.0373831775701, Blast_Score=115, Evalue=6e-26,
Organism=Homo sapiens, GI4503507, Length=295, Percent_Identity=28.135593220339, Blast_Score=79, Evalue=7e-15,
Organism=Homo sapiens, GI157426893, Length=285, Percent_Identity=27.0175438596491, Blast_Score=75, Evalue=8e-14,
Organism=Escherichia coli, GI1790412, Length=396, Percent_Identity=78.5353535353535, Blast_Score=620, Evalue=1e-179,
Organism=Escherichia coli, GI1789737, Length=395, Percent_Identity=78.7341772151899, Blast_Score=619, Evalue=1e-179,
Organism=Escherichia coli, GI2367247, Length=379, Percent_Identity=29.287598944591, Blast_Score=135, Evalue=6e-33,
Organism=Escherichia coli, GI48994988, Length=272, Percent_Identity=31.9852941176471, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI1789108, Length=271, Percent_Identity=29.8892988929889, Blast_Score=87, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17556456, Length=399, Percent_Identity=51.8796992481203, Blast_Score=397, Evalue=1e-111,
Organism=Caenorhabditis elegans, GI25141371, Length=387, Percent_Identity=43.6692506459948, Blast_Score=338, Evalue=3e-93,
Organism=Caenorhabditis elegans, GI17552884, Length=451, Percent_Identity=29.2682926829268, Blast_Score=159, Evalue=3e-39,
Organism=Caenorhabditis elegans, GI17569207, Length=451, Percent_Identity=29.2682926829268, Blast_Score=159, Evalue=3e-39,
Organism=Caenorhabditis elegans, GI32566303, Length=432, Percent_Identity=26.8518518518519, Blast_Score=136, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI115532067, Length=430, Percent_Identity=28.1395348837209, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI115532065, Length=430, Percent_Identity=28.1395348837209, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17506081, Length=298, Percent_Identity=29.5302013422819, Blast_Score=124, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI32566629, Length=355, Percent_Identity=27.6056338028169, Blast_Score=120, Evalue=8e-28,
Organism=Caenorhabditis elegans, GI32566301, Length=149, Percent_Identity=38.255033557047, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17509919, Length=274, Percent_Identity=27.7372262773723, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17557151, Length=234, Percent_Identity=28.6324786324786, Blast_Score=76, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6324761, Length=396, Percent_Identity=65.9090909090909, Blast_Score=533, Evalue=1e-152,
Organism=Saccharomyces cerevisiae, GI6325337, Length=442, Percent_Identity=29.185520361991, Blast_Score=161, Evalue=1e-40,
Organism=Saccharomyces cerevisiae, GI6319594, Length=442, Percent_Identity=29.185520361991, Blast_Score=161, Evalue=1e-40,
Organism=Saccharomyces cerevisiae, GI6322937, Length=361, Percent_Identity=26.3157894736842, Blast_Score=102, Evalue=7e-23,
Organism=Saccharomyces cerevisiae, GI6320377, Length=345, Percent_Identity=28.4057971014493, Blast_Score=91, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6320863, Length=270, Percent_Identity=27.7777777777778, Blast_Score=79, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6323320, Length=242, Percent_Identity=27.6859504132231, Blast_Score=71, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6323098, Length=178, Percent_Identity=28.6516853932584, Blast_Score=63, Evalue=9e-11,
Organism=Drosophila melanogaster, GI281363316, Length=393, Percent_Identity=58.2697201017812, Blast_Score=491, Evalue=1e-139,
Organism=Drosophila melanogaster, GI17864358, Length=393, Percent_Identity=58.2697201017812, Blast_Score=491, Evalue=1e-139,
Organism=Drosophila melanogaster, GI19921738, Length=384, Percent_Identity=49.7395833333333, Blast_Score=402, Evalue=1e-112,
Organism=Drosophila melanogaster, GI45553807, Length=455, Percent_Identity=28.1318681318681, Blast_Score=157, Evalue=1e-38,
Organism=Drosophila melanogaster, GI45553816, Length=455, Percent_Identity=28.1318681318681, Blast_Score=157, Evalue=1e-38,
Organism=Drosophila melanogaster, GI24651721, Length=455, Percent_Identity=28.1318681318681, Blast_Score=157, Evalue=1e-38,
Organism=Drosophila melanogaster, GI17864154, Length=455, Percent_Identity=28.1318681318681, Blast_Score=157, Evalue=1e-38,
Organism=Drosophila melanogaster, GI24652838, Length=455, Percent_Identity=27.9120879120879, Blast_Score=152, Evalue=3e-37,
Organism=Drosophila melanogaster, GI17137572, Length=455, Percent_Identity=27.9120879120879, Blast_Score=152, Evalue=3e-37,
Organism=Drosophila melanogaster, GI19922690, Length=303, Percent_Identity=30.6930693069307, Blast_Score=129, Evalue=4e-30,
Organism=Drosophila melanogaster, GI45550900, Length=340, Percent_Identity=27.9411764705882, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI17137380, Length=323, Percent_Identity=30.6501547987616, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24647046, Length=293, Percent_Identity=25.938566552901, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24647048, Length=293, Percent_Identity=25.938566552901, Blast_Score=79, Evalue=5e-15,
Organism=Drosophila melanogaster, GI78706572, Length=257, Percent_Identity=26.4591439688716, Blast_Score=66, Evalue=3e-11,
Organism=Drosophila melanogaster, GI28572034, Length=164, Percent_Identity=35.3658536585366, Blast_Score=65, Evalue=7e-11,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 43188; Mature: 43057

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKEKFARTKPHCNVGTIGHVDHGKTSLTAAITKVLAEAGGATFQAYDQIDKAPEERARG
CCCHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEHHHHHHHCCHHHHHCC
ITISTAHVEYETEARHYAHVDCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHI
EEEEEEEEEEECCCCEEEEECCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHH
LLARQVGVPALVVFLNKCDMVDDEELLELVELEVRELLTSYDFPGDDIPIIKGSALAALE
HHHHHCCCCEEEHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCEEEEEC
DSDQKLGHDAILELMKAVDDYIPQPERPKDKPFLMPIEDVFSISGRGTVVTGRVERGIVK
CCCHHCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEHHHHHCCCCCCEEEECHHHHCHHH
VGEEIEIIGIRDTQKTTCTGVEMFRKLLDQGEAGDNIGALLRGTKRDDVERGQVLAKPGS
CCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCCCEEEECCCC
ITPHTKFKCEAYILTKEEGGRHTPFFSNYRPQFYFRTTDVTGTIELPEGTEMVMPGDNIG
CCCCCCEEEEEEEEEECCCCCCCCCCCCCCCEEEEEEECCEEEEECCCCCEEEECCCCCC
MTVQLIAPIAMDEGLRFAIREGGRTVGAGVVASIVQ
EEEEEEEEHHHCCCCEEEECCCCCEEHHHHHHHHCC
>Mature Secondary Structure 
SKEKFARTKPHCNVGTIGHVDHGKTSLTAAITKVLAEAGGATFQAYDQIDKAPEERARG
CCHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEHHHHHHHCCHHHHHCC
ITISTAHVEYETEARHYAHVDCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHI
EEEEEEEEEEECCCCEEEEECCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHH
LLARQVGVPALVVFLNKCDMVDDEELLELVELEVRELLTSYDFPGDDIPIIKGSALAALE
HHHHHCCCCEEEHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCEEEEEC
DSDQKLGHDAILELMKAVDDYIPQPERPKDKPFLMPIEDVFSISGRGTVVTGRVERGIVK
CCCHHCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEHHHHHCCCCCCEEEECHHHHCHHH
VGEEIEIIGIRDTQKTTCTGVEMFRKLLDQGEAGDNIGALLRGTKRDDVERGQVLAKPGS
CCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCCCEEEECCCC
ITPHTKFKCEAYILTKEEGGRHTPFFSNYRPQFYFRTTDVTGTIELPEGTEMVMPGDNIG
CCCCCCEEEEEEEEEECCCCCCCCCCCCCCCEEEEEEECCEEEEECCCCCEEEECCCCCC
MTVQLIAPIAMDEGLRFAIREGGRTVGAGVVASIVQ
EEEEEEEEHHHCCCCEEEECCCCCEEHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA