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The map label for this gene is ybfF [H]

Identifier: 83594039

GI number: 83594039

Start: 3130000

End: 3130794

Strand: Reverse

Name: ybfF [H]

Synonym: Rru_A2707

Alternate gene names: 83594039

Gene position: 3130794-3130000 (Counterclockwise)

Preceding gene: 83594040

Following gene: 83594034

Centisome position: 71.93

GC content: 69.56

Gene sequence:

>795_bases
ATGACCGTGGATCTCGCCGCCCAATGCCTGGGAGAGGGGCCGCCGCTGGTGGTGCTCCACGGCCTGTTCGGATCGGCGCG
CAATTGGGCGGGAATCGCCCGCCGGCTGGGCGATCGCTATCGGGTCCATGCGCTTGATCTGCGCAATCACGGCGAGTCGC
CCTGGACCGAGGCCCTGGATTACCCGCTGATGGCCGGCGATGTGGCGGCCTATATCGAGCGCGAGATCGGCGACGGCCCC
GCCCCGGTGGTGGTGGGCCATAGCATGGGCGGCAAGGTGGCGATGACCCTGGCCCTGCTTCACCCCGGGCGGGTTGGCGC
CCTGGTGGTGGCCGATATCGCCCCGGTGGCCTATCGGCCGGGCCTGGACGCCTTCGCCGAGGCCATGCTGGCCGTGCCGC
TGGCCGGACTCGCCCGCCGCTCCCAGGTCGAGGCCCTGCTTGCCGACAGCATCCCTTCCCCGGGCATCCGGCGTTTTCTG
GCGCAAAATATCGTTGAGGACCCGGCGGGCGGCTTGCGTTGGCGCTTGAACCTCGAAGGTCTGCGCCGTGAAATGGCCAC
CCTGGCCGGCTTTCCGGCCTTTCCCGCCGATCGGACTTTCAGCGGGCGCACCCTGGTGGTGCGCGGCGCCCTGTCGGATT
ATGTCGGCGAGGCGGAGCGGCCGGCCTTTGAGCGGCTGTTTCCGGGCTATCGTCTGGCCACCCTCAAGGGCGCCGGCCAT
TGGCTGCATTCGGAAAAGCCCGAGGCCTTCGTCGATATCCTGCGCGGCTTTCTTGGCGCGCCCGCCGCCCCTTAA

Upstream 100 bases:

>100_bases
CACCGCCCTGTGGGTCAATAAGCAAAACACCCTCAAGGTCTGCGCCCGCGATGGCTGCTATGGCGGCCAGACCCGGAAAA
CCGGCAAAAAATAGACGCCG

Downstream 100 bases:

>100_bases
AACGCCCCCGGACCATCGGGGCGGCAGCCGGCTTCCATCATCAACACCATCCAGGGGCGCCAGCGCAGGCGGTCGTCGAA
GCGCTGGCCGTTGGGGCCGG

Product: alpha/beta hydrolase fold

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 264; Mature: 263

Protein sequence:

>264_residues
MTVDLAAQCLGEGPPLVVLHGLFGSARNWAGIARRLGDRYRVHALDLRNHGESPWTEALDYPLMAGDVAAYIEREIGDGP
APVVVGHSMGGKVAMTLALLHPGRVGALVVADIAPVAYRPGLDAFAEAMLAVPLAGLARRSQVEALLADSIPSPGIRRFL
AQNIVEDPAGGLRWRLNLEGLRREMATLAGFPAFPADRTFSGRTLVVRGALSDYVGEAERPAFERLFPGYRLATLKGAGH
WLHSEKPEAFVDILRGFLGAPAAP

Sequences:

>Translated_264_residues
MTVDLAAQCLGEGPPLVVLHGLFGSARNWAGIARRLGDRYRVHALDLRNHGESPWTEALDYPLMAGDVAAYIEREIGDGP
APVVVGHSMGGKVAMTLALLHPGRVGALVVADIAPVAYRPGLDAFAEAMLAVPLAGLARRSQVEALLADSIPSPGIRRFL
AQNIVEDPAGGLRWRLNLEGLRREMATLAGFPAFPADRTFSGRTLVVRGALSDYVGEAERPAFERLFPGYRLATLKGAGH
WLHSEKPEAFVDILRGFLGAPAAP
>Mature_263_residues
TVDLAAQCLGEGPPLVVLHGLFGSARNWAGIARRLGDRYRVHALDLRNHGESPWTEALDYPLMAGDVAAYIEREIGDGPA
PVVVGHSMGGKVAMTLALLHPGRVGALVVADIAPVAYRPGLDAFAEAMLAVPLAGLARRSQVEALLADSIPSPGIRRFLA
QNIVEDPAGGLRWRLNLEGLRREMATLAGFPAFPADRTFSGRTLVVRGALSDYVGEAERPAFERLFPGYRLATLKGAGHW
LHSEKPEAFVDILRGFLGAPAAP

Specific function: Displays esterase activity toward palmitoyl-CoA, malonyl-CoA and pNP-butyrate [H]

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dmpD/todF/xylF esterase family [H]

Homologues:

Organism=Homo sapiens, GI23200012, Length=254, Percent_Identity=38.1889763779528, Blast_Score=150, Evalue=9e-37,
Organism=Homo sapiens, GI23200008, Length=254, Percent_Identity=38.1889763779528, Blast_Score=150, Evalue=1e-36,
Organism=Homo sapiens, GI223941839, Length=247, Percent_Identity=32.3886639676113, Blast_Score=115, Evalue=4e-26,
Organism=Escherichia coli, GI1786902, Length=256, Percent_Identity=34.375, Blast_Score=136, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI17508535, Length=258, Percent_Identity=32.5581395348837, Blast_Score=151, Evalue=4e-37,
Organism=Caenorhabditis elegans, GI17507063, Length=258, Percent_Identity=29.0697674418605, Blast_Score=114, Evalue=4e-26,
Organism=Saccharomyces cerevisiae, GI6321468, Length=265, Percent_Identity=33.5849056603774, Blast_Score=122, Evalue=6e-29,
Organism=Drosophila melanogaster, GI24640348, Length=266, Percent_Identity=30.0751879699248, Blast_Score=121, Evalue=5e-28,
Organism=Drosophila melanogaster, GI24646055, Length=265, Percent_Identity=27.1698113207547, Blast_Score=86, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: 3.1.-.- [C]

Molecular weight: Translated: 28195; Mature: 28064

Theoretical pI: Translated: 7.24; Mature: 7.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVDLAAQCLGEGPPLVVLHGLFGSARNWAGIARRLGDRYRVHALDLRNHGESPWTEALD
CCCCHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHC
YPLMAGDVAAYIEREIGDGPAPVVVGHSMGGKVAMTLALLHPGRVGALVVADIAPVAYRP
CCCHHHHHHHHHHHHCCCCCCCEEEECCCCCCEEEEEEECCCCCCCEEEEEHHCHHHHCC
GLDAFAEAMLAVPLAGLARRSQVEALLADSIPSPGIRRFLAQNIVEDPAGGLRWRLNLEG
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCEEEEEEHHH
LRREMATLAGFPAFPADRTFSGRTLVVRGALSDYVGEAERPAFERLFPGYRLATLKGAGH
HHHHHHHHHCCCCCCCCCCCCCCEEEEECCHHHHHCCCCCCHHHHHCCCCEEEEECCCCC
WLHSEKPEAFVDILRGFLGAPAAP
CCCCCCCHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
TVDLAAQCLGEGPPLVVLHGLFGSARNWAGIARRLGDRYRVHALDLRNHGESPWTEALD
CCCHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHC
YPLMAGDVAAYIEREIGDGPAPVVVGHSMGGKVAMTLALLHPGRVGALVVADIAPVAYRP
CCCHHHHHHHHHHHHCCCCCCCEEEECCCCCCEEEEEEECCCCCCCEEEEEHHCHHHHCC
GLDAFAEAMLAVPLAGLARRSQVEALLADSIPSPGIRRFLAQNIVEDPAGGLRWRLNLEG
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCEEEEEEHHH
LRREMATLAGFPAFPADRTFSGRTLVVRGALSDYVGEAERPAFERLFPGYRLATLKGAGH
HHHHHHHHHCCCCCCCCCCCCCCEEEEECCHHHHHCCCCCCHHHHHCCCCEEEEECCCCC
WLHSEKPEAFVDILRGFLGAPAAP
CCCCCCCHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 10493123 [H]