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The map label for this gene is ykoW [H]

Identifier: 83593936

GI number: 83593936

Start: 3027678

End: 3029714

Strand: Reverse

Name: ykoW [H]

Synonym: Rru_A2604

Alternate gene names: 83593936

Gene position: 3029714-3027678 (Counterclockwise)

Preceding gene: 83593937

Following gene: 83593933

Centisome position: 69.6

GC content: 62.64

Gene sequence:

>2037_bases
GTGCTCGGAAAATTTCTCGCCGTTCAGCAGGCTTTGCGGGTCTCCCTGCTTAGCCTTGCGGTGTCTTTGGGATTTCTGGG
CATGCAAACGGTCTGGCTGGCCAGCGCCGCCGACCGCGATTATCTGATCTTGCGCGATCGGACGGCCTCGTCCTTGCAAT
CGGGGTTGTCGAACGCCCTGTGGAATTTCGATGCGGAAACCGCCAAGGCCATCATCAGCGATGCGCTGCGCGCCAATCCC
ATTCGCTCCATTCACGTCATCGATTCCAATGGCCGGGTTTTCGCCGATGGCCAAAGCGCCCAGCCGGTCGTCGCGCCCTC
CCTATGGGAAAGCTATCTGGGTTGGCTGGTCGATCTGAAAAGCGAGATGACCCTTGATCTGCGCTTTTCGAGCCCGACCG
ACGACCATTACCAGGGGATGGTCGTCGGCTTCGTCTTTATCACCTTCGATAAGGACCATTTCATCCGGGGGATCGCCGAT
CGACTGGCCGATCAGGTGCTTGGCATTCTGGTCATCGTGCTGGTGGTGGCCCTGACCACCAGCATCGTCTTCCACCTGTT
CATCTCGCGGCCGATCATCCGCGTTGCCCGCGGCGTCGCCGATGTTGATCCCGAAGATCCCGCCGCCACGGTTTTGACCG
TTCCCCGCGCCCATCAACGCAATGAAATCGGCCGCATGACCAACCGGCTCAATCATCTGTTGGGCCGGCTGGCCGAGGTG
CAGGGGGAACTGCGGCTGTTGGCCACCCGCGATCCGCTGACCGATCTGCCCAACCGCAGCCTGATCCTTGAGATGATGGA
CGCGACGATCAACCGGGTCGCCCTGGACGGTGGCCGCTGCGCGGTGATGTTTCTTGATCTCGACATGTTCAAGCACGTCA
ACGACAGCCTGGGACACGAGGCGGGTGATCTCATCCTCAAGGACATCGGACGGCGGTTCGACGCGGTGGTCGCCGGCCTG
GGGGCGGTGGGGCGGTTGGGCGGCGACGAGTTCGTCGTTCTCATTGACCGCTATGAGCGCGAAGACGATCTGGCCCGCAT
CGCCCGCCGGCTGATCACCACGGTCAAACGGCCCATCGAGGTCGATGGCACCCTGGTCCATTCAAGCACCTCGGTCGGCA
TCGCCTGTTATCCGGTCGATGGTGCCACCCCCCAGGGGCTGTTGCGCAATGCCGATACGGCGATGTATGCGGTCAAGGCG
AGCGGGCCGGGCCAATGGGCGTTCTTCGACCGCTCGATGACCGAGAACGCCCTGGTGCGGCTGCGGACCGAGGCGTCCTT
GCGCTCGGCGATCGAAAAGGGCCAATTCGTTTTGCACTATCAGCCCAAGCTTGATCTGCGCTCGAACCGTATCAGCGGCT
GCGAGGCCTTGATCCGCTGGGACTTGAACGGAGATTTGGTGCCGCCGGGCCGCTTCATCTCGATCGCCGAGGAAACCGGC
ATGATCTATGACATCGGGCTATGGGTGCTGCGCGAGGCCTGTCAGGCGCAACGGCGCTGGCGCCGCCACGCCGGCGCGGT
GCCGATCGCCATCAATGTCTCGGTGCGCCAGCTCAAGGACGAGCGGTTCATCGACGATTTCCTCGATATCATCCAGGCGA
GCGGCGCCGATCCCGAACTGCTGGAAGTCGAAGTGACCGAAAGCGCCCTGATGAGCAGCATCGAGGTCAGCGCCCATCTG
CTGCGCGAGATGCGCAGCCATGGCGTTCGCCTGTCGATCGATGACTTTGGCACGGGGTATTCCTCGCTGGCCTATCTGAA
GAACCTGCCCATCGATATCCTCAAGATCGACCGCAGTTTCGTCAGCGATCTGCCCGAGGAAAGCTCGATCCCGCGCATGA
TCATCGGGCTTGCCGGCCAGATGGGACTGCGCACGGTGGCCGAGGGGATCGAGACCGAGGGGCAATTGGCCTGGCTGCGC
GACAATGGCTGCGACATGATCCAGGGCTTCCTGATCGAAAAGCCGCTGCCCGAGGCCGACTTCCTCCGCTTCGTCGACGC
CTATCGGACCGCACGGGCCTTCCCCTCGGCGTCTTGA

Upstream 100 bases:

>100_bases
GTTAGAGTGGGGCTTGTGGTGGCGCCTGATCCGCGTCGCGGGGTCGGTGCGCGCGGTCGGGGGAAATCCAGGGATCTTTG
ACACAGACGGGGGAGAACCG

Downstream 100 bases:

>100_bases
GCGAAAATCTGCGGACCCGTCTCACCCGTGGTCGAGGCCGCGGGCCATGAGCACCGCCTGGGTGCGGTTGCCGGCGTCAA
GCGCCTTCAAGATCCCGGTG

Product: diguanylate cyclase/phosphodiesterase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 678; Mature: 678

Protein sequence:

>678_residues
MLGKFLAVQQALRVSLLSLAVSLGFLGMQTVWLASAADRDYLILRDRTASSLQSGLSNALWNFDAETAKAIISDALRANP
IRSIHVIDSNGRVFADGQSAQPVVAPSLWESYLGWLVDLKSEMTLDLRFSSPTDDHYQGMVVGFVFITFDKDHFIRGIAD
RLADQVLGILVIVLVVALTTSIVFHLFISRPIIRVARGVADVDPEDPAATVLTVPRAHQRNEIGRMTNRLNHLLGRLAEV
QGELRLLATRDPLTDLPNRSLILEMMDATINRVALDGGRCAVMFLDLDMFKHVNDSLGHEAGDLILKDIGRRFDAVVAGL
GAVGRLGGDEFVVLIDRYEREDDLARIARRLITTVKRPIEVDGTLVHSSTSVGIACYPVDGATPQGLLRNADTAMYAVKA
SGPGQWAFFDRSMTENALVRLRTEASLRSAIEKGQFVLHYQPKLDLRSNRISGCEALIRWDLNGDLVPPGRFISIAEETG
MIYDIGLWVLREACQAQRRWRRHAGAVPIAINVSVRQLKDERFIDDFLDIIQASGADPELLEVEVTESALMSSIEVSAHL
LREMRSHGVRLSIDDFGTGYSSLAYLKNLPIDILKIDRSFVSDLPEESSIPRMIIGLAGQMGLRTVAEGIETEGQLAWLR
DNGCDMIQGFLIEKPLPEADFLRFVDAYRTARAFPSAS

Sequences:

>Translated_678_residues
MLGKFLAVQQALRVSLLSLAVSLGFLGMQTVWLASAADRDYLILRDRTASSLQSGLSNALWNFDAETAKAIISDALRANP
IRSIHVIDSNGRVFADGQSAQPVVAPSLWESYLGWLVDLKSEMTLDLRFSSPTDDHYQGMVVGFVFITFDKDHFIRGIAD
RLADQVLGILVIVLVVALTTSIVFHLFISRPIIRVARGVADVDPEDPAATVLTVPRAHQRNEIGRMTNRLNHLLGRLAEV
QGELRLLATRDPLTDLPNRSLILEMMDATINRVALDGGRCAVMFLDLDMFKHVNDSLGHEAGDLILKDIGRRFDAVVAGL
GAVGRLGGDEFVVLIDRYEREDDLARIARRLITTVKRPIEVDGTLVHSSTSVGIACYPVDGATPQGLLRNADTAMYAVKA
SGPGQWAFFDRSMTENALVRLRTEASLRSAIEKGQFVLHYQPKLDLRSNRISGCEALIRWDLNGDLVPPGRFISIAEETG
MIYDIGLWVLREACQAQRRWRRHAGAVPIAINVSVRQLKDERFIDDFLDIIQASGADPELLEVEVTESALMSSIEVSAHL
LREMRSHGVRLSIDDFGTGYSSLAYLKNLPIDILKIDRSFVSDLPEESSIPRMIIGLAGQMGLRTVAEGIETEGQLAWLR
DNGCDMIQGFLIEKPLPEADFLRFVDAYRTARAFPSAS
>Mature_678_residues
MLGKFLAVQQALRVSLLSLAVSLGFLGMQTVWLASAADRDYLILRDRTASSLQSGLSNALWNFDAETAKAIISDALRANP
IRSIHVIDSNGRVFADGQSAQPVVAPSLWESYLGWLVDLKSEMTLDLRFSSPTDDHYQGMVVGFVFITFDKDHFIRGIAD
RLADQVLGILVIVLVVALTTSIVFHLFISRPIIRVARGVADVDPEDPAATVLTVPRAHQRNEIGRMTNRLNHLLGRLAEV
QGELRLLATRDPLTDLPNRSLILEMMDATINRVALDGGRCAVMFLDLDMFKHVNDSLGHEAGDLILKDIGRRFDAVVAGL
GAVGRLGGDEFVVLIDRYEREDDLARIARRLITTVKRPIEVDGTLVHSSTSVGIACYPVDGATPQGLLRNADTAMYAVKA
SGPGQWAFFDRSMTENALVRLRTEASLRSAIEKGQFVLHYQPKLDLRSNRISGCEALIRWDLNGDLVPPGRFISIAEETG
MIYDIGLWVLREACQAQRRWRRHAGAVPIAINVSVRQLKDERFIDDFLDIIQASGADPELLEVEVTESALMSSIEVSAHL
LREMRSHGVRLSIDDFGTGYSSLAYLKNLPIDILKIDRSFVSDLPEESSIPRMIIGLAGQMGLRTVAEGIETEGQLAWLR
DNGCDMIQGFLIEKPLPEADFLRFVDAYRTARAFPSAS

Specific function: Probable signaling protein whose physiological role is not yet known [H]

COG id: COG5001

COG function: function code T; Predicted signal transduction protein containing a membrane domain, an EAL and a GGDEF domain

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1787541, Length=432, Percent_Identity=36.8055555555556, Blast_Score=290, Evalue=3e-79,
Organism=Escherichia coli, GI226510982, Length=529, Percent_Identity=28.5444234404537, Blast_Score=241, Evalue=1e-64,
Organism=Escherichia coli, GI87081921, Length=413, Percent_Identity=34.3825665859564, Blast_Score=217, Evalue=2e-57,
Organism=Escherichia coli, GI87081743, Length=243, Percent_Identity=37.8600823045267, Blast_Score=160, Evalue=2e-40,
Organism=Escherichia coli, GI1790496, Length=250, Percent_Identity=35.2, Blast_Score=154, Evalue=2e-38,
Organism=Escherichia coli, GI1788502, Length=263, Percent_Identity=33.0798479087452, Blast_Score=142, Evalue=5e-35,
Organism=Escherichia coli, GI1787055, Length=287, Percent_Identity=30.6620209059233, Blast_Score=135, Evalue=7e-33,
Organism=Escherichia coli, GI87081845, Length=266, Percent_Identity=32.3308270676692, Blast_Score=131, Evalue=2e-31,
Organism=Escherichia coli, GI87081980, Length=250, Percent_Identity=32, Blast_Score=123, Evalue=4e-29,
Organism=Escherichia coli, GI1786507, Length=257, Percent_Identity=29.1828793774319, Blast_Score=119, Evalue=8e-28,
Organism=Escherichia coli, GI1788849, Length=249, Percent_Identity=32.1285140562249, Blast_Score=111, Evalue=1e-25,
Organism=Escherichia coli, GI87082096, Length=239, Percent_Identity=29.2887029288703, Blast_Score=100, Evalue=6e-22,
Organism=Escherichia coli, GI1788381, Length=426, Percent_Identity=24.4131455399061, Blast_Score=94, Evalue=4e-20,
Organism=Escherichia coli, GI87081977, Length=168, Percent_Identity=31.547619047619, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1788956, Length=167, Percent_Identity=32.3353293413174, Blast_Score=75, Evalue=2e-14,
Organism=Escherichia coli, GI1786584, Length=169, Percent_Identity=28.4023668639053, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI145693134, Length=156, Percent_Identity=30.7692307692308, Blast_Score=69, Evalue=7e-13,
Organism=Escherichia coli, GI1787262, Length=169, Percent_Identity=28.4023668639053, Blast_Score=67, Evalue=4e-12,
Organism=Escherichia coli, GI1787410, Length=222, Percent_Identity=26.5765765765766, Blast_Score=66, Evalue=8e-12,
Organism=Escherichia coli, GI1787802, Length=220, Percent_Identity=27.2727272727273, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR001633
- InterPro:   IPR005330
- InterPro:   IPR000014 [H]

Pfam domain/function: PF00563 EAL; PF00990 GGDEF; PF03707 MHYT [H]

EC number: NA

Molecular weight: Translated: 74940; Mature: 74940

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: PS50885 HAMP ; PS50883 EAL ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLGKFLAVQQALRVSLLSLAVSLGFLGMQTVWLASAADRDYLILRDRTASSLQSGLSNAL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHH
WNFDAETAKAIISDALRANPIRSIHVIDSNGRVFADGQSAQPVVAPSLWESYLGWLVDLK
CCCCHHHHHHHHHHHHHCCCCCEEEEECCCCEEEECCCCCCCCCCHHHHHHHHHHHHHCC
SEMTLDLRFSSPTDDHYQGMVVGFVFITFDKDHFIRGIADRLADQVLGILVIVLVVALTT
CCEEEEEEECCCCCCHHCCEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SIVFHLFISRPIIRVARGVADVDPEDPAATVLTVPRAHQRNEIGRMTNRLNHLLGRLAEV
HHHHHHHHHCHHHHHHHCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHH
QGELRLLATRDPLTDLPNRSLILEMMDATINRVALDGGRCAVMFLDLDMFKHVNDSLGHE
CCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHEEECCCCEEEEEEEHHHHHHHHHHHCCH
AGDLILKDIGRRFDAVVAGLGAVGRLGGDEFVVLIDRYEREDDLARIARRLITTVKRPIE
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCC
VDGTLVHSSTSVGIACYPVDGATPQGLLRNADTAMYAVKASGPGQWAFFDRSMTENALVR
CCCEEEECCCCCCEEEEECCCCCHHHHHCCCCCEEEEEEECCCCCEEEEECCCCCCHHEE
LRTEASLRSAIEKGQFVLHYQPKLDLRSNRISGCEALIRWDLNGDLVPPGRFISIAEETG
EEHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCEEEEEECCCCCCCCCCCEEEEEHHCC
MIYDIGLWVLREACQAQRRWRRHAGAVPIAINVSVRQLKDERFIDDFLDIIQASGADPEL
CEEEHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHHHHHHCCCCCCCE
LEVEVTESALMSSIEVSAHLLREMRSHGVRLSIDDFGTGYSSLAYLKNLPIDILKIDRSF
EEEEEHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHCCCCCEEECCHHH
VSDLPEESSIPRMIIGLAGQMGLRTVAEGIETEGQLAWLRDNGCDMIQGFLIEKPLPEAD
HHCCCCCCCCCHHHHHHCCHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHCCCCCHHH
FLRFVDAYRTARAFPSAS
HHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MLGKFLAVQQALRVSLLSLAVSLGFLGMQTVWLASAADRDYLILRDRTASSLQSGLSNAL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHH
WNFDAETAKAIISDALRANPIRSIHVIDSNGRVFADGQSAQPVVAPSLWESYLGWLVDLK
CCCCHHHHHHHHHHHHHCCCCCEEEEECCCCEEEECCCCCCCCCCHHHHHHHHHHHHHCC
SEMTLDLRFSSPTDDHYQGMVVGFVFITFDKDHFIRGIADRLADQVLGILVIVLVVALTT
CCEEEEEEECCCCCCHHCCEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SIVFHLFISRPIIRVARGVADVDPEDPAATVLTVPRAHQRNEIGRMTNRLNHLLGRLAEV
HHHHHHHHHCHHHHHHHCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHH
QGELRLLATRDPLTDLPNRSLILEMMDATINRVALDGGRCAVMFLDLDMFKHVNDSLGHE
CCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHEEECCCCEEEEEEEHHHHHHHHHHHCCH
AGDLILKDIGRRFDAVVAGLGAVGRLGGDEFVVLIDRYEREDDLARIARRLITTVKRPIE
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCC
VDGTLVHSSTSVGIACYPVDGATPQGLLRNADTAMYAVKASGPGQWAFFDRSMTENALVR
CCCEEEECCCCCCEEEEECCCCCHHHHHCCCCCEEEEEEECCCCCEEEEECCCCCCHHEE
LRTEASLRSAIEKGQFVLHYQPKLDLRSNRISGCEALIRWDLNGDLVPPGRFISIAEETG
EEHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCEEEEEECCCCCCCCCCCEEEEEHHCC
MIYDIGLWVLREACQAQRRWRRHAGAVPIAINVSVRQLKDERFIDDFLDIIQASGADPEL
CEEEHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHHHHHHCCCCCCCE
LEVEVTESALMSSIEVSAHLLREMRSHGVRLSIDDFGTGYSSLAYLKNLPIDILKIDRSF
EEEEEHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHCCCCCEEECCHHH
VSDLPEESSIPRMIIGLAGQMGLRTVAEGIETEGQLAWLRDNGCDMIQGFLIEKPLPEAD
HHCCCCCCCCCHHHHHHCCHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHCCCCCHHH
FLRFVDAYRTARAFPSAS
HHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377; 11728710 [H]