Click here to switch to the map view.

The map label for this gene is nifE [H]

Identifier: 83593621

GI number: 83593621

Start: 2644613

End: 2646061

Strand: Reverse

Name: nifE [H]

Synonym: Rru_A2286

Alternate gene names: 83593621

Gene position: 2646061-2644613 (Counterclockwise)

Preceding gene: 83593622

Following gene: 83593620

Centisome position: 60.79

GC content: 65.63

Gene sequence:

>1449_bases
ATGATCAGCGAAAAGATCGCCAGCGTCTTCAACGAGCCCGATTGCGAGAAGAACCGCGGCAAGACGGCTGGCGAGCGCAA
AAAGGGGTGTGGCACCAAGGGGCTGACCCCCGGGGCGGCGGCCGGGGGCTGCGCCTTCGATGGTGCCAAGATCGCCCTGC
AGCCGGTGACCGATGTCGTCCATCTGGTGCACGGGCCGATCGCCTGCGAGGGTAATTCCTGGGATACGCGCAATGTTGGC
ACCTCGGGGCCGCTGATCTACCGCTGGGGCTTCACCACCGATCTGTCCGAGATGGATGTGATCCACGGCGGGGAAAAGAA
GCTGTACCGGGCGATCAAGGAGGCGGTGGCCAAATACGCGCCGCCGGCGATCTTCGTTTATCAGACCTGCGTTCCCGCCC
TGATCGGCGATGATATCGATCAGGTGTGCAAGGTCGCCTCCGAGCGTCTGGGGCTGCCGGTGGTGCCGGTCAACGCCCCG
GGCTTCGTCGGCTCGAAGAACCTGGGCAACAAACTGGGCGCCGAAGCCCTGCTCGCCCATGTCATCGGCACGATCGAGCC
TTCGGACGCCGGTCCGACCGATATCAACATCATCGGCGAATACAATGTGGCCGGCGAATTTGCCCTGGTGAAGCCGCTGT
TTGACGAATTGGGCATCCGCATCCGCGCCTGTATCACCGGCAATTCCCGCTATCGCGACTTGGCCGGCGCCCATACGGCG
CGGGTCAATATGATGGTCTGCTCGCAGGCGATGATCAACCTGGGGCGGGGCATGCGCGAGCGCTGGGGGATTCCCTACTT
CGAGGGATCGTTCTACGGCATCGGCGATACCTCGCAGACCCTGCGCACCATCGCCACGCTGTTGGTCGAACGCGGCGCCA
CGGCCGATCTGATCGCCCGCACCGAGGCCCTGATCGCCCGCGAGGAGGCCAAGGCCTGGGCGGCGATGGCCGCCTATCGG
CCGAGGCTGGCGGGCAAGCGCGTGCTGCTTTACACCGGCGGGGTCAAAAGCTGGTCGGTCGTCCACGCCTTGCAGGAAAT
GGGCATGGAGGTGGTCGGCACCTCGGTGCGCAAATCGACCGAGGACGACAAGGCCCGCGCCCGCGCCGCCCTTGATCAGA
ACACCGGCAAGGTTGGCGAGCTGTTTGGCGCCATTGCCAACCCGGAAATGTACCGGATGTTCCGCGACGGCGAGGCCGAT
ATCATGCTCTCGGGTGGGCGCTCGCAATTCGTCGCCCTGAAGGCGCGCACCGCCTGGATGGACATCAATCAGGAACGCCA
CCACGGCTATGCCGGCTATGACGGCATGCTGGAACTGGTGCGCCAGATGGATATCGAGATCAACAATCCGGTCTGGGCCC
AGGTCCGCGCCCCGGCGCCCTGGGATCCGCCGGCGGCCAGCCCCGGCGTCACGCCGCTGATCCCCGCCGGTCGGAGGGCC
GCGCCATGA

Upstream 100 bases:

>100_bases
AAACGAAAGCAAGACATTAGCTGTCTGGCATTGGTTTTGCTTACCCCTGTCCCCGAGGCCCTCTCGTCCCGTTTTCCCCG
TCCAGCCTCGGAGGATCGCC

Downstream 100 bases:

>100_bases
CCTTGTCCCCCCCCGCCACCCTGGTGCGCTCGCGCAAGGCGCTGACCGTCAGCCCGCTCAAGACCAGCGCCCCCTTGGGG
GCCGCCATGGCTTTCATGGG

Product: nitrogenase molybdenum-cofactor biosynthesis protein NifE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 482; Mature: 482

Protein sequence:

>482_residues
MISEKIASVFNEPDCEKNRGKTAGERKKGCGTKGLTPGAAAGGCAFDGAKIALQPVTDVVHLVHGPIACEGNSWDTRNVG
TSGPLIYRWGFTTDLSEMDVIHGGEKKLYRAIKEAVAKYAPPAIFVYQTCVPALIGDDIDQVCKVASERLGLPVVPVNAP
GFVGSKNLGNKLGAEALLAHVIGTIEPSDAGPTDINIIGEYNVAGEFALVKPLFDELGIRIRACITGNSRYRDLAGAHTA
RVNMMVCSQAMINLGRGMRERWGIPYFEGSFYGIGDTSQTLRTIATLLVERGATADLIARTEALIAREEAKAWAAMAAYR
PRLAGKRVLLYTGGVKSWSVVHALQEMGMEVVGTSVRKSTEDDKARARAALDQNTGKVGELFGAIANPEMYRMFRDGEAD
IMLSGGRSQFVALKARTAWMDINQERHHGYAGYDGMLELVRQMDIEINNPVWAQVRAPAPWDPPAASPGVTPLIPAGRRA
AP

Sequences:

>Translated_482_residues
MISEKIASVFNEPDCEKNRGKTAGERKKGCGTKGLTPGAAAGGCAFDGAKIALQPVTDVVHLVHGPIACEGNSWDTRNVG
TSGPLIYRWGFTTDLSEMDVIHGGEKKLYRAIKEAVAKYAPPAIFVYQTCVPALIGDDIDQVCKVASERLGLPVVPVNAP
GFVGSKNLGNKLGAEALLAHVIGTIEPSDAGPTDINIIGEYNVAGEFALVKPLFDELGIRIRACITGNSRYRDLAGAHTA
RVNMMVCSQAMINLGRGMRERWGIPYFEGSFYGIGDTSQTLRTIATLLVERGATADLIARTEALIAREEAKAWAAMAAYR
PRLAGKRVLLYTGGVKSWSVVHALQEMGMEVVGTSVRKSTEDDKARARAALDQNTGKVGELFGAIANPEMYRMFRDGEAD
IMLSGGRSQFVALKARTAWMDINQERHHGYAGYDGMLELVRQMDIEINNPVWAQVRAPAPWDPPAASPGVTPLIPAGRRA
AP
>Mature_482_residues
MISEKIASVFNEPDCEKNRGKTAGERKKGCGTKGLTPGAAAGGCAFDGAKIALQPVTDVVHLVHGPIACEGNSWDTRNVG
TSGPLIYRWGFTTDLSEMDVIHGGEKKLYRAIKEAVAKYAPPAIFVYQTCVPALIGDDIDQVCKVASERLGLPVVPVNAP
GFVGSKNLGNKLGAEALLAHVIGTIEPSDAGPTDINIIGEYNVAGEFALVKPLFDELGIRIRACITGNSRYRDLAGAHTA
RVNMMVCSQAMINLGRGMRERWGIPYFEGSFYGIGDTSQTLRTIATLLVERGATADLIARTEALIAREEAKAWAAMAAYR
PRLAGKRVLLYTGGVKSWSVVHALQEMGMEVVGTSVRKSTEDDKARARAALDQNTGKVGELFGAIANPEMYRMFRDGEAD
IMLSGGRSQFVALKARTAWMDINQERHHGYAGYDGMLELVRQMDIEINNPVWAQVRAPAPWDPPAASPGVTPLIPAGRRA
AP

Specific function: This protein may play a role in the biosynthesis of the prosthetic group of nitrogenase (FeMo cofactor) [H]

COG id: COG2710

COG function: function code C; Nitrogenase molybdenum-iron protein, alpha and beta chains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nifD/nifK/nifE/nifN family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000510
- InterPro:   IPR000318
- InterPro:   IPR005973 [H]

Pfam domain/function: PF00148 Oxidored_nitro [H]

EC number: NA

Molecular weight: Translated: 51837; Mature: 51837

Theoretical pI: Translated: 8.00; Mature: 8.00

Prosite motif: PS00699 NITROGENASE_1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISEKIASVFNEPDCEKNRGKTAGERKKGCGTKGLTPGAAAGGCAFDGAKIALQPVTDVV
CCHHHHHHHHCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCEEEEHHHHHHH
HLVHGPIACEGNSWDTRNVGTSGPLIYRWGFTTDLSEMDVIHGGEKKLYRAIKEAVAKYA
HHHCCCEEECCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHCCCHHHHHHHHHHHHHHHC
PPAIFVYQTCVPALIGDDIDQVCKVASERLGLPVVPVNAPGFVGSKNLGNKLGAEALLAH
CCEEEEHHHHHHHHHCCCHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCHHHHHHHHHHH
VIGTIEPSDAGPTDINIIGEYNVAGEFALVKPLFDELGIRIRACITGNSRYRDLAGAHTA
HHHCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHCCCEEEEEEECCCCHHHHHCCHHH
RVNMMVCSQAMINLGRGMRERWGIPYFEGSFYGIGDTSQTLRTIATLLVERGATADLIAR
HHHHHHHHHHHHHHHCCHHHHCCCCCCCCCEEECCCHHHHHHHHHHHHHHCCCCHHHHHH
TEALIAREEAKAWAAMAAYRPRLAGKRVLLYTGGVKSWSVVHALQEMGMEVVGTSVRKST
HHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHCCHHHHHHHHHCCC
EDDKARARAALDQNTGKVGELFGAIANPEMYRMFRDGEADIMLSGGRSQFVALKARTAWM
CHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHCCCCCEEEEECCCCEEEEEEEEEHEE
DINQERHHGYAGYDGMLELVRQMDIEINNPVWAQVRAPAPWDPPAASPGVTPLIPAGRRA
CCCHHHCCCCCCHHHHHHHHHHHCCEECCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCC
AP
CC
>Mature Secondary Structure
MISEKIASVFNEPDCEKNRGKTAGERKKGCGTKGLTPGAAAGGCAFDGAKIALQPVTDVV
CCHHHHHHHHCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCEEEEHHHHHHH
HLVHGPIACEGNSWDTRNVGTSGPLIYRWGFTTDLSEMDVIHGGEKKLYRAIKEAVAKYA
HHHCCCEEECCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHCCCHHHHHHHHHHHHHHHC
PPAIFVYQTCVPALIGDDIDQVCKVASERLGLPVVPVNAPGFVGSKNLGNKLGAEALLAH
CCEEEEHHHHHHHHHCCCHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCHHHHHHHHHHH
VIGTIEPSDAGPTDINIIGEYNVAGEFALVKPLFDELGIRIRACITGNSRYRDLAGAHTA
HHHCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHCCCEEEEEEECCCCHHHHHCCHHH
RVNMMVCSQAMINLGRGMRERWGIPYFEGSFYGIGDTSQTLRTIATLLVERGATADLIAR
HHHHHHHHHHHHHHHCCHHHHCCCCCCCCCEEECCCHHHHHHHHHHHHHHCCCCHHHHHH
TEALIAREEAKAWAAMAAYRPRLAGKRVLLYTGGVKSWSVVHALQEMGMEVVGTSVRKST
HHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHCCHHHHHHHHHCCC
EDDKARARAALDQNTGKVGELFGAIANPEMYRMFRDGEADIMLSGGRSQFVALKARTAWM
CHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHCCCCCEEEEECCCCEEEEEEEEEHEE
DINQERHHGYAGYDGMLELVRQMDIEINNPVWAQVRAPAPWDPPAASPGVTPLIPAGRRA
CCCHHHCCCCCCHHHHHHHHHHHCCEECCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCC
AP
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11214968 [H]