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The map label for this gene is fixA [H]

Identifier: 83593602

GI number: 83593602

Start: 2627664

End: 2628515

Strand: Reverse

Name: fixA [H]

Synonym: Rru_A2267

Alternate gene names: 83593602

Gene position: 2628515-2627664 (Counterclockwise)

Preceding gene: 83593603

Following gene: 83593601

Centisome position: 60.39

GC content: 64.08

Gene sequence:

>852_bases
ATGCATATCGTTGTCTGCATCAAGCAGGTTCCCGACAGCGCGCAAATCCGGGTGCATCCGGTGACCAACACCATCATGCG
CCAGGGCGTGCCGACCATCATCAACCCCTATGATCTTTTCGCCCTTGAAGAGGCCCTGCGGCTTCGTGACAAAATGGGTG
GCAAGGTGACCGTTCTGACCATGGGTCCGCCCATGGCCGATGAAAGCTTGCGCAAGGCCTTAGGGCTAGGCGCCGATGCC
GCCGTGCTGTTGACCGACCGTTTCTTCGCCGGGTCCGATACCCTGGCCACCTCCTATGCCCTGGCCGCCGGGATCCGCAA
GATCGGCGAGGAGGAGCCCGTCGATATCGTCTTCACCGGCAAGCAGACGATCGACGGCGATACCGCCCAGGTCGGGCCGG
GGATCGCCAAGCGGCTGGGTCTTAACCAGTTGACCTATGTGGCGGCCATCCGCTCGCTCGATCTGGAAAACCGCTCGATC
GTCGTCGAACGCCGGGCCGAGGGCGGCGTGCAGGTGCTAAGCACCGGGCTGCCTTGCCTGATCACCATGCTTGAAGGCAC
GAACACCCTGCGCCGCGGCGCCATGGACGACATGTTCCGCGCCTCGCGCGCCACCATCGCCACCTGGTCGGCCCAACAGG
CCGGCGTCGATGATATCGCGCTGTGCGGTTTGCGTGGCTCGCCGACGGTGGTGAAGAAGGTCTTCGCGCCGCAACCGCGC
AAGGAAAAGGCCAAGATGGTCGAGACCGCCGGCAAGTCCGATGCCGAGATCGCCCAGGCCACCCTGGATACCCTGTTTTC
CGCCAATCCGAAGCTGGAGACCGACCTCCGTAAGCTGGCCGCGGCGTCGTAA

Upstream 100 bases:

>100_bases
GTCCCTCGAAAGATTAGGACGCCAAAAACGGAAGTTCGGCGGGATAACCCGCAGAGGACTTTTCCGCTCCCCCACCGGGA
GCCCCGCGACGGAGGAAACC

Downstream 100 bases:

>100_bases
TACGGAGGAGCGAGAGATGAGCGAAGAGAAGAAGCCCGCCCCGGCGGCGGCCGGCCGTGGCGCCGCCAAAAAAGAGCTGT
CCGAGCATTTCAAGACCTAT

Product: electron transfer flavoprotein subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 283; Mature: 283

Protein sequence:

>283_residues
MHIVVCIKQVPDSAQIRVHPVTNTIMRQGVPTIINPYDLFALEEALRLRDKMGGKVTVLTMGPPMADESLRKALGLGADA
AVLLTDRFFAGSDTLATSYALAAGIRKIGEEEPVDIVFTGKQTIDGDTAQVGPGIAKRLGLNQLTYVAAIRSLDLENRSI
VVERRAEGGVQVLSTGLPCLITMLEGTNTLRRGAMDDMFRASRATIATWSAQQAGVDDIALCGLRGSPTVVKKVFAPQPR
KEKAKMVETAGKSDAEIAQATLDTLFSANPKLETDLRKLAAAS

Sequences:

>Translated_283_residues
MHIVVCIKQVPDSAQIRVHPVTNTIMRQGVPTIINPYDLFALEEALRLRDKMGGKVTVLTMGPPMADESLRKALGLGADA
AVLLTDRFFAGSDTLATSYALAAGIRKIGEEEPVDIVFTGKQTIDGDTAQVGPGIAKRLGLNQLTYVAAIRSLDLENRSI
VVERRAEGGVQVLSTGLPCLITMLEGTNTLRRGAMDDMFRASRATIATWSAQQAGVDDIALCGLRGSPTVVKKVFAPQPR
KEKAKMVETAGKSDAEIAQATLDTLFSANPKLETDLRKLAAAS
>Mature_283_residues
MHIVVCIKQVPDSAQIRVHPVTNTIMRQGVPTIINPYDLFALEEALRLRDKMGGKVTVLTMGPPMADESLRKALGLGADA
AVLLTDRFFAGSDTLATSYALAAGIRKIGEEEPVDIVFTGKQTIDGDTAQVGPGIAKRLGLNQLTYVAAIRSLDLENRSI
VVERRAEGGVQVLSTGLPCLITMLEGTNTLRRGAMDDMFRASRATIATWSAQQAGVDDIALCGLRGSPTVVKKVFAPQPR
KEKAKMVETAGKSDAEIAQATLDTLFSANPKLETDLRKLAAAS

Specific function: May play a role in a redox process involved in nitrogen fixation [H]

COG id: COG2086

COG function: function code C; Electron transfer flavoprotein, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ETF beta-subunit/fixA family [H]

Homologues:

Organism=Homo sapiens, GI4503609, Length=219, Percent_Identity=31.5068493150685, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI62420877, Length=206, Percent_Identity=31.5533980582524, Blast_Score=90, Evalue=3e-18,
Organism=Escherichia coli, GI2367123, Length=239, Percent_Identity=27.6150627615063, Blast_Score=67, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI25141345, Length=221, Percent_Identity=32.579185520362, Blast_Score=100, Evalue=8e-22,
Organism=Saccharomyces cerevisiae, GI6321646, Length=210, Percent_Identity=30, Blast_Score=75, Evalue=8e-15,
Organism=Drosophila melanogaster, GI24651147, Length=217, Percent_Identity=29.0322580645161, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24651145, Length=217, Percent_Identity=29.0322580645161, Blast_Score=87, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000049
- InterPro:   IPR014730
- InterPro:   IPR012255
- InterPro:   IPR014729 [H]

Pfam domain/function: PF01012 ETF [H]

EC number: NA

Molecular weight: Translated: 30258; Mature: 30258

Theoretical pI: Translated: 8.47; Mature: 8.47

Prosite motif: PS01065 ETF_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHIVVCIKQVPDSAQIRVHPVTNTIMRQGVPTIINPYDLFALEEALRLRDKMGGKVTVLT
CEEEEEEEECCCCCCEEEEECHHHHHHCCCCCEECCHHHHHHHHHHHHHHHCCCEEEEEE
MGPPMADESLRKALGLGADAAVLLTDRFFAGSDTLATSYALAAGIRKIGEEEPVDIVFTG
CCCCCCHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEC
KQTIDGDTAQVGPGIAKRLGLNQLTYVAAIRSLDLENRSIVVERRAEGGVQVLSTGLPCL
CCCCCCCCCCCCCHHHHHHCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCEEHHCCHHEE
ITMLEGTNTLRRGAMDDMFRASRATIATWSAQQAGVDDIALCGLRGSPTVVKKVFAPQPR
HHHHCCCHHHHHCCHHHHHHHHCCEEEECCCCCCCCCCEEEECCCCCHHHHHHHHCCCCH
KEKAKMVETAGKSDAEIAQATLDTLFSANPKLETDLRKLAAAS
HHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCC
>Mature Secondary Structure
MHIVVCIKQVPDSAQIRVHPVTNTIMRQGVPTIINPYDLFALEEALRLRDKMGGKVTVLT
CEEEEEEEECCCCCCEEEEECHHHHHHCCCCCEECCHHHHHHHHHHHHHHHCCCEEEEEE
MGPPMADESLRKALGLGADAAVLLTDRFFAGSDTLATSYALAAGIRKIGEEEPVDIVFTG
CCCCCCHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEC
KQTIDGDTAQVGPGIAKRLGLNQLTYVAAIRSLDLENRSIVVERRAEGGVQVLSTGLPCL
CCCCCCCCCCCCCHHHHHHCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCEEHHCCHHEE
ITMLEGTNTLRRGAMDDMFRASRATIATWSAQQAGVDDIALCGLRGSPTVVKKVFAPQPR
HHHHCCCHHHHHCCHHHHHHHHCCEEEECCCCCCCCCCEEEECCCCCHHHHHHHHCCCCH
KEKAKMVETAGKSDAEIAQATLDTLFSANPKLETDLRKLAAAS
HHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8796346; 9163424 [H]