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The map label for this gene is gcvA [H]

Identifier: 83593550

GI number: 83593550

Start: 2574265

End: 2575218

Strand: Reverse

Name: gcvA [H]

Synonym: Rru_A2215

Alternate gene names: 83593550

Gene position: 2575218-2574265 (Counterclockwise)

Preceding gene: 83593558

Following gene: 83593549

Centisome position: 59.16

GC content: 70.02

Gene sequence:

>954_bases
GTGGTCCGCCGCCTGCCGCCGCTCAACGCGTTGCGCGCCTTCGAGGCCGCCGCCCGCCACGGCGGGTTTACCGGCGCCGC
CCGCGAGTTGCATGTCACTCCGGCGGCGGTCAGCCATCAGGTGAAGGGGTTGGAGGACGCCTTGGGCGTCAGCCTCTTTC
ATCGCCTTGCCAAGGGGTTGGAGCTGACGGCGGCCGGGCGGGCCTATCTGCCCGAACTCACCGCCGGCTTTGATCAATTG
GCCCGCGCCAGCGAGCGGCTCAAGCGCGGCGCCCTGGTCGGCCGGCTGACGGTCAGCGTCCTGCCGTCCTTCGCCGCCGG
CTGGCTGGCGCCGCGGCTGGCTTCGTTCCTGCGCCGCTATCCCGATATCCTGCTTGATCTGCGCTCGACGCCGCGCACGG
TCGACTTTATCCGCGAACGCGTTGATCTGGGGATCCGCTATGGCGGCGGCATCTATCCGGGTCTGGCGAGCTACCGCATC
CTCGAGGAAGAGGTTTTCCCGATCTGCGCCCCGGCCCTGCTCAACGGTCCGGTGCCGCTGCGCGGCTTCGCCGATCTGCG
CCATCACGTTTTGCTCCACGACCCGGGGATCCTGCCCGGCGAGCCGTGGACGGGGTGGGAGCCCTGGCTGGCCGAGGCCG
GGGTGACGGATATCGATCTCGAGGCCGGTCACGGCTTTACCGAATCGATCGCCATGATCCATGCCTGCGTCGCCGGCCTG
GGGGTTGGCATCGGACGGTCGGGACTGGTCACCGAACTCCTGCGCTCGGGACGGCTGGTCCGCCCCTTTCCGGTGTCCCG
CCCAGCCGACTTCAGTTACTGGGTGGTGGTGCCGCCGCCGTCGCAGACCAACCCCAAGGCGGCGGCCTTCATCGGCTGGC
TGCTGGAAACCGCCGGTCGCGGTGGCGCCGGCGCCGGGGCGCAGCCCGATCTTCTTGGCGAGGATCCGTCATGA

Upstream 100 bases:

>100_bases
CGGCGTCTGTTAAGAAGATGAGGATCTTGACGAAGGGCCGCAAACGAGGCTTTCTCGGCACGAGGTTTAGGAAAAATAAG
CCATCCGCAAAGGGACTGCC

Downstream 100 bases:

>100_bases
GCCGCCGTCTGCCGCCGCTTAACGCCCTGCGCGCCTTCGAGGCCGCCGCCCGCCATGGCGGGTTCACCCGGGCGGCCGAG
GAACTGGGGGTGACTCCGGC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 317; Mature: 317

Protein sequence:

>317_residues
MVRRLPPLNALRAFEAAARHGGFTGAARELHVTPAAVSHQVKGLEDALGVSLFHRLAKGLELTAAGRAYLPELTAGFDQL
ARASERLKRGALVGRLTVSVLPSFAAGWLAPRLASFLRRYPDILLDLRSTPRTVDFIRERVDLGIRYGGGIYPGLASYRI
LEEEVFPICAPALLNGPVPLRGFADLRHHVLLHDPGILPGEPWTGWEPWLAEAGVTDIDLEAGHGFTESIAMIHACVAGL
GVGIGRSGLVTELLRSGRLVRPFPVSRPADFSYWVVVPPPSQTNPKAAAFIGWLLETAGRGGAGAGAQPDLLGEDPS

Sequences:

>Translated_317_residues
MVRRLPPLNALRAFEAAARHGGFTGAARELHVTPAAVSHQVKGLEDALGVSLFHRLAKGLELTAAGRAYLPELTAGFDQL
ARASERLKRGALVGRLTVSVLPSFAAGWLAPRLASFLRRYPDILLDLRSTPRTVDFIRERVDLGIRYGGGIYPGLASYRI
LEEEVFPICAPALLNGPVPLRGFADLRHHVLLHDPGILPGEPWTGWEPWLAEAGVTDIDLEAGHGFTESIAMIHACVAGL
GVGIGRSGLVTELLRSGRLVRPFPVSRPADFSYWVVVPPPSQTNPKAAAFIGWLLETAGRGGAGAGAQPDLLGEDPS
>Mature_317_residues
MVRRLPPLNALRAFEAAARHGGFTGAARELHVTPAAVSHQVKGLEDALGVSLFHRLAKGLELTAAGRAYLPELTAGFDQL
ARASERLKRGALVGRLTVSVLPSFAAGWLAPRLASFLRRYPDILLDLRSTPRTVDFIRERVDLGIRYGGGIYPGLASYRI
LEEEVFPICAPALLNGPVPLRGFADLRHHVLLHDPGILPGEPWTGWEPWLAEAGVTDIDLEAGHGFTESIAMIHACVAGL
GVGIGRSGLVTELLRSGRLVRPFPVSRPADFSYWVVVPPPSQTNPKAAAFIGWLLETAGRGGAGAGAQPDLLGEDPS

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=299, Percent_Identity=40.133779264214, Blast_Score=219, Evalue=2e-58,
Organism=Escherichia coli, GI1788706, Length=290, Percent_Identity=33.7931034482759, Blast_Score=161, Evalue=4e-41,
Organism=Escherichia coli, GI1786448, Length=306, Percent_Identity=33.0065359477124, Blast_Score=128, Evalue=5e-31,
Organism=Escherichia coli, GI157672245, Length=167, Percent_Identity=36.5269461077844, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1786401, Length=304, Percent_Identity=25.3289473684211, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI145693193, Length=300, Percent_Identity=25.3333333333333, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1787128, Length=265, Percent_Identity=26.7924528301887, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI87081978, Length=303, Percent_Identity=27.0627062706271, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1788887, Length=160, Percent_Identity=31.25, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI1787601, Length=172, Percent_Identity=27.906976744186, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1789440, Length=302, Percent_Identity=22.5165562913907, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1789639, Length=155, Percent_Identity=29.0322580645161, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1788748, Length=168, Percent_Identity=30.952380952381, Blast_Score=61, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33909; Mature: 33909

Theoretical pI: Translated: 8.27; Mature: 8.27

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVRRLPPLNALRAFEAAARHGGFTGAARELHVTPAAVSHQVKGLEDALGVSLFHRLAKGL
CCCCCCCHHHHHHHHHHHHCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCC
ELTAAGRAYLPELTAGFDQLARASERLKRGALVGRLTVSVLPSFAAGWLAPRLASFLRRY
EEEECCCHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PDILLDLRSTPRTVDFIRERVDLGIRYGGGIYPGLASYRILEEEVFPICAPALLNGPVPL
HHHHHHHCCCCHHHHHHHHHHHCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
RGFADLRHHVLLHDPGILPGEPWTGWEPWLAEAGVTDIDLEAGHGFTESIAMIHACVAGL
CHHHHHHHEEEEECCCCCCCCCCCCCCHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHH
GVGIGRSGLVTELLRSGRLVRPFPVSRPADFSYWVVVPPPSQTNPKAAAFIGWLLETAGR
HCCCCCHHHHHHHHHCCCEECCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCC
GGAGAGAQPDLLGEDPS
CCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MVRRLPPLNALRAFEAAARHGGFTGAARELHVTPAAVSHQVKGLEDALGVSLFHRLAKGL
CCCCCCCHHHHHHHHHHHHCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCC
ELTAAGRAYLPELTAGFDQLARASERLKRGALVGRLTVSVLPSFAAGWLAPRLASFLRRY
EEEECCCHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PDILLDLRSTPRTVDFIRERVDLGIRYGGGIYPGLASYRILEEEVFPICAPALLNGPVPL
HHHHHHHCCCCHHHHHHHHHHHCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
RGFADLRHHVLLHDPGILPGEPWTGWEPWLAEAGVTDIDLEAGHGFTESIAMIHACVAGL
CHHHHHHHEEEEECCCCCCCCCCCCCCHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHH
GVGIGRSGLVTELLRSGRLVRPFPVSRPADFSYWVVVPPPSQTNPKAAAFIGWLLETAGR
HCCCCCHHHHHHHHHCCCEECCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCC
GGAGAGAQPDLLGEDPS
CCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]