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The map label for this gene is gcvA [H]

Identifier: 83593549

GI number: 83593549

Start: 2573342

End: 2574268

Strand: Reverse

Name: gcvA [H]

Synonym: Rru_A2214

Alternate gene names: 83593549

Gene position: 2574268-2573342 (Counterclockwise)

Preceding gene: 83593550

Following gene: 83593548

Centisome position: 59.14

GC content: 70.33

Gene sequence:

>927_bases
ATGAGCCGCCGTCTGCCGCCGCTTAACGCCCTGCGCGCCTTCGAGGCCGCCGCCCGCCATGGCGGGTTCACCCGGGCGGC
CGAGGAACTGGGGGTGACTCCGGCCGCCGTCAGTCATCAGGTCAAGGGCCTGGAAGACGTGCTGGGCCTGTGCCTGTTCC
GCCGCCTGCCGCGCGGTCTGGAATTGACCGAGGAGGGGCAGGCCTATCTGCCAGACCTCAGCCGGGGCTTTGATCGCCTG
GCCCGCGCCGGCGAACGCCTGCGCGAGGGCGGACTGGCCGGCCGGTTGACGGTGTCGGTGCTGCCGAGCTTTTGCGCCAA
TTGGCTGGCGCCGCGCCTGCCCGACTTCCAGGCGGCCTGCCCGGAGGTTCATCTGCGCATGCTGTCGGAAAACCGCTTCG
CCGACTTCAACCGCGATGGCATCGACATGGCCATCCGCTATGGCCGGGGCATCTATCCCGGCCTGCGCGCCATTCCGTTT
CTGGCCGAGGAGGTCTTCCCGGTCTGCGCGCCCTCGTTGCTCAATGGGGCCTTGCCGCTGCGCGGCTTGGCCGATCTGCG
CCACCACACGCTTCTGCGCAGCATCGACGAGACCGCCGGCGAGCCCTGGCTTTATTGGCGGCTGTGGTTGGCCGCCGAAG
GCATTGAAGGGGTAGCCCCCGATGGCGGGATCGGCTTCACCGATGGCTCGGCCCTGGTCCAGGCCTGTCTGGCCGGCGTC
GGTGTCGCCCTGGGACGCACCGCCCTGGTCGAGGACCACCTGCGCACCGGGCGTTTGGTCCGGCCCTTCGCCGGTCTCAG
CCGCCCGGCCGATTTCTCCTATTTCGCCGTCGCCCCGGCGGCCAATTTGGAAAACCCCCGGGTCGCCTGCTTCATCGACT
GGCTGCGCGCCGCCGGCGTGCCCGCTTGCCTAGCCGACGGGGGGTGA

Upstream 100 bases:

>100_bases
ACCCCAAGGCGGCGGCCTTCATCGGCTGGCTGCTGGAAACCGCCGGTCGCGGTGGCGCCGGCGCCGGGGCGCAGCCCGAT
CTTCTTGGCGAGGATCCGTC

Downstream 100 bases:

>100_bases
CGCTTCCCCGTTCCTCCCGTTCCCTCACGCCGCTCCGCTGCCGCTTCTCCTTGATAAGGACTCACCGCGATGCCCCGCCT
GCCCAGACCGCCCGGCTCTT

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 308; Mature: 307

Protein sequence:

>308_residues
MSRRLPPLNALRAFEAAARHGGFTRAAEELGVTPAAVSHQVKGLEDVLGLCLFRRLPRGLELTEEGQAYLPDLSRGFDRL
ARAGERLREGGLAGRLTVSVLPSFCANWLAPRLPDFQAACPEVHLRMLSENRFADFNRDGIDMAIRYGRGIYPGLRAIPF
LAEEVFPVCAPSLLNGALPLRGLADLRHHTLLRSIDETAGEPWLYWRLWLAAEGIEGVAPDGGIGFTDGSALVQACLAGV
GVALGRTALVEDHLRTGRLVRPFAGLSRPADFSYFAVAPAANLENPRVACFIDWLRAAGVPACLADGG

Sequences:

>Translated_308_residues
MSRRLPPLNALRAFEAAARHGGFTRAAEELGVTPAAVSHQVKGLEDVLGLCLFRRLPRGLELTEEGQAYLPDLSRGFDRL
ARAGERLREGGLAGRLTVSVLPSFCANWLAPRLPDFQAACPEVHLRMLSENRFADFNRDGIDMAIRYGRGIYPGLRAIPF
LAEEVFPVCAPSLLNGALPLRGLADLRHHTLLRSIDETAGEPWLYWRLWLAAEGIEGVAPDGGIGFTDGSALVQACLAGV
GVALGRTALVEDHLRTGRLVRPFAGLSRPADFSYFAVAPAANLENPRVACFIDWLRAAGVPACLADGG
>Mature_307_residues
SRRLPPLNALRAFEAAARHGGFTRAAEELGVTPAAVSHQVKGLEDVLGLCLFRRLPRGLELTEEGQAYLPDLSRGFDRLA
RAGERLREGGLAGRLTVSVLPSFCANWLAPRLPDFQAACPEVHLRMLSENRFADFNRDGIDMAIRYGRGIYPGLRAIPFL
AEEVFPVCAPSLLNGALPLRGLADLRHHTLLRSIDETAGEPWLYWRLWLAAEGIEGVAPDGGIGFTDGSALVQACLAGVG
VALGRTALVEDHLRTGRLVRPFAGLSRPADFSYFAVAPAANLENPRVACFIDWLRAAGVPACLADGG

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=299, Percent_Identity=43.4782608695652, Blast_Score=234, Evalue=8e-63,
Organism=Escherichia coli, GI1788706, Length=293, Percent_Identity=33.1058020477816, Blast_Score=156, Evalue=1e-39,
Organism=Escherichia coli, GI1786448, Length=292, Percent_Identity=33.2191780821918, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI1786401, Length=287, Percent_Identity=27.8745644599303, Blast_Score=101, Evalue=5e-23,
Organism=Escherichia coli, GI87081978, Length=299, Percent_Identity=29.4314381270903, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI145693193, Length=298, Percent_Identity=27.1812080536913, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI157672245, Length=162, Percent_Identity=33.9506172839506, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1787128, Length=250, Percent_Identity=25.6, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1789440, Length=280, Percent_Identity=25.3571428571429, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33228; Mature: 33096

Theoretical pI: Translated: 7.08; Mature: 7.08

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRRLPPLNALRAFEAAARHGGFTRAAEELGVTPAAVSHQVKGLEDVLGLCLFRRLPRGL
CCCCCCCHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHCCCCC
ELTEEGQAYLPDLSRGFDRLARAGERLREGGLAGRLTVSVLPSFCANWLAPRLPDFQAAC
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHH
PEVHLRMLSENRFADFNRDGIDMAIRYGRGIYPGLRAIPFLAEEVFPVCAPSLLNGALPL
HHHHHHHHHCCCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCH
RGLADLRHHTLLRSIDETAGEPWLYWRLWLAAEGIEGVAPDGGIGFTDGSALVQACLAGV
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHH
GVALGRTALVEDHLRTGRLVRPFAGLSRPADFSYFAVAPAANLENPRVACFIDWLRAAGV
HHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHCCC
PACLADGG
CCCCCCCC
>Mature Secondary Structure 
SRRLPPLNALRAFEAAARHGGFTRAAEELGVTPAAVSHQVKGLEDVLGLCLFRRLPRGL
CCCCCCHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHCCCCC
ELTEEGQAYLPDLSRGFDRLARAGERLREGGLAGRLTVSVLPSFCANWLAPRLPDFQAAC
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHH
PEVHLRMLSENRFADFNRDGIDMAIRYGRGIYPGLRAIPFLAEEVFPVCAPSLLNGALPL
HHHHHHHHHCCCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCH
RGLADLRHHTLLRSIDETAGEPWLYWRLWLAAEGIEGVAPDGGIGFTDGSALVQACLAGV
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHH
GVALGRTALVEDHLRTGRLVRPFAGLSRPADFSYFAVAPAANLENPRVACFIDWLRAAGV
HHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHCCC
PACLADGG
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]