Click here to switch to the map view.

The map label for this gene is purN [H]

Identifier: 83593503

GI number: 83593503

Start: 2517422

End: 2518096

Strand: Direct

Name: purN [H]

Synonym: Rru_A2168

Alternate gene names: 83593503

Gene position: 2517422-2518096 (Clockwise)

Preceding gene: 83593502

Following gene: 83593505

Centisome position: 57.83

GC content: 67.41

Gene sequence:

>675_bases
ATGAACGCTCCCCAATCGACCGCGCCCCGCAAGCGCGTGGCGGTACTGATCAGCGGGCGCGGCAGCAATATGGAAGCGCT
GATCGCGGCTTGCGCCGATCCCGCCTTCCCCGCCGGCATCGTCAGCGTGATTTCCAACCGCGCCGACGCCAAGGGTCTGG
AGCGGGCGCAAGCCGCCGGCTTGTCGACCACGGTGATCGACCACAAGGCCTTCGCCGGGCGTGAACCCTTCGAAGCCGCG
CTCAGCGCCCATATCGAAGCCGTCGGCGCCGATATCATCTGTCTGGCGGGCTTCATGCGCCTGCTGACGGCGGGGTTCGT
CACCCGCTGGCAAGACAGGATGATCAACATCCATCCGTCGCTGATCCCGGCCTTCCGCGGCCTGCATACCCATGAACGGG
TGATCGAGGCCGGGGTGCGCGTTCATGGCTGCACGGTTCACTTCGTGCGCGCCGAAATGGACGACGGCCCGATCATCGTC
CAGGCCGCCCTGCCCGTCCGCCCCGATGATACCGCCGACAGCCTGGGGGCACGGGTGCTGACCCGGGAACATCAGATCTA
CCCGCTGGCCCTGCGCCTGCTGGCCGAGGGCAAGGTGCGCGTTGAAGGCAATCGCGCCATCATCGACGCCGCCGCCGACG
ATATCACCCTGATCAACCCGCCGCTCAGCGCCTGA

Upstream 100 bases:

>100_bases
TTGGCGAACACGGTGAAACCGTTCATCGCCTGGGCACCATCGCCGCGCGCGGCGAGGGCGAGGCGGTGATCATCGATCAC
CTGGACGAAGCCTTCGCCCG

Downstream 100 bases:

>100_bases
CGGCCGGCGGCCCAAAACAAAACCCGCCCCTTTCGGGGCGGGTTTCGCAGTGACACAAATCGTCGTCGCACCCTGGTCGA
GAGTGCTCGGGGATTTGCTT

Product: phosphoribosylglycinamide formyltransferase

Products: NA

Alternate protein names: 5'-phosphoribosylglycinamide transformylase; GAR transformylase; GART [H]

Number of amino acids: Translated: 224; Mature: 224

Protein sequence:

>224_residues
MNAPQSTAPRKRVAVLISGRGSNMEALIAACADPAFPAGIVSVISNRADAKGLERAQAAGLSTTVIDHKAFAGREPFEAA
LSAHIEAVGADIICLAGFMRLLTAGFVTRWQDRMINIHPSLIPAFRGLHTHERVIEAGVRVHGCTVHFVRAEMDDGPIIV
QAALPVRPDDTADSLGARVLTREHQIYPLALRLLAEGKVRVEGNRAIIDAAADDITLINPPLSA

Sequences:

>Translated_224_residues
MNAPQSTAPRKRVAVLISGRGSNMEALIAACADPAFPAGIVSVISNRADAKGLERAQAAGLSTTVIDHKAFAGREPFEAA
LSAHIEAVGADIICLAGFMRLLTAGFVTRWQDRMINIHPSLIPAFRGLHTHERVIEAGVRVHGCTVHFVRAEMDDGPIIV
QAALPVRPDDTADSLGARVLTREHQIYPLALRLLAEGKVRVEGNRAIIDAAADDITLINPPLSA
>Mature_224_residues
MNAPQSTAPRKRVAVLISGRGSNMEALIAACADPAFPAGIVSVISNRADAKGLERAQAAGLSTTVIDHKAFAGREPFEAA
LSAHIEAVGADIICLAGFMRLLTAGFVTRWQDRMINIHPSLIPAFRGLHTHERVIEAGVRVHGCTVHFVRAEMDDGPIIV
QAALPVRPDDTADSLGARVLTREHQIYPLALRLLAEGKVRVEGNRAIIDAAADDITLINPPLSA

Specific function: De novo purine biosynthesis; third step. [C]

COG id: COG0299

COG function: function code F; Folate-dependent phosphoribosylglycinamide formyltransferase PurN

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GART family [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=198, Percent_Identity=48.989898989899, Blast_Score=199, Evalue=2e-51,
Organism=Homo sapiens, GI209869995, Length=198, Percent_Identity=48.989898989899, Blast_Score=199, Evalue=2e-51,
Organism=Homo sapiens, GI209869993, Length=198, Percent_Identity=48.989898989899, Blast_Score=199, Evalue=2e-51,
Organism=Escherichia coli, GI1788846, Length=199, Percent_Identity=44.7236180904523, Blast_Score=188, Evalue=2e-49,
Organism=Escherichia coli, GI1787483, Length=201, Percent_Identity=29.3532338308458, Blast_Score=94, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17567511, Length=186, Percent_Identity=38.7096774193548, Blast_Score=142, Evalue=1e-34,
Organism=Saccharomyces cerevisiae, GI6320616, Length=199, Percent_Identity=32.6633165829146, Blast_Score=76, Evalue=5e-15,
Organism=Drosophila melanogaster, GI24582400, Length=190, Percent_Identity=52.6315789473684, Blast_Score=197, Evalue=6e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR001555
- InterPro:   IPR004607 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =2.1.2.2 [H]

Molecular weight: Translated: 23855; Mature: 23855

Theoretical pI: Translated: 7.25; Mature: 7.25

Prosite motif: PS00373 GART

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAPQSTAPRKRVAVLISGRGSNMEALIAACADPAFPAGIVSVISNRADAKGLERAQAAG
CCCCCCCCCCEEEEEEEECCCCCHHHHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHHHCC
LSTTVIDHKAFAGREPFEAALSAHIEAVGADIICLAGFMRLLTAGFVTRWQDRMINIHPS
CEEEEECCHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHEEEECHH
LIPAFRGLHTHERVIEAGVRVHGCTVHFVRAEMDDGPIIVQAALPVRPDDTADSLGARVL
HHHHHHCCCHHHHHHHCCCEEEEEEEEEEEEECCCCCEEEEEECCCCCCCCHHHHHHHHH
TREHQIYPLALRLLAEGKVRVEGNRAIIDAAADDITLINPPLSA
HCCCCHHHHHHHHHHCCEEEECCCEEEEEECCCCEEEECCCCCC
>Mature Secondary Structure
MNAPQSTAPRKRVAVLISGRGSNMEALIAACADPAFPAGIVSVISNRADAKGLERAQAAG
CCCCCCCCCCEEEEEEEECCCCCHHHHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHHHCC
LSTTVIDHKAFAGREPFEAALSAHIEAVGADIICLAGFMRLLTAGFVTRWQDRMINIHPS
CEEEEECCHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHEEEECHH
LIPAFRGLHTHERVIEAGVRVHGCTVHFVRAEMDDGPIIVQAALPVRPDDTADSLGARVL
HHHHHHCCCHHHHHHHCCCEEEEEEEEEEEEECCCCCEEEEEECCCCCCCCHHHHHHHHH
TREHQIYPLALRLLAEGKVRVEGNRAIIDAAADDITLINPPLSA
HCCCCHHHHHHHHHHCCEEEECCCEEEEEECCCCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]