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The map label for this gene is luxO [H]

Identifier: 83593493

GI number: 83593493

Start: 2505430

End: 2506965

Strand: Reverse

Name: luxO [H]

Synonym: Rru_A2158

Alternate gene names: 83593493

Gene position: 2506965-2505430 (Counterclockwise)

Preceding gene: 83593495

Following gene: 83593490

Centisome position: 57.59

GC content: 63.87

Gene sequence:

>1536_bases
ATGCGCCTTGGGGATTTTTCTCGCTATCTCCCTGGTGGACGGAGGGGTCTCTTCCCTATCTACTGTGGTGTTATAGAGGG
CCGGCCCCGGAATGGCGGGCTGGTACGACCCCACAAAGCAGAAGAGTTCCCATCCCGAATGACCGATGAGACCCTGCCGT
TTATCCTGCTGGTGGAAGACACCCCCGTTCAGGCAAGAACCTATCTCGACTACCTCGACGGCGGCCCTTGGACGATCGAG
CATGTCGAGACCGGCGCGGAAGCCTTGGCGGTTCTGGAGCGGCGCCCCCCCAGCCTGATGCTCCTTGATATCCATTTGCC
CGATATGGATGGCATGGAGATCCTGCGCCGGGTGCGGTCGCTGCCGCTGTCGATGGGGGTGGTGATGATCACCTCGAACG
CCTCGATCAGTCTGGCGGTGGCGGCGATGCAAGCCGGGGCTAATGATTTCCTGGTCAAGCCGTTCAGCCGCGACCGGCTG
ATGGTCACCCTGCGCAACGTCATCGACAATCAGCGTCTGTCGCGCGAGGTCGAGACCCTGCGCGAGAATCTGGGGATCGC
CGCCTTCCAGGGCTTTGTCGGCTCCTCGGCGGCGATGCAGCGGGTTTACCGGCTGATCGAAGCCTCGGCCGCGAGCACGG
CGACGGTTTTTATCACCGGGGAAAGCGGCAGCGGCAAGGAACTGGCGGCCGAGGCCTTGCACCGCTACGGGCCAAGGGTG
AAAGGCCCCTTCGTCGCCCTGAATTGCGGCGCCATCCCGCGCGAGCTGATGGAAAGCGAGGTCTTCGGCCATGTGCGGGG
GTCGTTCACCGGGGCGGTCTCCGACCGGGACGGGGCGGCCGCCCAGGCCAATGGCGGCACCTTGTTCCTTGATGAGATCT
GCGAGATGGATCCCAATCTGCAGACCAAGCTGCTGCGCTTCCTGCAGACCGGCATGGTCCAGAAAGTCGGCGGCGACCGG
CCCGAAAAGGTCGATGTGCGCATCGTCTGCGCGACAAACCGCGATCCTTTGGAAGAGGTCAAGGCCGGACGCTTCCGCGA
GGATCTGTATTACCGTCTGCACGTCATTCCCATCCGCCTGCCGGCCTTGCGCGACCGCGAGGGCGACGTTCTGGAAATCG
CCGATCATTTCCTCGCCAGCATTGCGACCGAGGAAAAGAAGAGCTTTCGCGGCTTTTCGCCGGCGGTGGCCCAGGCCCTG
CTTGCCTATGACTGGCCGGGAAACGTCCGCCAGTTGATCAATGTCCTGCGCAATGTGGTGGTGCTTAACAACGGGCCGGA
AGTGACCTTGGGGATGCTGCCCCAGGCGATCGCCGATGCCGATGGCGAGGCCATCGCGGGCGAGGCGGCCGAAGGCGAGT
CTCCGGCGCTGTTTCGCCAGCAAGACATCGAACCGTTGTGGGTTGTGGAAAAACGCGCCGTCGAGCGGGCGATCGGCGCC
TGCGGCGGCAATATTCCCCGCGCCGCCGCCCTGTTGCGCGTCAGTCCCAGCACGCTGTATCGCAAGAAGCAGGCCTGGGA
AGAGGGCCGGCATTAA

Upstream 100 bases:

>100_bases
CGCGCGGGTGGACGGCGTGGTCAAGGCGGTCACGGCGCCGCGTTCCCTGCCGCCCTCTCGCCGAAATCGCGAAAAATAAG
CAGGGTTTGCCACCTCTAAT

Downstream 100 bases:

>100_bases
AACAAATCCCGAGCCCTGTGGTCCGCAGCGTTCGTGATCTCTAGCGAGAGGCGAGGGTCTGATCGGCCGCCGGAGTCGCT
TGCGCCATGGTCGCCGGATA

Product: two component, sigma54 specific, Fis family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 511; Mature: 511

Protein sequence:

>511_residues
MRLGDFSRYLPGGRRGLFPIYCGVIEGRPRNGGLVRPHKAEEFPSRMTDETLPFILLVEDTPVQARTYLDYLDGGPWTIE
HVETGAEALAVLERRPPSLMLLDIHLPDMDGMEILRRVRSLPLSMGVVMITSNASISLAVAAMQAGANDFLVKPFSRDRL
MVTLRNVIDNQRLSREVETLRENLGIAAFQGFVGSSAAMQRVYRLIEASAASTATVFITGESGSGKELAAEALHRYGPRV
KGPFVALNCGAIPRELMESEVFGHVRGSFTGAVSDRDGAAAQANGGTLFLDEICEMDPNLQTKLLRFLQTGMVQKVGGDR
PEKVDVRIVCATNRDPLEEVKAGRFREDLYYRLHVIPIRLPALRDREGDVLEIADHFLASIATEEKKSFRGFSPAVAQAL
LAYDWPGNVRQLINVLRNVVVLNNGPEVTLGMLPQAIADADGEAIAGEAAEGESPALFRQQDIEPLWVVEKRAVERAIGA
CGGNIPRAAALLRVSPSTLYRKKQAWEEGRH

Sequences:

>Translated_511_residues
MRLGDFSRYLPGGRRGLFPIYCGVIEGRPRNGGLVRPHKAEEFPSRMTDETLPFILLVEDTPVQARTYLDYLDGGPWTIE
HVETGAEALAVLERRPPSLMLLDIHLPDMDGMEILRRVRSLPLSMGVVMITSNASISLAVAAMQAGANDFLVKPFSRDRL
MVTLRNVIDNQRLSREVETLRENLGIAAFQGFVGSSAAMQRVYRLIEASAASTATVFITGESGSGKELAAEALHRYGPRV
KGPFVALNCGAIPRELMESEVFGHVRGSFTGAVSDRDGAAAQANGGTLFLDEICEMDPNLQTKLLRFLQTGMVQKVGGDR
PEKVDVRIVCATNRDPLEEVKAGRFREDLYYRLHVIPIRLPALRDREGDVLEIADHFLASIATEEKKSFRGFSPAVAQAL
LAYDWPGNVRQLINVLRNVVVLNNGPEVTLGMLPQAIADADGEAIAGEAAEGESPALFRQQDIEPLWVVEKRAVERAIGA
CGGNIPRAAALLRVSPSTLYRKKQAWEEGRH
>Mature_511_residues
MRLGDFSRYLPGGRRGLFPIYCGVIEGRPRNGGLVRPHKAEEFPSRMTDETLPFILLVEDTPVQARTYLDYLDGGPWTIE
HVETGAEALAVLERRPPSLMLLDIHLPDMDGMEILRRVRSLPLSMGVVMITSNASISLAVAAMQAGANDFLVKPFSRDRL
MVTLRNVIDNQRLSREVETLRENLGIAAFQGFVGSSAAMQRVYRLIEASAASTATVFITGESGSGKELAAEALHRYGPRV
KGPFVALNCGAIPRELMESEVFGHVRGSFTGAVSDRDGAAAQANGGTLFLDEICEMDPNLQTKLLRFLQTGMVQKVGGDR
PEKVDVRIVCATNRDPLEEVKAGRFREDLYYRLHVIPIRLPALRDREGDVLEIADHFLASIATEEKKSFRGFSPAVAQAL
LAYDWPGNVRQLINVLRNVVVLNNGPEVTLGMLPQAIADADGEAIAGEAAEGESPALFRQQDIEPLWVVEKRAVERAIGA
CGGNIPRAAALLRVSPSTLYRKKQAWEEGRH

Specific function: Acts negatively to control the expression of luminescence. At low cell density, luxO is phosphorylated, and together with sigma-54, causes repression of the luxCDABEGH operon. This repression could be indirect, luxO could activate a negative regulator of

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790437, Length=458, Percent_Identity=39.5196506550218, Blast_Score=306, Evalue=2e-84,
Organism=Escherichia coli, GI1788550, Length=424, Percent_Identity=39.1509433962264, Blast_Score=296, Evalue=2e-81,
Organism=Escherichia coli, GI1790299, Length=401, Percent_Identity=41.1471321695761, Blast_Score=289, Evalue=3e-79,
Organism=Escherichia coli, GI1788905, Length=452, Percent_Identity=36.7256637168142, Blast_Score=282, Evalue=3e-77,
Organism=Escherichia coli, GI87082117, Length=264, Percent_Identity=42.8030303030303, Blast_Score=218, Evalue=5e-58,
Organism=Escherichia coli, GI87082152, Length=243, Percent_Identity=47.7366255144033, Blast_Score=216, Evalue=3e-57,
Organism=Escherichia coli, GI1789233, Length=323, Percent_Identity=37.4613003095975, Blast_Score=207, Evalue=1e-54,
Organism=Escherichia coli, GI1787583, Length=296, Percent_Identity=39.1891891891892, Blast_Score=199, Evalue=4e-52,
Organism=Escherichia coli, GI1786524, Length=324, Percent_Identity=39.1975308641975, Blast_Score=194, Evalue=9e-51,
Organism=Escherichia coli, GI1789087, Length=284, Percent_Identity=39.0845070422535, Blast_Score=191, Evalue=1e-49,
Organism=Escherichia coli, GI87081872, Length=231, Percent_Identity=41.991341991342, Blast_Score=181, Evalue=8e-47,
Organism=Escherichia coli, GI87081858, Length=359, Percent_Identity=27.5766016713092, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI1789828, Length=261, Percent_Identity=31.8007662835249, Blast_Score=123, Evalue=3e-29,
Organism=Escherichia coli, GI1788394, Length=130, Percent_Identity=28.4615384615385, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI145693157, Length=130, Percent_Identity=29.2307692307692, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1790552, Length=132, Percent_Identity=29.5454545454545, Blast_Score=62, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 56091; Mature: 56091

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLGDFSRYLPGGRRGLFPIYCGVIEGRPRNGGLVRPHKAEEFPSRMTDETLPFILLVED
CCCCCCHHHCCCCCCCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHCCCCCCCEEEEEEC
TPVQARTYLDYLDGGPWTIEHVETGAEALAVLERRPPSLMLLDIHLPDMDGMEILRRVRS
CCCCHHHHHHHHCCCCEEEHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHC
LPLSMGVVMITSNASISLAVAAMQAGANDFLVKPFSRDRLMVTLRNVIDNQRLSREVETL
CCCCCCEEEEECCCCEEEEEEEHHCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHH
RENLGIAAFQGFVGSSAAMQRVYRLIEASAASTATVFITGESGSGKELAAEALHRYGPRV
HHHCCHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHCCCCC
KGPFVALNCGAIPRELMESEVFGHVRGSFTGAVSDRDGAAAQANGGTLFLDEICEMDPNL
CCCEEEEECCCCHHHHHHHHHHHHHCCCEEECCCCCCCCCEECCCCEEEHHHHHCCCCCH
QTKLLRFLQTGMVQKVGGDRPEKVDVRIVCATNRDPLEEVKAGRFREDLYYRLHVIPIRL
HHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCHHHHHHCCCHHHHHEEEEEEEEEEC
PALRDREGDVLEIADHFLASIATEEKKSFRGFSPAVAQALLAYDWPGNVRQLINVLRNVV
CCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHHHHHHCEE
VLNNGPEVTLGMLPQAIADADGEAIAGEAAEGESPALFRQQDIEPLWVVEKRAVERAIGA
EECCCCCEEHHHCCHHHHCCCCCEECCCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHH
CGGNIPRAAALLRVSPSTLYRKKQAWEEGRH
CCCCCCHHHHEEEECHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRLGDFSRYLPGGRRGLFPIYCGVIEGRPRNGGLVRPHKAEEFPSRMTDETLPFILLVED
CCCCCCHHHCCCCCCCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHCCCCCCCEEEEEEC
TPVQARTYLDYLDGGPWTIEHVETGAEALAVLERRPPSLMLLDIHLPDMDGMEILRRVRS
CCCCHHHHHHHHCCCCEEEHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHC
LPLSMGVVMITSNASISLAVAAMQAGANDFLVKPFSRDRLMVTLRNVIDNQRLSREVETL
CCCCCCEEEEECCCCEEEEEEEHHCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHH
RENLGIAAFQGFVGSSAAMQRVYRLIEASAASTATVFITGESGSGKELAAEALHRYGPRV
HHHCCHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHCCCCC
KGPFVALNCGAIPRELMESEVFGHVRGSFTGAVSDRDGAAAQANGGTLFLDEICEMDPNL
CCCEEEEECCCCHHHHHHHHHHHHHCCCEEECCCCCCCCCEECCCCEEEHHHHHCCCCCH
QTKLLRFLQTGMVQKVGGDRPEKVDVRIVCATNRDPLEEVKAGRFREDLYYRLHVIPIRL
HHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCHHHHHHCCCHHHHHEEEEEEEEEEC
PALRDREGDVLEIADHFLASIATEEKKSFRGFSPAVAQALLAYDWPGNVRQLINVLRNVV
CCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHHHHHHCEE
VLNNGPEVTLGMLPQAIADADGEAIAGEAAEGESPALFRQQDIEPLWVVEKRAVERAIGA
EECCCCCEEHHHCCHHHHCCCCCEECCCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHH
CGGNIPRAAALLRVSPSTLYRKKQAWEEGRH
CCCCCCHHHHEEEECHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8065259 [H]