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The map label for this gene is sbp [H]

Identifier: 83593409

GI number: 83593409

Start: 2400646

End: 2401740

Strand: Reverse

Name: sbp [H]

Synonym: Rru_A2074

Alternate gene names: 83593409

Gene position: 2401740-2400646 (Counterclockwise)

Preceding gene: 83593412

Following gene: 83593407

Centisome position: 55.18

GC content: 68.77

Gene sequence:

>1095_bases
ATGGCGACGACGCGAAACGAGGCGACAGGGAAGATCCGGGCCTGGGGTGGCCTTATCCTCCGTCTGTCGATCATGGTCCT
GCTGATCGGTGCCGCCCTGTGGGTTGTCGCCGACACGCCAAGCCGGGCCTTGATCAATGTCTCCTATGATCCGACCCGCG
AGTTCTACGCCGATTTCGATGCCGCCTTCACCGCCGCCTGGAAGCGCGAGACCGGCCAGACCGTGGTGATCCGCAGCCTG
CACGGCGGCTCGGGCGCCCAGGCCGACAGCGTCATCGCCGGCGAGGAGGCCGATGTGGTGACCCTGGCCCTGGCCCAAGA
TATCGACACGCTGAGCGCGGCGGGGCTGGTGGCCAAGGGCTGGCAGGCGCGGCTGCCCTATGACAGCTCGCCCTATACCT
CGACCATCGTTTTCCTGGTGCGCAAGGGCAACCCCAAGGCCATCACCGATTGGGACGACCTGCTGCGCCCGGGGGTCGGC
GTGGTCACCGCCAATCCCAAAACCTCGGGCGGCGGGCGCTGGGCCTTCCTCGCCGCCTGGGCCTGGGCGGCGCGGGCGGC
GGCAAACGGCGAAGACCCCGATCGGGCGGCCGAAGCCTATGTCAGCGCGCTGTATCGCCATGTGGTGGCCCTCGACGACG
GCGCCCGGGGAGCGACCTTGTCCTTTACCGAGGGCGGGCTTGGCGACGTGTTGCTGGCCTGGGAGAACGAGGCCTTCCTG
GCGCTTGAAGAGCACGGCGACGAGGCGGAGGGCGGCTTCGAGATTGTCGTGCCCAGCCTCTCCATCCTGGCCCAGCCGAC
GGTGGCGGTGGTCGATCGCAATGTCGATCGCCACGAGACCCGGCCGCTCGCCGAGGCCTATCTCGCCTATCTGTATTCCC
CCGACGGCCAGCGTCTGGCCGCCCGCCACCACTACCGGCCGCTGCGCCCCGAACAGGCCGATTGGGACGACCGCGCCCGC
TTCGCCAGCTTGCGGTTGGTTACGGTCGGCGAAGCCTTCGGCGGGTGGAAAGTGGCCCAGCCGCGGTTCTTTGACGAGGG
CGGGGTGTTCGACCGCATCCACGCCGCCATCGGCCCCATTCCTCCAAAGGAATAG

Upstream 100 bases:

>100_bases
AGGGCGTCGGTCATGGGCGATCCTTATCAAACGGCGAAGGGAATTGTAGACAAACCCGCTGGAGTTTACTGTTCTAAGGG
CCCCAGGGGGAAGGATAGCG

Downstream 100 bases:

>100_bases
GGCAAATCCCGAGCACTCCGAATCAGAGTGCGACGACGATTTGCTTGACCATCAAGATCTGAGAGCGGTGAGCGGGAACC
AGAGTTCCCCGCTCACCGCT

Product: thiosulfate-binding protein

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: Sulfate starvation-induced protein 2; SSI2 [H]

Number of amino acids: Translated: 364; Mature: 363

Protein sequence:

>364_residues
MATTRNEATGKIRAWGGLILRLSIMVLLIGAALWVVADTPSRALINVSYDPTREFYADFDAAFTAAWKRETGQTVVIRSL
HGGSGAQADSVIAGEEADVVTLALAQDIDTLSAAGLVAKGWQARLPYDSSPYTSTIVFLVRKGNPKAITDWDDLLRPGVG
VVTANPKTSGGGRWAFLAAWAWAARAAANGEDPDRAAEAYVSALYRHVVALDDGARGATLSFTEGGLGDVLLAWENEAFL
ALEEHGDEAEGGFEIVVPSLSILAQPTVAVVDRNVDRHETRPLAEAYLAYLYSPDGQRLAARHHYRPLRPEQADWDDRAR
FASLRLVTVGEAFGGWKVAQPRFFDEGGVFDRIHAAIGPIPPKE

Sequences:

>Translated_364_residues
MATTRNEATGKIRAWGGLILRLSIMVLLIGAALWVVADTPSRALINVSYDPTREFYADFDAAFTAAWKRETGQTVVIRSL
HGGSGAQADSVIAGEEADVVTLALAQDIDTLSAAGLVAKGWQARLPYDSSPYTSTIVFLVRKGNPKAITDWDDLLRPGVG
VVTANPKTSGGGRWAFLAAWAWAARAAANGEDPDRAAEAYVSALYRHVVALDDGARGATLSFTEGGLGDVLLAWENEAFL
ALEEHGDEAEGGFEIVVPSLSILAQPTVAVVDRNVDRHETRPLAEAYLAYLYSPDGQRLAARHHYRPLRPEQADWDDRAR
FASLRLVTVGEAFGGWKVAQPRFFDEGGVFDRIHAAIGPIPPKE
>Mature_363_residues
ATTRNEATGKIRAWGGLILRLSIMVLLIGAALWVVADTPSRALINVSYDPTREFYADFDAAFTAAWKRETGQTVVIRSLH
GGSGAQADSVIAGEEADVVTLALAQDIDTLSAAGLVAKGWQARLPYDSSPYTSTIVFLVRKGNPKAITDWDDLLRPGVGV
VTANPKTSGGGRWAFLAAWAWAARAAANGEDPDRAAEAYVSALYRHVVALDDGARGATLSFTEGGLGDVLLAWENEAFLA
LEEHGDEAEGGFEIVVPSLSILAQPTVAVVDRNVDRHETRPLAEAYLAYLYSPDGQRLAARHHYRPLRPEQADWDDRARF
ASLRLVTVGEAFGGWKVAQPRFFDEGGVFDRIHAAIGPIPPKE

Specific function: This protein specifically binds sulfate and is involved in its transmembrane transport [H]

COG id: COG1613

COG function: function code P; ABC-type sulfate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic sulfate-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1790351, Length=310, Percent_Identity=57.0967741935484, Blast_Score=352, Evalue=3e-98,
Organism=Escherichia coli, GI1788765, Length=315, Percent_Identity=40.9523809523809, Blast_Score=229, Evalue=2e-61,

Paralogues:

None

Copy number: 1240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR000957
- InterPro:   IPR005669 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 39279; Mature: 39148

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
0.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
0.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATTRNEATGKIRAWGGLILRLSIMVLLIGAALWVVADTPSRALINVSYDPTREFYADFD
CCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHEEEECCCCCEEEEECCCCCHHHHHHHH
AAFTAAWKRETGQTVVIRSLHGGSGAQADSVIAGEEADVVTLALAQDIDTLSAAGLVAKG
HHHHHHHHCCCCCEEEEEEECCCCCCCCCCEECCCCCCEEEEEEHHCCHHHHHCCHHHCC
WQARLPYDSSPYTSTIVFLVRKGNPKAITDWDDLLRPGVGVVTANPKTSGGGRWAFLAAW
CEEECCCCCCCCCEEEEEEEECCCCCEECCHHHHHCCCCEEEECCCCCCCCCCEEHHHHH
AWAARAAANGEDPDRAAEAYVSALYRHVVALDDGARGATLSFTEGGLGDVLLAWENEAFL
HHHHHHCCCCCCHHHHHHHHHHHHHHHHEEECCCCCCCEEEECCCCCCCEEEEECCCEEE
ALEEHGDEAEGGFEIVVPSLSILAQPTVAVVDRNVDRHETRPLAEAYLAYLYSPDGQRLA
EEECCCCCCCCCEEEEECCHHHHCCCCEEEEECCCCHHHCCHHHHHHHHHHCCCCCCHHH
ARHHYRPLRPEQADWDDRARFASLRLVTVGEAFGGWKVAQPRFFDEGGVFDRIHAAIGPI
HHHCCCCCCCCCCCCHHHHHEEEEEEEEECCCCCCEEECCCCCCCCCCCHHHHHHHHCCC
PPKE
CCCC
>Mature Secondary Structure 
ATTRNEATGKIRAWGGLILRLSIMVLLIGAALWVVADTPSRALINVSYDPTREFYADFD
CCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHEEEECCCCCEEEEECCCCCHHHHHHHH
AAFTAAWKRETGQTVVIRSLHGGSGAQADSVIAGEEADVVTLALAQDIDTLSAAGLVAKG
HHHHHHHHCCCCCEEEEEEECCCCCCCCCCEECCCCCCEEEEEEHHCCHHHHHCCHHHCC
WQARLPYDSSPYTSTIVFLVRKGNPKAITDWDDLLRPGVGVVTANPKTSGGGRWAFLAAW
CEEECCCCCCCCCEEEEEEEECCCCCEECCHHHHHCCCCEEEECCCCCCCCCCEEHHHHH
AWAARAAANGEDPDRAAEAYVSALYRHVVALDDGARGATLSFTEGGLGDVLLAWENEAFL
HHHHHHCCCCCCHHHHHHHHHHHHHHHHEEECCCCCCCEEEECCCCCCCEEEEECCCEEE
ALEEHGDEAEGGFEIVVPSLSILAQPTVAVVDRNVDRHETRPLAEAYLAYLYSPDGQRLA
EEECCCCCCCCCEEEEECCHHHHCCCCEEEEECCCCHHHCCHHHHHHHHHHCCCCCCHHH
ARHHYRPLRPEQADWDDRARFASLRLVTVGEAFGGWKVAQPRFFDEGGVFDRIHAAIGPI
HHHCCCCCCCCCCCCHHHHHEEEEEEEEECCCCCCEEECCCCCCCCCCCHHHHHHHHCCC
PPKE
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3158524; 2002055; 8346018; 9278503; 9298646; 8774726 [H]