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The map label for this gene is aspS

Identifier: 83593407

GI number: 83593407

Start: 2397395

End: 2399215

Strand: Reverse

Name: aspS

Synonym: Rru_A2072

Alternate gene names: 83593407

Gene position: 2399215-2397395 (Counterclockwise)

Preceding gene: 83593409

Following gene: 83593404

Centisome position: 55.12

GC content: 63.7

Gene sequence:

>1821_bases
ATGCATCGTTACCGCACCCACACCTGCGAGCAGCTGCGCCCCGAAAACGTGGGCGAAACCGTGCGCCTGTCTGGCTGGGT
CCATCGCAAACGCGACCACGGCAATTTGCTGTTTGTCGACCTGCGCGACCATTACGGCCTGACCCAGGTGGTTTGCGATG
TTTCGAGCGCGGTTTTCAAGACCCTGGAGGCGGTTCGGGCGGAGAGCGTCGTTTCGGTGACCGGCACCGTCGTGGCGCGC
ACCGCCGAAACCGCCAATGCCAAGCTGCCCACCGGCGCCATCGAGGTCAAGGCCCAAACCGTCGAGGTCCTGTCGCAATC
GGAAGTTCTGCCCATGCAGGTCGCCGGCGATCAGGAGTACCCCGAGGACATGCGGCTGACCTACCGCTTCCTTGATCTGC
GCCGCGAGGACGTCCATGCCAATATGGTTCTGCGCAGCAAGGTGATCTCGTCGCTGCGCCGGCGGATGACCGATCAGGGC
TTCTTGGAATATCAGACGCCGATCCTGACGGCGTCGAGCCCTGAAGGTGCGCGCGACTTCCTGGTGCCCTCGCGCATCCA
TCCGGGCAAGTTCTACGCCTTGCCCCAGGCCCCCCAGCAGTTCAAGCAGATGCTGATGGTCAGCGGCTTTGACCGCTATT
TCCAGGTCGCGCCTTGCTTCCGCGACGAGGACAGCCGCGCCGATCGCAGCCCCGGCGAGTTCTATCAGCTTGATTTCGAG
ATGAGCTTCGTTACCCAGGAAGACGTTTTCGCCGCGATCGAGCCGGTGCTGGTCGGCGTGTTCGAGGAATTCGGCGGCGA
TCGCGCCGTGACCCCGGCGCCGTTCCCGCGCATCCCCTATGACGAGGCGATGCTGAAGTTCGGCAGCGACAAGCCTGACC
TGCGCAATCCCTTGCGCAACGCCGATGTCACCGAGCATTTCGCCGATGGCGGCTTCGGGCTGTTTTCCAAGCAGATCGCC
CTGGGCAGCGTCGTGCGCGCCATTCCCGCCCCCGGCGCCGCCGGCCGGCCGCGCGGCTGGTATGACAAGCTCAACGAATG
GGCCCGCGAAAGCGGCGCCGGTGGTCTCGGCTATGTCGTCTTCGAGGAGGCCGGTCCCAAGGGTCCGATCGCCAAGAACC
TGGAGCCCGAGCGCGTCGAGGCGATCCGTCAGGCCCTCGACCTCAAGGCCGGCGACAGCGTGTTCTTCGCCTGCGACAAG
CCGCTGAAGGCCGCCAAGTTCTCGGGCCTTGTGCGCGAGCGTATCTGCGACGACCTGGGTCTGCGCGAGACCGGCGTTTT
CCGCTTCTGCTGGACCGTCGATTTCCCGATGTACGAGATGAACGAGGAAACCGGCCAGATCGAGTTCAGCCACAACCCGT
TCTCGATGCCCCAGGGCGAGATGGAAGCCCTGGAGACCATGGATCCGCTGGCGATCAAGGCCTATCAGTACGACATCGTG
TGCAACGGCATCGAACTGTCGTCGGGCGCCATCCGGAACCATCTGCCCGAAATCATGTACAAGGCCTTTGAGATCGCCGG
TTATCCCAAGGAAGAGGTGGAAGCCCGTTTCGGCGGCATGCTGCGCGCCTTCAAGTTCGGCGCCCCGCCCCATGGCGGCT
CGGCCCCGGGCATCGACCGCATCGTCATGCTGCTGGCCGACGAGCCCAATATCCGCGAAGTCATCTTGTTCCCGATGAAC
GGTCAGGCCCAGGATCTGCTGATGCAGGCCCCCAATACGGTCAGCGACAAGCAGCTCAAAGAACTGCACATCAAGCTTGA
TCTGCCCAAAAAGGCGCTGAAGGTCGACAAGGGCGAGGCGGCCGGCGACAAGGCCGAATAA

Upstream 100 bases:

>100_bases
CGCGGTCGGCAAGGGGGTGCCTCGCCGACTTGACAAAAGGCGCTCAAGGGTGTCCCTTCCGGTACCCTTTCCGGCCCCCG
TTTGACCAGGATTCTCGACC

Downstream 100 bases:

>100_bases
GCCGTTTCCGTCTTCCCCGCCGCCCCGCTCTCACCCCCGCCCCTTGGAAAAAGGGGCGGGGTTTTTCTTGGTCAGGCTTA
GCGCATGGTCGGGATGACGA

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS

Number of amino acids: Translated: 606; Mature: 606

Protein sequence:

>606_residues
MHRYRTHTCEQLRPENVGETVRLSGWVHRKRDHGNLLFVDLRDHYGLTQVVCDVSSAVFKTLEAVRAESVVSVTGTVVAR
TAETANAKLPTGAIEVKAQTVEVLSQSEVLPMQVAGDQEYPEDMRLTYRFLDLRREDVHANMVLRSKVISSLRRRMTDQG
FLEYQTPILTASSPEGARDFLVPSRIHPGKFYALPQAPQQFKQMLMVSGFDRYFQVAPCFRDEDSRADRSPGEFYQLDFE
MSFVTQEDVFAAIEPVLVGVFEEFGGDRAVTPAPFPRIPYDEAMLKFGSDKPDLRNPLRNADVTEHFADGGFGLFSKQIA
LGSVVRAIPAPGAAGRPRGWYDKLNEWARESGAGGLGYVVFEEAGPKGPIAKNLEPERVEAIRQALDLKAGDSVFFACDK
PLKAAKFSGLVRERICDDLGLRETGVFRFCWTVDFPMYEMNEETGQIEFSHNPFSMPQGEMEALETMDPLAIKAYQYDIV
CNGIELSSGAIRNHLPEIMYKAFEIAGYPKEEVEARFGGMLRAFKFGAPPHGGSAPGIDRIVMLLADEPNIREVILFPMN
GQAQDLLMQAPNTVSDKQLKELHIKLDLPKKALKVDKGEAAGDKAE

Sequences:

>Translated_606_residues
MHRYRTHTCEQLRPENVGETVRLSGWVHRKRDHGNLLFVDLRDHYGLTQVVCDVSSAVFKTLEAVRAESVVSVTGTVVAR
TAETANAKLPTGAIEVKAQTVEVLSQSEVLPMQVAGDQEYPEDMRLTYRFLDLRREDVHANMVLRSKVISSLRRRMTDQG
FLEYQTPILTASSPEGARDFLVPSRIHPGKFYALPQAPQQFKQMLMVSGFDRYFQVAPCFRDEDSRADRSPGEFYQLDFE
MSFVTQEDVFAAIEPVLVGVFEEFGGDRAVTPAPFPRIPYDEAMLKFGSDKPDLRNPLRNADVTEHFADGGFGLFSKQIA
LGSVVRAIPAPGAAGRPRGWYDKLNEWARESGAGGLGYVVFEEAGPKGPIAKNLEPERVEAIRQALDLKAGDSVFFACDK
PLKAAKFSGLVRERICDDLGLRETGVFRFCWTVDFPMYEMNEETGQIEFSHNPFSMPQGEMEALETMDPLAIKAYQYDIV
CNGIELSSGAIRNHLPEIMYKAFEIAGYPKEEVEARFGGMLRAFKFGAPPHGGSAPGIDRIVMLLADEPNIREVILFPMN
GQAQDLLMQAPNTVSDKQLKELHIKLDLPKKALKVDKGEAAGDKAE
>Mature_606_residues
MHRYRTHTCEQLRPENVGETVRLSGWVHRKRDHGNLLFVDLRDHYGLTQVVCDVSSAVFKTLEAVRAESVVSVTGTVVAR
TAETANAKLPTGAIEVKAQTVEVLSQSEVLPMQVAGDQEYPEDMRLTYRFLDLRREDVHANMVLRSKVISSLRRRMTDQG
FLEYQTPILTASSPEGARDFLVPSRIHPGKFYALPQAPQQFKQMLMVSGFDRYFQVAPCFRDEDSRADRSPGEFYQLDFE
MSFVTQEDVFAAIEPVLVGVFEEFGGDRAVTPAPFPRIPYDEAMLKFGSDKPDLRNPLRNADVTEHFADGGFGLFSKQIA
LGSVVRAIPAPGAAGRPRGWYDKLNEWARESGAGGLGYVVFEEAGPKGPIAKNLEPERVEAIRQALDLKAGDSVFFACDK
PLKAAKFSGLVRERICDDLGLRETGVFRFCWTVDFPMYEMNEETGQIEFSHNPFSMPQGEMEALETMDPLAIKAYQYDIV
CNGIELSSGAIRNHLPEIMYKAFEIAGYPKEEVEARFGGMLRAFKFGAPPHGGSAPGIDRIVMLLADEPNIREVILFPMN
GQAQDLLMQAPNTVSDKQLKELHIKLDLPKKALKVDKGEAAGDKAE

Specific function: Unknown

COG id: COG0173

COG function: function code J; Aspartyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI40789249, Length=599, Percent_Identity=37.0617696160267, Blast_Score=366, Evalue=1e-101,
Organism=Homo sapiens, GI45439306, Length=291, Percent_Identity=28.1786941580756, Blast_Score=100, Evalue=5e-21,
Organism=Homo sapiens, GI226491502, Length=251, Percent_Identity=24.7011952191235, Blast_Score=82, Evalue=2e-15,
Organism=Homo sapiens, GI5031815, Length=267, Percent_Identity=26.9662921348315, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI194272210, Length=267, Percent_Identity=26.9662921348315, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI4758762, Length=286, Percent_Identity=25.1748251748252, Blast_Score=76, Evalue=1e-13,
Organism=Escherichia coli, GI1788173, Length=590, Percent_Identity=41.0169491525424, Blast_Score=429, Evalue=1e-121,
Organism=Escherichia coli, GI1789256, Length=319, Percent_Identity=26.0188087774295, Blast_Score=78, Evalue=2e-15,
Organism=Escherichia coli, GI1790571, Length=355, Percent_Identity=25.3521126760563, Blast_Score=77, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI32566633, Length=598, Percent_Identity=35.1170568561873, Blast_Score=313, Evalue=1e-85,
Organism=Caenorhabditis elegans, GI17551876, Length=281, Percent_Identity=29.8932384341637, Blast_Score=95, Evalue=9e-20,
Organism=Caenorhabditis elegans, GI71994340, Length=303, Percent_Identity=27.3927392739274, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17535927, Length=303, Percent_Identity=27.3927392739274, Blast_Score=84, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17535925, Length=303, Percent_Identity=27.3927392739274, Blast_Score=84, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI71984122, Length=299, Percent_Identity=23.7458193979933, Blast_Score=74, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6325153, Length=670, Percent_Identity=30.2985074626866, Blast_Score=238, Evalue=3e-63,
Organism=Saccharomyces cerevisiae, GI6321807, Length=298, Percent_Identity=25.1677852348993, Blast_Score=89, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6323011, Length=295, Percent_Identity=28.135593220339, Blast_Score=86, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6320242, Length=280, Percent_Identity=25, Blast_Score=66, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24584738, Length=602, Percent_Identity=36.046511627907, Blast_Score=326, Evalue=2e-89,
Organism=Drosophila melanogaster, GI17136276, Length=261, Percent_Identity=28.3524904214559, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI19922416, Length=326, Percent_Identity=23.6196319018405, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24640849, Length=262, Percent_Identity=25.9541984732824, Blast_Score=70, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24640851, Length=262, Percent_Identity=25.9541984732824, Blast_Score=70, Evalue=4e-12,
Organism=Drosophila melanogaster, GI19921528, Length=284, Percent_Identity=24.2957746478873, Blast_Score=66, Evalue=8e-11,

Paralogues:

None

Copy number: 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYD_RHORT (Q2RSM3)

Other databases:

- EMBL:   CP000230
- RefSeq:   YP_427159.1
- ProteinModelPortal:   Q2RSM3
- SMR:   Q2RSM3
- STRING:   Q2RSM3
- GeneID:   3835498
- GenomeReviews:   CP000230_GR
- KEGG:   rru:Rru_A2072
- NMPDR:   fig|1085.1.peg.2650
- eggNOG:   COG0173
- HOGENOM:   HBG396032
- OMA:   YQLDVEM
- ProtClustDB:   PRK00476
- BioCyc:   RRUB269796:RRU_A2072-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00044_B
- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020564
- InterPro:   IPR004524
- InterPro:   IPR018153
- InterPro:   IPR002312
- InterPro:   IPR004115
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365
- Gene3D:   G3DSA:3.30.1360.30
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR22594
- PANTHER:   PTHR22594:SF5
- PRINTS:   PR01042
- TIGRFAMs:   TIGR00459

Pfam domain/function: PF02938 GAD; PF00152 tRNA-synt_2; PF01336 tRNA_anti; SSF50249 Nucleic_acid_OB

EC number: =6.1.1.12

Molecular weight: Translated: 67612; Mature: 67612

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHRYRTHTCEQLRPENVGETVRLSGWVHRKRDHGNLLFVDLRDHYGLTQVVCDVSSAVFK
CCCCCCCCHHHCCCCCCCCEEEECCEEEEECCCCCEEEEEECCCCCHHHHHHHHHHHHHH
TLEAVRAESVVSVTGTVVARTAETANAKLPTGAIEVKAQTVEVLSQSEVLPMQVAGDQEY
HHHHHHHHHHHEEHHHEEEEECCCCCCCCCCCEEEEHHHHHHHHHCCCCCEEEECCCCCC
PEDMRLTYRFLDLRREDVHANMVLRSKVISSLRRRMTDQGFLEYQTPILTASSPEGARDF
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCEEECCCCCCCHHC
LVPSRIHPGKFYALPQAPQQFKQMLMVSGFDRYFQVAPCFRDEDSRADRSPGEFYQLDFE
CCCCCCCCCCEEECCCCHHHHHHHHHHHCCHHHEEECCCCCCCCCCCCCCCCCEEEEEEE
MSFVTQEDVFAAIEPVLVGVFEEFGGDRAVTPAPFPRIPYDEAMLKFGSDKPDLRNPLRN
EEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHCCCCCCHHHHHCCC
ADVTEHFADGGFGLFSKQIALGSVVRAIPAPGAAGRPRGWYDKLNEWARESGAGGLGYVV
CCHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEE
FEEAGPKGPIAKNLEPERVEAIRQALDLKAGDSVFFACDKPLKAAKFSGLVRERICDDLG
EECCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHCC
LRETGVFRFCWTVDFPMYEMNEETGQIEFSHNPFSMPQGEMEALETMDPLAIKAYQYDIV
CCCCCEEEEEEEECCCHHCCCCCCCEEEECCCCCCCCCHHHHHHHHCCCCEEEEEEEEEE
CNGIELSSGAIRNHLPEIMYKAFEIAGYPKEEVEARFGGMLRAFKFGAPPHGGSAPGIDR
ECCEECCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHH
IVMLLADEPNIREVILFPMNGQAQDLLMQAPNTVSDKQLKELHIKLDLPKKALKVDKGEA
EEEEEECCCCCEEEEEECCCCCHHHHHHHCCCCCCHHHHEEEEEEECCCHHHHHCCCCCC
AGDKAE
CCCCCC
>Mature Secondary Structure
MHRYRTHTCEQLRPENVGETVRLSGWVHRKRDHGNLLFVDLRDHYGLTQVVCDVSSAVFK
CCCCCCCCHHHCCCCCCCCEEEECCEEEEECCCCCEEEEEECCCCCHHHHHHHHHHHHHH
TLEAVRAESVVSVTGTVVARTAETANAKLPTGAIEVKAQTVEVLSQSEVLPMQVAGDQEY
HHHHHHHHHHHEEHHHEEEEECCCCCCCCCCCEEEEHHHHHHHHHCCCCCEEEECCCCCC
PEDMRLTYRFLDLRREDVHANMVLRSKVISSLRRRMTDQGFLEYQTPILTASSPEGARDF
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCEEECCCCCCCHHC
LVPSRIHPGKFYALPQAPQQFKQMLMVSGFDRYFQVAPCFRDEDSRADRSPGEFYQLDFE
CCCCCCCCCCEEECCCCHHHHHHHHHHHCCHHHEEECCCCCCCCCCCCCCCCCEEEEEEE
MSFVTQEDVFAAIEPVLVGVFEEFGGDRAVTPAPFPRIPYDEAMLKFGSDKPDLRNPLRN
EEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHCCCCCCHHHHHCCC
ADVTEHFADGGFGLFSKQIALGSVVRAIPAPGAAGRPRGWYDKLNEWARESGAGGLGYVV
CCHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEE
FEEAGPKGPIAKNLEPERVEAIRQALDLKAGDSVFFACDKPLKAAKFSGLVRERICDDLG
EECCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHCC
LRETGVFRFCWTVDFPMYEMNEETGQIEFSHNPFSMPQGEMEALETMDPLAIKAYQYDIV
CCCCCEEEEEEEECCCHHCCCCCCCEEEECCCCCCCCCHHHHHHHHCCCCEEEEEEEEEE
CNGIELSSGAIRNHLPEIMYKAFEIAGYPKEEVEARFGGMLRAFKFGAPPHGGSAPGIDR
ECCEECCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHH
IVMLLADEPNIREVILFPMNGQAQDLLMQAPNTVSDKQLKELHIKLDLPKKALKVDKGEA
EEEEEECCCCCEEEEEECCCCCHHHHHHHCCCCCCHHHHEEEEEEECCCHHHHHCCCCCC
AGDKAE
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA