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The map label for this gene is prfB [H]

Identifier: 83593375

GI number: 83593375

Start: 2359390

End: 2360358

Strand: Reverse

Name: prfB [H]

Synonym: Rru_A2040

Alternate gene names: 83593375

Gene position: 2360358-2359390 (Counterclockwise)

Preceding gene: 83593376

Following gene: 83593374

Centisome position: 54.23

GC content: 61.51

Gene sequence:

>969_bases
ATGCGCGAGCGCACCCATCTGGAGCAATCGGTCGCCGGCTATCGCGCCCTTGAGCGCGAGGTCGACGACGCGGCGACGCT
GATCGAACTGGGCGATGCCGAGGACGATGCCGAAAGCATCGCCGAGGGCGAGCGCATGCTGCACAGCGCCGCCCAGAAAG
CCCGCAAGCAGGAGCTTGAAAGCCTGCTGTCGGGCGAAGCCGATCATAACGACGCCTATTTGGAAGTCCATGCCGGGGCC
GGTGGCACCGAGGCCCAGGATTGGGCGCTGATCTTGCTGCGCATGTATACGCGCTGGGCCGAAGCCCACGGCCACAAGGT
GGAATTCCTTGAAGAAAGCGCCGGGGAAGAAGCCGGGATCAAATCGGCGACGATCAAGATCGTCGGCCATAACGCCTATG
GTTGGCTGAAGACCGAAAGCGGCGTGCACCGGCTGGTGCGCATTTCGCCCTTCGACAGCTCGGCGCGGCGCCATACCAGC
TTTTCCTCGATCTGGGTCTATCCGGTGGTCGATGACACCATCGAGATCGATATCGAAGAAAAGGATTGCCGCATCGATAC
CTACCGGGCCTCGGGCGCCGGTGGTCAGCACGTCAACCGGACGGAATCGGCGGTGCGCATCACCCATATTCCGACCGGCA
TCGTCGTGCAGTGCCAATCCGAACGCTCGCAGCACCAGAACAAGGATTATTGCTGGTCGATGCTGCGCGCCCGCTTGTAC
GAGCATGAGCTGCAAAAGCGCGAAGAAAAGACCAAGGCCCTGGAAGACACCAAGACCGATATCGGCTGGGGTCACCAGAT
CCGCTCCTATGTTCTGCAGCCCTATCAGATGATCAAGGACACGCGGACCAACGTTGAAACCTCTGATACCCAGGGCGTGC
TCAACGGCGATCTTGATCAATTCATGGCCGCGTCGCTGGCCGCCCGCATCGTCGGCGCCGGCGACGCCATGGCCGATATC
GCCAAGTAA

Upstream 100 bases:

>100_bases
CATCTTTGACTGGGATAACGCCCTCAGACGCCTGGATGAACTGAACGCCCTTTCCGAGGATCCCAACCTCTGGAACAACG
CCGCCAAGGCCCAGAAGCTG

Downstream 100 bases:

>100_bases
GATCCGGAACGGGGGCGGGGATGGGGTTCGTGTTGTGCCTCCTCGCCCTTCCTTTGGCCTTATGGCTGATCGCCTGTCTG
GCCTTGGTGATCTTCCAGCG

Product: peptide chain release factor 2

Products: NA

Alternate protein names: RF-2 [H]

Number of amino acids: Translated: 322; Mature: 322

Protein sequence:

>322_residues
MRERTHLEQSVAGYRALEREVDDAATLIELGDAEDDAESIAEGERMLHSAAQKARKQELESLLSGEADHNDAYLEVHAGA
GGTEAQDWALILLRMYTRWAEAHGHKVEFLEESAGEEAGIKSATIKIVGHNAYGWLKTESGVHRLVRISPFDSSARRHTS
FSSIWVYPVVDDTIEIDIEEKDCRIDTYRASGAGGQHVNRTESAVRITHIPTGIVVQCQSERSQHQNKDYCWSMLRARLY
EHELQKREEKTKALEDTKTDIGWGHQIRSYVLQPYQMIKDTRTNVETSDTQGVLNGDLDQFMAASLAARIVGAGDAMADI
AK

Sequences:

>Translated_322_residues
MRERTHLEQSVAGYRALEREVDDAATLIELGDAEDDAESIAEGERMLHSAAQKARKQELESLLSGEADHNDAYLEVHAGA
GGTEAQDWALILLRMYTRWAEAHGHKVEFLEESAGEEAGIKSATIKIVGHNAYGWLKTESGVHRLVRISPFDSSARRHTS
FSSIWVYPVVDDTIEIDIEEKDCRIDTYRASGAGGQHVNRTESAVRITHIPTGIVVQCQSERSQHQNKDYCWSMLRARLY
EHELQKREEKTKALEDTKTDIGWGHQIRSYVLQPYQMIKDTRTNVETSDTQGVLNGDLDQFMAASLAARIVGAGDAMADI
AK
>Mature_322_residues
MRERTHLEQSVAGYRALEREVDDAATLIELGDAEDDAESIAEGERMLHSAAQKARKQELESLLSGEADHNDAYLEVHAGA
GGTEAQDWALILLRMYTRWAEAHGHKVEFLEESAGEEAGIKSATIKIVGHNAYGWLKTESGVHRLVRISPFDSSARRHTS
FSSIWVYPVVDDTIEIDIEEKDCRIDTYRASGAGGQHVNRTESAVRITHIPTGIVVQCQSERSQHQNKDYCWSMLRARLY
EHELQKREEKTKALEDTKTDIGWGHQIRSYVLQPYQMIKDTRTNVETSDTQGVLNGDLDQFMAASLAARIVGAGDAMADI
AK

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=295, Percent_Identity=38.9830508474576, Blast_Score=170, Evalue=2e-42,
Organism=Homo sapiens, GI34577120, Length=261, Percent_Identity=37.9310344827586, Blast_Score=150, Evalue=1e-36,
Organism=Homo sapiens, GI166795305, Length=197, Percent_Identity=41.1167512690355, Blast_Score=121, Evalue=8e-28,
Organism=Escherichia coli, GI2367172, Length=307, Percent_Identity=58.957654723127, Blast_Score=363, Evalue=1e-102,
Organism=Escherichia coli, GI1787462, Length=238, Percent_Identity=44.5378151260504, Blast_Score=189, Evalue=2e-49,
Organism=Caenorhabditis elegans, GI17542784, Length=245, Percent_Identity=34.6938775510204, Blast_Score=108, Evalue=4e-24,
Organism=Saccharomyces cerevisiae, GI6321295, Length=243, Percent_Identity=39.0946502057613, Blast_Score=137, Evalue=2e-33,
Organism=Drosophila melanogaster, GI19921226, Length=260, Percent_Identity=38.4615384615385, Blast_Score=153, Evalue=2e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 36022; Mature: 36022

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRERTHLEQSVAGYRALEREVDDAATLIELGDAEDDAESIAEGERMLHSAAQKARKQELE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
SLLSGEADHNDAYLEVHAGAGGTEAQDWALILLRMYTRWAEAHGHKVEFLEESAGEEAGI
HHHCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCC
KSATIKIVGHNAYGWLKTESGVHRLVRISPFDSSARRHTSFSSIWVYPVVDDTIEIDIEE
CEEEEEEEECCCCEEEECCCCCEEEEEECCCCCCHHHCCCCCEEEEEEEECCEEEEEEEC
KDCRIDTYRASGAGGQHVNRTESAVRITHIPTGIVVQCQSERSQHQNKDYCWSMLRARLY
CCCEEEEEECCCCCCCCCCCCCCEEEEEECCCCEEEEECCHHHHCCCHHHHHHHHHHHHH
EHELQKREEKTKALEDTKTDIGWGHQIRSYVLQPYQMIKDTRTNVETSDTQGVLNGDLDQ
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHH
FMAASLAARIVGAGDAMADIAK
HHHHHHHHHHHCCCHHHHHHCC
>Mature Secondary Structure
MRERTHLEQSVAGYRALEREVDDAATLIELGDAEDDAESIAEGERMLHSAAQKARKQELE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
SLLSGEADHNDAYLEVHAGAGGTEAQDWALILLRMYTRWAEAHGHKVEFLEESAGEEAGI
HHHCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCC
KSATIKIVGHNAYGWLKTESGVHRLVRISPFDSSARRHTSFSSIWVYPVVDDTIEIDIEE
CEEEEEEEECCCCEEEECCCCCEEEEEECCCCCCHHHCCCCCEEEEEEEECCEEEEEEEC
KDCRIDTYRASGAGGQHVNRTESAVRITHIPTGIVVQCQSERSQHQNKDYCWSMLRARLY
CCCEEEEEECCCCCCCCCCCCCCEEEEEECCCCEEEEECCHHHHCCCHHHHHHHHHHHHH
EHELQKREEKTKALEDTKTDIGWGHQIRSYVLQPYQMIKDTRTNVETSDTQGVLNGDLDQ
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHH
FMAASLAARIVGAGDAMADIAK
HHHHHHHHHHHCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA