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The map label for this gene is trpB2 [H]

Identifier: 83593314

GI number: 83593314

Start: 2290185

End: 2291543

Strand: Reverse

Name: trpB2 [H]

Synonym: Rru_A1979

Alternate gene names: 83593314

Gene position: 2291543-2290185 (Counterclockwise)

Preceding gene: 83593316

Following gene: 83593313

Centisome position: 52.64

GC content: 63.87

Gene sequence:

>1359_bases
ATGACCGAGAGTACCAAATACCTGCTGTCCGAAGATCGGTTGCCCAAGGCCTGGTACAATCTGGCCGCCGATCTGCCCGT
GCCCCTGCCGCCGCCGCTCAACCCCGGAACGCTGAAGCCATTGGTCGCCGATGATCTGGCGCCGATCTTCCCGCGCGCGG
TGATCGAGCAGGAGATGAGCGCCGAGCGCTGGATCGATATCCCCGAACCGGTGCGCGAGGTGTATCGCCTGTGGCGGCCG
TCGCCGCTGATCCGCGCCCGGCGGCTCGAGCGCGCCCTTGATACCCCCGCCCATATCTACTTCAAATACGAAGGCGTGAG
CCCGGCGGGCAGCCATAAGCCCAATACCTCGGTCCCCCAGGCGTTCTATAACCAGCAAGAGGGCGTCAAGAAGCTGGCGA
CCGAAACCGGCGCCGGCCAATGGGGCTGTTCGCTGGCCTTCGCCGGATCGCTGTTCGGCCTGGAAGTCCAAGTGTTCATG
GTCAAGGTGTCGTTCAATCAGAAGCCCTATCGCCGGGCGCTGATGGAAACCTATGGCGCCACCTGCATTCCCAGCCCCTC
GCCGCTGACCGAGGCCGGCCGCGCCATTCTGGCCCGCGACCCGGAGTCCAACGGCTCGCTCGGCATCGCCATTTCCGAGG
CGGTCGAGGTCGCCATCGCCCATGACGACACCAAATACGCCCTGGGCAGCGTCCTCAATCACGTCTGCCTGCATCAGACG
GTGATCGGCGAAGAAGCGCTGTTACAGATGGAGATGGCCGGCGACGAGCCCGATGTGGTGATCGCCTGCACCGGCGGCGG
CTCGAACTTCGCCGGACTGGCCTTCCCCTATATCCGCGAGAAGATCAAGGGGCGGTCGATCCGCATCGTCGGCGTCGAAC
CCCATTCCTGCCCGACCCTGACCAAGGGCCGCTATGCCTATGATTTCGGCGATACGGCCAAGCTGACGCCCTTGATGAAG
ATGCATACCCTGGGCGCCACCTTCATGCCGCCCGGCTCGCATTCCGGCGGCCTGCGCTACCACGGCATGTCGCCGATGGT
CAGCCACGTCAAGGAACTGGGCCTGATGGAGGCGCGGTCCTATCACCAGACCGAATGCTTCGCCGCCGCCCTGCAATTCG
CCCGCAACGAAGGCATCGTTCCCGCACCCGAGTCTTCGCATGCGGTTAAGGCCGCCATCGACGAAGCCTTGCTGGCCAAG
GCCGAAGGCAAGGCGCGGACGATCCTGTTCAATCTGTCGGGCCACGGCCATTTCGACATGCAGGCCTATACGGATTATTT
GGCCGGAAAGCTCAAGGACCGGGTGTTCGACGACAGTCTGCTTGACGAAGCCTTGGCCGATCTGCCGGTTATCGACTGA

Upstream 100 bases:

>100_bases
CCCGGGCAAGACGATGGTTTCGCGCTCGCGCTTTGCAAGCCCGGCCTCGGAGGGGTAGAACGGGCCGTCCGACGCCTATC
CGTCCAAGGGAAATAACGAG

Downstream 100 bases:

>100_bases
TCCCGATCGCCGGTTTCGGCGTGAAAGCCTCGCCCCCCGGGAGCCCTTGATCGGCGGCTCCCGGGGGGCTTTTTTTCATA
AGACCTTAATTTCTTTATAA

Product: tryptophan synthase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 452; Mature: 451

Protein sequence:

>452_residues
MTESTKYLLSEDRLPKAWYNLAADLPVPLPPPLNPGTLKPLVADDLAPIFPRAVIEQEMSAERWIDIPEPVREVYRLWRP
SPLIRARRLERALDTPAHIYFKYEGVSPAGSHKPNTSVPQAFYNQQEGVKKLATETGAGQWGCSLAFAGSLFGLEVQVFM
VKVSFNQKPYRRALMETYGATCIPSPSPLTEAGRAILARDPESNGSLGIAISEAVEVAIAHDDTKYALGSVLNHVCLHQT
VIGEEALLQMEMAGDEPDVVIACTGGGSNFAGLAFPYIREKIKGRSIRIVGVEPHSCPTLTKGRYAYDFGDTAKLTPLMK
MHTLGATFMPPGSHSGGLRYHGMSPMVSHVKELGLMEARSYHQTECFAAALQFARNEGIVPAPESSHAVKAAIDEALLAK
AEGKARTILFNLSGHGHFDMQAYTDYLAGKLKDRVFDDSLLDEALADLPVID

Sequences:

>Translated_452_residues
MTESTKYLLSEDRLPKAWYNLAADLPVPLPPPLNPGTLKPLVADDLAPIFPRAVIEQEMSAERWIDIPEPVREVYRLWRP
SPLIRARRLERALDTPAHIYFKYEGVSPAGSHKPNTSVPQAFYNQQEGVKKLATETGAGQWGCSLAFAGSLFGLEVQVFM
VKVSFNQKPYRRALMETYGATCIPSPSPLTEAGRAILARDPESNGSLGIAISEAVEVAIAHDDTKYALGSVLNHVCLHQT
VIGEEALLQMEMAGDEPDVVIACTGGGSNFAGLAFPYIREKIKGRSIRIVGVEPHSCPTLTKGRYAYDFGDTAKLTPLMK
MHTLGATFMPPGSHSGGLRYHGMSPMVSHVKELGLMEARSYHQTECFAAALQFARNEGIVPAPESSHAVKAAIDEALLAK
AEGKARTILFNLSGHGHFDMQAYTDYLAGKLKDRVFDDSLLDEALADLPVID
>Mature_451_residues
TESTKYLLSEDRLPKAWYNLAADLPVPLPPPLNPGTLKPLVADDLAPIFPRAVIEQEMSAERWIDIPEPVREVYRLWRPS
PLIRARRLERALDTPAHIYFKYEGVSPAGSHKPNTSVPQAFYNQQEGVKKLATETGAGQWGCSLAFAGSLFGLEVQVFMV
KVSFNQKPYRRALMETYGATCIPSPSPLTEAGRAILARDPESNGSLGIAISEAVEVAIAHDDTKYALGSVLNHVCLHQTV
IGEEALLQMEMAGDEPDVVIACTGGGSNFAGLAFPYIREKIKGRSIRIVGVEPHSCPTLTKGRYAYDFGDTAKLTPLMKM
HTLGATFMPPGSHSGGLRYHGMSPMVSHVKELGLMEARSYHQTECFAAALQFARNEGIVPAPESSHAVKAAIDEALLAKA
EGKARTILFNLSGHGHFDMQAYTDYLAGKLKDRVFDDSLLDEALADLPVID

Specific function: The beta subunit is responsible for the synthesis of L- tryptophan from indole and L-serine [H]

COG id: COG1350

COG function: function code R; Predicted alternative tryptophan synthase beta-subunit (paralog of TrpB)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpB family [H]

Homologues:

Organism=Escherichia coli, GI1787515, Length=369, Percent_Identity=28.9972899728997, Blast_Score=116, Evalue=4e-27,
Organism=Saccharomyces cerevisiae, GI6321412, Length=381, Percent_Identity=30.4461942257218, Blast_Score=117, Evalue=4e-27,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001926
- InterPro:   IPR006316
- InterPro:   IPR006653
- InterPro:   IPR023026 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =4.2.1.20 [H]

Molecular weight: Translated: 49315; Mature: 49184

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: PS00168 TRP_SYNTHASE_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTESTKYLLSEDRLPKAWYNLAADLPVPLPPPLNPGTLKPLVADDLAPIFPRAVIEQEMS
CCCCCCHHHCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
AERWIDIPEPVREVYRLWRPSPLIRARRLERALDTPAHIYFKYEGVSPAGSHKPNTSVPQ
HHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCH
AFYNQQEGVKKLATETGAGQWGCSLAFAGSLFGLEVQVFMVKVSFNQKPYRRALMETYGA
HHHHHHHHHHHHHHHCCCCCCCCHHEECCCCCCEEEEEEEEEEECCCCHHHHHHHHHHCC
TCIPSPSPLTEAGRAILARDPESNGSLGIAISEAVEVAIAHDDTKYALGSVLNHVCLHQT
CCCCCCCCHHHHCCEEEEECCCCCCCEEEEEHHHEEEEEECCCHHHHHHHHHHHHHHHHH
VIGEEALLQMEMAGDEPDVVIACTGGGSNFAGLAFPYIREKIKGRSIRIVGVEPHSCPTL
HHCHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCC
TKGRYAYDFGDTAKLTPLMKMHTLGATFMPPGSHSGGLRYHGMSPMVSHVKELGLMEARS
CCCCEEECCCCCCHHHHHHHHHHCCCEECCCCCCCCCEEECCCCHHHHHHHHHHHHHHHH
YHQTECFAAALQFARNEGIVPAPESSHAVKAAIDEALLAKAEGKARTILFNLSGHGHFDM
HHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCH
QAYTDYLAGKLKDRVFDDSLLDEALADLPVID
HHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TESTKYLLSEDRLPKAWYNLAADLPVPLPPPLNPGTLKPLVADDLAPIFPRAVIEQEMS
CCCCCHHHCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
AERWIDIPEPVREVYRLWRPSPLIRARRLERALDTPAHIYFKYEGVSPAGSHKPNTSVPQ
HHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCH
AFYNQQEGVKKLATETGAGQWGCSLAFAGSLFGLEVQVFMVKVSFNQKPYRRALMETYGA
HHHHHHHHHHHHHHHCCCCCCCCHHEECCCCCCEEEEEEEEEEECCCCHHHHHHHHHHCC
TCIPSPSPLTEAGRAILARDPESNGSLGIAISEAVEVAIAHDDTKYALGSVLNHVCLHQT
CCCCCCCCHHHHCCEEEEECCCCCCCEEEEEHHHEEEEEECCCHHHHHHHHHHHHHHHHH
VIGEEALLQMEMAGDEPDVVIACTGGGSNFAGLAFPYIREKIKGRSIRIVGVEPHSCPTL
HHCHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCC
TKGRYAYDFGDTAKLTPLMKMHTLGATFMPPGSHSGGLRYHGMSPMVSHVKELGLMEARS
CCCCEEECCCCCCHHHHHHHHHHCCCEECCCCCCCCCEEECCCCHHHHHHHHHHHHHHHH
YHQTECFAAALQFARNEGIVPAPESSHAVKAAIDEALLAKAEGKARTILFNLSGHGHFDM
HHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCH
QAYTDYLAGKLKDRVFDDSLLDEALADLPVID
HHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11932238 [H]