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The map label for this gene is leuA

Identifier: 83593263

GI number: 83593263

Start: 2233751

End: 2235427

Strand: Direct

Name: leuA

Synonym: Rru_A1928

Alternate gene names: 83593263

Gene position: 2233751-2235427 (Clockwise)

Preceding gene: 83593261

Following gene: 83593269

Centisome position: 51.32

GC content: 64.34

Gene sequence:

>1677_bases
ATGATGATCGATCCTTCCAAGAAATACCGACCCTTCCCGCCCGTCGCCCTGGCCGACCGCACTTGGCCCAGCGCCACCAT
CACCAAGGCGCCGATCTGGTGCGCCGTCGATCTGCGCGACGGCAATCAGGCGCTGGTCGAACCGATGGGCGCCGATCGCA
AGGTCCGCATGTTCCAGTTGCTGTGCGAGCTGGGCTACAAGGAAATCGAAGTCGGCTTCCCCTCGGCCTCGCAAACCGAT
TTCGACTTCCTGCGCCAATTGATCGAACGCGACATGATCCCGGCCGATGTCACCATCCAGGTGCTGACCCAGGCCCGCGA
AGACCTGATCAGCCGGACCTTCCAGGCGCTCGAGGGCGCGTCTTCGGCCATCGTCCACCTCTATAATTCAACCTCGACCC
TGCAGCGGCGGGTGGTATTCGCCCTCGACCGCGCCGGCATCACCGATCTGGCGGTGCGCGGCGCCGAAATGGTGCGCGAG
GGCGCGGCCAAGGCCTCGGCTTCAACCAAACTGCGCTTCCAATACTCGCCCGAAAGCTTCACCGGCACCGAACTCGATTA
CGCCGTGGAGATCTGCGAGGCGGTGATGGGAGTCTTCGAGCCGACCGCCGAGGCGCCCTTGATCCTCAACCTGCCCTCGA
CGGTCGAGATGGCGACGCCCAATGTCTATGCCGATCAGATCGAATGGTTCTGCCGGGCCCTGCCCCATCGCGACCGAGTG
CTGATCAGCCTGCATCCCCATAACGACCGGGGAACGGCGGTGGCGGCGGCCGAACTGGCGCTGATGGCCGGGGCCGATCG
CATCGAAGGCACGCTGTTTGGCAATGGCGAGCGCACCGGTAACGTCGATCTGGTGACCTTGGGCATGAACATGTTCACCC
AGGGCATCGATCCGGGCATCGATTTCTCCAATATCGATCACATCCGCGAAATCGCCGAGGAATGTAACCGCCTGCCCGTC
CACCCCCGCCACCCCTATGCCGGCGATCTGGTCTTTACTGCCTTCTCGGGCAGCCATCAGGACGCCATCAACAAGGGCCT
GCATGCCATGGAGAAGACCAATCAGGACGTCTGGGAGGTGCCCTATCTGCCGATCGACCCCAAGGACATCGGCCGCAGCT
ATGAAGCGGTGATCCGCGTCAACAGCCAGTCGGGCAAGGGCGGCGTCGCCCATATCCTCGAACGCGATTATGGCATCCGC
ATGCCGCGCGGCCTGCAGGTCGAATTCTCCAAGGTGGTGCAGGCGATCACCGACACCACCGGCGAGGAGATCACCTCGCG
CGGGCTGTGGAAGACCTTCGAGGAAACCTATCTCGGCGCCGGCAGCCCCTATAGCTTCCTGGAACACCACACCCGTCCCG
ATCCGGCCGGCAAGGACCAGCGCATCCTGACCGCCACGGTCAAGGCCGGTGGCAAGGTGCGCGATATCGATGGCCGCGGC
ACCGGTCCGATCGAGGCGCTGGTCGACGCCCTGCGCAAGGACAGCGGTCTGGCGATCACCATCGAGGATTACGAGGAGCA
CACCCTGCGCCCGGGATCGGACGCCCAGGCCGTCGCCTTCATCAAGGCCAGCCTGCCCGATGGCCGCGCCCATTACGGCG
TGGGCATCGACGCCAACTTCGTCGTCGCCTCGCTGAAGGCGGTGCTCTGCGCCGCCAACCACCTGAGCGACAAGTAA

Upstream 100 bases:

>100_bases
GCTTAACGGCCTCCCGGCGGCCGGTTTCCAGGCCATGCACCGCTCTCTCCTCCCCCGTTCGCCGCCATGAAAAGCCCAGC
GCCAAGGAGCGCTTTCCGAG

Downstream 100 bases:

>100_bases
GGCAGGAAGACGGCCCGAGCAAATCCCGAGCCCCCTGGACCAGAGGGCGACGACGATTTGCCTGACGATCAAGATCTGAG
AGCGGCGGACCCACCTTCCA

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase

Number of amino acids: Translated: 558; Mature: 558

Protein sequence:

>558_residues
MMIDPSKKYRPFPPVALADRTWPSATITKAPIWCAVDLRDGNQALVEPMGADRKVRMFQLLCELGYKEIEVGFPSASQTD
FDFLRQLIERDMIPADVTIQVLTQAREDLISRTFQALEGASSAIVHLYNSTSTLQRRVVFALDRAGITDLAVRGAEMVRE
GAAKASASTKLRFQYSPESFTGTELDYAVEICEAVMGVFEPTAEAPLILNLPSTVEMATPNVYADQIEWFCRALPHRDRV
LISLHPHNDRGTAVAAAELALMAGADRIEGTLFGNGERTGNVDLVTLGMNMFTQGIDPGIDFSNIDHIREIAEECNRLPV
HPRHPYAGDLVFTAFSGSHQDAINKGLHAMEKTNQDVWEVPYLPIDPKDIGRSYEAVIRVNSQSGKGGVAHILERDYGIR
MPRGLQVEFSKVVQAITDTTGEEITSRGLWKTFEETYLGAGSPYSFLEHHTRPDPAGKDQRILTATVKAGGKVRDIDGRG
TGPIEALVDALRKDSGLAITIEDYEEHTLRPGSDAQAVAFIKASLPDGRAHYGVGIDANFVVASLKAVLCAANHLSDK

Sequences:

>Translated_558_residues
MMIDPSKKYRPFPPVALADRTWPSATITKAPIWCAVDLRDGNQALVEPMGADRKVRMFQLLCELGYKEIEVGFPSASQTD
FDFLRQLIERDMIPADVTIQVLTQAREDLISRTFQALEGASSAIVHLYNSTSTLQRRVVFALDRAGITDLAVRGAEMVRE
GAAKASASTKLRFQYSPESFTGTELDYAVEICEAVMGVFEPTAEAPLILNLPSTVEMATPNVYADQIEWFCRALPHRDRV
LISLHPHNDRGTAVAAAELALMAGADRIEGTLFGNGERTGNVDLVTLGMNMFTQGIDPGIDFSNIDHIREIAEECNRLPV
HPRHPYAGDLVFTAFSGSHQDAINKGLHAMEKTNQDVWEVPYLPIDPKDIGRSYEAVIRVNSQSGKGGVAHILERDYGIR
MPRGLQVEFSKVVQAITDTTGEEITSRGLWKTFEETYLGAGSPYSFLEHHTRPDPAGKDQRILTATVKAGGKVRDIDGRG
TGPIEALVDALRKDSGLAITIEDYEEHTLRPGSDAQAVAFIKASLPDGRAHYGVGIDANFVVASLKAVLCAANHLSDK
>Mature_558_residues
MMIDPSKKYRPFPPVALADRTWPSATITKAPIWCAVDLRDGNQALVEPMGADRKVRMFQLLCELGYKEIEVGFPSASQTD
FDFLRQLIERDMIPADVTIQVLTQAREDLISRTFQALEGASSAIVHLYNSTSTLQRRVVFALDRAGITDLAVRGAEMVRE
GAAKASASTKLRFQYSPESFTGTELDYAVEICEAVMGVFEPTAEAPLILNLPSTVEMATPNVYADQIEWFCRALPHRDRV
LISLHPHNDRGTAVAAAELALMAGADRIEGTLFGNGERTGNVDLVTLGMNMFTQGIDPGIDFSNIDHIREIAEECNRLPV
HPRHPYAGDLVFTAFSGSHQDAINKGLHAMEKTNQDVWEVPYLPIDPKDIGRSYEAVIRVNSQSGKGGVAHILERDYGIR
MPRGLQVEFSKVVQAITDTTGEEITSRGLWKTFEETYLGAGSPYSFLEHHTRPDPAGKDQRILTATVKAGGKVRDIDGRG
TGPIEALVDALRKDSGLAITIEDYEEHTLRPGSDAQAVAFIKASLPDGRAHYGVGIDANFVVASLKAVLCAANHLSDK

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate)

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 2 subfamily

Homologues:

Organism=Escherichia coli, GI1786261, Length=530, Percent_Identity=25.0943396226415, Blast_Score=112, Evalue=4e-26,
Organism=Saccharomyces cerevisiae, GI6324225, Length=568, Percent_Identity=47.887323943662, Blast_Score=548, Evalue=1e-156,
Organism=Saccharomyces cerevisiae, GI6324682, Length=569, Percent_Identity=48.3304042179262, Blast_Score=543, Evalue=1e-155,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEU1_RHORT (Q2RT17)

Other databases:

- EMBL:   CP000230
- RefSeq:   YP_427015.1
- ProteinModelPortal:   Q2RT17
- SMR:   Q2RT17
- STRING:   Q2RT17
- GeneID:   3835352
- GenomeReviews:   CP000230_GR
- KEGG:   rru:Rru_A1928
- NMPDR:   fig|1085.1.peg.2502
- eggNOG:   COG0119
- HOGENOM:   HBG289752
- OMA:   VERCNQI
- PhylomeDB:   Q2RT17
- ProtClustDB:   PRK03739
- BioCyc:   RRUB269796:RRU_A1928-MONOMER
- HAMAP:   MF_00572
- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005668
- InterPro:   IPR000891
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR10277:SF2
- SMART:   SM00917
- TIGRFAMs:   TIGR00970

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer; SSF110921 2-isopropylmalate_synth_dimer

EC number: =2.3.3.13

Molecular weight: Translated: 61147; Mature: 61147

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1; PS00816 AIPM_HOMOCIT_SYNTH_2; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMIDPSKKYRPFPPVALADRTWPSATITKAPIWCAVDLRDGNQALVEPMGADRKVRMFQL
CCCCCCCCCCCCCCCEECCCCCCCCEEECCCEEEEEEECCCCHHHHCCCCCCCHHHHHHH
LCELGYKEIEVGFPSASQTDFDFLRQLIERDMIPADVTIQVLTQAREDLISRTFQALEGA
HHHCCCEEEEECCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
SSAIVHLYNSTSTLQRRVVFALDRAGITDLAVRGAEMVREGAAKASASTKLRFQYSPESF
CCEEEEEECCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCC
TGTELDYAVEICEAVMGVFEPTAEAPLILNLPSTVEMATPNVYADQIEWFCRALPHRDRV
CCCCHHHHHHHHHHHHHHHCCCCCCCEEEECCCCEEECCCCHHHHHHHHHHHHCCCCCEE
LISLHPHNDRGTAVAAAELALMAGADRIEGTLFGNGERTGNVDLVTLGMNMFTQGIDPGI
EEEEECCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCEEEEEECHHHHHCCCCCCC
DFSNIDHIREIAEECNRLPVHPRHPYAGDLVFTAFSGSHQDAINKGLHAMEKTNQDVWEV
CCCCHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCHHCC
PYLPIDPKDIGRSYEAVIRVNSQSGKGGVAHILERDYGIRMPRGLQVEFSKVVQAITDTT
CCCCCCHHHHCCCEEEEEEEECCCCCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCC
GEEITSRGLWKTFEETYLGAGSPYSFLEHHTRPDPAGKDQRILTATVKAGGKVRDIDGRG
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCCCEEEEEEECCCCEEEECCCCC
TGPIEALVDALRKDSGLAITIEDYEEHTLRPGSDAQAVAFIKASLPDGRAHYGVGIDANF
CCHHHHHHHHHHCCCCCEEEEECCHHHCCCCCCCCCEEEEEEECCCCCCCEECCCCCHHH
VVASLKAVLCAANHLSDK
HHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MMIDPSKKYRPFPPVALADRTWPSATITKAPIWCAVDLRDGNQALVEPMGADRKVRMFQL
CCCCCCCCCCCCCCCEECCCCCCCCEEECCCEEEEEEECCCCHHHHCCCCCCCHHHHHHH
LCELGYKEIEVGFPSASQTDFDFLRQLIERDMIPADVTIQVLTQAREDLISRTFQALEGA
HHHCCCEEEEECCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
SSAIVHLYNSTSTLQRRVVFALDRAGITDLAVRGAEMVREGAAKASASTKLRFQYSPESF
CCEEEEEECCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCC
TGTELDYAVEICEAVMGVFEPTAEAPLILNLPSTVEMATPNVYADQIEWFCRALPHRDRV
CCCCHHHHHHHHHHHHHHHCCCCCCCEEEECCCCEEECCCCHHHHHHHHHHHHCCCCCEE
LISLHPHNDRGTAVAAAELALMAGADRIEGTLFGNGERTGNVDLVTLGMNMFTQGIDPGI
EEEEECCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCEEEEEECHHHHHCCCCCCC
DFSNIDHIREIAEECNRLPVHPRHPYAGDLVFTAFSGSHQDAINKGLHAMEKTNQDVWEV
CCCCHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCHHCC
PYLPIDPKDIGRSYEAVIRVNSQSGKGGVAHILERDYGIRMPRGLQVEFSKVVQAITDTT
CCCCCCHHHHCCCEEEEEEEECCCCCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCC
GEEITSRGLWKTFEETYLGAGSPYSFLEHHTRPDPAGKDQRILTATVKAGGKVRDIDGRG
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCCCEEEEEEECCCCEEEECCCCC
TGPIEALVDALRKDSGLAITIEDYEEHTLRPGSDAQAVAFIKASLPDGRAHYGVGIDANF
CCHHHHHHHHHHCCCCCEEEEECCHHHCCCCCCCCCEEEEEEECCCCCCCEECCCCCHHH
VVASLKAVLCAANHLSDK
HHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA