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The map label for this gene is pdhC [H]

Identifier: 83593214

GI number: 83593214

Start: 2176545

End: 2177867

Strand: Reverse

Name: pdhC [H]

Synonym: Rru_A1879

Alternate gene names: 83593214

Gene position: 2177867-2176545 (Counterclockwise)

Preceding gene: 83593215

Following gene: 83593213

Centisome position: 50.03

GC content: 67.88

Gene sequence:

>1323_bases
ATGCCTATCGAGATCCTGATGCCCGCGCTCTCTCCAACGATGGAGGAGGGAACCCTGGCCAAGTGGCTGAAGAAGGAGGG
CGATCCCATCGCCGCCGGCGATGTCATCGCCGAAATCGAAACCGATAAGGCGACGATGGAATTCGAAGCCACCGACGAGG
GCGTTCTTGGCAAGATCCTGGTCGCCGACGGCACCGCCGGCATCAAGGTCAACCAGCCGATCGGCATTTTGCTTGAGGAA
GGCGAGGACGCCAGCGCCCTGGTCCAGGCCGCTCCGGCCAAAGCCCCGGATGCCCCGGCCAAGGCCGCGCCCGAGCCGGC
CCCTGTCGCCGCGGCGTCCCAAAGCGACGCCGCGCCCGCCCCGGCGGCGGCCGCGCCCAAGCGGGCGGCCGGTGAACGGC
TGTTCGCCAGTCCGCTGGCCCGGCGCATCGCCGCCGCCGAAGGCCTTGATCTGGGAGCCCTTCAAGGCAGCGGCCCCCAT
GGCCGCATCGTGCGCCGCGATGTCGAGGCGGCTTTGGCCGCCGGCACCGGTAAAACGGCCGAAAAGGCGGTTGCCGCGCC
GGTCGCTCCCGCCGCTCCGCCGCAAGCGGTCGCCGCCGCCGCGCCCAAGCCCGTGGCCTTGCCCGACGCGCCCCATACCA
AGGTGGCCAATACGTCGATGCGCAAGATCATCGCCCGGCGCCTGACCGAGTCCAAGCAGACGGTTCCCCATTTCTACCTG
ACCGTCGATTGCAAGATCGACGCCCTGCTTGATCTGCGTAAGAGCTTGAACGCCCGGGCCGAAAAGCGCGGCGACGGGGT
CAAGCTGTCGGTCAACGACCTGATCATCAAGGCCGTCGCCCTCGCCCTGCGCAAGGTGCCCGCCGCCAACGCCTCGTGGA
GCGACGAGGCGATCGTGCTGTGGAGCGATGTCGATATCTCGGTTGCCGTCGCCACCCCGGGCGGGTTGATCACCCCGATC
GTCCGCAAGGCCGACCAGAAGGGGCTGGCGACGATCTCGGCCGAGATGAAGGATCTGGCGACGCGGGCCCGCGACGGCAA
GCTCAAGCCCGAGGAGTTCCAGGGCGGCGGCTTCAGCATCTCCAACCTTGGCATGTACGGCATCCGCGAGTTCGCCGCGA
TCATCAATCCGCCCCAGGGCTGCATCCTGGCCGTCGGCGCCGGCGAACAACGCCCGGTGGTCGAGGCCGGAGCCTTGGCG
ATCGCCACGGTGATGAGTTGCACGCTCTCGGTTGACCATCGGGTGGTCGATGGCGCGGTCGGCGCCGAATTCCTTAGCGC
CTTCAAGATCTTGATCGAAGATCCCATGGCGATGATGCTCTAG

Upstream 100 bases:

>100_bases
GCCAATCTGGAAAAGCTTGCCCTGCCGTCGATCGAGGCGGTCGTCAAGGCGGCGAAGGCCGCCTGCTACAAGTCTTGATC
CGGCGAGAAGGAAGCGCACC

Downstream 100 bases:

>100_bases
TCGCATTCGCCCGTTTCTCTTGCCCACCCGATCGGAAGGGTATTTGCCATGACCGAGACTTCCTTCGATGTGGTCGTTCT
GGGCGCCGGACCCGGCGGCT

Product: dihydrolipoamide acetyltransferase, long form

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 440; Mature: 439

Protein sequence:

>440_residues
MPIEILMPALSPTMEEGTLAKWLKKEGDPIAAGDVIAEIETDKATMEFEATDEGVLGKILVADGTAGIKVNQPIGILLEE
GEDASALVQAAPAKAPDAPAKAAPEPAPVAAASQSDAAPAPAAAAPKRAAGERLFASPLARRIAAAEGLDLGALQGSGPH
GRIVRRDVEAALAAGTGKTAEKAVAAPVAPAAPPQAVAAAAPKPVALPDAPHTKVANTSMRKIIARRLTESKQTVPHFYL
TVDCKIDALLDLRKSLNARAEKRGDGVKLSVNDLIIKAVALALRKVPAANASWSDEAIVLWSDVDISVAVATPGGLITPI
VRKADQKGLATISAEMKDLATRARDGKLKPEEFQGGGFSISNLGMYGIREFAAIINPPQGCILAVGAGEQRPVVEAGALA
IATVMSCTLSVDHRVVDGAVGAEFLSAFKILIEDPMAMML

Sequences:

>Translated_440_residues
MPIEILMPALSPTMEEGTLAKWLKKEGDPIAAGDVIAEIETDKATMEFEATDEGVLGKILVADGTAGIKVNQPIGILLEE
GEDASALVQAAPAKAPDAPAKAAPEPAPVAAASQSDAAPAPAAAAPKRAAGERLFASPLARRIAAAEGLDLGALQGSGPH
GRIVRRDVEAALAAGTGKTAEKAVAAPVAPAAPPQAVAAAAPKPVALPDAPHTKVANTSMRKIIARRLTESKQTVPHFYL
TVDCKIDALLDLRKSLNARAEKRGDGVKLSVNDLIIKAVALALRKVPAANASWSDEAIVLWSDVDISVAVATPGGLITPI
VRKADQKGLATISAEMKDLATRARDGKLKPEEFQGGGFSISNLGMYGIREFAAIINPPQGCILAVGAGEQRPVVEAGALA
IATVMSCTLSVDHRVVDGAVGAEFLSAFKILIEDPMAMML
>Mature_439_residues
PIEILMPALSPTMEEGTLAKWLKKEGDPIAAGDVIAEIETDKATMEFEATDEGVLGKILVADGTAGIKVNQPIGILLEEG
EDASALVQAAPAKAPDAPAKAAPEPAPVAAASQSDAAPAPAAAAPKRAAGERLFASPLARRIAAAEGLDLGALQGSGPHG
RIVRRDVEAALAAGTGKTAEKAVAAPVAPAAPPQAVAAAAPKPVALPDAPHTKVANTSMRKIIARRLTESKQTVPHFYLT
VDCKIDALLDLRKSLNARAEKRGDGVKLSVNDLIIKAVALALRKVPAANASWSDEAIVLWSDVDISVAVATPGGLITPIV
RKADQKGLATISAEMKDLATRARDGKLKPEEFQGGGFSISNLGMYGIREFAAIINPPQGCILAVGAGEQRPVVEAGALAI
ATVMSCTLSVDHRVVDGAVGAEFLSAFKILIEDPMAMML

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI31711992, Length=445, Percent_Identity=44.9438202247191, Blast_Score=365, Evalue=1e-101,
Organism=Homo sapiens, GI203098753, Length=463, Percent_Identity=42.548596112311, Blast_Score=315, Evalue=4e-86,
Organism=Homo sapiens, GI203098816, Length=463, Percent_Identity=42.548596112311, Blast_Score=315, Evalue=5e-86,
Organism=Homo sapiens, GI260898739, Length=161, Percent_Identity=49.0683229813665, Blast_Score=144, Evalue=2e-34,
Organism=Homo sapiens, GI110671329, Length=446, Percent_Identity=26.457399103139, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI19923748, Length=223, Percent_Identity=34.0807174887892, Blast_Score=128, Evalue=8e-30,
Organism=Escherichia coli, GI1786946, Length=438, Percent_Identity=34.4748858447489, Blast_Score=220, Evalue=2e-58,
Organism=Escherichia coli, GI1786305, Length=428, Percent_Identity=32.2429906542056, Blast_Score=162, Evalue=3e-41,
Organism=Caenorhabditis elegans, GI17560088, Length=454, Percent_Identity=45.374449339207, Blast_Score=347, Evalue=7e-96,
Organism=Caenorhabditis elegans, GI17538894, Length=229, Percent_Identity=46.2882096069869, Blast_Score=192, Evalue=3e-49,
Organism=Caenorhabditis elegans, GI25146366, Length=435, Percent_Identity=31.9540229885057, Blast_Score=181, Evalue=9e-46,
Organism=Caenorhabditis elegans, GI17537937, Length=430, Percent_Identity=28.3720930232558, Blast_Score=165, Evalue=4e-41,
Organism=Saccharomyces cerevisiae, GI6324258, Length=460, Percent_Identity=42.8260869565217, Blast_Score=320, Evalue=3e-88,
Organism=Saccharomyces cerevisiae, GI6320352, Length=440, Percent_Identity=31.8181818181818, Blast_Score=174, Evalue=4e-44,
Organism=Saccharomyces cerevisiae, GI6321632, Length=176, Percent_Identity=32.3863636363636, Blast_Score=81, Evalue=3e-16,
Organism=Drosophila melanogaster, GI20129315, Length=454, Percent_Identity=44.7136563876652, Blast_Score=319, Evalue=3e-87,
Organism=Drosophila melanogaster, GI24582497, Length=443, Percent_Identity=44.2437923250564, Blast_Score=303, Evalue=1e-82,
Organism=Drosophila melanogaster, GI18859875, Length=435, Percent_Identity=29.1954022988506, Blast_Score=143, Evalue=2e-34,
Organism=Drosophila melanogaster, GI24645909, Length=222, Percent_Identity=35.1351351351351, Blast_Score=125, Evalue=6e-29,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR006257
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 45284; Mature: 45153

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPIEILMPALSPTMEEGTLAKWLKKEGDPIAAGDVIAEIETDKATMEFEATDEGVLGKIL
CCCEEEECCCCCCCCCCHHHHHHHHCCCCEECCCEEEEEECCCEEEEEECCCCCCEEEEE
VADGTAGIKVNQPIGILLEEGEDASALVQAAPAKAPDAPAKAAPEPAPVAAASQSDAAPA
EECCCCCEEECCCEEEEEECCCCHHHHHHHCCCCCCCCCCCCCCCCCCEECCCCCCCCCC
PAAAAPKRAAGERLFASPLARRIAAAEGLDLGALQGSGPHGRIVRRDVEAALAAGTGKTA
CCCCCCCHHHCHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCHHHHHHHHHHHCCCCCHH
EKAVAAPVAPAAPPQAVAAAAPKPVALPDAPHTKVANTSMRKIIARRLTESKQTVPHFYL
HHHHHCCCCCCCCCHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEE
TVDCKIDALLDLRKSLNARAEKRGDGVKLSVNDLIIKAVALALRKVPAANASWSDEAIVL
EEECCHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCEEEE
WSDVDISVAVATPGGLITPIVRKADQKGLATISAEMKDLATRARDGKLKPEEFQGGGFSI
EECCCEEEEEECCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCHHCCCCCEEH
SNLGMYGIREFAAIINPPQGCILAVGAGEQRPVVEAGALAIATVMSCTLSVDHRVVDGAV
HCCCHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCEEHHHHH
GAEFLSAFKILIEDPMAMML
HHHHHHHHHHHHHCCHHHCC
>Mature Secondary Structure 
PIEILMPALSPTMEEGTLAKWLKKEGDPIAAGDVIAEIETDKATMEFEATDEGVLGKIL
CCEEEECCCCCCCCCCHHHHHHHHCCCCEECCCEEEEEECCCEEEEEECCCCCCEEEEE
VADGTAGIKVNQPIGILLEEGEDASALVQAAPAKAPDAPAKAAPEPAPVAAASQSDAAPA
EECCCCCEEECCCEEEEEECCCCHHHHHHHCCCCCCCCCCCCCCCCCCEECCCCCCCCCC
PAAAAPKRAAGERLFASPLARRIAAAEGLDLGALQGSGPHGRIVRRDVEAALAAGTGKTA
CCCCCCCHHHCHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCHHHHHHHHHHHCCCCCHH
EKAVAAPVAPAAPPQAVAAAAPKPVALPDAPHTKVANTSMRKIIARRLTESKQTVPHFYL
HHHHHCCCCCCCCCHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEE
TVDCKIDALLDLRKSLNARAEKRGDGVKLSVNDLIIKAVALALRKVPAANASWSDEAIVL
EEECCHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCEEEE
WSDVDISVAVATPGGLITPIVRKADQKGLATISAEMKDLATRARDGKLKPEEFQGGGFSI
EECCCEEEEEECCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCHHCCCCCEEH
SNLGMYGIREFAAIINPPQGCILAVGAGEQRPVVEAGALAIATVMSCTLSVDHRVVDGAV
HCCCHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCEEHHHHH
GAEFLSAFKILIEDPMAMML
HHHHHHHHHHHHHCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA