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The map label for this gene is pdhB [H]

Identifier: 83593215

GI number: 83593215

Start: 2177890

End: 2179296

Strand: Reverse

Name: pdhB [H]

Synonym: Rru_A1880

Alternate gene names: 83593215

Gene position: 2179296-2177890 (Counterclockwise)

Preceding gene: 83593216

Following gene: 83593214

Centisome position: 50.07

GC content: 65.25

Gene sequence:

>1407_bases
ATGCCCGTACAGATTCTGATGCCCGCCCTGTCCCCGACGATGACCGAGGGGACCTTGGCCAAATGGCTGAAAAAAGAAGG
CGATACCATCGCCGCCGGCGATGTGATCGCCGAGATCGAAACCGATAAGGCGACCATGGAGTTCGAGGCCGTCGACGAGG
GCGTCCTCGGCCAGATCCTGATCGAGGCCGGAACCCAGAACGTCCCGGTCAACGCCCCGATCGGCATTCTGCTCGAGGAG
GGGGAAACCATCGACGACGTTCACAAGCCCTCGGCCTCGGCCCCGGCCCCGGCCAAGGACGTCTCCTTGGAGACGACGCC
CGCTCCGGCCGAGCCCAGGCGCGACCCGGTTCCCGCCGATACGCCGCTGGCGGCGGGCGGGCGGGCCGAGGCCGGACGCC
AGGAGGAAAAGCGCTACGATAAATTCGTGACGCTGTCGGTGCGCGAGGCCCTGCGCGATGCCATGGCCGAGGAAATGCGC
CGCGACGACAAGGTTTTCCTGCTCGGCGAGGAAGTCGCCCAATACCAGGGCGCCTATAAGATCTCGCAAGGGCTGCTTGA
CGAATTCGGCGAGAAGCGGGTGATCGACACGCCGATCACCGAGATGGGCTTCGCCGGTCTGGCCACCGGCGCGGCGTTTT
CCGGGCTGCGGCCGATCGTCGAATTCATGACCTTCAATTTCTCGATGCAGGCCATCGACCAGATCATCAATTCGGCGGCC
AAGACCCTTTACATGTCGGGCGGTCAGATGGGCTGTCCGATCGTCTTCCGCGGCCCCAATGGCGCCGCCGCCCGCGTCGG
CGCCCAGCACAGCCAGTGCTACGCCAGCTGGTATGCCCATTGCCCCGGCCTGAAGGTGATCGCGCCGTGGAGCGCGGCCG
ATGCCAAGGGCCTGCTGAAGGCGGCGATCCGCGATCCCAATCCGGTGGTCTTCCTGGAAAACGAGATCCTTTACGGCCAG
ACCTTCGAGGTTCCCGACGATGCCGATTTCGTGCTGCCGATCGGCAAGGCCAAGGTGGAACGCGCCGGAGCGGACGTGAC
CCTGGTCGCCTTCTCGCGCATGGTCGGCGTCGCCCTGGAGGCGGCCAAGGCCCTGGCCGGCGAGGGGATCGAGGCCGAGG
TGATCAACCTGCGGACCATCCGTCCGCTTGATGTTGAAACCATCCTCTCCTCGGTGCGCAAGACCAACCGCTGCGTCACC
TTGGAGGAGGGCTGGCCCTTCGCCGGCATCGGCGCCGAGATCGGCATGACCATCATGGAAAACGCCTTCGATTATCTCGA
CGCCCCGGTCATCCGCATCACCGGCGAAGACGTGCCGATGCCCTATGCGGCCAATCTGGAAAAGCTTGCCCTGCCGTCGA
TCGAGGCGGTCGTCAAGGCGGCGAAGGCCGCCTGCTACAAGTCTTGA

Upstream 100 bases:

>100_bases
CAAGCCGCCGAATTCGCCCAGAACAGCCCCGAGCCCGACGCGGCCGAGCTGTACACCGACGTTCTCGTCGAAGCCTGATC
GTCCTCCGGGAGCGCCAACC

Downstream 100 bases:

>100_bases
TCCGGCGAGAAGGAAGCGCACCATGCCTATCGAGATCCTGATGCCCGCGCTCTCTCCAACGATGGAGGAGGGAACCCTGG
CCAAGTGGCTGAAGAAGGAG

Product: pyruvate dehydrogenase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 468; Mature: 467

Protein sequence:

>468_residues
MPVQILMPALSPTMTEGTLAKWLKKEGDTIAAGDVIAEIETDKATMEFEAVDEGVLGQILIEAGTQNVPVNAPIGILLEE
GETIDDVHKPSASAPAPAKDVSLETTPAPAEPRRDPVPADTPLAAGGRAEAGRQEEKRYDKFVTLSVREALRDAMAEEMR
RDDKVFLLGEEVAQYQGAYKISQGLLDEFGEKRVIDTPITEMGFAGLATGAAFSGLRPIVEFMTFNFSMQAIDQIINSAA
KTLYMSGGQMGCPIVFRGPNGAAARVGAQHSQCYASWYAHCPGLKVIAPWSAADAKGLLKAAIRDPNPVVFLENEILYGQ
TFEVPDDADFVLPIGKAKVERAGADVTLVAFSRMVGVALEAAKALAGEGIEAEVINLRTIRPLDVETILSSVRKTNRCVT
LEEGWPFAGIGAEIGMTIMENAFDYLDAPVIRITGEDVPMPYAANLEKLALPSIEAVVKAAKAACYKS

Sequences:

>Translated_468_residues
MPVQILMPALSPTMTEGTLAKWLKKEGDTIAAGDVIAEIETDKATMEFEAVDEGVLGQILIEAGTQNVPVNAPIGILLEE
GETIDDVHKPSASAPAPAKDVSLETTPAPAEPRRDPVPADTPLAAGGRAEAGRQEEKRYDKFVTLSVREALRDAMAEEMR
RDDKVFLLGEEVAQYQGAYKISQGLLDEFGEKRVIDTPITEMGFAGLATGAAFSGLRPIVEFMTFNFSMQAIDQIINSAA
KTLYMSGGQMGCPIVFRGPNGAAARVGAQHSQCYASWYAHCPGLKVIAPWSAADAKGLLKAAIRDPNPVVFLENEILYGQ
TFEVPDDADFVLPIGKAKVERAGADVTLVAFSRMVGVALEAAKALAGEGIEAEVINLRTIRPLDVETILSSVRKTNRCVT
LEEGWPFAGIGAEIGMTIMENAFDYLDAPVIRITGEDVPMPYAANLEKLALPSIEAVVKAAKAACYKS
>Mature_467_residues
PVQILMPALSPTMTEGTLAKWLKKEGDTIAAGDVIAEIETDKATMEFEAVDEGVLGQILIEAGTQNVPVNAPIGILLEEG
ETIDDVHKPSASAPAPAKDVSLETTPAPAEPRRDPVPADTPLAAGGRAEAGRQEEKRYDKFVTLSVREALRDAMAEEMRR
DDKVFLLGEEVAQYQGAYKISQGLLDEFGEKRVIDTPITEMGFAGLATGAAFSGLRPIVEFMTFNFSMQAIDQIINSAAK
TLYMSGGQMGCPIVFRGPNGAAARVGAQHSQCYASWYAHCPGLKVIAPWSAADAKGLLKAAIRDPNPVVFLENEILYGQT
FEVPDDADFVLPIGKAKVERAGADVTLVAFSRMVGVALEAAKALAGEGIEAEVINLRTIRPLDVETILSSVRKTNRCVTL
EEGWPFAGIGAEIGMTIMENAFDYLDAPVIRITGEDVPMPYAANLEKLALPSIEAVVKAAKAACYKS

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0022

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI156564403, Length=324, Percent_Identity=64.1975308641975, Blast_Score=439, Evalue=1e-123,
Organism=Homo sapiens, GI291084858, Length=324, Percent_Identity=60.4938271604938, Blast_Score=406, Evalue=1e-113,
Organism=Homo sapiens, GI4557353, Length=311, Percent_Identity=37.2990353697749, Blast_Score=203, Evalue=3e-52,
Organism=Homo sapiens, GI34101272, Length=311, Percent_Identity=37.2990353697749, Blast_Score=203, Evalue=3e-52,
Organism=Homo sapiens, GI203098753, Length=96, Percent_Identity=48.9583333333333, Blast_Score=106, Evalue=4e-23,
Organism=Homo sapiens, GI203098816, Length=96, Percent_Identity=48.9583333333333, Blast_Score=105, Evalue=7e-23,
Organism=Homo sapiens, GI31711992, Length=78, Percent_Identity=52.5641025641026, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI260898739, Length=59, Percent_Identity=57.6271186440678, Blast_Score=81, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17538422, Length=323, Percent_Identity=69.0402476780186, Blast_Score=460, Evalue=1e-130,
Organism=Caenorhabditis elegans, GI17506935, Length=323, Percent_Identity=38.6996904024768, Blast_Score=200, Evalue=1e-51,
Organism=Caenorhabditis elegans, GI17560088, Length=144, Percent_Identity=38.8888888888889, Blast_Score=91, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6319698, Length=330, Percent_Identity=62.7272727272727, Blast_Score=431, Evalue=1e-121,
Organism=Saccharomyces cerevisiae, GI6324258, Length=118, Percent_Identity=48.3050847457627, Blast_Score=99, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6321632, Length=98, Percent_Identity=37.7551020408163, Blast_Score=76, Evalue=1e-14,
Organism=Drosophila melanogaster, GI21358145, Length=320, Percent_Identity=65.9375, Blast_Score=436, Evalue=1e-122,
Organism=Drosophila melanogaster, GI24650940, Length=320, Percent_Identity=65.9375, Blast_Score=436, Evalue=1e-122,
Organism=Drosophila melanogaster, GI160714832, Length=318, Percent_Identity=36.4779874213836, Blast_Score=198, Evalue=6e-51,
Organism=Drosophila melanogaster, GI160714828, Length=318, Percent_Identity=36.4779874213836, Blast_Score=197, Evalue=1e-50,
Organism=Drosophila melanogaster, GI24650943, Length=90, Percent_Identity=68.8888888888889, Blast_Score=140, Evalue=2e-33,
Organism=Drosophila melanogaster, GI24650945, Length=90, Percent_Identity=68.8888888888889, Blast_Score=140, Evalue=2e-33,
Organism=Drosophila melanogaster, GI20129315, Length=76, Percent_Identity=51.3157894736842, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24582497, Length=65, Percent_Identity=50.7692307692308, Blast_Score=72, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR000089
- InterPro:   IPR011053
- InterPro:   IPR009014
- InterPro:   IPR015941
- InterPro:   IPR005475
- InterPro:   IPR005476 [H]

Pfam domain/function: PF00364 Biotin_lipoyl; PF02779 Transket_pyr; PF02780 Transketolase_C [H]

EC number: =1.2.4.1 [H]

Molecular weight: Translated: 50070; Mature: 49939

Theoretical pI: Translated: 4.40; Mature: 4.40

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVQILMPALSPTMTEGTLAKWLKKEGDTIAAGDVIAEIETDKATMEFEAVDEGVLGQIL
CCCEEEECCCCCCCCHHHHHHHHHHCCCEEEECCEEEEEECCCCEEEEHHHCCCHHHHHH
IEAGTQNVPVNAPIGILLEEGETIDDVHKPSASAPAPAKDVSLETTPAPAEPRRDPVPAD
HHCCCCCCCCCCCEEEEEECCCCHHHHCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCC
TPLAAGGRAEAGRQEEKRYDKFVTLSVREALRDAMAEEMRRDDKVFLLGEEVAQYQGAYK
CCCCCCCCCCCCCHHHHHHHHEEEHHHHHHHHHHHHHHHCCCCCEEEECHHHHHHCCHHH
ISQGLLDEFGEKRVIDTPITEMGFAGLATGAAFSGLRPIVEFMTFNFSMQAIDQIINSAA
HHHHHHHHHCCCCEECCCHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
KTLYMSGGQMGCPIVFRGPNGAAARVGAQHSQCYASWYAHCPGLKVIAPWSAADAKGLLK
HEEEECCCCCCCCEEEECCCCCHHHCCCHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHH
AAIRDPNPVVFLENEILYGQTFEVPDDADFVLPIGKAKVERAGADVTLVAFSRMVGVALE
HHHCCCCCEEEEECCEEECCEECCCCCCCEEEECCHHHHHHCCCCEEHHHHHHHHHHHHH
AAKALAGEGIEAEVINLRTIRPLDVETILSSVRKTNRCVTLEEGWPFAGIGAEIGMTIME
HHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHHHCCCEEEECCCCCCCCCCHHHHHHHHH
NAFDYLDAPVIRITGEDVPMPYAANLEKLALPSIEAVVKAAKAACYKS
HHHHHHCCCEEEECCCCCCCCHHCCHHHHCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PVQILMPALSPTMTEGTLAKWLKKEGDTIAAGDVIAEIETDKATMEFEAVDEGVLGQIL
CCEEEECCCCCCCCHHHHHHHHHHCCCEEEECCEEEEEECCCCEEEEHHHCCCHHHHHH
IEAGTQNVPVNAPIGILLEEGETIDDVHKPSASAPAPAKDVSLETTPAPAEPRRDPVPAD
HHCCCCCCCCCCCEEEEEECCCCHHHHCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCC
TPLAAGGRAEAGRQEEKRYDKFVTLSVREALRDAMAEEMRRDDKVFLLGEEVAQYQGAYK
CCCCCCCCCCCCCHHHHHHHHEEEHHHHHHHHHHHHHHHCCCCCEEEECHHHHHHCCHHH
ISQGLLDEFGEKRVIDTPITEMGFAGLATGAAFSGLRPIVEFMTFNFSMQAIDQIINSAA
HHHHHHHHHCCCCEECCCHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
KTLYMSGGQMGCPIVFRGPNGAAARVGAQHSQCYASWYAHCPGLKVIAPWSAADAKGLLK
HEEEECCCCCCCCEEEECCCCCHHHCCCHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHH
AAIRDPNPVVFLENEILYGQTFEVPDDADFVLPIGKAKVERAGADVTLVAFSRMVGVALE
HHHCCCCCEEEEECCEEECCEECCCCCCCEEEECCHHHHHHCCCCEEHHHHHHHHHHHHH
AAKALAGEGIEAEVINLRTIRPLDVETILSSVRKTNRCVTLEEGWPFAGIGAEIGMTIME
HHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHHHCCCEEEECCCCCCCCCCHHHHHHHHH
NAFDYLDAPVIRITGEDVPMPYAANLEKLALPSIEAVVKAAKAACYKS
HHHHHHCCCEEEECCCCCCCCHHCCHHHHCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10796014; 11481430 [H]