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The map label for this gene is menB [C]

Identifier: 83593169

GI number: 83593169

Start: 2130827

End: 2131903

Strand: Reverse

Name: menB [C]

Synonym: Rru_A1834

Alternate gene names: 83593169

Gene position: 2131903-2130827 (Counterclockwise)

Preceding gene: 83593170

Following gene: 83593168

Centisome position: 48.98

GC content: 68.8

Gene sequence:

>1077_bases
ATGAGCGACGACGACATCCTGTTTGAGCAAAAGGGCGCCTTGGGCGCCATCACCCTTAACCGGCCGCGCGCCCTGAACGC
CCTGACCCTGCCCAAGGTCGAGCGGATGGACCGCGCCCTGCGCGCCTGGGCCACCGATAAGACGGTGGGCGTGGTCACGG
TCGAAGGGTCGGGCGGCCGGGCGTTTTGCGCCGGTGGCGATGTCCGCGCCCTGGCTGAGGTCGGCAAAAATGGCGCGAGC
GCCTTTCTGGATGCCTTCTTCGGCCAGGAATATCGCCTCAACCGCCTGATCAAGACCTATCCCAAGCCCTATGTCGCCCT
GCTCGGCGGCATCACCATGGGCGGCGGGGTGGGCATTTCGATCCATGGCCGCTTTCCTGTGGCCACCGAAGCCACCCTCA
TCGCCATGCCCGAAACCGGCATCGGCATGTTCCCCGATGTCGGCGGCACCTATTTTCTGCCGCGCTGCCCGGGGGCGACG
GGGGTTTATCTGGCCCTGACCGGAGCGCGGATCGGCGCGGCCGACGCGGTCGCTTTGGGGTTGTGCACCCACCATATCCC
CTTAAGCGCCCTGGAGGCGGTCAAGGAGGCCCTGGCGGCTTTGGACGCGGACGCCGGAGCCGACGGGGTGAAGGCGGTGC
TTGACGGCTTCCACCGCGATCCGGGCGAGGCGCCGCTGGCACCCAGGCGCGCCGCCATCGCCCGCTGTTTCGGCCAGCCC
ACCCTCGAGGACATCCTGGCCGCCCTGGCCAGCGAGGCGGCGATCGGCGATCGGGCCTGGGCGGAGGAAACCCGGGCCGG
CCTGTTGGCGAAATCGCCCTTCGCCACGCGGGTGACCTTCGAGCAATTGCGCCGGGGCGCCACCCTCGATTTCGACGGCT
GCATGGCCTTGGAATACCGCATGGCGCCGCGCATCGCCCGCCATCCCGATTTCATCGAAGGCGTGCGCGCCCTGCTTGTC
GATAAGGACAACCAGCCGCGGTGGTCGGCCAAGGCCCTCGCCGAGGTCGAGGACGCCGCCGTCGGAGCGCTGTTCGCGCC
GCGTGCCGATGGCGATCTGGAGTTTCGCGACCCTTAA

Upstream 100 bases:

>100_bases
GGATCTTGGAAGGCACCAACGAGATCATGCGGGTGATCATCGGCCGCCGACTGCTGGCCGATTAGCAGATCCCCCGCTTC
CCCTGCGTCAAGGACCCGTG

Downstream 100 bases:

>100_bases
AAAACCTTAAGCATGGGAGGAAGATATGGCCAAGATCGCTTTCATCGGCCTGGGCAATATGGGCGGACCGATGCTGCGCA
ACCTGCTCAAGGCCGGCCAC

Product: enoyl-CoA hydratase/isomerase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 358; Mature: 357

Protein sequence:

>358_residues
MSDDDILFEQKGALGAITLNRPRALNALTLPKVERMDRALRAWATDKTVGVVTVEGSGGRAFCAGGDVRALAEVGKNGAS
AFLDAFFGQEYRLNRLIKTYPKPYVALLGGITMGGGVGISIHGRFPVATEATLIAMPETGIGMFPDVGGTYFLPRCPGAT
GVYLALTGARIGAADAVALGLCTHHIPLSALEAVKEALAALDADAGADGVKAVLDGFHRDPGEAPLAPRRAAIARCFGQP
TLEDILAALASEAAIGDRAWAEETRAGLLAKSPFATRVTFEQLRRGATLDFDGCMALEYRMAPRIARHPDFIEGVRALLV
DKDNQPRWSAKALAEVEDAAVGALFAPRADGDLEFRDP

Sequences:

>Translated_358_residues
MSDDDILFEQKGALGAITLNRPRALNALTLPKVERMDRALRAWATDKTVGVVTVEGSGGRAFCAGGDVRALAEVGKNGAS
AFLDAFFGQEYRLNRLIKTYPKPYVALLGGITMGGGVGISIHGRFPVATEATLIAMPETGIGMFPDVGGTYFLPRCPGAT
GVYLALTGARIGAADAVALGLCTHHIPLSALEAVKEALAALDADAGADGVKAVLDGFHRDPGEAPLAPRRAAIARCFGQP
TLEDILAALASEAAIGDRAWAEETRAGLLAKSPFATRVTFEQLRRGATLDFDGCMALEYRMAPRIARHPDFIEGVRALLV
DKDNQPRWSAKALAEVEDAAVGALFAPRADGDLEFRDP
>Mature_357_residues
SDDDILFEQKGALGAITLNRPRALNALTLPKVERMDRALRAWATDKTVGVVTVEGSGGRAFCAGGDVRALAEVGKNGASA
FLDAFFGQEYRLNRLIKTYPKPYVALLGGITMGGGVGISIHGRFPVATEATLIAMPETGIGMFPDVGGTYFLPRCPGATG
VYLALTGARIGAADAVALGLCTHHIPLSALEAVKEALAALDADAGADGVKAVLDGFHRDPGEAPLAPRRAAIARCFGQPT
LEDILAALASEAAIGDRAWAEETRAGLLAKSPFATRVTFEQLRRGATLDFDGCMALEYRMAPRIARHPDFIEGVRALLVD
KDNQPRWSAKALAEVEDAAVGALFAPRADGDLEFRDP

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI37594471, Length=358, Percent_Identity=39.9441340782123, Blast_Score=273, Evalue=2e-73,
Organism=Homo sapiens, GI37594469, Length=304, Percent_Identity=39.4736842105263, Blast_Score=233, Evalue=2e-61,
Organism=Homo sapiens, GI194097323, Length=197, Percent_Identity=32.48730964467, Blast_Score=93, Evalue=4e-19,
Organism=Escherichia coli, GI1788597, Length=191, Percent_Identity=27.2251308900524, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI221142681, Length=262, Percent_Identity=27.4809160305344, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1787659, Length=188, Percent_Identity=30.8510638297872, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1788682, Length=182, Percent_Identity=26.9230769230769, Blast_Score=62, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI25144160, Length=350, Percent_Identity=42, Blast_Score=271, Evalue=4e-73,
Organism=Caenorhabditis elegans, GI25144157, Length=350, Percent_Identity=42, Blast_Score=271, Evalue=5e-73,
Organism=Caenorhabditis elegans, GI25145438, Length=176, Percent_Identity=30.6818181818182, Blast_Score=82, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI17554946, Length=189, Percent_Identity=31.2169312169312, Blast_Score=81, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI17558304, Length=179, Percent_Identity=31.8435754189944, Blast_Score=75, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17549921, Length=210, Percent_Identity=27.6190476190476, Blast_Score=71, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17540714, Length=177, Percent_Identity=24.8587570621469, Blast_Score=65, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI25144276, Length=164, Percent_Identity=29.2682926829268, Blast_Score=65, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI17508953, Length=164, Percent_Identity=29.2682926829268, Blast_Score=65, Evalue=8e-11,
Organism=Caenorhabditis elegans, GI17508951, Length=164, Percent_Identity=29.2682926829268, Blast_Score=64, Evalue=1e-10,
Organism=Saccharomyces cerevisiae, GI6320241, Length=354, Percent_Identity=33.8983050847458, Blast_Score=184, Evalue=2e-47,
Organism=Drosophila melanogaster, GI28571730, Length=353, Percent_Identity=42.2096317280453, Blast_Score=273, Evalue=2e-73,
Organism=Drosophila melanogaster, GI28571729, Length=353, Percent_Identity=42.2096317280453, Blast_Score=272, Evalue=2e-73,
Organism=Drosophila melanogaster, GI20129971, Length=185, Percent_Identity=33.5135135135135, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24653477, Length=185, Percent_Identity=33.5135135135135, Blast_Score=91, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 37756; Mature: 37624

Theoretical pI: Translated: 5.48; Mature: 5.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDDDILFEQKGALGAITLNRPRALNALTLPKVERMDRALRAWATDKTVGVVTVEGSGGR
CCCCCEEEECCCCEEEEEECCCCCCCEECCHHHHHHHHHHHHHCCCCEEEEEEEECCCCE
AFCAGGDVRALAEVGKNGASAFLDAFFGQEYRLNRLIKTYPKPYVALLGGITMGGGVGIS
EEECCCCHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHCCCEECCCEEEE
IHGRFPVATEATLIAMPETGIGMFPDVGGTYFLPRCPGATGVYLALTGARIGAADAVALG
EECCCCCCCCEEEEEECCCCCCCCCCCCCCEEECCCCCCCEEEEEEECCCCCCHHHHHHH
LCTHHIPLSALEAVKEALAALDADAGADGVKAVLDGFHRDPGEAPLAPRRAAIARCFGQP
HHHHCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCC
TLEDILAALASEAAIGDRAWAEETRAGLLAKSPFATRVTFEQLRRGATLDFDGCMALEYR
CHHHHHHHHHHHHHCCCHHHHHHHHCCCEECCCCHHHHHHHHHHCCCCCCCCCCEEEEEH
MAPRIARHPDFIEGVRALLVDKDNQPRWSAKALAEVEDAAVGALFAPRADGDLEFRDP
HCCHHHCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHEECCCCCCCCCCCCC
>Mature Secondary Structure 
SDDDILFEQKGALGAITLNRPRALNALTLPKVERMDRALRAWATDKTVGVVTVEGSGGR
CCCCEEEECCCCEEEEEECCCCCCCEECCHHHHHHHHHHHHHCCCCEEEEEEEECCCCE
AFCAGGDVRALAEVGKNGASAFLDAFFGQEYRLNRLIKTYPKPYVALLGGITMGGGVGIS
EEECCCCHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHCCCEECCCEEEE
IHGRFPVATEATLIAMPETGIGMFPDVGGTYFLPRCPGATGVYLALTGARIGAADAVALG
EECCCCCCCCEEEEEECCCCCCCCCCCCCCEEECCCCCCCEEEEEEECCCCCCHHHHHHH
LCTHHIPLSALEAVKEALAALDADAGADGVKAVLDGFHRDPGEAPLAPRRAAIARCFGQP
HHHHCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCC
TLEDILAALASEAAIGDRAWAEETRAGLLAKSPFATRVTFEQLRRGATLDFDGCMALEYR
CHHHHHHHHHHHHHCCCHHHHHHHHCCCEECCCCHHHHHHHHHHCCCCCCCCCCEEEEEH
MAPRIARHPDFIEGVRALLVDKDNQPRWSAKALAEVEDAAVGALFAPRADGDLEFRDP
HCCHHHCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA