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The map label for this gene is glyA

Identifier: 83593162

GI number: 83593162

Start: 2125210

End: 2126502

Strand: Reverse

Name: glyA

Synonym: Rru_A1827

Alternate gene names: 83593162

Gene position: 2126502-2125210 (Counterclockwise)

Preceding gene: 83593163

Following gene: 83593161

Centisome position: 48.85

GC content: 65.51

Gene sequence:

>1293_bases
ATGACCGCCTACACCCCTTGGACCGGATTCTTTTCCGCCTCCGTCGCCCAAGCCGATCCCGAGCTTGACCGGGTGCTGCG
GGCCGAGTTGTCCCGCCAGCAGGACCAGATCGAGCTGATCGCCTCGGAAAACATCGTGTCCCGCGCCGTCCTCGAGGCCG
CGGGCAGCGTTTTGACCAATAAATACGCCGAAGGCTATCCCGGCAAGCGCTACTACGGCGGCTGCGAAGAGGTCGACGTG
GCCGAGGAGTTGGCCATCGAGCGGGCCAAGGCGTTGTTCGGCTGCTCCTACGTTAACGTCCAGCCCCATTCCGGCGCCCA
GGCCAATGGCGCGGTGATGATGGCCCTGGTCAAGCCCGGCGACACCATCATGGGCATGAGCCTGGCCGCCGGCGGTCACC
TGACCCATGGCGCGCCGCCGGCCCAATCGGGCAAGTGGTTCAACGCCGTGCAGTACGGCGTCCGCCTGCAAGACGCCAGC
ATCGATTTCGACGAGGTGGCGACCCTGGCCGAAACCCACAAGCCCAAGCTGATCATCGCCGGCGGCTCGGCCTATCCCCG
CATCATCGATTTCGCCAAGTTCCGCGAGATCGCCGATCGGGTCGGCGCGCTGTTCATGGTCGATATGGCCCATTTCGCCG
GTCTGGTGGCCGCCGGGCTCCACCCCAGCCCGCTGCCCTATGCCGATATCGTCACCACCACCACCCATAAAACCCTGCGC
GGCCCGCGCGGCGGCATGGTGTTGTCGAACAATCCCGACATCGGCAAGAAGATCAATTCGGCGGTCTTCCCCGGGCTGCA
GGGCGGCCCCTTGATGCATATCATCGCCGCCAAGGCCGTGGCCTTCGGCGAGGCGCTGCGCCCCGAGTTCAAGGTCTATG
CCCAGGCGGTGATCGATAACGCCAAGGCGCTGACCGACGCCCTCGCCGCCGGCGGGCTGAACATCGTGTCGGGCGGCACC
GATACCCACCTCGCCCTGGTCGATCTGCGGCCCAAGGCTTTGACCGGCAATATCGTCGAGAAGAGCCTGGAGCGGGCCAA
TATCACCACCAACAAGAACGGCATTCCCTTCGACCCGGAAAAGCCGGCCATCACCTCGGGCATCCGCGTCGGCACCCCGG
CGGGCACCACCCGGGGCTTTGGCACCGCCGAGTTCACCGAGATCGGCAAGCTGATCGTCGAAGTGCTCGACGGGCTGGCG
GCCAATGGCGAGGACAATTCCCAGGCCGAGGCCGCCGTGCGCGAAAAGGTCGCCGTCCTTTGCCGTCGCTTTCCCATATA
TGGTGGGTTATAA

Upstream 100 bases:

>100_bases
CATGCGCGCCGGGTGGCCAAGCTGGGGACCCCCTCGGCCTGATCAAGACCCGGTTTTTTCGCTTTCTTTCTCTTTGACCC
GCGAACGGAAGACCAGACCC

Downstream 100 bases:

>100_bases
GTCTTCAAGGAACACATCTTGAAGACTGGGGAGGCTCCCCTCGATGCGCTGCCCTTTCTGCGGCAACGGTGATACGCAGG
TAAAAGATTCGCGCCCCACG

Product: serine hydroxymethyltransferase

Products: NA

Alternate protein names: SHMT 2; Serine methylase 2

Number of amino acids: Translated: 430; Mature: 429

Protein sequence:

>430_residues
MTAYTPWTGFFSASVAQADPELDRVLRAELSRQQDQIELIASENIVSRAVLEAAGSVLTNKYAEGYPGKRYYGGCEEVDV
AEELAIERAKALFGCSYVNVQPHSGAQANGAVMMALVKPGDTIMGMSLAAGGHLTHGAPPAQSGKWFNAVQYGVRLQDAS
IDFDEVATLAETHKPKLIIAGGSAYPRIIDFAKFREIADRVGALFMVDMAHFAGLVAAGLHPSPLPYADIVTTTTHKTLR
GPRGGMVLSNNPDIGKKINSAVFPGLQGGPLMHIIAAKAVAFGEALRPEFKVYAQAVIDNAKALTDALAAGGLNIVSGGT
DTHLALVDLRPKALTGNIVEKSLERANITTNKNGIPFDPEKPAITSGIRVGTPAGTTRGFGTAEFTEIGKLIVEVLDGLA
ANGEDNSQAEAAVREKVAVLCRRFPIYGGL

Sequences:

>Translated_430_residues
MTAYTPWTGFFSASVAQADPELDRVLRAELSRQQDQIELIASENIVSRAVLEAAGSVLTNKYAEGYPGKRYYGGCEEVDV
AEELAIERAKALFGCSYVNVQPHSGAQANGAVMMALVKPGDTIMGMSLAAGGHLTHGAPPAQSGKWFNAVQYGVRLQDAS
IDFDEVATLAETHKPKLIIAGGSAYPRIIDFAKFREIADRVGALFMVDMAHFAGLVAAGLHPSPLPYADIVTTTTHKTLR
GPRGGMVLSNNPDIGKKINSAVFPGLQGGPLMHIIAAKAVAFGEALRPEFKVYAQAVIDNAKALTDALAAGGLNIVSGGT
DTHLALVDLRPKALTGNIVEKSLERANITTNKNGIPFDPEKPAITSGIRVGTPAGTTRGFGTAEFTEIGKLIVEVLDGLA
ANGEDNSQAEAAVREKVAVLCRRFPIYGGL
>Mature_429_residues
TAYTPWTGFFSASVAQADPELDRVLRAELSRQQDQIELIASENIVSRAVLEAAGSVLTNKYAEGYPGKRYYGGCEEVDVA
EELAIERAKALFGCSYVNVQPHSGAQANGAVMMALVKPGDTIMGMSLAAGGHLTHGAPPAQSGKWFNAVQYGVRLQDASI
DFDEVATLAETHKPKLIIAGGSAYPRIIDFAKFREIADRVGALFMVDMAHFAGLVAAGLHPSPLPYADIVTTTTHKTLRG
PRGGMVLSNNPDIGKKINSAVFPGLQGGPLMHIIAAKAVAFGEALRPEFKVYAQAVIDNAKALTDALAAGGLNIVSGGTD
THLALVDLRPKALTGNIVEKSLERANITTNKNGIPFDPEKPAITSGIRVGTPAGTTRGFGTAEFTEIGKLIVEVLDGLAA
NGEDNSQAEAAVREKVAVLCRRFPIYGGL

Specific function: Interconversion of serine and glycine

COG id: COG0112

COG function: function code E; Glycine/serine hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SHMT family

Homologues:

Organism=Homo sapiens, GI19923315, Length=462, Percent_Identity=45.6709956709957, Blast_Score=358, Evalue=4e-99,
Organism=Homo sapiens, GI261862352, Length=462, Percent_Identity=45.6709956709957, Blast_Score=358, Evalue=5e-99,
Organism=Homo sapiens, GI261862350, Length=462, Percent_Identity=45.6709956709957, Blast_Score=358, Evalue=5e-99,
Organism=Homo sapiens, GI261862348, Length=462, Percent_Identity=45.6709956709957, Blast_Score=358, Evalue=5e-99,
Organism=Homo sapiens, GI261862346, Length=463, Percent_Identity=45.7883369330454, Blast_Score=348, Evalue=6e-96,
Organism=Homo sapiens, GI22547186, Length=456, Percent_Identity=44.5175438596491, Blast_Score=320, Evalue=1e-87,
Organism=Homo sapiens, GI22547189, Length=442, Percent_Identity=41.4027149321267, Blast_Score=281, Evalue=7e-76,
Organism=Escherichia coli, GI1788902, Length=418, Percent_Identity=56.9377990430622, Blast_Score=448, Evalue=1e-127,
Organism=Caenorhabditis elegans, GI25144732, Length=415, Percent_Identity=46.2650602409639, Blast_Score=336, Evalue=1e-92,
Organism=Caenorhabditis elegans, GI25144729, Length=415, Percent_Identity=46.2650602409639, Blast_Score=335, Evalue=2e-92,
Organism=Saccharomyces cerevisiae, GI6319739, Length=404, Percent_Identity=46.7821782178218, Blast_Score=352, Evalue=5e-98,
Organism=Saccharomyces cerevisiae, GI6323087, Length=406, Percent_Identity=45.8128078817734, Blast_Score=343, Evalue=4e-95,
Organism=Drosophila melanogaster, GI24640005, Length=461, Percent_Identity=45.3362255965293, Blast_Score=365, Evalue=1e-101,
Organism=Drosophila melanogaster, GI221329721, Length=461, Percent_Identity=45.3362255965293, Blast_Score=364, Evalue=1e-101,

Paralogues:

None

Copy number: 3180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 12,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): GLYA2_RHORT (Q2RTB8)

Other databases:

- EMBL:   CP000230
- RefSeq:   YP_426914.1
- ProteinModelPortal:   Q2RTB8
- SMR:   Q2RTB8
- STRING:   Q2RTB8
- GeneID:   3835251
- GenomeReviews:   CP000230_GR
- KEGG:   rru:Rru_A1827
- NMPDR:   fig|1085.1.peg.2402
- eggNOG:   COG0112
- HOGENOM:   HBG301263
- OMA:   DYDQIAA
- PhylomeDB:   Q2RTB8
- ProtClustDB:   PRK00011
- BioCyc:   RRUB269796:RRU_A1827-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00051_B
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR001085
- InterPro:   IPR019798
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PANTHER:   PTHR11680
- PIRSF:   PIRSF000412

Pfam domain/function: PF00464 SHMT; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.1.2.1

Molecular weight: Translated: 45352; Mature: 45221

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: PS00096 SHMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAYTPWTGFFSASVAQADPELDRVLRAELSRQQDQIELIASENIVSRAVLEAAGSVLTN
CCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
KYAEGYPGKRYYGGCEEVDVAEELAIERAKALFGCSYVNVQPHSGAQANGAVMMALVKPG
HHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCEEEEEEECCC
DTIMGMSLAAGGHLTHGAPPAQSGKWFNAVQYGVRLQDASIDFDEVATLAETHKPKLIIA
CCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHCEEEECCCCCHHHHHHHHHCCCCEEEEE
GGSAYPRIIDFAKFREIADRVGALFMVDMAHFAGLVAAGLHPSPLPYADIVTTTTHKTLR
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHEECCCHHHHC
GPRGGMVLSNNPDIGKKINSAVFPGLQGGPLMHIIAAKAVAFGEALRPEFKVYAQAVIDN
CCCCCEEECCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHH
AKALTDALAAGGLNIVSGGTDTHLALVDLRPKALTGNIVEKSLERANITTNKNGIPFDPE
HHHHHHHHHHCCCEEECCCCCCEEEEEECCCCHHCCHHHHHHHHHCCCCCCCCCCCCCCC
KPAITSGIRVGTPAGTTRGFGTAEFTEIGKLIVEVLDGLAANGEDNSQAEAAVREKVAVL
CCCHHCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
CRRFPIYGGL
HHHCCCCCCC
>Mature Secondary Structure 
TAYTPWTGFFSASVAQADPELDRVLRAELSRQQDQIELIASENIVSRAVLEAAGSVLTN
CCCCCCCCHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
KYAEGYPGKRYYGGCEEVDVAEELAIERAKALFGCSYVNVQPHSGAQANGAVMMALVKPG
HHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCEEEEEEECCC
DTIMGMSLAAGGHLTHGAPPAQSGKWFNAVQYGVRLQDASIDFDEVATLAETHKPKLIIA
CCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHCEEEECCCCCHHHHHHHHHCCCCEEEEE
GGSAYPRIIDFAKFREIADRVGALFMVDMAHFAGLVAAGLHPSPLPYADIVTTTTHKTLR
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHEECCCHHHHC
GPRGGMVLSNNPDIGKKINSAVFPGLQGGPLMHIIAAKAVAFGEALRPEFKVYAQAVIDN
CCCCCEEECCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHH
AKALTDALAAGGLNIVSGGTDTHLALVDLRPKALTGNIVEKSLERANITTNKNGIPFDPE
HHHHHHHHHHCCCEEECCCCCCEEEEEECCCCHHCCHHHHHHHHHCCCCCCCCCCCCCCC
KPAITSGIRVGTPAGTTRGFGTAEFTEIGKLIVEVLDGLAANGEDNSQAEAAVREKVAVL
CCCHHCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
CRRFPIYGGL
HHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA