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The map label for this gene is pcm

Identifier: 83593103

GI number: 83593103

Start: 2060816

End: 2061475

Strand: Reverse

Name: pcm

Synonym: Rru_A1768

Alternate gene names: 83593103

Gene position: 2061475-2060816 (Counterclockwise)

Preceding gene: 83593104

Following gene: 83593102

Centisome position: 47.36

GC content: 68.48

Gene sequence:

>660_bases
GTGAGCGTTCCATCGCGCAAGATCCGGCTGATCATGGAGTTGCGCCAGAACGGCGTATCGGCCACTCCCGTTCTCGCCGC
CATCGAGCGGGTGCCACGCGACGCCTTCGTTTCGGCGCCCTTCTCCGATCAGGCCTATGAGAACACCGCCCTGCCCATCG
GCTGCGGCCAAACGATCAGCCAACCGCTGGTGGTCGGCTTGATGACCCAGGCCCTGGATTTGAACGACCGCCACAAGGTG
CTGGAAATCGGCACCGGATCGGGATACCAGACCGCCGTTCTCGCCCGCTTGTGCCGGCGGGTTTATACGATCGAACGCCA
TGGCGCGCTGCTGCGCGAGGCCGAGGCCCGGCTGACCGCCCTGGGCCTGCATCGCACCGTGGTCACCCGCGAGGGCGATG
GCGGACGCGGCTGGCCCGAACAAGCCCCCTTTGAACGCATCCTGGTCACCGCCGCCGCCCTGGATATCCCCAAGGTGCTG
GTCGCCCAGTTGGCCATCGGCGGGGTGATGGTTTTGCCCGTGGGCAAGGAGAGCGGCGCCCAGGAGGTGGTTCGCGTGCG
CCGCACCGCCGAGGACGCCCTGGTGACCGAACGCCTGTTCCCCGTGCGCTTCGTTCCGCTGGTCGATGGCCTGCCGCCGC
GCGACGCGCCCGGGGCATGA

Upstream 100 bases:

>100_bases
GCCATCACCGTGACCCCGCTGTGTGTCGATATGACCGATCTGCCGACGATCGAGGCGTTGACGGGGGCCTTTTGACATCC
AATCCAGGGAGAGCGGCCCC

Downstream 100 bases:

>100_bases
GCGCCCCCTTACCACCTCTGATGCCCTGGCGCCCCATTGTGGGGGCGGACCAGCACCCCTATAGTGGGGCCATGACCTCG
TCCGCGCAGAATATATCCCG

Product: protein-L-isoaspartate(D-aspartate) O-methyltransferase

Products: NA

Alternate protein names: L-isoaspartyl protein carboxyl methyltransferase; Protein L-isoaspartyl methyltransferase; Protein-beta-aspartate methyltransferase; PIMT

Number of amino acids: Translated: 219; Mature: 218

Protein sequence:

>219_residues
MSVPSRKIRLIMELRQNGVSATPVLAAIERVPRDAFVSAPFSDQAYENTALPIGCGQTISQPLVVGLMTQALDLNDRHKV
LEIGTGSGYQTAVLARLCRRVYTIERHGALLREAEARLTALGLHRTVVTREGDGGRGWPEQAPFERILVTAAALDIPKVL
VAQLAIGGVMVLPVGKESGAQEVVRVRRTAEDALVTERLFPVRFVPLVDGLPPRDAPGA

Sequences:

>Translated_219_residues
MSVPSRKIRLIMELRQNGVSATPVLAAIERVPRDAFVSAPFSDQAYENTALPIGCGQTISQPLVVGLMTQALDLNDRHKV
LEIGTGSGYQTAVLARLCRRVYTIERHGALLREAEARLTALGLHRTVVTREGDGGRGWPEQAPFERILVTAAALDIPKVL
VAQLAIGGVMVLPVGKESGAQEVVRVRRTAEDALVTERLFPVRFVPLVDGLPPRDAPGA
>Mature_218_residues
SVPSRKIRLIMELRQNGVSATPVLAAIERVPRDAFVSAPFSDQAYENTALPIGCGQTISQPLVVGLMTQALDLNDRHKVL
EIGTGSGYQTAVLARLCRRVYTIERHGALLREAEARLTALGLHRTVVTREGDGGRGWPEQAPFERILVTAAALDIPKVLV
AQLAIGGVMVLPVGKESGAQEVVRVRRTAEDALVTERLFPVRFVPLVDGLPPRDAPGA

Specific function: Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins

COG id: COG2518

COG function: function code O; Protein-L-isoaspartate carboxylmethyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. L- isoaspartyl/D-aspartyl protein methyltransferase family

Homologues:

Organism=Homo sapiens, GI226530908, Length=214, Percent_Identity=31.3084112149533, Blast_Score=77, Evalue=1e-14,
Organism=Escherichia coli, GI1789100, Length=207, Percent_Identity=49.2753623188406, Blast_Score=179, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI71983477, Length=223, Percent_Identity=33.6322869955157, Blast_Score=77, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI193207222, Length=221, Percent_Identity=32.1266968325792, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI17981723, Length=229, Percent_Identity=31.8777292576419, Blast_Score=101, Evalue=3e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PIMT_RHORT (Q2RTH7)

Other databases:

- EMBL:   CP000230
- RefSeq:   YP_426855.1
- ProteinModelPortal:   Q2RTH7
- SMR:   Q2RTH7
- STRING:   Q2RTH7
- GeneID:   3835190
- GenomeReviews:   CP000230_GR
- KEGG:   rru:Rru_A1768
- NMPDR:   fig|1085.1.peg.2343
- eggNOG:   COG2518
- HOGENOM:   HBG699907
- OMA:   STIRNDG
- PhylomeDB:   Q2RTH7
- BioCyc:   RRUB269796:RRU_A1768-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00090
- InterPro:   IPR000682
- PANTHER:   PTHR11579
- TIGRFAMs:   TIGR00080

Pfam domain/function: PF01135 PCMT

EC number: =2.1.1.77

Molecular weight: Translated: 23628; Mature: 23497

Theoretical pI: Translated: 9.25; Mature: 9.25

Prosite motif: PS01279 PCMT

Important sites: ACT_SITE 60-60

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVPSRKIRLIMELRQNGVSATPVLAAIERVPRDAFVSAPFSDQAYENTALPIGCGQTIS
CCCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHEEECCCCCCCCCCCCCCCCCCCHHH
QPLVVGLMTQALDLNDRHKVLEIGTGSGYQTAVLARLCRRVYTIERHGALLREAEARLTA
HHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGLHRTVVTREGDGGRGWPEQAPFERILVTAAALDIPKVLVAQLAIGGVMVLPVGKESGA
HHHHHEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCH
QEVVRVRRTAEDALVTERLFPVRFVPLVDGLPPRDAPGA
HHHHHHHHHHHHHHHHHHHCCEEEEHHHCCCCCCCCCCC
>Mature Secondary Structure 
SVPSRKIRLIMELRQNGVSATPVLAAIERVPRDAFVSAPFSDQAYENTALPIGCGQTIS
CCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHEEECCCCCCCCCCCCCCCCCCCHHH
QPLVVGLMTQALDLNDRHKVLEIGTGSGYQTAVLARLCRRVYTIERHGALLREAEARLTA
HHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGLHRTVVTREGDGGRGWPEQAPFERILVTAAALDIPKVLVAQLAIGGVMVLPVGKESGA
HHHHHEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCH
QEVVRVRRTAEDALVTERLFPVRFVPLVDGLPPRDAPGA
HHHHHHHHHHHHHHHHHHHCCEEEEHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA