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The map label for this gene is recG [H]

Identifier: 83593063

GI number: 83593063

Start: 2022753

End: 2024864

Strand: Direct

Name: recG [H]

Synonym: Rru_A1728

Alternate gene names: 83593063

Gene position: 2022753-2024864 (Clockwise)

Preceding gene: 83593059

Following gene: 83593065

Centisome position: 46.47

GC content: 70.12

Gene sequence:

>2112_bases
ATGCGTCCCAGCCTTCTTGATCCTTTGTTCCGGCCGCTGACCTCGCTCAAGGGCGCGGGCAAAACCATGGCGCCGCTGAT
CGCCCGGCTGATCGGCGGCGACAAGGTGGTCGATCTGTTGTGGCATCTGCCCAGCGGTCTGGTCGATCGCCGGTTCTCGC
CGTTGATCGCCGAGGCCCCCGATGGCGTCGTCGTTACCCTGACGGTGGTGGTCGAGGCCCATCAGGAACCGCCGCCGCGC
TCGCCCTCGCCCTATCGGGTGGTCTGCCGCGACGCCAGCGGCTTCGTGACCCTGGTATTCTTCCATGGCCGGGCGCGCTA
TCTGAACGATTTGCTACCGGTCGGCGAAACCCGGGTGATCAGCGGCAAGGTCGAACGCTTCGGCGGCGCGCCGCAGATTG
TCCATCCCACCCATGTCGTGCCCTTGGCCGAGGCCGAGGCGGTGTGCCGGGTTGAACCGGTCTATCCCTTGAGTGGCGGC
GTGGCCGGCAAGGTTCTGGCCCGGTTGATCGCCCAGGCCCTTGATGACATCCCCGATCCCGCCGCCTGGCCCGATGAATG
GATCGACGCCCCCCTGAAAGCCCGCGAAGGCTGGCCGGGCTGGATCGACGCCCTGCGCGCCGTTCACGCCCCCGAGGAGG
CCGAAGACCTGCGCCCCGACCACCCGGCGCGGCGGCGGCTGGCCTATGACGAATTGCTGGCCACCCAACTCGCCCTGTTG
CTGGTGCGGCGGGCGGCGCGCACGGTGCGCGGTCGGCCGATCGTCGGCACCGGCGCCCTGCGCGCCAAGGTGCTGGCGGC
GCTGCCCTTCGCCCTGACCGGGGCGCAAAGCCGCTCGCTGGCCGAGATCGACGGCGACATGGCCTCGCCGGCCCGCATGT
TGCGCCTGCTGCAGGGCGATGTCGGCAGCGGCAAGACGGTGGTGGCGCTGCTGACCATGCTGACGGCGGTGGAGGCTGGC
GCCCAGGCCGCCCTGATGGCGCCGACCGAAATCCTCGCCCGCCAGCACATCGAAACCCTGGCGCCGCTCTGCGCCAGCGC
CGGGGTGCGCCTGGGCTTGCTGACCGGGCGCGACAAGGGGCGGGCGCGCGCCGCCCTGCTCGAGGCCCTGGCGGCGGGCG
AGATCGATATCCTGGTCGGCACCCACGCCCTGTTCCAAGACGACGTGGTCTTCGCCGCCCTCGCCGTGGTGGTGGTCGAT
GAACAGCATCGCTTTGGCGTTCACCAGCGTCTGGCGCTGTCGGACAAGGGCCGGGCGGTCGACGTGTTGGTGATGACCGC
CACGCCGATCCCGCGCACCCTGACGCTCACCCATTACGGCGACATGGATATCTCGCGGCTTGATGAAAAGCCGCCGGGGC
GGCTTCCCGCCGATACCCGGGTGCTGCCGATCGACCGCCTGGACGACGTGATCGCCGCCGTCGCCCGCGCCATCGACGGC
GGGGCCAAGGTCTATTGGGTCTGCCCGCTGATCGAGGACTCGGAAACCGGCGACATGGCCGCCGCCGTCGATCGCCAAGC
CCTGCTTGCCGACAGGCTGGGGCCACGCCTCGGCCCCCGGGTCGGCCTTGTCCACGGCCGGATGAAGCCCGGCGAAAAAG
ACGCGGTGATGGAGGCCTTTTCCGGGAACGGGCTTGATCTGTTGGTGGCGACGACGGTGATCGAGGTCGGCGTCAATGTG
CCAAGCGCCACGGTGATGGTGATCGAACACGCCGAGCGCTTCGGCCTTGCCCAGCTACACCAGTTGCGCGGCCGCATCGG
CCGGGGCGGCGGGCGTTCGACCTGCCTGTTGCTCTATGCCCCGCCCTTGGGGGAAACCGCCCGCGCCCGCCTGGAGACCA
TGCGCCGCACCGACGACGGCTTCGAGATCGCCGAAGAGGATCTGCGGCTGCGCGGCGCCGGCGAGGTTCTGGGCACCCGC
CAAAGCGGCCTGCCGGTCTTCCGGCTGATCGATCCGCTGCTCGCCGAAGACCTGCTCGCCATCGCCCGCAAGCAGGCCGA
GGTGATCGTCGAGACCGACCCCGATCTGGCCGGCCCCCATGGCGCGGCGCTGCGCGTTCTGCTGTATCTGTTCGAACGCG
ACGCCGCGGTGCGCACGCTGCGCTCTGGTTAA

Upstream 100 bases:

>100_bases
ATATATAGGCGTTCTCGGGCGGACTGTCAGCCACCATGGCGGACCCCACCCCCGCCCCCGGGATTTCCCCCTTCCCCTTT
TCCTGGACCCCCGCCCGGCC

Downstream 100 bases:

>100_bases
ACCTCTGTTGGCACAGGATTTTTTTTGACGGAGAGGCTTCCTTGGATTGGATCGTCCTCGGGCGAGGCGGTGACCAGGGG
AACCTTGCGCACCTCCTTGG

Product: ATP-dependent DNA helicase RecG

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 703; Mature: 703

Protein sequence:

>703_residues
MRPSLLDPLFRPLTSLKGAGKTMAPLIARLIGGDKVVDLLWHLPSGLVDRRFSPLIAEAPDGVVVTLTVVVEAHQEPPPR
SPSPYRVVCRDASGFVTLVFFHGRARYLNDLLPVGETRVISGKVERFGGAPQIVHPTHVVPLAEAEAVCRVEPVYPLSGG
VAGKVLARLIAQALDDIPDPAAWPDEWIDAPLKAREGWPGWIDALRAVHAPEEAEDLRPDHPARRRLAYDELLATQLALL
LVRRAARTVRGRPIVGTGALRAKVLAALPFALTGAQSRSLAEIDGDMASPARMLRLLQGDVGSGKTVVALLTMLTAVEAG
AQAALMAPTEILARQHIETLAPLCASAGVRLGLLTGRDKGRARAALLEALAAGEIDILVGTHALFQDDVVFAALAVVVVD
EQHRFGVHQRLALSDKGRAVDVLVMTATPIPRTLTLTHYGDMDISRLDEKPPGRLPADTRVLPIDRLDDVIAAVARAIDG
GAKVYWVCPLIEDSETGDMAAAVDRQALLADRLGPRLGPRVGLVHGRMKPGEKDAVMEAFSGNGLDLLVATTVIEVGVNV
PSATVMVIEHAERFGLAQLHQLRGRIGRGGGRSTCLLLYAPPLGETARARLETMRRTDDGFEIAEEDLRLRGAGEVLGTR
QSGLPVFRLIDPLLAEDLLAIARKQAEVIVETDPDLAGPHGAALRVLLYLFERDAAVRTLRSG

Sequences:

>Translated_703_residues
MRPSLLDPLFRPLTSLKGAGKTMAPLIARLIGGDKVVDLLWHLPSGLVDRRFSPLIAEAPDGVVVTLTVVVEAHQEPPPR
SPSPYRVVCRDASGFVTLVFFHGRARYLNDLLPVGETRVISGKVERFGGAPQIVHPTHVVPLAEAEAVCRVEPVYPLSGG
VAGKVLARLIAQALDDIPDPAAWPDEWIDAPLKAREGWPGWIDALRAVHAPEEAEDLRPDHPARRRLAYDELLATQLALL
LVRRAARTVRGRPIVGTGALRAKVLAALPFALTGAQSRSLAEIDGDMASPARMLRLLQGDVGSGKTVVALLTMLTAVEAG
AQAALMAPTEILARQHIETLAPLCASAGVRLGLLTGRDKGRARAALLEALAAGEIDILVGTHALFQDDVVFAALAVVVVD
EQHRFGVHQRLALSDKGRAVDVLVMTATPIPRTLTLTHYGDMDISRLDEKPPGRLPADTRVLPIDRLDDVIAAVARAIDG
GAKVYWVCPLIEDSETGDMAAAVDRQALLADRLGPRLGPRVGLVHGRMKPGEKDAVMEAFSGNGLDLLVATTVIEVGVNV
PSATVMVIEHAERFGLAQLHQLRGRIGRGGGRSTCLLLYAPPLGETARARLETMRRTDDGFEIAEEDLRLRGAGEVLGTR
QSGLPVFRLIDPLLAEDLLAIARKQAEVIVETDPDLAGPHGAALRVLLYLFERDAAVRTLRSG
>Mature_703_residues
MRPSLLDPLFRPLTSLKGAGKTMAPLIARLIGGDKVVDLLWHLPSGLVDRRFSPLIAEAPDGVVVTLTVVVEAHQEPPPR
SPSPYRVVCRDASGFVTLVFFHGRARYLNDLLPVGETRVISGKVERFGGAPQIVHPTHVVPLAEAEAVCRVEPVYPLSGG
VAGKVLARLIAQALDDIPDPAAWPDEWIDAPLKAREGWPGWIDALRAVHAPEEAEDLRPDHPARRRLAYDELLATQLALL
LVRRAARTVRGRPIVGTGALRAKVLAALPFALTGAQSRSLAEIDGDMASPARMLRLLQGDVGSGKTVVALLTMLTAVEAG
AQAALMAPTEILARQHIETLAPLCASAGVRLGLLTGRDKGRARAALLEALAAGEIDILVGTHALFQDDVVFAALAVVVVD
EQHRFGVHQRLALSDKGRAVDVLVMTATPIPRTLTLTHYGDMDISRLDEKPPGRLPADTRVLPIDRLDDVIAAVARAIDG
GAKVYWVCPLIEDSETGDMAAAVDRQALLADRLGPRLGPRVGLVHGRMKPGEKDAVMEAFSGNGLDLLVATTVIEVGVNV
PSATVMVIEHAERFGLAQLHQLRGRIGRGGGRSTCLLLYAPPLGETARARLETMRRTDDGFEIAEEDLRLRGAGEVLGTR
QSGLPVFRLIDPLLAEDLLAIARKQAEVIVETDPDLAGPHGAALRVLLYLFERDAAVRTLRSG

Specific function: Critical role in recombination and DNA repair. Help process Holliday junction intermediates to mature products by catalyzing branch migration. Has a DNA unwinding activity characteristic of a DNA helicase with a 3' to 5' polarity. RecG unwind branched dup

COG id: COG1200

COG function: function code LK; RecG-like helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367254, Length=691, Percent_Identity=45.1519536903039, Blast_Score=476, Evalue=1e-135,
Organism=Escherichia coli, GI1787357, Length=382, Percent_Identity=38.4816753926702, Blast_Score=246, Evalue=4e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR004609
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C; PF01336 tRNA_anti [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 75369; Mature: 75369

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: PS00435 PEROXIDASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPSLLDPLFRPLTSLKGAGKTMAPLIARLIGGDKVVDLLWHLPSGLVDRRFSPLIAEAP
CCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHHCCCHHHHHCCCCHHHCCC
DGVVVTLTVVVEAHQEPPPRSPSPYRVVCRDASGFVTLVFFHGRARYLNDLLPVGETRVI
CCEEEEEEEEEEECCCCCCCCCCCCEEEEECCCCCEEEEEECCHHHHHHHHCCCCCEEEE
SGKVERFGGAPQIVHPTHVVPLAEAEAVCRVEPVYPLSGGVAGKVLARLIAQALDDIPDP
ECHHHHCCCCCCCCCCCCEEECCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCC
AAWPDEWIDAPLKAREGWPGWIDALRAVHAPEEAEDLRPDHPARRRLAYDELLATQLALL
CCCCHHHHCCCCCCCCCCCHHHHHHHHHCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHH
LVRRAARTVRGRPIVGTGALRAKVLAALPFALTGAQSRSLAEIDGDMASPARMLRLLQGD
HHHHHHHHHCCCCEEECHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCHHHHHHHHHCC
VGSGKTVVALLTMLTAVEAGAQAALMAPTEILARQHIETLAPLCASAGVRLGLLTGRDKG
CCCCHHHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCH
RARAALLEALAAGEIDILVGTHALFQDDVVFAALAVVVVDEQHRFGVHQRLALSDKGRAV
HHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHCCCHHHCCHHHHEEECCCCCEE
DVLVMTATPIPRTLTLTHYGDMDISRLDEKPPGRLPADTRVLPIDRLDDVIAAVARAIDG
EEEEEECCCCCCEEEEEECCCCCHHHCCCCCCCCCCCCCEEECHHHHHHHHHHHHHHHCC
GAKVYWVCPLIEDSETGDMAAAVDRQALLADRLGPRLGPRVGLVHGRMKPGEKDAVMEAF
CCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHHH
SGNGLDLLVATTVIEVGVNVPSATVMVIEHAERFGLAQLHQLRGRIGRGGGRSTCLLLYA
CCCCCCHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEC
PPLGETARARLETMRRTDDGFEIAEEDLRLRGAGEVLGTRQSGLPVFRLIDPLLAEDLLA
CCCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHH
IARKQAEVIVETDPDLAGPHGAALRVLLYLFERDAAVRTLRSG
HHHHCCCEEEECCCCCCCCCHHHHHHHHHHHHCCHHHHHHCCC
>Mature Secondary Structure
MRPSLLDPLFRPLTSLKGAGKTMAPLIARLIGGDKVVDLLWHLPSGLVDRRFSPLIAEAP
CCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHHCCCHHHHHCCCCHHHCCC
DGVVVTLTVVVEAHQEPPPRSPSPYRVVCRDASGFVTLVFFHGRARYLNDLLPVGETRVI
CCEEEEEEEEEEECCCCCCCCCCCCEEEEECCCCCEEEEEECCHHHHHHHHCCCCCEEEE
SGKVERFGGAPQIVHPTHVVPLAEAEAVCRVEPVYPLSGGVAGKVLARLIAQALDDIPDP
ECHHHHCCCCCCCCCCCCEEECCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCC
AAWPDEWIDAPLKAREGWPGWIDALRAVHAPEEAEDLRPDHPARRRLAYDELLATQLALL
CCCCHHHHCCCCCCCCCCCHHHHHHHHHCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHH
LVRRAARTVRGRPIVGTGALRAKVLAALPFALTGAQSRSLAEIDGDMASPARMLRLLQGD
HHHHHHHHHCCCCEEECHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCHHHHHHHHHCC
VGSGKTVVALLTMLTAVEAGAQAALMAPTEILARQHIETLAPLCASAGVRLGLLTGRDKG
CCCCHHHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCH
RARAALLEALAAGEIDILVGTHALFQDDVVFAALAVVVVDEQHRFGVHQRLALSDKGRAV
HHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHCCCHHHCCHHHHEEECCCCCEE
DVLVMTATPIPRTLTLTHYGDMDISRLDEKPPGRLPADTRVLPIDRLDDVIAAVARAIDG
EEEEEECCCCCCEEEEEECCCCCHHHCCCCCCCCCCCCCEEECHHHHHHHHHHHHHHHCC
GAKVYWVCPLIEDSETGDMAAAVDRQALLADRLGPRLGPRVGLVHGRMKPGEKDAVMEAF
CCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHHH
SGNGLDLLVATTVIEVGVNVPSATVMVIEHAERFGLAQLHQLRGRIGRGGGRSTCLLLYA
CCCCCCHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEC
PPLGETARARLETMRRTDDGFEIAEEDLRLRGAGEVLGTRQSGLPVFRLIDPLLAEDLLA
CCCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHH
IARKQAEVIVETDPDLAGPHGAALRVLLYLFERDAAVRTLRSG
HHHHCCCEEEECCCCCCCCCHHHHHHHHHHHHCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]