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The map label for this gene is mltB [H]

Identifier: 83593041

GI number: 83593041

Start: 1996760

End: 1998124

Strand: Reverse

Name: mltB [H]

Synonym: Rru_A1706

Alternate gene names: 83593041

Gene position: 1998124-1996760 (Counterclockwise)

Preceding gene: 83593042

Following gene: 83593040

Centisome position: 45.9

GC content: 71.14

Gene sequence:

>1365_bases
ATGGCACGTCTTGGATCCCCTTATAAAAAGCAAAAGAGTGTCCCCATGGCCCGTCGCCCCTTCGCCCTGGCCGCCCTGCC
GGTGGTTCTTCTCGCCCTGTCCAGTTGCGCCGGCGCCGACCAAAGCCAGCGCTCGGGCGGGACCGGCGGCGGGCGATCGG
CGCCGGGGATGGCGCCGGTGTCGCCCATCGCCCCAGCGGCGGCGCTCAGTCCGGCCGCCGAACAGGCCGCCTTCGCCCAA
TGGCTGGTCGGCATGCGGGCCGAGGCCCTGGCGGCGGGCATCAGCCGCGCCACCGTCGACAGCGCCTTGGGCCGGGCGCG
GATCGAGCCGCGCATCGTCGAGCTTGATCGCAAGCAGCCCGAATTCAACAAGACCCTGGCGGGCTATTTCGCCTCGACCC
TGCCCGACAGCCGCATCGCCAAAGCCCGCGAGATGATGGCGCTGCACGCGCCGTTGCTGCGCTCGCTTGAACAACGCTAT
GGCGTGCCGGGGCGCTTCCTGGTCGCTTTCTGGGGGTTGGAAACCAATTTCGGCGGCACGCTCGGCTCGTTCCGCGTCGT
CGACGCGCTGTCCACCCTGGCCTTCGAGGGCCGGCGCGGCGCCTTCTTCCGGGCCCAGTTGCTTGACGCCTTGCGCATCA
TCGACGCCGGCCATGTCAGCGCCGACGCCATGATCGGCTCGTGGGCCGGGGCGATGGGCCAAACCCAGTTCATGCCCTCG
ACCTTCCGCGCCCATGCGGTCGATTACGATGGCGACGGCCGCATCGACATCTGGGGCAGCCTGCCCGACGCCCTGGCCTC
GGCGGCGAAATACCTGTCCGATCTGGGCTGGAAGGGCGCCGAGCCCTGGGGGTGGGAGGTGGTGCTGCCCGCCGGGCTGG
ATCTGGCGCGGGTCGGCCTGGAAGAACGCCGGCCTTTGGCCGATTGGGCGGCGCTCGGCTTGCGCGGCGCCGATGGCCGG
GCGCTGGGGGCGGGGGCCAGAGCCGGGGTGACGGCCGCCCTGCTGCTGCCGGCGGGCTATCGCGGCCCGGCCTTCCTGGT
CACCGACAACTATTTCTCTATCCTCAAATGGAACCGCTCGATGCTCTATGCCCTGGCCGTCGGGCTCTTGGCCGATCGGG
CGGCGGGCGGCGGGCCGCTGGTCCATCCGCCGCCGGCCGACGAGGCGCCACTGCGCAGCGCCGATGTCGCCGAGATCCAA
AGCCGCCTGTCCGCCCTGGGCTATGACGCCGGCACGGCCGATGGCGTGATCGGCGCCCAGACCCGGGTCGCCATCCGCGC
CTATCAAGCTTCGATCGGCGCCCCGGCCGATGGCTATGCCAATGACGCCCTGCTCGCCGATCTGCGCCGGCGGAGTGGCC
GATGA

Upstream 100 bases:

>100_bases
AGGGGGACGGGGAGGGGTGTCAACGGGGGGTTCTTGGGGGAGGGGGCTTTGGGCGGGATCGCGGTTTCCTTGCCCATGGG
TTGGACATTGCCGGTGGTTT

Downstream 100 bases:

>100_bases
GCGTGAAGAGTTGGCGCGGCGGGCGAAGCTGGCGGGCGCCGCTTGGTCTCTTGCTGATCAGCGCCGCCGCCCTCGCCCTG
TCGGGCTGCGCCGAAACCGG

Product: lytic murein transglycosylase

Products: Muramic Acid Residue [C]

Alternate protein names: 35 kDa soluble lytic transglycosylase; Murein hydrolase B; Slt35 [H]

Number of amino acids: Translated: 454; Mature: 453

Protein sequence:

>454_residues
MARLGSPYKKQKSVPMARRPFALAALPVVLLALSSCAGADQSQRSGGTGGGRSAPGMAPVSPIAPAAALSPAAEQAAFAQ
WLVGMRAEALAAGISRATVDSALGRARIEPRIVELDRKQPEFNKTLAGYFASTLPDSRIAKAREMMALHAPLLRSLEQRY
GVPGRFLVAFWGLETNFGGTLGSFRVVDALSTLAFEGRRGAFFRAQLLDALRIIDAGHVSADAMIGSWAGAMGQTQFMPS
TFRAHAVDYDGDGRIDIWGSLPDALASAAKYLSDLGWKGAEPWGWEVVLPAGLDLARVGLEERRPLADWAALGLRGADGR
ALGAGARAGVTAALLLPAGYRGPAFLVTDNYFSILKWNRSMLYALAVGLLADRAAGGGPLVHPPPADEAPLRSADVAEIQ
SRLSALGYDAGTADGVIGAQTRVAIRAYQASIGAPADGYANDALLADLRRRSGR

Sequences:

>Translated_454_residues
MARLGSPYKKQKSVPMARRPFALAALPVVLLALSSCAGADQSQRSGGTGGGRSAPGMAPVSPIAPAAALSPAAEQAAFAQ
WLVGMRAEALAAGISRATVDSALGRARIEPRIVELDRKQPEFNKTLAGYFASTLPDSRIAKAREMMALHAPLLRSLEQRY
GVPGRFLVAFWGLETNFGGTLGSFRVVDALSTLAFEGRRGAFFRAQLLDALRIIDAGHVSADAMIGSWAGAMGQTQFMPS
TFRAHAVDYDGDGRIDIWGSLPDALASAAKYLSDLGWKGAEPWGWEVVLPAGLDLARVGLEERRPLADWAALGLRGADGR
ALGAGARAGVTAALLLPAGYRGPAFLVTDNYFSILKWNRSMLYALAVGLLADRAAGGGPLVHPPPADEAPLRSADVAEIQ
SRLSALGYDAGTADGVIGAQTRVAIRAYQASIGAPADGYANDALLADLRRRSGR
>Mature_453_residues
ARLGSPYKKQKSVPMARRPFALAALPVVLLALSSCAGADQSQRSGGTGGGRSAPGMAPVSPIAPAAALSPAAEQAAFAQW
LVGMRAEALAAGISRATVDSALGRARIEPRIVELDRKQPEFNKTLAGYFASTLPDSRIAKAREMMALHAPLLRSLEQRYG
VPGRFLVAFWGLETNFGGTLGSFRVVDALSTLAFEGRRGAFFRAQLLDALRIIDAGHVSADAMIGSWAGAMGQTQFMPST
FRAHAVDYDGDGRIDIWGSLPDALASAAKYLSDLGWKGAEPWGWEVVLPAGLDLARVGLEERRPLADWAALGLRGADGRA
LGAGARAGVTAALLLPAGYRGPAFLVTDNYFSILKWNRSMLYALAVGLLADRAAGGGPLVHPPPADEAPLRSADVAEIQS
RLSALGYDAGTADGVIGAQTRVAIRAYQASIGAPADGYANDALLADLRRRSGR

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG2951

COG function: function code M; Membrane-bound lytic murein transglycosylase B

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor; Periplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789053, Length=221, Percent_Identity=33.0316742081448, Blast_Score=100, Evalue=3e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011757 [H]

Pfam domain/function: NA

EC number: 3.2.1.- [C]

Molecular weight: Translated: 47615; Mature: 47484

Theoretical pI: Translated: 9.98; Mature: 9.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARLGSPYKKQKSVPMARRPFALAALPVVLLALSSCAGADQSQRSGGTGGGRSAPGMAPV
CCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCCCCCCCCCCC
SPIAPAAALSPAAEQAAFAQWLVGMRAEALAAGISRATVDSALGRARIEPRIVELDRKQP
CCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCC
EFNKTLAGYFASTLPDSRIAKAREMMALHAPLLRSLEQRYGVPGRFLVAFWGLETNFGGT
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCC
LGSFRVVDALSTLAFEGRRGAFFRAQLLDALRIIDAGHVSADAMIGSWAGAMGQTQFMPS
CCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCC
TFRAHAVDYDGDGRIDIWGSLPDALASAAKYLSDLGWKGAEPWGWEVVLPAGLDLARVGL
HHHHEEECCCCCCCEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCHHHHHCH
EERRPLADWAALGLRGADGRALGAGARAGVTAALLLPAGYRGPAFLVTDNYFSILKWNRS
HHCCCHHHHHHHCCCCCCCCEECCCCHHCCEEEEEECCCCCCCEEEEECCCHHHHHHHHH
MLYALAVGLLADRAAGGGPLVHPPPADEAPLRSADVAEIQSRLSALGYDAGTADGVIGAQ
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCH
TRVAIRAYQASIGAPADGYANDALLADLRRRSGR
HHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure 
ARLGSPYKKQKSVPMARRPFALAALPVVLLALSSCAGADQSQRSGGTGGGRSAPGMAPV
CCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCCCCCCCCCCC
SPIAPAAALSPAAEQAAFAQWLVGMRAEALAAGISRATVDSALGRARIEPRIVELDRKQP
CCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCC
EFNKTLAGYFASTLPDSRIAKAREMMALHAPLLRSLEQRYGVPGRFLVAFWGLETNFGGT
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCC
LGSFRVVDALSTLAFEGRRGAFFRAQLLDALRIIDAGHVSADAMIGSWAGAMGQTQFMPS
CCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCC
TFRAHAVDYDGDGRIDIWGSLPDALASAAKYLSDLGWKGAEPWGWEVVLPAGLDLARVGL
HHHHEEECCCCCCCEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCHHHHHCH
EERRPLADWAALGLRGADGRALGAGARAGVTAALLLPAGYRGPAFLVTDNYFSILKWNRS
HHCCCHHHHHHHCCCCCCCCEECCCCHHCCEEEEEECCCCCCCEEEEECCCHHHHHHHHH
MLYALAVGLLADRAAGGGPLVHPPPADEAPLRSADVAEIQSRLSALGYDAGTADGVIGAQ
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCH
TRVAIRAYQASIGAPADGYANDALLADLRRRSGR
HHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

General reaction: Cleavage Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7476170; 7789526; 9205837; 9278503; 3553176; 9761817; 10570954; 10545329; 10684641 [H]