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The map label for this gene is holB [H]

Identifier: 83593037

GI number: 83593037

Start: 1992525

End: 1993649

Strand: Reverse

Name: holB [H]

Synonym: Rru_A1702

Alternate gene names: 83593037

Gene position: 1993649-1992525 (Counterclockwise)

Preceding gene: 83593038

Following gene: 83593036

Centisome position: 45.8

GC content: 71.56

Gene sequence:

>1125_bases
ATGGCCAGCGCGAGCGAAGACGAGGCCGCTGGCGATCCTGGCCCGCGCCTGACCGCCGACCTGCTGGGTCACGAGGCGGC
CGAGCGCGAGATCCTGGAGACCTGCGCCTCGGGCCGCGCCGCCCATGCCTGGCTGATCAGCGGGCCGCGCGGCATCGGCA
AGGCCACCCTGGCCTTTCGTTTCGCCCGCTTCGCCCTGGCCGGCGGCCTAGAGGGCGGGGAGGGCGGATTGTTTGGCGCT
CTGGCGCCGGCCTCGCTGGCCATGGATCCGGCCCATCCGGTGTTTCGCCGGGTGGCCGCCGGGTCGCACGGTGACCTGCG
GGTGATCGAGCGCGGTCTGGCCGAAGGGACCGGCGGCCGGCGGCGCGGCGAGATCGTCATCGGCGATGTGCGCGATCTGG
GCGGCTTCATGCGCATGACGCCCTCGGAAGGCGGCTGGCGGGTGGTCATCGTCGATGGCGCCGAGGATATGAACCGCAAC
GCCGCCAACGCCTTGCTCAAAGGCCTGGAAGAGCCGCCGGGGCGGGCCCTGCTGCTGCTGGTCAGCCATAATCCGGCCCG
CCTGCTGCCGACCATCCGCTCGCGCTGCCGCCGGCTGGCCCTGCGCCCGCTGGAGCCGGCGGTGCTGGCAACCCTGATCG
AACGGTCTTTGCCCGAGCTTGCCGAAACGGAGCGTCCCGGGTTGATCGGTCTGGCCGAAGGCAGCATCGGCCGCGCCATG
GATCTGGCGGCGATGGGCGGGCTGGCTTTGTGCCGCGAGATGATCGGATTGCTGGCCACCGCGCCGGCCTTTGATGTCGC
CCGCCTGCATGGGTTCGCCGATCAGGCGGCGCGCGACGACCGCGCCTTCACCGCCACCGCCGGCTTGTTCCCCTGGTGGC
TGGCCCGCATGATCCGCCGGGGGGCGGTGGCCGGTTCGGCCGGGACGGCAGAGGAGATGTGGACCATCCCCGGGGAAAGC
GCGGCCTCGGGCCGTCTGCTGGCGGCGGCCACCCTTGATCGCTGGGTCGAGGTATGGGAAAAGGTCACAACCCTTTTCGC
CCGGGCCGAGGCGGTTCACCTTGATCGCAAACAGACGGTTCTCGCCGCCTTTCACGCGGTCGCCTTGGTCGCGGGTCGTT
CCTGA

Upstream 100 bases:

>100_bases
GCTGCCGGCTGATCGAGGCCGATGGCGCGATCGAGGCGGTTCACGCCCGGCTCTGGGCGGTGGTGCTGGAGCGCTTCCCC
GAGTTGACGGAGCGCCGGTG

Downstream 100 bases:

>100_bases
GCGGAGGGGCCTTGCCATTCCCGGGGGATCCCCCGGTCAGGGTTCGGGGTTATCATCCCGAACGTCCCCCCACGTCGCCT
TGCGGAGTTTCCGATCATGG

Product: DNA polymerase III subunit delta'

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 374; Mature: 373

Protein sequence:

>374_residues
MASASEDEAAGDPGPRLTADLLGHEAAEREILETCASGRAAHAWLISGPRGIGKATLAFRFARFALAGGLEGGEGGLFGA
LAPASLAMDPAHPVFRRVAAGSHGDLRVIERGLAEGTGGRRRGEIVIGDVRDLGGFMRMTPSEGGWRVVIVDGAEDMNRN
AANALLKGLEEPPGRALLLLVSHNPARLLPTIRSRCRRLALRPLEPAVLATLIERSLPELAETERPGLIGLAEGSIGRAM
DLAAMGGLALCREMIGLLATAPAFDVARLHGFADQAARDDRAFTATAGLFPWWLARMIRRGAVAGSAGTAEEMWTIPGES
AASGRLLAAATLDRWVEVWEKVTTLFARAEAVHLDRKQTVLAAFHAVALVAGRS

Sequences:

>Translated_374_residues
MASASEDEAAGDPGPRLTADLLGHEAAEREILETCASGRAAHAWLISGPRGIGKATLAFRFARFALAGGLEGGEGGLFGA
LAPASLAMDPAHPVFRRVAAGSHGDLRVIERGLAEGTGGRRRGEIVIGDVRDLGGFMRMTPSEGGWRVVIVDGAEDMNRN
AANALLKGLEEPPGRALLLLVSHNPARLLPTIRSRCRRLALRPLEPAVLATLIERSLPELAETERPGLIGLAEGSIGRAM
DLAAMGGLALCREMIGLLATAPAFDVARLHGFADQAARDDRAFTATAGLFPWWLARMIRRGAVAGSAGTAEEMWTIPGES
AASGRLLAAATLDRWVEVWEKVTTLFARAEAVHLDRKQTVLAAFHAVALVAGRS
>Mature_373_residues
ASASEDEAAGDPGPRLTADLLGHEAAEREILETCASGRAAHAWLISGPRGIGKATLAFRFARFALAGGLEGGEGGLFGAL
APASLAMDPAHPVFRRVAAGSHGDLRVIERGLAEGTGGRRRGEIVIGDVRDLGGFMRMTPSEGGWRVVIVDGAEDMNRNA
ANALLKGLEEPPGRALLLLVSHNPARLLPTIRSRCRRLALRPLEPAVLATLIERSLPELAETERPGLIGLAEGSIGRAMD
LAAMGGLALCREMIGLLATAPAFDVARLHGFADQAARDDRAFTATAGLFPWWLARMIRRGAVAGSAGTAEEMWTIPGESA
ASGRLLAAATLDRWVEVWEKVTTLFARAEAVHLDRKQTVLAAFHAVALVAGRS

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity [H]

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786676, Length=232, Percent_Identity=31.4655172413793, Blast_Score=86, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003959
- InterPro:   IPR004622 [H]

Pfam domain/function: PF00004 AAA [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 39412; Mature: 39281

Theoretical pI: Translated: 7.26; Mature: 7.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASASEDEAAGDPGPRLTADLLGHEAAEREILETCASGRAAHAWLISGPRGIGKATLAFR
CCCCCCCCCCCCCCCCHHHHHHCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHH
FARFALAGGLEGGEGGLFGALAPASLAMDPAHPVFRRVAAGSHGDLRVIERGLAEGTGGR
HHHHHHHCCCCCCCCCCCHHCCCHHHCCCCCHHHHHHHHCCCCCCHHHHHHHHHCCCCCC
RRGEIVIGDVRDLGGFMRMTPSEGGWRVVIVDGAEDMNRNAANALLKGLEEPPGRALLLL
EECCEEEECHHHCCCCEEECCCCCCEEEEEEECCHHHCCHHHHHHHHHCCCCCCCEEEEE
VSHNPARLLPTIRSRCRRLALRPLEPAVLATLIERSLPELAETERPGLIGLAEGSIGRAM
EECCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCHHHHHCCCCCEEEECCCCCCHHH
DLAAMGGLALCREMIGLLATAPAFDVARLHGFADQAARDDRAFTATAGLFPWWLARMIRR
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHH
GAVAGSAGTAEEMWTIPGESAASGRLLAAATLDRWVEVWEKVTTLFARAEAVHLDRKQTV
CCCCCCCCCHHHHEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
LAAFHAVALVAGRS
HHHHHHHHHHCCCC
>Mature Secondary Structure 
ASASEDEAAGDPGPRLTADLLGHEAAEREILETCASGRAAHAWLISGPRGIGKATLAFR
CCCCCCCCCCCCCCCHHHHHHCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHH
FARFALAGGLEGGEGGLFGALAPASLAMDPAHPVFRRVAAGSHGDLRVIERGLAEGTGGR
HHHHHHHCCCCCCCCCCCHHCCCHHHCCCCCHHHHHHHHCCCCCCHHHHHHHHHCCCCCC
RRGEIVIGDVRDLGGFMRMTPSEGGWRVVIVDGAEDMNRNAANALLKGLEEPPGRALLLL
EECCEEEECHHHCCCCEEECCCCCCEEEEEEECCHHHCCHHHHHHHHHCCCCCCCEEEEE
VSHNPARLLPTIRSRCRRLALRPLEPAVLATLIERSLPELAETERPGLIGLAEGSIGRAM
EECCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCHHHHHCCCCCEEEECCCCCCHHH
DLAAMGGLALCREMIGLLATAPAFDVARLHGFADQAARDDRAFTATAGLFPWWLARMIRR
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHH
GAVAGSAGTAEEMWTIPGESAASGRLLAAATLDRWVEVWEKVTTLFARAEAVHLDRKQTV
CCCCCCCCCHHHHEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
LAAFHAVALVAGRS
HHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8550441; 10984043 [H]