Click here to switch to the map view.

The map label for this gene is tmk [H]

Identifier: 83593038

GI number: 83593038

Start: 1993649

End: 1994293

Strand: Reverse

Name: tmk [H]

Synonym: Rru_A1703

Alternate gene names: 83593038

Gene position: 1994293-1993649 (Counterclockwise)

Preceding gene: 83593039

Following gene: 83593037

Centisome position: 45.82

GC content: 72.71

Gene sequence:

>645_bases
GTGACCGGCGGACGGTTCATCACCTTGGAAGGCGGCGAGGGCGCGGGCAAGACCACCCAACTCAGCCGTCTCGCCCAAGC
CCTCGCCCCCCTGGTGCCCGAGGTGGTGACCACCCGCGAGCCCGGGGGATCGCCCGGCGCCGAGGCCATCCGCGGCCTTC
TGGTGGCGGGGGGGGCGGAGCGCTGGGATCCCCGGGGCGAAGCCCTGCTCCATGCCGCCGCCCGTCGCGATCACCTGCTG
CGCACGGTCTGGCCGGCGCTGGCCCGGGGCGCCTGGGTGGTCTGCGACCGCTTCGCCGATTCAACCTCGGCCTATCAGGG
CGGCGGCCATGGCCTGCCCCAGGACGAGCTCGACGCCCTGCACGCCTTTGTTTGCGGCGGCTTCCAGCCCGACTTGACCG
TGATCTTCGACCTGCCGGTGGCCGTGGGATTGGCCCGCGCCGGCCGACGCGGCGGCGGCGAAGACCGCTATGAACGCATG
GACCCGGCCTTCCATCAACGGCTGTTCGACCGCTACCACGCCATCGCCAGCGCTTTCCCCGAACGCTGCCGGCTGATCGA
GGCCGATGGCGCGATCGAGGCGGTTCACGCCCGGCTCTGGGCGGTGGTGCTGGAGCGCTTCCCCGAGTTGACGGAGCGCC
GGTGA

Upstream 100 bases:

>100_bases
CAGCTGTCGTTCTTCGGGCGCATCCTGGCCTCGGCGCGCAATGCGGTGTTCGGCACCGCCCAGCCCGCCGCCCACTAGGC
CGATGAAGCGGGAAACGCCG

Downstream 100 bases:

>100_bases
TGGCCAGCGCGAGCGAAGACGAGGCCGCTGGCGATCCTGGCCCGCGCCTGACCGCCGACCTGCTGGGTCACGAGGCGGCC
GAGCGCGAGATCCTGGAGAC

Product: thymidylate kinase

Products: NA

Alternate protein names: dTMP kinase [H]

Number of amino acids: Translated: 214; Mature: 213

Protein sequence:

>214_residues
MTGGRFITLEGGEGAGKTTQLSRLAQALAPLVPEVVTTREPGGSPGAEAIRGLLVAGGAERWDPRGEALLHAAARRDHLL
RTVWPALARGAWVVCDRFADSTSAYQGGGHGLPQDELDALHAFVCGGFQPDLTVIFDLPVAVGLARAGRRGGGEDRYERM
DPAFHQRLFDRYHAIASAFPERCRLIEADGAIEAVHARLWAVVLERFPELTERR

Sequences:

>Translated_214_residues
MTGGRFITLEGGEGAGKTTQLSRLAQALAPLVPEVVTTREPGGSPGAEAIRGLLVAGGAERWDPRGEALLHAAARRDHLL
RTVWPALARGAWVVCDRFADSTSAYQGGGHGLPQDELDALHAFVCGGFQPDLTVIFDLPVAVGLARAGRRGGGEDRYERM
DPAFHQRLFDRYHAIASAFPERCRLIEADGAIEAVHARLWAVVLERFPELTERR
>Mature_213_residues
TGGRFITLEGGEGAGKTTQLSRLAQALAPLVPEVVTTREPGGSPGAEAIRGLLVAGGAERWDPRGEALLHAAARRDHLLR
TVWPALARGAWVVCDRFADSTSAYQGGGHGLPQDELDALHAFVCGGFQPDLTVIFDLPVAVGLARAGRRGGGEDRYERMD
PAFHQRLFDRYHAIASAFPERCRLIEADGAIEAVHARLWAVVLERFPELTERR

Specific function: Phosphorylation of dTMP to form dTDP in both de novo and salvage pathways of dTTP synthesis [H]

COG id: COG0125

COG function: function code F; Thymidylate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate kinase family [H]

Homologues:

Organism=Escherichia coli, GI1787340, Length=211, Percent_Identity=39.3364928909953, Blast_Score=118, Evalue=4e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018095
- InterPro:   IPR018094 [H]

Pfam domain/function: PF02223 Thymidylate_kin [H]

EC number: =2.7.4.9 [H]

Molecular weight: Translated: 23170; Mature: 23038

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: PS01331 THYMIDYLATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGGRFITLEGGEGAGKTTQLSRLAQALAPLVPEVVTTREPGGSPGAEAIRGLLVAGGAE
CCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCC
RWDPRGEALLHAAARRDHLLRTVWPALARGAWVVCDRFADSTSAYQGGGHGLPQDELDAL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEHHHHHCCCCCCCCCCCCCCHHHHHHH
HAFVCGGFQPDLTVIFDLPVAVGLARAGRRGGGEDRYERMDPAFHQRLFDRYHAIASAFP
HHHHHCCCCCCEEEEEHHHHHHHHHHHCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHH
ERCRLIEADGAIEAVHARLWAVVLERFPELTERR
HHHEEECCCCHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TGGRFITLEGGEGAGKTTQLSRLAQALAPLVPEVVTTREPGGSPGAEAIRGLLVAGGAE
CCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCC
RWDPRGEALLHAAARRDHLLRTVWPALARGAWVVCDRFADSTSAYQGGGHGLPQDELDAL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEHHHHHCCCCCCCCCCCCCCHHHHHHH
HAFVCGGFQPDLTVIFDLPVAVGLARAGRRGGGEDRYERMDPAFHQRLFDRYHAIASAFP
HHHHHCCCCCCEEEEEHHHHHHHHHHHCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHH
ERCRLIEADGAIEAVHARLWAVVLERFPELTERR
HHHEEECCCCHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11259647 [H]