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The map label for this gene is metG [H]

Identifier: 83593036

GI number: 83593036

Start: 1990869

End: 1992428

Strand: Reverse

Name: metG [H]

Synonym: Rru_A1701

Alternate gene names: 83593036

Gene position: 1992428-1990869 (Counterclockwise)

Preceding gene: 83593037

Following gene: 83593035

Centisome position: 45.77

GC content: 65.06

Gene sequence:

>1560_bases
ATGGCCGGGCCCAAGCCCCCGTTTTATCTAACGACGCCGATCTACTATGTGAACGACAAGCCCCATATCGGCCATGCCTA
TACCACCCTTGCCTGCGACGTGCTGGCGCGGTTCAAGCGCCTGGACGGCTTCGATGTCAAATTCCTGACCGGAACCGACG
AGCACGGCCAGAAGGTGCAGAAATCGGCCACGGCGGCCGGCATCGATCCGCAGACCTTCACCGACCGGGTCTCGGCCAAT
TTCCGCGATCTGGCCGCGGCGATGAATTTCTCCAACGACGCCTTCATTCGCACCACCGAGCCCCGCCATAAGGAGGCCTG
CAAGGCCCTGTGGACCCTGCTGGTCGAGCGCGGCGAGATTTATCTTGACGCCTATCGCGGCTGGTACGCGGTGCGCGACG
AGGCCTTCTATGGCGAAGACGAACTGACCACGGCCGCCGATGGCAGCCGCCTAGCCCCCACCGGCGCGCCGGTGGAGTGG
GTGGAGGAGCCGAGCTATTTCTTCCGGCTGTCGAAATGGCAGCAGCCGCTGCTTGATCTTTACGCCGCCCGCCCCGATTT
CATCGCCCCCGACAGCCGGCGCAACGAGGTCATCAGCTTCGTCAAGGGCGGGCTGACCGATTTGTCGATCTCGCGCACCA
CCTTTTCCTGGGGCGTGCCGGTGCCCGGCGACGAAGACCATGTGATGTATGTCTGGCTTGACGCCCTGACCAATTACATC
ACCGCCGTCGGCTTCCCCGATCAGACCGGCGACTATGCCCGCTATTGGCCGGCCAACTTGCATATGGTCGGCAAGGACAT
CTTGCGCTTCCATACCGTCTATTGGCCGGCCTTCCTGATGGCCGCCGGCCTGGAGGTTCCGGGCCGGGTTTTCGCCCATG
GCTGGTGGACCAACGAGGGGCAGAAGATCTCCAAATCGGTGGGCAACGTCATCGATCCGCGGGCGCTGATCGAGACCTAT
GGCCTTGATCCGGTCCGCTATTTCCTGCTGCGTGAGGTTCCCTTCGGCAATGATGGCGATTTCAGCCATCGCGCCATGAT
CAACCGCATGAACGGCGAATTGGCCAATGACCTGGGCAATCTCGCCCAGCGCGTGCTGTCGATGATCGCCAAGAACTGCG
CCGGCGCCGTACCCGAACCCGGGCCCTTCAGCGAAGACGACGACGCCCTGTTGGCCGCCGCCGTCGCCCTGCTCGACAAG
GTCCGTCAGGCGATGGACCGTCAGGCCTTCCACGAGGCCTTGGAGGCGATCTGGGTGGTGATCCGCGCCGCCAACGGCTA
TGTCGACCGTCAGGCGCCCTGGGCCTTGCGCAAAAACGATCCGCCGCGCATGGCCACCGTGCTTTATGTGCTGGCCGAGG
TCATCCGCCGCGTCGCCCTGCTGATGCAGCCCTTCGTTCCCGCCTCGGCCGCCCGCATGCTCGACCAGTTGGGGCAGGGG
CCCGAGGCCCGCGATACGACGGCGCTGGGCGATCGGCCGCTGCTGGCGCCGGGCACCGTCCTGCCCAAGCCCGAAGGCAT
CTTCCCCCGCCACGTGGTGGACGACGAGGGGGCGGCGTGA

Upstream 100 bases:

>100_bases
CTGAGCGGAGGGGCCTTGCCATTCCCGGGGGATCCCCCGGTCAGGGTTCGGGGTTATCATCCCGAACGTCCCCCCACGTC
GCCTTGCGGAGTTTCCGATC

Downstream 100 bases:

>100_bases
GCGCCCCCTTCCACCCTTGTTTTCTCCTAGTAGAGTAATGGATAGCCATGTCCCATGGTGGTCACCGATGATCGTCGATA
GCCATTGCCACCTCGACTTC

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS [H]

Number of amino acids: Translated: 519; Mature: 518

Protein sequence:

>519_residues
MAGPKPPFYLTTPIYYVNDKPHIGHAYTTLACDVLARFKRLDGFDVKFLTGTDEHGQKVQKSATAAGIDPQTFTDRVSAN
FRDLAAAMNFSNDAFIRTTEPRHKEACKALWTLLVERGEIYLDAYRGWYAVRDEAFYGEDELTTAADGSRLAPTGAPVEW
VEEPSYFFRLSKWQQPLLDLYAARPDFIAPDSRRNEVISFVKGGLTDLSISRTTFSWGVPVPGDEDHVMYVWLDALTNYI
TAVGFPDQTGDYARYWPANLHMVGKDILRFHTVYWPAFLMAAGLEVPGRVFAHGWWTNEGQKISKSVGNVIDPRALIETY
GLDPVRYFLLREVPFGNDGDFSHRAMINRMNGELANDLGNLAQRVLSMIAKNCAGAVPEPGPFSEDDDALLAAAVALLDK
VRQAMDRQAFHEALEAIWVVIRAANGYVDRQAPWALRKNDPPRMATVLYVLAEVIRRVALLMQPFVPASAARMLDQLGQG
PEARDTTALGDRPLLAPGTVLPKPEGIFPRHVVDDEGAA

Sequences:

>Translated_519_residues
MAGPKPPFYLTTPIYYVNDKPHIGHAYTTLACDVLARFKRLDGFDVKFLTGTDEHGQKVQKSATAAGIDPQTFTDRVSAN
FRDLAAAMNFSNDAFIRTTEPRHKEACKALWTLLVERGEIYLDAYRGWYAVRDEAFYGEDELTTAADGSRLAPTGAPVEW
VEEPSYFFRLSKWQQPLLDLYAARPDFIAPDSRRNEVISFVKGGLTDLSISRTTFSWGVPVPGDEDHVMYVWLDALTNYI
TAVGFPDQTGDYARYWPANLHMVGKDILRFHTVYWPAFLMAAGLEVPGRVFAHGWWTNEGQKISKSVGNVIDPRALIETY
GLDPVRYFLLREVPFGNDGDFSHRAMINRMNGELANDLGNLAQRVLSMIAKNCAGAVPEPGPFSEDDDALLAAAVALLDK
VRQAMDRQAFHEALEAIWVVIRAANGYVDRQAPWALRKNDPPRMATVLYVLAEVIRRVALLMQPFVPASAARMLDQLGQG
PEARDTTALGDRPLLAPGTVLPKPEGIFPRHVVDDEGAA
>Mature_518_residues
AGPKPPFYLTTPIYYVNDKPHIGHAYTTLACDVLARFKRLDGFDVKFLTGTDEHGQKVQKSATAAGIDPQTFTDRVSANF
RDLAAAMNFSNDAFIRTTEPRHKEACKALWTLLVERGEIYLDAYRGWYAVRDEAFYGEDELTTAADGSRLAPTGAPVEWV
EEPSYFFRLSKWQQPLLDLYAARPDFIAPDSRRNEVISFVKGGLTDLSISRTTFSWGVPVPGDEDHVMYVWLDALTNYIT
AVGFPDQTGDYARYWPANLHMVGKDILRFHTVYWPAFLMAAGLEVPGRVFAHGWWTNEGQKISKSVGNVIDPRALIETYG
LDPVRYFLLREVPFGNDGDFSHRAMINRMNGELANDLGNLAQRVLSMIAKNCAGAVPEPGPFSEDDDALLAAAVALLDKV
RQAMDRQAFHEALEAIWVVIRAANGYVDRQAPWALRKNDPPRMATVLYVLAEVIRRVALLMQPFVPASAARMLDQLGQGP
EARDTTALGDRPLLAPGTVLPKPEGIFPRHVVDDEGAA

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [H]

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 2B subfamily [H]

Homologues:

Organism=Homo sapiens, GI24308436, Length=508, Percent_Identity=40.1574803149606, Blast_Score=379, Evalue=1e-105,
Organism=Homo sapiens, GI14043022, Length=518, Percent_Identity=27.7992277992278, Blast_Score=160, Evalue=3e-39,
Organism=Escherichia coli, GI1788432, Length=501, Percent_Identity=24.9500998003992, Blast_Score=155, Evalue=6e-39,
Organism=Caenorhabditis elegans, GI212645096, Length=503, Percent_Identity=35.5864811133201, Blast_Score=328, Evalue=5e-90,
Organism=Caenorhabditis elegans, GI71992781, Length=390, Percent_Identity=32.5641025641026, Blast_Score=224, Evalue=7e-59,
Organism=Caenorhabditis elegans, GI17540794, Length=513, Percent_Identity=28.0701754385965, Blast_Score=156, Evalue=2e-38,
Organism=Saccharomyces cerevisiae, GI6321610, Length=523, Percent_Identity=33.4608030592734, Blast_Score=298, Evalue=1e-81,
Organism=Saccharomyces cerevisiae, GI6321704, Length=555, Percent_Identity=25.045045045045, Blast_Score=129, Evalue=9e-31,
Organism=Drosophila melanogaster, GI21358483, Length=531, Percent_Identity=36.7231638418079, Blast_Score=345, Evalue=3e-95,
Organism=Drosophila melanogaster, GI24655311, Length=560, Percent_Identity=26.0714285714286, Blast_Score=152, Evalue=6e-37,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR015413
- InterPro:   IPR014758
- InterPro:   IPR014729
- InterPro:   IPR009080 [H]

Pfam domain/function: PF09334 tRNA-synt_1g [H]

EC number: =6.1.1.10 [H]

Molecular weight: Translated: 57765; Mature: 57634

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGPKPPFYLTTPIYYVNDKPHIGHAYTTLACDVLARFKRLDGFDVKFLTGTDEHGQKVQ
CCCCCCCEEEECCEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHH
KSATAAGIDPQTFTDRVSANFRDLAAAMNFSNDAFIRTTEPRHKEACKALWTLLVERGEI
HHHHHCCCCCHHHHHHHHCCHHHHHHHHCCCCCCEEECCCCHHHHHHHHHHHHHHHCCCE
YLDAYRGWYAVRDEAFYGEDELTTAADGSRLAPTGAPVEWVEEPSYFFRLSKWQQPLLDL
EEEECCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHH
YAARPDFIAPDSRRNEVISFVKGGLTDLSISRTTFSWGVPVPGDEDHVMYVWLDALTNYI
HHCCCCCCCCCCHHHHHHHHHHCCCCEEEEECEEEECCCCCCCCCCCEEEEEHHHHHHHH
TAVGFPDQTGDYARYWPANLHMVGKDILRFHTVYWPAFLMAAGLEVPGRVFAHGWWTNEG
HCCCCCCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECEECCCC
QKISKSVGNVIDPRALIETYGLDPVRYFLLREVPFGNDGDFSHRAMINRMNGELANDLGN
HHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHCCHHHHHHHH
LAQRVLSMIAKNCAGAVPEPGPFSEDDDALLAAAVALLDKVRQAMDRQAFHEALEAIWVV
HHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IRAANGYVDRQAPWALRKNDPPRMATVLYVLAEVIRRVALLMQPFVPASAARMLDQLGQG
HHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCC
PEARDTTALGDRPLLAPGTVLPKPEGIFPRHVVDDEGAA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AGPKPPFYLTTPIYYVNDKPHIGHAYTTLACDVLARFKRLDGFDVKFLTGTDEHGQKVQ
CCCCCCEEEECCEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHH
KSATAAGIDPQTFTDRVSANFRDLAAAMNFSNDAFIRTTEPRHKEACKALWTLLVERGEI
HHHHHCCCCCHHHHHHHHCCHHHHHHHHCCCCCCEEECCCCHHHHHHHHHHHHHHHCCCE
YLDAYRGWYAVRDEAFYGEDELTTAADGSRLAPTGAPVEWVEEPSYFFRLSKWQQPLLDL
EEEECCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHH
YAARPDFIAPDSRRNEVISFVKGGLTDLSISRTTFSWGVPVPGDEDHVMYVWLDALTNYI
HHCCCCCCCCCCHHHHHHHHHHCCCCEEEEECEEEECCCCCCCCCCCEEEEEHHHHHHHH
TAVGFPDQTGDYARYWPANLHMVGKDILRFHTVYWPAFLMAAGLEVPGRVFAHGWWTNEG
HCCCCCCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECEECCCC
QKISKSVGNVIDPRALIETYGLDPVRYFLLREVPFGNDGDFSHRAMINRMNGELANDLGN
HHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHCCHHHHHHHH
LAQRVLSMIAKNCAGAVPEPGPFSEDDDALLAAAVALLDKVRQAMDRQAFHEALEAIWVV
HHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IRAANGYVDRQAPWALRKNDPPRMATVLYVLAEVIRRVALLMQPFVPASAARMLDQLGQG
HHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCC
PEARDTTALGDRPLLAPGTVLPKPEGIFPRHVVDDEGAA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11214968 [H]