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The map label for this gene is dus [H]

Identifier: 83593011

GI number: 83593011

Start: 1965546

End: 1966634

Strand: Direct

Name: dus [H]

Synonym: Rru_A1676

Alternate gene names: 83593011

Gene position: 1965546-1966634 (Clockwise)

Preceding gene: 83593010

Following gene: 83593012

Centisome position: 45.16

GC content: 71.07

Gene sequence:

>1089_bases
ATGACGCCGTCCCCCGCGTTCCCCGTTTCCGATCCGCTGATCATGCTGGCGCCGATGGCTGGGGTAACCGATTCCCCCTT
CCGGCGACTGGCCCACGACTATGGGTGTCCGCTATCGTGGACCGAGATGGTCGCCAGCCGCGAGCTGCTGCGCGAGCGGG
CGCGGGGCGTCGATCCCCGGCTGGGCGGCGGAGCGGAGGCCGGGCGGGCGGGCCCCTTCGCCGTGCAACTGGCCGGCTGC
GATCCGCTGATCATGGCCGAGGCGGCGCGGCGGGTTGAAGGCGCCGGAGCCGAGCTGATCGATATCAACATGGGCTGCCC
GGTGCGCAAGGTGGTGGGCGGCTTGGGCGGGTCGGCGCTGATGCGCGATCTGCCTTTGGCCGCGCGTATTCTCGAAGCCG
TGGTCGGTGCCGTGACCCTGCCCGTGAGCCTGAAGATGCGCCTGGGCTGGGATGACGCCAGCCGCAACGCGGTCGAGCTG
GCCCGGCTGGCCGAAAGCGCGGGGATCAGCCGGCTGACCGTCCATGGCCGCACCCGGGCCCAGATGTATGACGGTCGCGC
CGACTGGCCGGCCATCGGTCTGGTCAAACGGGCCGTCTCGCTGCCGGTCATCGCCAATGGCGATGTGACGAACCTGTCCG
AGGCCCGCGAGATCCTGGCCGTTACCGGCGCCGATGGCGTGATGATCGGCCGGGCGGCCCTCGGCGCGCCCTGGTTGCCC
GGCGCCATCGCCCGGGCCATCGCCGCCGGGACGCGGGAAACGCCGCCGCCGGCTTTGGCCGAGAGGGCGGCGGTGATGGC
CGCCCACCTCGAAGGCCTGTTGGCCTGCCATGGTCTGGAGCGTGGCCTTAAGACCTCGCGCAAGCATATGGGCTGGTATG
CGCGGACGATGCCGCAAGGCGCCGACTTCCGCGCCGCCTATATGGCCGCCGCCGATGCCGGGGCGGCGCGCCGAGTTCTC
AACCATTTCACGGCGCTGTGGCAGTCGGCCCCGCCGTCCGCATCCCAAGAAAGCCGCCAAAGAGCGGTCTTGAAAAACCC
CACCCCGGAGGACACGTCACCCGACCGCCCGCGAGAAGAGGCCGCTTGA

Upstream 100 bases:

>100_bases
ATAAGCCAAGTCCTTGAAGGACTTGGTTTTTTGCTTTTGTCCCGCCGATGATGACCGCGGGGATCCACAAGCCGCTCGCC
TTTCGAGGATTCGCTTTCGC

Downstream 100 bases:

>100_bases
AGATGGTGAAGATTTCTGCCCTGACCCGGCGCGCGCCGGTTCCCTCGATCGACCCGGCGACGGTGCTGAGCGCCATCGCT
TCGGCGGTGATCTGTGTCGA

Product: dihydrouridine synthase TIM-barrel protein nifR3

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 362; Mature: 361

Protein sequence:

>362_residues
MTPSPAFPVSDPLIMLAPMAGVTDSPFRRLAHDYGCPLSWTEMVASRELLRERARGVDPRLGGGAEAGRAGPFAVQLAGC
DPLIMAEAARRVEGAGAELIDINMGCPVRKVVGGLGGSALMRDLPLAARILEAVVGAVTLPVSLKMRLGWDDASRNAVEL
ARLAESAGISRLTVHGRTRAQMYDGRADWPAIGLVKRAVSLPVIANGDVTNLSEAREILAVTGADGVMIGRAALGAPWLP
GAIARAIAAGTRETPPPALAERAAVMAAHLEGLLACHGLERGLKTSRKHMGWYARTMPQGADFRAAYMAAADAGAARRVL
NHFTALWQSAPPSASQESRQRAVLKNPTPEDTSPDRPREEAA

Sequences:

>Translated_362_residues
MTPSPAFPVSDPLIMLAPMAGVTDSPFRRLAHDYGCPLSWTEMVASRELLRERARGVDPRLGGGAEAGRAGPFAVQLAGC
DPLIMAEAARRVEGAGAELIDINMGCPVRKVVGGLGGSALMRDLPLAARILEAVVGAVTLPVSLKMRLGWDDASRNAVEL
ARLAESAGISRLTVHGRTRAQMYDGRADWPAIGLVKRAVSLPVIANGDVTNLSEAREILAVTGADGVMIGRAALGAPWLP
GAIARAIAAGTRETPPPALAERAAVMAAHLEGLLACHGLERGLKTSRKHMGWYARTMPQGADFRAAYMAAADAGAARRVL
NHFTALWQSAPPSASQESRQRAVLKNPTPEDTSPDRPREEAA
>Mature_361_residues
TPSPAFPVSDPLIMLAPMAGVTDSPFRRLAHDYGCPLSWTEMVASRELLRERARGVDPRLGGGAEAGRAGPFAVQLAGCD
PLIMAEAARRVEGAGAELIDINMGCPVRKVVGGLGGSALMRDLPLAARILEAVVGAVTLPVSLKMRLGWDDASRNAVELA
RLAESAGISRLTVHGRTRAQMYDGRADWPAIGLVKRAVSLPVIANGDVTNLSEAREILAVTGADGVMIGRAALGAPWLPG
AIARAIAAGTRETPPPALAERAAVMAAHLEGLLACHGLERGLKTSRKHMGWYARTMPQGADFRAAYMAAADAGAARRVLN
HFTALWQSAPPSASQESRQRAVLKNPTPEDTSPDRPREEAA

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family [H]

Homologues:

Organism=Homo sapiens, GI31742496, Length=261, Percent_Identity=33.3333333333333, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI239788462, Length=231, Percent_Identity=31.6017316017316, Blast_Score=105, Evalue=4e-23,
Organism=Homo sapiens, GI239788483, Length=235, Percent_Identity=31.4893617021277, Blast_Score=105, Evalue=7e-23,
Organism=Homo sapiens, GI40807366, Length=227, Percent_Identity=29.9559471365639, Blast_Score=98, Evalue=1e-20,
Organism=Homo sapiens, GI8923374, Length=239, Percent_Identity=29.2887029288703, Blast_Score=84, Evalue=2e-16,
Organism=Escherichia coli, GI1789660, Length=314, Percent_Identity=42.3566878980892, Blast_Score=231, Evalue=7e-62,
Organism=Escherichia coli, GI1788462, Length=315, Percent_Identity=33.015873015873, Blast_Score=139, Evalue=3e-34,
Organism=Escherichia coli, GI145693211, Length=330, Percent_Identity=28.4848484848485, Blast_Score=90, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI25144369, Length=233, Percent_Identity=33.9055793991416, Blast_Score=108, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI17543114, Length=236, Percent_Identity=30.5084745762712, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17507177, Length=229, Percent_Identity=30.5676855895196, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17510279, Length=239, Percent_Identity=29.2887029288703, Blast_Score=75, Evalue=8e-14,
Organism=Saccharomyces cerevisiae, GI6323437, Length=276, Percent_Identity=28.9855072463768, Blast_Score=95, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6323560, Length=313, Percent_Identity=25.2396166134185, Blast_Score=89, Evalue=9e-19,
Organism=Saccharomyces cerevisiae, GI6323433, Length=316, Percent_Identity=23.4177215189873, Blast_Score=76, Evalue=8e-15,
Organism=Drosophila melanogaster, GI24585320, Length=301, Percent_Identity=30.2325581395349, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI19921524, Length=237, Percent_Identity=33.3333333333333, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24580595, Length=230, Percent_Identity=29.5652173913043, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI19920448, Length=230, Percent_Identity=29.5652173913043, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI45549423, Length=245, Percent_Identity=30.6122448979592, Blast_Score=80, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 38114; Mature: 37983

Theoretical pI: Translated: 9.29; Mature: 9.29

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPSPAFPVSDPLIMLAPMAGVTDSPFRRLAHDYGCPLSWTEMVASRELLRERARGVDPR
CCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCC
LGGGAEAGRAGPFAVQLAGCDPLIMAEAARRVEGAGAELIDINMGCPVRKVVGGLGGSAL
CCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHCCCHHHH
MRDLPLAARILEAVVGAVTLPVSLKMRLGWDDASRNAVELARLAESAGISRLTVHGRTRA
HHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCEEEECCCHHH
QMYDGRADWPAIGLVKRAVSLPVIANGDVTNLSEAREILAVTGADGVMIGRAALGAPWLP
HHCCCCCCCCHHHHHHHHHHCCEEECCCCCCHHHHHHEEEEECCCCEEECHHHCCCCCCH
GAIARAIAAGTRETPPPALAERAAVMAAHLEGLLACHGLERGLKTSRKHMGWYARTMPQG
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCC
ADFRAAYMAAADAGAARRVLNHFTALWQSAPPSASQESRQRAVLKNPTPEDTSPDRPREE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCCCCHHC
AA
CC
>Mature Secondary Structure 
TPSPAFPVSDPLIMLAPMAGVTDSPFRRLAHDYGCPLSWTEMVASRELLRERARGVDPR
CCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCC
LGGGAEAGRAGPFAVQLAGCDPLIMAEAARRVEGAGAELIDINMGCPVRKVVGGLGGSAL
CCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHCCCHHHH
MRDLPLAARILEAVVGAVTLPVSLKMRLGWDDASRNAVELARLAESAGISRLTVHGRTRA
HHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCEEEECCCHHH
QMYDGRADWPAIGLVKRAVSLPVIANGDVTNLSEAREILAVTGADGVMIGRAALGAPWLP
HHCCCCCCCCHHHHHHHHHHCCEEECCCCCCHHHHHHEEEEECCCCEEECHHHCCCCCCH
GAIARAIAAGTRETPPPALAERAAVMAAHLEGLLACHGLERGLKTSRKHMGWYARTMPQG
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCC
ADFRAAYMAAADAGAARRVLNHFTALWQSAPPSASQESRQRAVLKNPTPEDTSPDRPREE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCCCCHHC
AA
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]