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The map label for this gene is alkK [H]

Identifier: 83592993

GI number: 83592993

Start: 1948973

End: 1950601

Strand: Direct

Name: alkK [H]

Synonym: Rru_A1658

Alternate gene names: 83592993

Gene position: 1948973-1950601 (Clockwise)

Preceding gene: 83592991

Following gene: 83592994

Centisome position: 44.77

GC content: 62.43

Gene sequence:

>1629_bases
ATGCCGTCTCCCTACAGCACCGGACTTGATCGCACACCCGCCAACCATGTTCCCCTGACGCCGCTGGCCTTCCTTGACAG
GGCGGCGCAGGTTTTCCCAGACCGTCCTTCCCTTGTCCACGGCAAACGCCGCTATACCTGGGCCGAAACGGCCCAGCGCG
CCCGCCGGCTGGCTTCGGCCCTGCGGGCGCGCGGCATCGGCCCCGAGGATACGGTGGCGGTGATGGGCGCCAATACCCCC
GAGCTTTACGAGGCCCATTTCGGCGTGCCGATGGCCGGGGCGGTGCTCAACGCCCTTAACGTTCGCCTCAACGCCGAGGA
GATCGCCTTTATCCTGGAGCATGGCGAGGCGCGCGTTCTGCTGACCGATACCGAGTTTTCGCCGATCATCGCCGACGCCC
TCGCCCGCATCGATCGGCCGATCACGGTGATCGACATCGTCGATCCCGAATACGACGGCCCCGGCGCTTGCGTCGGCGAG
ATGGAGTACGAGGCCTTTCTGGCCGAGGGCGATCCAACCGCCGATTGGCGCTGGCCGGCCGACGAATGGGAATCGATCAC
GCTCAACTATACCTCGGGAACCACCGGCAATCCCAAGGGCGTGGTTTACCACCACCGCGGCGCCTATTTGAACGCGCTTT
CGAACATCATCACCTGGGGGATGCCCCACCATGCCGTTTATCTGTGGACCTTGCCGATGTTCCACTGCAACGGTTGGTGT
TTCCCCTGGACGATGGCGGCCAATGCGGGAACCAACGTCTGCCTGCGCCGGGTGACGGCGGCGGGCATCTTCCAGGCCAT
CGCCGAGGAAAAGGTCAGCCACTTCTGCGGCGCGCCGATCGTGCTGAGCTTTCTGATCAACGCCCGCCCCGAAGAACGGC
GGTCCTTTGATCATCAGGTCAACGTGATGACCGCCGCCGCCCCGCCGCCGGCCTCGGTCCTGGAAAAAATGCAGCGCGAG
GGCTTCTGCGTGACCCATGTGTACGGTCTGACGGAAACCTATGGCCCGGCCACCGTTTGCGCCTGGAAGGAACCGTGGAA
CGACCTGCCGATGGACGAACAGGCGCGGCTGAAATCGCGCCAAGGAGTGCGCTATCTGGTTCAGGAAGGCTTGATGGTCG
GCGATCCGGAAACCATGGAGGCGGTTCCCGCCGACGGCGAAAGCATGGGCGAGGTGTTTTTCCGCGGCAATATCACCATG
AAAGGCTATCTGAAGAATCCCGAGGCCACCGCCGAGGCCTTTCGCGGCGGCTGGTTCCACACCGGGGATCTTGGGGTTCT
TCATCCCGATGGCTATCTTCAGCTCAAGGATCGCTCCAAGGACATCATCATCTCGGGGGGCGAGAATATCTCGTCGATCG
AGGTCGAGGGCGTGCTCCATCGTCACCCTGACGTTGTCGCCGCCGCCGTTGTCGCCAAACCCGATGAGAAATGGGGAGAA
ACCCCTAAAGCTTATGTGGAGTTACGCGACGGCGCTACGGTAACAGAGGATGAACTTGTGGCCTTTTGTCGGCTCCATCT
GGCACATTATAAATGCCCTCGGGATGTTGAGGTGGGGCCGCTGCCAAAAACATCGACGGGTAAGATCCAGAAGTTCGTTC
TGCGCCGGCAGGTGCGAGCGGAAGGATAA

Upstream 100 bases:

>100_bases
TATACGACTCTTTATCGCTCGGGAAAACCGTCGCCGCCGAACCATGCCGGCGAGAACAAGGCGGAGGCCCGTCCAACAGG
CAGGAAAGGTCCCAGCCCAG

Downstream 100 bases:

>100_bases
CCCTCTCTCTGGTCACGCGGTGTGAGCGACGGTATCAGCGGGGGGCGGGCGGCGTCGTGCGGTTGAACCGGCAGACAACT
GGAAACGGATCTGGGAGCCA

Product: acyl-CoA synthetase

Products: D-carnitinyl-CoA; crotonobetainyl-CoA; L-carnitinyl-CoA [C]

Alternate protein names: Medium-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 542; Mature: 541

Protein sequence:

>542_residues
MPSPYSTGLDRTPANHVPLTPLAFLDRAAQVFPDRPSLVHGKRRYTWAETAQRARRLASALRARGIGPEDTVAVMGANTP
ELYEAHFGVPMAGAVLNALNVRLNAEEIAFILEHGEARVLLTDTEFSPIIADALARIDRPITVIDIVDPEYDGPGACVGE
MEYEAFLAEGDPTADWRWPADEWESITLNYTSGTTGNPKGVVYHHRGAYLNALSNIITWGMPHHAVYLWTLPMFHCNGWC
FPWTMAANAGTNVCLRRVTAAGIFQAIAEEKVSHFCGAPIVLSFLINARPEERRSFDHQVNVMTAAAPPPASVLEKMQRE
GFCVTHVYGLTETYGPATVCAWKEPWNDLPMDEQARLKSRQGVRYLVQEGLMVGDPETMEAVPADGESMGEVFFRGNITM
KGYLKNPEATAEAFRGGWFHTGDLGVLHPDGYLQLKDRSKDIIISGGENISSIEVEGVLHRHPDVVAAAVVAKPDEKWGE
TPKAYVELRDGATVTEDELVAFCRLHLAHYKCPRDVEVGPLPKTSTGKIQKFVLRRQVRAEG

Sequences:

>Translated_542_residues
MPSPYSTGLDRTPANHVPLTPLAFLDRAAQVFPDRPSLVHGKRRYTWAETAQRARRLASALRARGIGPEDTVAVMGANTP
ELYEAHFGVPMAGAVLNALNVRLNAEEIAFILEHGEARVLLTDTEFSPIIADALARIDRPITVIDIVDPEYDGPGACVGE
MEYEAFLAEGDPTADWRWPADEWESITLNYTSGTTGNPKGVVYHHRGAYLNALSNIITWGMPHHAVYLWTLPMFHCNGWC
FPWTMAANAGTNVCLRRVTAAGIFQAIAEEKVSHFCGAPIVLSFLINARPEERRSFDHQVNVMTAAAPPPASVLEKMQRE
GFCVTHVYGLTETYGPATVCAWKEPWNDLPMDEQARLKSRQGVRYLVQEGLMVGDPETMEAVPADGESMGEVFFRGNITM
KGYLKNPEATAEAFRGGWFHTGDLGVLHPDGYLQLKDRSKDIIISGGENISSIEVEGVLHRHPDVVAAAVVAKPDEKWGE
TPKAYVELRDGATVTEDELVAFCRLHLAHYKCPRDVEVGPLPKTSTGKIQKFVLRRQVRAEG
>Mature_541_residues
PSPYSTGLDRTPANHVPLTPLAFLDRAAQVFPDRPSLVHGKRRYTWAETAQRARRLASALRARGIGPEDTVAVMGANTPE
LYEAHFGVPMAGAVLNALNVRLNAEEIAFILEHGEARVLLTDTEFSPIIADALARIDRPITVIDIVDPEYDGPGACVGEM
EYEAFLAEGDPTADWRWPADEWESITLNYTSGTTGNPKGVVYHHRGAYLNALSNIITWGMPHHAVYLWTLPMFHCNGWCF
PWTMAANAGTNVCLRRVTAAGIFQAIAEEKVSHFCGAPIVLSFLINARPEERRSFDHQVNVMTAAAPPPASVLEKMQREG
FCVTHVYGLTETYGPATVCAWKEPWNDLPMDEQARLKSRQGVRYLVQEGLMVGDPETMEAVPADGESMGEVFFRGNITMK
GYLKNPEATAEAFRGGWFHTGDLGVLHPDGYLQLKDRSKDIIISGGENISSIEVEGVLHRHPDVVAAAVVAKPDEKWGET
PKAYVELRDGATVTEDELVAFCRLHLAHYKCPRDVEVGPLPKTSTGKIQKFVLRRQVRAEG

Specific function: Could Catalyze The Transfer Of CoA To Crotonobetaine Or Carnitine. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=569, Percent_Identity=27.4165202108963, Blast_Score=168, Evalue=1e-41,
Organism=Homo sapiens, GI187761345, Length=561, Percent_Identity=27.4509803921569, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI187761343, Length=561, Percent_Identity=27.4509803921569, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI122937307, Length=537, Percent_Identity=27.0018621973929, Blast_Score=126, Evalue=4e-29,
Organism=Homo sapiens, GI157311624, Length=538, Percent_Identity=27.3234200743494, Blast_Score=125, Evalue=7e-29,
Organism=Homo sapiens, GI157311622, Length=538, Percent_Identity=27.3234200743494, Blast_Score=125, Evalue=7e-29,
Organism=Homo sapiens, GI42544132, Length=526, Percent_Identity=25.0950570342205, Blast_Score=125, Evalue=9e-29,
Organism=Homo sapiens, GI58082049, Length=536, Percent_Identity=27.0522388059701, Blast_Score=125, Evalue=1e-28,
Organism=Homo sapiens, GI38505220, Length=513, Percent_Identity=25.9259259259259, Blast_Score=117, Evalue=3e-26,
Organism=Homo sapiens, GI115511026, Length=528, Percent_Identity=24.2424242424242, Blast_Score=102, Evalue=9e-22,
Organism=Homo sapiens, GI28416953, Length=529, Percent_Identity=22.4952741020794, Blast_Score=87, Evalue=5e-17,
Organism=Homo sapiens, GI227499619, Length=546, Percent_Identity=22.7106227106227, Blast_Score=82, Evalue=2e-15,
Organism=Homo sapiens, GI42794760, Length=175, Percent_Identity=32, Blast_Score=68, Evalue=2e-11,
Organism=Homo sapiens, GI42794758, Length=175, Percent_Identity=32, Blast_Score=68, Evalue=2e-11,
Organism=Homo sapiens, GI42794756, Length=175, Percent_Identity=32, Blast_Score=68, Evalue=2e-11,
Organism=Homo sapiens, GI40807491, Length=98, Percent_Identity=40.8163265306122, Blast_Score=67, Evalue=5e-11,
Organism=Escherichia coli, GI221142682, Length=509, Percent_Identity=27.3084479371316, Blast_Score=164, Evalue=2e-41,
Organism=Escherichia coli, GI145693145, Length=532, Percent_Identity=27.4436090225564, Blast_Score=151, Evalue=8e-38,
Organism=Escherichia coli, GI1788107, Length=548, Percent_Identity=26.2773722627737, Blast_Score=148, Evalue=7e-37,
Organism=Escherichia coli, GI1786810, Length=520, Percent_Identity=27.1153846153846, Blast_Score=132, Evalue=8e-32,
Organism=Escherichia coli, GI1788595, Length=378, Percent_Identity=27.2486772486773, Blast_Score=80, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI71994694, Length=573, Percent_Identity=28.4467713787086, Blast_Score=178, Evalue=7e-45,
Organism=Caenorhabditis elegans, GI71994703, Length=573, Percent_Identity=28.4467713787086, Blast_Score=178, Evalue=8e-45,
Organism=Caenorhabditis elegans, GI71994690, Length=573, Percent_Identity=28.4467713787086, Blast_Score=177, Evalue=9e-45,
Organism=Caenorhabditis elegans, GI17560308, Length=548, Percent_Identity=27.7372262773723, Blast_Score=162, Evalue=3e-40,
Organism=Caenorhabditis elegans, GI17559526, Length=507, Percent_Identity=27.810650887574, Blast_Score=147, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI17558820, Length=504, Percent_Identity=28.3730158730159, Blast_Score=145, Evalue=7e-35,
Organism=Caenorhabditis elegans, GI32563687, Length=515, Percent_Identity=26.4077669902913, Blast_Score=135, Evalue=6e-32,
Organism=Caenorhabditis elegans, GI17538037, Length=529, Percent_Identity=25.5198487712665, Blast_Score=122, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI17557194, Length=554, Percent_Identity=25.2707581227437, Blast_Score=119, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI17560140, Length=511, Percent_Identity=23.8747553816047, Blast_Score=110, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17531443, Length=514, Percent_Identity=21.9844357976654, Blast_Score=104, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI71996755, Length=550, Percent_Identity=26.3636363636364, Blast_Score=92, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI17564090, Length=91, Percent_Identity=38.4615384615385, Blast_Score=69, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6319699, Length=495, Percent_Identity=25.8585858585859, Blast_Score=134, Evalue=5e-32,
Organism=Saccharomyces cerevisiae, GI6323182, Length=575, Percent_Identity=24.8695652173913, Blast_Score=99, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24581924, Length=572, Percent_Identity=28.4965034965035, Blast_Score=171, Evalue=1e-42,
Organism=Drosophila melanogaster, GI21355181, Length=526, Percent_Identity=25.4752851711027, Blast_Score=132, Evalue=7e-31,
Organism=Drosophila melanogaster, GI18859661, Length=526, Percent_Identity=26.615969581749, Blast_Score=129, Evalue=6e-30,
Organism=Drosophila melanogaster, GI17933690, Length=598, Percent_Identity=22.742474916388, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24648260, Length=519, Percent_Identity=24.8554913294798, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI45550496, Length=540, Percent_Identity=23.7037037037037, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI45552801, Length=544, Percent_Identity=23.1617647058824, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI21356441, Length=479, Percent_Identity=20.8768267223382, Blast_Score=88, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24648257, Length=544, Percent_Identity=23.8970588235294, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI21356947, Length=483, Percent_Identity=22.7743271221532, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24583465, Length=468, Percent_Identity=21.7948717948718, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24583463, Length=468, Percent_Identity=21.7948717948718, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI281364798, Length=469, Percent_Identity=21.7484008528785, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI281365686, Length=468, Percent_Identity=22.4358974358974, Blast_Score=80, Evalue=5e-15,
Organism=Drosophila melanogaster, GI21358303, Length=492, Percent_Identity=24.1869918699187, Blast_Score=73, Evalue=5e-13,
Organism=Drosophila melanogaster, GI20130357, Length=159, Percent_Identity=28.3018867924528, Blast_Score=72, Evalue=8e-13,
Organism=Drosophila melanogaster, GI24653035, Length=462, Percent_Identity=22.0779220779221, Blast_Score=72, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.3.2.- [C]

Molecular weight: Translated: 59808; Mature: 59676

Theoretical pI: Translated: 5.70; Mature: 5.70

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSPYSTGLDRTPANHVPLTPLAFLDRAAQVFPDRPSLVHGKRRYTWAETAQRARRLASA
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
LRARGIGPEDTVAVMGANTPELYEAHFGVPMAGAVLNALNVRLNAEEIAFILEHGEARVL
HHHCCCCCCCCEEEEECCCCHHHHHHCCCCHHHHHHHHHHEEECHHHHEEEEECCCEEEE
LTDTEFSPIIADALARIDRPITVIDIVDPEYDGPGACVGEMEYEAFLAEGDPTADWRWPA
EECCCCCHHHHHHHHHCCCCEEEEEEECCCCCCCCCEECCCEEEEEEECCCCCCCCCCCC
DEWESITLNYTSGTTGNPKGVVYHHRGAYLNALSNIITWGMPHHAVYLWTLPMFHCNGWC
CCCCEEEEEECCCCCCCCCEEEEECCCHHHHHHHHHHHCCCCCCEEEEEEEHHHHCCCEE
FPWTMAANAGTNVCLRRVTAAGIFQAIAEEKVSHFCGAPIVLSFLINARPEERRSFDHQV
EEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHCCCCEE
NVMTAAAPPPASVLEKMQREGFCVTHVYGLTETYGPATVCAWKEPWNDLPMDEQARLKSR
EEEEECCCCCHHHHHHHHHCCEEEEEEECCCCCCCCEEEEEECCCCCCCCCCHHHHHHHH
QGVRYLVQEGLMVGDPETMEAVPADGESMGEVFFRGNITMKGYLKNPEATAEAFRGGWFH
CCHHHHHHCCCEECCCHHHHCCCCCCCCCCCEEEECCEEEEEEECCCHHHHHHHCCCCEE
TGDLGVLHPDGYLQLKDRSKDIIISGGENISSIEVEGVLHRHPDVVAAAVVAKPDEKWGE
ECCEEEECCCCEEEEECCCCEEEEECCCCCCEEEEEEHHHCCCCCEEEHEEECCCHHHCC
TPKAYVELRDGATVTEDELVAFCRLHLAHYKCPRDVEVGPLPKTSTGKIQKFVLRRQVRA
CCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHC
EG
CC
>Mature Secondary Structure 
PSPYSTGLDRTPANHVPLTPLAFLDRAAQVFPDRPSLVHGKRRYTWAETAQRARRLASA
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
LRARGIGPEDTVAVMGANTPELYEAHFGVPMAGAVLNALNVRLNAEEIAFILEHGEARVL
HHHCCCCCCCCEEEEECCCCHHHHHHCCCCHHHHHHHHHHEEECHHHHEEEEECCCEEEE
LTDTEFSPIIADALARIDRPITVIDIVDPEYDGPGACVGEMEYEAFLAEGDPTADWRWPA
EECCCCCHHHHHHHHHCCCCEEEEEEECCCCCCCCCEECCCEEEEEEECCCCCCCCCCCC
DEWESITLNYTSGTTGNPKGVVYHHRGAYLNALSNIITWGMPHHAVYLWTLPMFHCNGWC
CCCCEEEEEECCCCCCCCCEEEEECCCHHHHHHHHHHHCCCCCCEEEEEEEHHHHCCCEE
FPWTMAANAGTNVCLRRVTAAGIFQAIAEEKVSHFCGAPIVLSFLINARPEERRSFDHQV
EEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHCCCCEE
NVMTAAAPPPASVLEKMQREGFCVTHVYGLTETYGPATVCAWKEPWNDLPMDEQARLKSR
EEEEECCCCCHHHHHHHHHCCEEEEEEECCCCCCCCEEEEEECCCCCCCCCCHHHHHHHH
QGVRYLVQEGLMVGDPETMEAVPADGESMGEVFFRGNITMKGYLKNPEATAEAFRGGWFH
CCHHHHHHCCCEECCCHHHHCCCCCCCCCCCEEEECCEEEEEEECCCHHHHHHHCCCCEE
TGDLGVLHPDGYLQLKDRSKDIIISGGENISSIEVEGVLHRHPDVVAAAVVAKPDEKWGE
ECCEEEECCCCEEEEECCCCEEEEECCCCCCEEEEEEHHHCCCCCEEEHEEECCCHHHCC
TPKAYVELRDGATVTEDELVAFCRLHLAHYKCPRDVEVGPLPKTSTGKIQKFVLRRQVRA
CCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHC
EG
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Coenzyme A; D-carnitine; Coenzyme A; crotono-betaine; L-carnitine [C]

Specific reaction: Coenzyme A + D-carnitine = D-carnitinyl-CoA Coenzyme A + crotono-betaine = crotonobetainyl-CoA Coenzyme A + L-carnitine = L-carnitinyl-CoA [C]

General reaction: Ligases; Forming Carbon-Sulfur Bonds; Acid-Thiol Ligases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1453953 [H]