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The map label for this gene is yfgF [C]

Identifier: 83592994

GI number: 83592994

Start: 1950756

End: 1952378

Strand: Direct

Name: yfgF [C]

Synonym: Rru_A1659

Alternate gene names: 83592994

Gene position: 1950756-1952378 (Clockwise)

Preceding gene: 83592993

Following gene: 83592995

Centisome position: 44.82

GC content: 63.59

Gene sequence:

>1623_bases
ATGCAGATCGACAGCCGCGATCGCATCGTTTTTGCCGCCGGTCCTTTCGAGGCGTTCATCGATCGCCGGCAAGACGACCT
GCGCGGCGAGGAGGTCGCGACCCTGATGGTGCCCGAGGACCGGGTGCTGATGGGCCAGTGTCTGAAGTTGGTGCGGCGCA
AAGGGCGGGCCTCCGGCGAGGTGGTCCGCCTTTACCGTCCCCGGGGGGCGCCGCTCTCCATGGACTTCGCCGGCTACACC
CTTGATCAACAAAGCTTCATCGCCTTGCGCCTCACCTCTCACCGTGGGTATGGCGCGCGGGACGGGATCCCCCGCGACCC
GCAATCGGGGCTGCTTCCCCCCCAGGCCTTCAGCGAGCTTGCCAGCGGCAAGATCAAGCAGAGCCAGGAGACGGGGCAGG
ATTTTGGCGTCACCGTCATCGATCTGCCCGGCCTGGGCGGGCTGTGTGACCGTCTGCCCTCTCCGGGCGCCCTTGGCCTT
TTGCGTTCGGTGGGGGCGAGTTTGCGGGCCTGCGCCTTCGATGACAACAGCGCCACCGAAATCGCCCAGGGACGCTATGG
GCTGGTTCACGATGGCGCCGCCGATCTCGAGGCCCTGCGCCACGATCTGTCTGAAATCGTCCGCCAGCATGATCCCGACG
GCGAGGAGGGGCGGGTTGAAACCTCGGCCATCGATCTTGAGGGGATCGACCAGATCGACGAAGGCGATCTGGCCAAGGGC
TTGATCTACGCCATCAATCAGTGCCAGCGCGCCGACGGCGCGGCGATCAGCCTGCGCATGCTGTCAACGAGCATCCCCCA
ACTGGTCCACCAGGGGATCAACGAGGTCGCGCTGTTTCGCCGGGTGGTCGCCAACCGCAGCTTCGATATTGTCCTTCAAC
CGATCGTCAGCCTATCGACGGGCGATATCCACCATTACGAGGCGCTGTGCCGCTTCGACTCCAGCCGCCCCGATCTGTCG
CCCTATCGCGCTATTACCTTCGCCGAGGAAACCGGCCTGATCCACGATTTCGATCTGGCGATGGCCGAAAAGGTGCTGGG
CTGGCTGTCGGGCAAGCCGCGCAATTCCGACGCCTATCGGGTGGCGGTCAATATCTCGGGGTTTTCCATCGGCGTGGACA
GCTATGTGAAGGGGTTGCTCGATCTGCTGCGCGCCGAGCCGTGGACCAAGGGCAAGATGCTGTTCGAGATCACCGAATCC
TCGAAGATGACCGATCTCGATTCCGCTAACAGCTTTATCGGCAGCCTGCGCGACAGCGGCTATCAGGTCTGTCTGGATGA
TTTCGGCGCCGGAGCGGCCAGTTTCCAGTATCTGAGCGCCCTTGAGGTCGATGTGGTCAAGATCGACGGCAGCGCCGTTC
GCAACGCCATGCGCGGCCCCAAGGGACTGGCCTTTCTCAGCGCCCTGACCGAATTATGTCGGCGCCTGGGGGTGACGACC
ATCGCCGAAATGATCGACACCAAGGAAAGTCTTGATTTCGTGCGCAGTTGCGGCTGCGATTTCGTTCAAGGCTATCTGTT
CGGCCGTCCCTCGGCCGATCTCAAGTCGTTCCAGCCGTTACCCAATCTCGGGCTGTTGCGCGGCTCGCCCGACCGCTTGC
GCGGCCGCGCCGGTCTGTCGTAA

Upstream 100 bases:

>100_bases
TCGTGCGGTTGAACCGGCAGACAACTGGAAACGGATCTGGGAGCCACGGAACGAGCGCGACCGCTATGCCGCCCTGGCGT
TTTGTTGGGCCGATCTCATT

Downstream 100 bases:

>100_bases
GGGGGATCGGATGGCGGAGCGTTACAAGGGCGGGCGCGGCGCGTCCCGGCATCGGCTGGCGATGGGGGTTTGGGCGGTCT
TGGCCGGGGCGTTGATCGTC

Product: diguanylate phosphodiesterase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 540; Mature: 540

Protein sequence:

>540_residues
MQIDSRDRIVFAAGPFEAFIDRRQDDLRGEEVATLMVPEDRVLMGQCLKLVRRKGRASGEVVRLYRPRGAPLSMDFAGYT
LDQQSFIALRLTSHRGYGARDGIPRDPQSGLLPPQAFSELASGKIKQSQETGQDFGVTVIDLPGLGGLCDRLPSPGALGL
LRSVGASLRACAFDDNSATEIAQGRYGLVHDGAADLEALRHDLSEIVRQHDPDGEEGRVETSAIDLEGIDQIDEGDLAKG
LIYAINQCQRADGAAISLRMLSTSIPQLVHQGINEVALFRRVVANRSFDIVLQPIVSLSTGDIHHYEALCRFDSSRPDLS
PYRAITFAEETGLIHDFDLAMAEKVLGWLSGKPRNSDAYRVAVNISGFSIGVDSYVKGLLDLLRAEPWTKGKMLFEITES
SKMTDLDSANSFIGSLRDSGYQVCLDDFGAGAASFQYLSALEVDVVKIDGSAVRNAMRGPKGLAFLSALTELCRRLGVTT
IAEMIDTKESLDFVRSCGCDFVQGYLFGRPSADLKSFQPLPNLGLLRGSPDRLRGRAGLS

Sequences:

>Translated_540_residues
MQIDSRDRIVFAAGPFEAFIDRRQDDLRGEEVATLMVPEDRVLMGQCLKLVRRKGRASGEVVRLYRPRGAPLSMDFAGYT
LDQQSFIALRLTSHRGYGARDGIPRDPQSGLLPPQAFSELASGKIKQSQETGQDFGVTVIDLPGLGGLCDRLPSPGALGL
LRSVGASLRACAFDDNSATEIAQGRYGLVHDGAADLEALRHDLSEIVRQHDPDGEEGRVETSAIDLEGIDQIDEGDLAKG
LIYAINQCQRADGAAISLRMLSTSIPQLVHQGINEVALFRRVVANRSFDIVLQPIVSLSTGDIHHYEALCRFDSSRPDLS
PYRAITFAEETGLIHDFDLAMAEKVLGWLSGKPRNSDAYRVAVNISGFSIGVDSYVKGLLDLLRAEPWTKGKMLFEITES
SKMTDLDSANSFIGSLRDSGYQVCLDDFGAGAASFQYLSALEVDVVKIDGSAVRNAMRGPKGLAFLSALTELCRRLGVTT
IAEMIDTKESLDFVRSCGCDFVQGYLFGRPSADLKSFQPLPNLGLLRGSPDRLRGRAGLS
>Mature_540_residues
MQIDSRDRIVFAAGPFEAFIDRRQDDLRGEEVATLMVPEDRVLMGQCLKLVRRKGRASGEVVRLYRPRGAPLSMDFAGYT
LDQQSFIALRLTSHRGYGARDGIPRDPQSGLLPPQAFSELASGKIKQSQETGQDFGVTVIDLPGLGGLCDRLPSPGALGL
LRSVGASLRACAFDDNSATEIAQGRYGLVHDGAADLEALRHDLSEIVRQHDPDGEEGRVETSAIDLEGIDQIDEGDLAKG
LIYAINQCQRADGAAISLRMLSTSIPQLVHQGINEVALFRRVVANRSFDIVLQPIVSLSTGDIHHYEALCRFDSSRPDLS
PYRAITFAEETGLIHDFDLAMAEKVLGWLSGKPRNSDAYRVAVNISGFSIGVDSYVKGLLDLLRAEPWTKGKMLFEITES
SKMTDLDSANSFIGSLRDSGYQVCLDDFGAGAASFQYLSALEVDVVKIDGSAVRNAMRGPKGLAFLSALTELCRRLGVTT
IAEMIDTKESLDFVRSCGCDFVQGYLFGRPSADLKSFQPLPNLGLLRGSPDRLRGRAGLS

Specific function: Unknown

COG id: COG2200

COG function: function code T; FOG: EAL domain

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 MHYT domain [H]

Homologues:

Organism=Escherichia coli, GI1788849, Length=244, Percent_Identity=30.7377049180328, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI87081921, Length=254, Percent_Identity=29.1338582677165, Blast_Score=111, Evalue=1e-25,
Organism=Escherichia coli, GI87081845, Length=235, Percent_Identity=26.8085106382979, Blast_Score=99, Evalue=6e-22,
Organism=Escherichia coli, GI1786507, Length=228, Percent_Identity=28.9473684210526, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI87082096, Length=204, Percent_Identity=25.4901960784314, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI226510982, Length=300, Percent_Identity=28, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1790496, Length=254, Percent_Identity=26.7716535433071, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI87081743, Length=232, Percent_Identity=28.448275862069, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1787541, Length=233, Percent_Identity=28.3261802575107, Blast_Score=91, Evalue=3e-19,
Organism=Escherichia coli, GI87081980, Length=238, Percent_Identity=26.0504201680672, Blast_Score=79, Evalue=1e-15,
Organism=Escherichia coli, GI1788502, Length=239, Percent_Identity=25.9414225941423, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1787055, Length=227, Percent_Identity=25.9911894273128, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1787410, Length=134, Percent_Identity=29.1044776119403, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR001633
- InterPro:   IPR005330 [H]

Pfam domain/function: PF00563 EAL; PF00990 GGDEF; PF03707 MHYT [H]

EC number: NA

Molecular weight: Translated: 58752; Mature: 58752

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: PS50883 EAL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQIDSRDRIVFAAGPFEAFIDRRQDDLRGEEVATLMVPEDRVLMGQCLKLVRRKGRASGE
CCCCCCCCEEEEECCHHHHHHCCHHHCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCC
VVRLYRPRGAPLSMDFAGYTLDQQSFIALRLTSHRGYGARDGIPRDPQSGLLPPQAFSEL
EEEEECCCCCCEEECCCCCEECCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHH
ASGKIKQSQETGQDFGVTVIDLPGLGGLCDRLPSPGALGLLRSVGASLRACAFDDNSATE
HCCCCHHHHHCCCCCCEEEEECCCCCHHHHCCCCCCHHHHHHHHCCHHEEEECCCCCHHH
IAQGRYGLVHDGAADLEALRHDLSEIVRQHDPDGEEGRVETSAIDLEGIDQIDEGDLAKG
HHCCCCCEEECCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCCCHHHHH
LIYAINQCQRADGAAISLRMLSTSIPQLVHQGINEVALFRRVVANRSFDIVLQPIVSLST
HHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCHHHHHHHHHHCCC
GDIHHYEALCRFDSSRPDLSPYRAITFAEETGLIHDFDLAMAEKVLGWLSGKPRNSDAYR
CCHHHHHHHHHCCCCCCCCCCCCEEEEEHHCCCEEHHHHHHHHHHHHHHCCCCCCCCCEE
VAVNISGFSIGVDSYVKGLLDLLRAEPWTKGKMLFEITESSKMTDLDSANSFIGSLRDSG
EEEEECCEEECHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCC
YQVCLDDFGAGAASFQYLSALEVDVVKIDGSAVRNAMRGPKGLAFLSALTELCRRLGVTT
CEEEHHHCCCCHHHHHHHHHCEEEEEEECHHHHHHHHCCCCHHHHHHHHHHHHHHHCHHH
IAEMIDTKESLDFVRSCGCDFVQGYLFGRPSADLKSFQPLPNLGLLRGSPDRLRGRAGLS
HHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCHHHCCCCCCCCCCCCCHHHHCCCCCCC
>Mature Secondary Structure
MQIDSRDRIVFAAGPFEAFIDRRQDDLRGEEVATLMVPEDRVLMGQCLKLVRRKGRASGE
CCCCCCCCEEEEECCHHHHHHCCHHHCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCC
VVRLYRPRGAPLSMDFAGYTLDQQSFIALRLTSHRGYGARDGIPRDPQSGLLPPQAFSEL
EEEEECCCCCCEEECCCCCEECCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHH
ASGKIKQSQETGQDFGVTVIDLPGLGGLCDRLPSPGALGLLRSVGASLRACAFDDNSATE
HCCCCHHHHHCCCCCCEEEEECCCCCHHHHCCCCCCHHHHHHHHCCHHEEEECCCCCHHH
IAQGRYGLVHDGAADLEALRHDLSEIVRQHDPDGEEGRVETSAIDLEGIDQIDEGDLAKG
HHCCCCCEEECCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCCCHHHHH
LIYAINQCQRADGAAISLRMLSTSIPQLVHQGINEVALFRRVVANRSFDIVLQPIVSLST
HHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCHHHHHHHHHHCCC
GDIHHYEALCRFDSSRPDLSPYRAITFAEETGLIHDFDLAMAEKVLGWLSGKPRNSDAYR
CCHHHHHHHHHCCCCCCCCCCCCEEEEEHHCCCEEHHHHHHHHHHHHHHCCCCCCCCCEE
VAVNISGFSIGVDSYVKGLLDLLRAEPWTKGKMLFEITESSKMTDLDSANSFIGSLRDSG
EEEEECCEEECHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCC
YQVCLDDFGAGAASFQYLSALEVDVVKIDGSAVRNAMRGPKGLAFLSALTELCRRLGVTT
CEEEHHHCCCCHHHHHHHHHCEEEEEEECHHHHHHHHCCCCHHHHHHHHHHHHHHHCHHH
IAEMIDTKESLDFVRSCGCDFVQGYLFGRPSADLKSFQPLPNLGLLRGSPDRLRGRAGLS
HHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCHHHCCCCCCCCCCCCCHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]